{"title":"DENARASE® toolkit - Enzymatic DNA Removal in Bioprocessing","description":"\u003cp\u003e\u003ca href=\"https:\/\/shop.ebiohippo.com\/pages\/promotions\"\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e\u003c\/a\u003e\u003cspan\u003e is a \u003c\/span\u003e\u003cem\u003eSerratia marcescens\u003c\/em\u003e\u003cspan\u003e-derived endonuclease from c-LEcta, engineered for efficient nucleic acid clearance across GMP biomanufacturing workflows — including viral vector production, vaccine manufacturing, and downstream bioprocessing. The portfolio covers the full salt spectrum: the \u003c\/span\u003e\u003cstrong\u003ewild-type DENARASE\u003csup\u003e®\u003c\/sup\u003e\u003c\/strong\u003e\u003cspan\u003e delivers peak performance at physiological salt conditions (0–150 mM, pH 7.4–9), while \u003c\/span\u003e\u003cstrong\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eHigh Salt\u003c\/strong\u003e\u003cspan\u003e extends activity to 0–500 mM salt — enabling higher viral vector yields in salt-supplemented processes. Both variants are produced free of animal-derived materials, antibiotics, and endotoxin-forming hosts, with R\u0026amp;D and GMP grades offering equivalent technical performance for seamless process scale-up. The companion \u003c\/span\u003e\u003cstrong\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eELISA Kit\u003c\/strong\u003e\u003cspan\u003e provides monoclonal antibody-based quantification of residual endonuclease — covering both wild-type and High Salt variants — for unmatched accuracy and reproducibility in clearance validation.\u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"denarase-bhz16500001","title":"DENARASE®- Precision Nuclease for Cleaner Bioprocessing","description":"\u003cstyle\u003e\n  .bhp-desc { font-size: 16px; color: #1b2f42; line-height: 1.75; max-width: 860px; }\n  .bhp-desc h1 { font-size: 26px; font-weight: 700; color: #b7195d; margin: 0 0 20px; line-height: 1.25; }\n  .bhp-desc h2 { font-size: 20px; font-weight: 700; color: #b7195d; margin: 28px 0 10px; line-height: 1.3; }\n  .bhp-desc h3 { font-size: 18px; font-weight: 700; color: #1a5c58; margin: 24px 0 8px; line-height: 1.3; }\n  .bhp-desc p  { margin: 0 0 14px; }\n  .bhp-desc ul { margin: 10px 0 16px 22px; padding: 0; }\n  .bhp-desc ul li { margin-bottom: 10px; }\n  .bhp-desc sup { font-size: 0.72em; vertical-align: super; }\n  .bhp-desc em  { font-style: italic; }\n  .bhp-desc strong { font-weight: 700; color: #1b2f42; }\n  .bhp-desc hr { border: none; border-top: 1px solid #e2e6ea; margin: 28px 0; }\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch1\u003eAbout DENARASE\u003csup\u003e®\u003c\/sup\u003e\n\u003c\/h1\u003e\n\u003ch3\u003eHow DENARASE\u003csup\u003e®\u003c\/sup\u003e works\u003c\/h3\u003e\n\u003cp\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e specifically hydrolyzes the phosphodiester bonds between nucleotides, leaving smaller fragments of around 3–5 base pairs. The enzyme is active on all forms of nucleic acids including single-stranded, double-stranded, linear, circular, or supercoiled. Best results are obtained when the enzyme is added before the release of DNA.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eProduction of DENARASE\u003csup\u003e®\u003c\/sup\u003e\n\u003c\/h3\u003e\n\u003cp\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e has been developed for use in commercial manufacturing processes of biologicals and complies with EU GMP regulations. The production process uses a gram-positive \u003cem\u003eBacillus\u003c\/em\u003e sp. production host, which minimizes the risks of endotoxins in the product. No antibiotics, Triton X-100, materials with TSE\/BSE risk, or raw materials of animal origin are employed in the manufacture of the product.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e is available in two quality grades\u003c\/h2\u003e\n\u003cp\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e for Research and Development (R\u0026amp;D) use \u0026amp; DENARASE\u003csup\u003e®\u003c\/sup\u003e for manufacturing under GMP.