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Home / Cells / Primary Cells

Diabetic Mouse Coronary Artery Fibroblasts

SKU : BHC16400911

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Cell Biologics, Inc.

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Mfr.No.	MD-6049CO
Description	Diabetic MouseCoronary Artery Fibroblasts fromCell Biologics are isolated from the coronary artery of Diabetic (db/db) mice(8 weeks).Diabetic MouseCoronary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	Diabetic Mouse Coronary Artery Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Frozen	0.5×10⁶cells
General Information	Diabetic Mouse Coronary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics . Repeated freezing and thawing of cells is not recommended.
Tissue	Coronary Artery

Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400911	MD-6049CO	Frozen Vial	$590		Add to Cart

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