PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 17 bands, ranging from 50 bp – 1.5 kb, for molecular weight standards in agarose gel electrophoresis. The 200 and 500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
Suggested-load
Resolves into 17 sharp, tight bands.Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Bands
17 bands
Quantity
500 µl/100 loads
Range
50bp-1.5kb
Reaction
Marker
Reference
Truc-Chi, LH et al. "Evaluate the Efficiency of AGILENT SureDirect Blood PCR Kit." IFMBE :235-241 (2017). DOI: 10.1007/978-981-10-4361-1_39. Application: DNA Markers.