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e \u003cstrong\u003eR\u0026amp;D-grade\u003c\/strong\u003e is produced in conformity with the ISO 9001 standard, with less strict requirements regarding documentation, storage, and distribution.\u003c\/li\u003e\n\u003cli\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e \u003cstrong\u003eGMP-grade\u003c\/strong\u003e is manufactured under EU GMP conditions.\u003c\/li\u003e\n\u003cli\u003eFrom a technical performance perspective, both quality grades are equal and the parameters on the specification are the same. This allows for a seamless transition from early R\u0026amp;D stage towards biopharmaceutical manufacturing under GMP.\u003c\/li\u003e\n\u003cli\u003eIn order to avoid mix-ups, packaging units of the same size are indicated differently in the product name of the two quality standards: \u003cstrong\u003e1,000 \/ 5,000 kU\u003c\/strong\u003e for R\u0026amp;D-grade DENARASE\u003csup\u003e®\u003c\/sup\u003e and \u003cstrong\u003e1 \/ 5 MU\u003c\/strong\u003e for GMP-grade DENARASE\u003csup\u003e®\u003c\/sup\u003e.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\n\u003ch1\u003e\u003ciframe width=\"355\" height=\"199\" src=\"https:\/\/www.youtube.com\/embed\/gzurxSqdAcU\" title=\"DENARASE® Endonuclease for AAV \u0026amp; Viral Vector Production | High-Efficiency DNA Removal\"\u003e\u003c\/iframe\u003e\u003c\/h1\u003e\n\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"c-LEcta GmbH","offers":[{"title":"100 kU \/ R\u0026D","offer_id":53273960808813,"sku":"20804-100K","price":599.5,"currency_code":"USD","in_stock":true},{"title":"500 kU \/ R\u0026D","offer_id":53273960841581,"sku":"20804-500K","price":1831.5,"currency_code":"USD","in_stock":true},{"title":"1000 kU \/ R\u0026D","offer_id":53273960874349,"sku":"20804-1000K","price":2673.0,"currency_code":"USD","in_stock":true},{"title":"5000 kU \/ R\u0026D","offer_id":53273960907117,"sku":"20804-5000K","price":8948.5,"currency_code":"USD","in_stock":true},{"title":"1 MU \/ GMP","offer_id":53273960939885,"sku":"20804-1M","price":3156.0,"currency_code":"USD","in_stock":true},{"title":"5 MU \/ GMP","offer_id":53273960972653,"sku":"20804-5M","price":10573.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DENARASE_GMP_1.webp?v=1777580724"},{"product_id":"denarase-high-salt-bhz16500002","title":"DENARASE® High Salt – Salt-Tolerant Nuclease for High-Efficiency DNA Removal","description":"\u003cstyle\u003e\n  .bhp-desc { font-size: 16px; color: #1b2f42; line-height: 1.75; max-width: 860px; }\n  .bhp-desc h1 { font-size: 26px; font-weight: 700; color: #1a5c58; margin: 0 0 20px; line-height: 1.25; }\n  .bhp-desc h2 { font-size: 20px; font-weight: 700; color: #1a5c58; margin: 28px 0 10px; line-height: 1.3; }\n  .bhp-desc h3 { font-size: 18px; font-weight: 700; color: #b7195d; margin: 24px 0 8px; line-height: 1.3; }\n  .bhp-desc p  { margin: 0 0 14px; }\n  .bhp-desc ul { margin: 10px 0 16px 22px; padding: 0; }\n  .bhp-desc ul li { margin-bottom: 10px; }\n  .bhp-desc sup { font-size: 0.72em; vertical-align: super; }\n  .bhp-desc em  { font-style: italic; }\n  .bhp-desc strong { font-weight: 700; color: #1b2f42; }\n  .bhp-desc hr { border: none; border-top: 1px solid #e2e6ea; margin: 28px 0; }\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch1\u003eAbout DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt\u003c\/h1\u003e\n\u003ch3\u003eHow DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt works\u003c\/h3\u003e\n\u003cp\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt is an engineered version of the wild-type \u003cem\u003eSerratia marcescens\u003c\/em\u003e endonuclease. By introducing a few targeted amino acid substitutions, the enzyme was optimized for salt tolerance, retaining activity at higher salt concentrations without losing specificity for nucleic acids. DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt exhibits high DNA removal activity across a broad range of salt concentrations (0–500 mM NaCl) and pH levels, providing greater flexibility in bioprocessing and enabling seamless integration into existing workflows.