In addition to negative controls, positive controls are also an important part of every experiment to ensure that any reaction abnormalities are not due to reagent or cycler issues. abm’s RT-PCR Control Template Kit is a system including a human RNA template, cDNA template and GAPDH primer mix to incorporate a positive control into existing RT-PCR setups, guaranteed to amplify under the correct conditions. The RNA template can be used to ensure proper cDNA synthesis, while the ready-to-use cDNA template can conveniently be used as an extra control in later PCR reactions, allowing the user to easily add reliable controls at every step of their experiment without hassle. The kit can also be used for teaching and demonstration purposes within student laboratories.
Features:
• Ease of use with a simple set-up
• Suitable for a wide range of RT-PCR assays
• Streamlined protocol suitable for high-throughput applications
Template
RNA
Format
Kit
Quantity
100 rxns
Reaction
Control
Reference
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.