RecA from E. coli is a DNA-binding protein that is involved in homologous recombination in an ATP-dependent process. RecA binds to single-stranded DNA forming a nucleoprotein complex and promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. RecA also plays a role in post-replicative DNA repair mechanisms and in DNA repair and UV-induced mutagenesis. RecA Protein is commonly used to study the molecular mechanisms involved in homologous recombination.
Description
displacement loop mutagenesis ; targeted DNA cleavage ; visualization of DNA with electron microscopy ; library screening with RecA coated probes
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration
2.0 mg/ml
Form
Protein supplied with 10X Reaction Buffer.
Quantity
200 µg (100 µl)
Storage
Store all components at -20 °C.
Shipping Condition
Dry Ice
Storage Condition
-20C
Buffer
Storage Buffer: 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.