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eProduction of DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt\u003c\/h3\u003e\n\u003cp\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt is produced using a method nearly identical to the established, patented DENARASE\u003csup\u003e®\u003c\/sup\u003e manufacturing process, based on expression in a gram-positive \u003cem\u003eBacillus\u003c\/em\u003e sp. strain. Developed for commercial production of biologicals, the enzyme is manufactured under GMP conditions complying with EU GMP regulations. Produced without antibiotics, Triton X-100, animal-derived or TSE\/BSE risk raw materials.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt is available in two quality grades\u003c\/h2\u003e\n\u003cp\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt for Research and Development (R\u0026amp;D) use \u0026amp; DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt for manufacturing under GMP.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt \u003cstrong\u003eR\u0026amp;D-grade\u003c\/strong\u003e: produced under ISO 9001 standard; less stringent requirements for documentation, storage, and distribution.\u003c\/li\u003e\n\u003cli\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt \u003cstrong\u003eGMP-grade\u003c\/strong\u003e: manufactured under EU GMP conditions; dedicated regulatory support via US FDA Drug Master File.\u003c\/li\u003e\n\u003cli\u003eBoth quality grades are technically equivalent, enabling a seamless transition from R\u0026amp;D to GMP manufacturing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003ciframe title=\"High-Performance Endonuclease for Viral Vector Manufacturing | DENARASE High Salt\" src=\"https:\/\/www.youtube.com\/embed\/BvV5MNNzX6k\" height=\"759\" width=\"1349\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e","brand":"c-LEcta GmbH","offers":[{"title":"25 kU \/ R\u0026D","offer_id":53274322796909,"sku":"22002-25k","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100 kU \/ R\u0026D","offer_id":53274322829677,"sku":"22002-100k","price":913.0,"currency_code":"USD","in_stock":true},{"title":"500 kU \/ R\u0026D","offer_id":53274322862445,"sku":"22002-500k","price":2744.5,"currency_code":"USD","in_stock":true},{"title":"1000 kU \/ R\u0026D","offer_id":53274322895213,"sku":"22002-1000k","price":4015.0,"currency_code":"USD","in_stock":true},{"title":"5000 kU \/ R\u0026D","offer_id":53274322927981,"sku":"22002-5000k","price":13579.5,"currency_code":"USD","in_stock":true},{"title":"1 MU \/ GMP","offer_id":53274322960749,"sku":"22002-1M","price":4767.0,"currency_code":"USD","in_stock":true},{"title":"5 MU \/ GMP","offer_id":53274322993517,"sku":"22002-5M","price":15956.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/250313_DENARASEHighSalt_historyslider.webp?v=1777599399"},{"product_id":"denarase-elisa-kit-the-one-for-all-serratia-marcescens-elisa-kit-bhe16500002","title":"DENARASE® ELISA kit The One-for-all Serratia marcescens ELISA Kit","description":"\u003cstyle\u003e\n  .bhp-desc{font-size:16px;color:#1b2f42;line-height:1.75;max-width:860px}\n  .bhp-desc h1{font-size:26px;font-weight:700;color:#b7195d;margin:0 0 20px}\n  .bhp-desc h2{font-size:20px;font-weight:700;color:#b7195d;margin:28px 0 10px}\n  .bhp-desc h3{font-size:18px;font-weight:700;color:#1a5c58;margin:24px 0 8px}\n  .bhp-desc p{margin:0 0 14px}\n  .bhp-desc ul{margin:10px 0 16px 22px;padding:0}\n  .bhp-desc ul li{margin-bottom:10px}\n  .bhp-desc sup{font-size:.72em;vertical-align:super}\n  .bhp-desc em{font-style:italic}\n  .bhp-desc strong{font-weight:700;color:#1b2f42}\n  .bhp-desc hr{border:none;border-top:1px solid #e2e6ea;margin:28px 0}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch1\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e ELISA Kit — The One-for-all \u003cem\u003eSerratia marcescens\u003c\/em\u003e Endonuclease Detection Kit\u003c\/h1\u003e\n\u003ch3\u003eAbout the DENARASE\u003csup\u003e®\u003c\/sup\u003e ELISA Kit\u003c\/h3\u003e\n\u003cp\u003eThe DENARASE\u003csup\u003e®\u003c\/sup\u003e ELISA Kit is a sandwich ELISA for quantitative in vitro measurement of residual \u003cem\u003eSerratia marcescens\u003c\/em\u003e endonuclease in process-related samples. It detects DENARASE\u003csup\u003e®\u003c\/sup\u003e, DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt, and Benzonase\u003csup\u003e®\u003c\/sup\u003e — providing a unified assay solution for monitoring any \u003cem\u003eS. marcescens\u003c\/em\u003e endonuclease across your downstream bioprocessing workflow.