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Home / VECTOR &VIRUS / Lentivirus

NANOG Reporter Lentivirus

SKU : BHV19400095

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Mfr.No.	LTV-0104, LTV-0104-1S, LTV-0104-2S, LTV-0104-3S, LTV-0104-4S, LTV-0104-5S, LTV-0104-6S, LTV-0104-15S, LTV-0104-16S
Description	NANOG Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse homeobox protein NANOG transcriptional activity in mammalian cells. The NANOG reporter is constructed with tandem repeats of consensus Nanog DNA-binding elements derived from the Tcf3 promoter to read out Nanog transcriptional activity. Nanog is a key factor in maintaining the self-renewal and pluripotency of embryonic stem cells (ESCs). It functions in concert with other factors such as POU5F1 (Oct-4) and Sox2 to establish ESC identity. NANOG is highly expressed in cancer stem cells and may function as an oncogene to promote carcinogenesis. Nanog cooperates with transcription factors such as STAT3 in LIF/STAT3 signaling and is positively regulated by FGF and TGFbeta/Activin signaling, and negatively regulated by BMP signaling, in the maintenance of human ES cell properties. The NANOG reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation and is ideal for studying NANOG transcriptional activity in difficult-to-transfect cells including primary and/or thawed cells.
Serotype	VSV-G
Reporter	Firefly Luc, RFP, BFP2, GFP
Response Element	NANOG response element
Selection	Blasticidin, Puromycin
Signaling pathway	NANOG Signaling Pathway
Species	Human/mouse
Applications Range	Monitor NANOG transcriptional activity in mammalian cells, screen for activators or inhibitors of NANOG transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Your price	Quantity
BHV19400095-1	LTV-0104-1S	5x10^6	Puromycin	GFP	$595		Add to Cart
BHV19400095-2	LTV-0104-2S	5x10^6	Blasticidin	RFP	$595		Add to Cart
BHV19400095-3	LTV-0104-3S	2x10^6	Puromycin	Firefly Luc	$595		Add to Cart
BHV19400095-4	LTV-0104-4S	2x10^6	Blasticidin	Firefly Luc	$595		Add to Cart
BHV19400095-5	LTV-0104-5S	5x10^6	Blasticidin	GFP	$595		Add to Cart
BHV19400095-6	LTV-0104-6S	5x10^6	Puromycin	RFP	$595		Add to Cart
BHV19400095-7	LTV-0104-15S	5x10^6	Puromycin	BFP2	$595		Add to Cart
BHV19400095-8	LTV-0104-16S	5x10^6	Blasticidin	BFP2	$595		Add to Cart

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