\u003c\/p\u003e\n\u003cp\u003eIntended for research and manufacturing use (process development and quality control). \u003cstrong\u003eNot for diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eDetection Principle\u003c\/h3\u003e\n\u003cp\u003eSandwich ELISA. Samples are incubated in 96-well plates pre-coated with a specific monoclonal capture antibody. Bound endonuclease is detected using a biotin-conjugated monoclonal detector antibody, streptavidin-HRP conjugate, and TMB colorimetric substrate. Absorbance read at OD450.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eKey Performance Parameters\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimit of Detection (LOD):\u003c\/strong\u003e 4 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimit of Quantification (LOQ):\u003c\/strong\u003e 12 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWorking Range:\u003c\/strong\u003e ~32–1,000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e ~130 minutes total incubation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCV (OD450 Assay Control):\u003c\/strong\u003e ≤ 15%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapacity:\u003c\/strong\u003e 480 measurements per kit (5 × 96-well plates)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e15–50× more sensitive\u003c\/strong\u003e than competitor ELISA kits using polyclonal antibodies\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e50% shorter assay time\u003c\/strong\u003e versus competitor kits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e5 × Microtest plates\u003c\/strong\u003e (96-well, pre-coated with monoclonal capture antibody)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing \u0026amp; Dilution Buffer (10×)\u003c\/strong\u003e — 3 × 100 mL + 1 × 20 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e Standard (10 µg\/mL)\u003c\/strong\u003e — 1 × 0.1 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetector Antibody (100×)\u003c\/strong\u003e — Biotin-conjugated monoclonal antibody, 1 × 0.75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Conjugate (100×)\u003c\/strong\u003e — Streptavidin-HRP, 1 × 0.75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSubstrate Solution\u003c\/strong\u003e — TMB One, ready to use, 1 × 75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStop Solution\u003c\/strong\u003e — 0.5 M sulfuric acid, 1 × 75 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eStorage \u0026amp; Stability\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStore at \u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003eShelf life: \u003cstrong\u003e18 months\u003c\/strong\u003e from production under proper storage conditions\u003c\/li\u003e\n\u003cli\u003eShipping temperature may deviate from storage temperature without affecting product quality\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003ciframe title=\"DENARASE® ELISA Kit for Quantification of Residual 𝘚𝘦𝘳𝘳𝘢𝘵𝘪𝘢 𝘮𝘢𝘳𝘤𝘦𝘴𝘤𝘦𝘯𝘴  Endonucleases\" src=\"https:\/\/www.youtube.com\/embed\/tswMimFgAcc\" height=\"759\" width=\"1349\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e","brand":"c-LEcta GmbH","offers":[{"title":"5 pre-coated 96-well microtiter plates (480 measurements)","offer_id":53276380332397,"sku":"41901-1","price":4195.0,"currency_code":"USD","in_stock":true},{"title":"500mL Washing and dilution buffer (10x)","offer_id":53285362467181,"sku":"41901-2","price":78.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DENARASEELISAKit.webp?v=1777658742"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/collections\/DENARASE_toolkit.webp?v=1777916194","url":"https:\/\/www.ebiohippo.com\/collections\/denarase%c2%ae-dna-clearance-in-bioprocessing.oembed","provider":"BioHippo","version":"1.0","type":"link"}