{"title":"Fluorescent Secondary Antibodies","description":"\u003cp\u003eFluorophore-conjugated secondary antibodies for immunofluorescence, ICC and flow cytometry — matched to the host species of your primary antibody.\u003c\/p\u003e","products":[{"product_id":"fitc-goat-anti-mouse-igg-bha13700280","title":"FITC, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"100 uL","offer_id":52905233744237,"sku":"A22110-100UL","price":29.87,"currency_code":"USD","in_stock":true},{"title":"500 uL","offer_id":52905233777005,"sku":"A22110-500UL","price":81.37,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_95f7c1c9-12a6-48c2-9215-76ca2cf9ea0f.jpg?v=1769584225"},{"product_id":"fitc-goat-anti-rabbit-igg-bha13700281","title":"FITC, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"100 uL","offer_id":52905233809773,"sku":"A22120-100UL","price":29.87,"currency_code":"USD","in_stock":true},{"title":"500 uL","offer_id":52905233842541,"sku":"A22120-500UL","price":81.37,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_893ecd5b-ea62-40e1-a4a3-a849fcc3b929.jpg?v=1769584225"},{"product_id":"cy3-goat-anti-mouse-igg-bha13700282","title":"Cy3, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"100 uL","offer_id":52905233908077,"sku":"A22210-100UL","price":29.87,"currency_code":"USD","in_stock":true},{"title":"500 uL","offer_id":52905233940845,"sku":"A22210-500UL","price":81.37,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_64b77503-bd63-4bae-a78b-aaa61747e13b.jpg?v=1769584226"},{"product_id":"cy3-goat-anti-rabbit-igg-bha13700283","title":"Cy3, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"100 uL","offer_id":52905233973613,"sku":"A22220-100UL","price":29.87,"currency_code":"USD","in_stock":true},{"title":"500 uL","offer_id":52905234006381,"sku":"A22220-500UL","price":81.37,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_29f7b8bd-8d98-4c98-a531-dd1b635c720a.jpg?v=1769584227"},{"product_id":"dylight-350-goat-anti-mouse-igg-bha13700284","title":"DyLight 350, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234071917,"sku":"A23010-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_ecb907dd-1f3c-45b4-8423-67e2d5ac7ef4.jpg?v=1769584226"},{"product_id":"dylight-350-goat-anti-rabbit-igg-bha13700285","title":"DyLight 350, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234137453,"sku":"A23020-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_35d558ea-6e42-4572-95d7-6d9f3ed19905.jpg?v=1769584226"},{"product_id":"dylight-405-goat-anti-mouse-igg-bha13700286","title":"DyLight 405, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234301293,"sku":"A23110-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_ec43fd96-d7ca-4029-bf97-f459352e9021.jpg?v=1769584226"},{"product_id":"dylight-405-goat-anti-rabbit-igg-bha13700287","title":"DyLight 405, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234399597,"sku":"A23120-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_7248ad42-5c0e-48ff-bfc6-c0517ac0cd6a.jpg?v=1769584227"},{"product_id":"dylight-488-goat-anti-mouse-igg-bha13700289","title":"DyLight 488, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234465133,"sku":"A23210-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_12987b3a-8d1a-478b-baa3-f0ca0db6d7e9.jpg?v=1769584227"},{"product_id":"dylight-488-goat-anti-rabbit-igg-bha13700290","title":"Dylight 488, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234530669,"sku":"A23220-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_c3b6202d-8a03-4cad-ad64-dfaf4e25c9ef.jpg?v=1769584227"},{"product_id":"dylight-488-rabbit-anti-goat-igg-bha13700291","title":"Dylight 488, Rabbit Anti-Goat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule goat IgG. It also reacts with heavy chains of goat IgG, and light chains of all other goat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234596205,"sku":"A23230-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_ec2da5c2-efb9-40a1-9f04-55b35d1ef3b8.jpg?v=1769584228"},{"product_id":"dylight-488-goat-anti-rat-igg-bha13700292","title":"Dylight 488, Goat Anti-Rat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rat IgG. It also reacts with heavy chains of rat IgG, and light chains of all other rat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234661741,"sku":"A23240-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_51b81380-5e13-416a-ae8e-6dde4ac10490.jpg?v=1769584230"},{"product_id":"dylight-549-goat-anti-mouse-igg-bha13700293","title":"Dylight 549, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234792813,"sku":"A23310-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_2b00b756-d172-4440-bf7e-c87bbc52dbc0.jpg?v=1769584228"},{"product_id":"dylight-549-goat-anti-rabbit-igg-bha13700294","title":"Dylight 549, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234858349,"sku":"A23320-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_df27d3f2-97eb-472d-a717-522fe9b37576.jpg?v=1769584229"},{"product_id":"dylight-549-rabbit-anti-goat-igg-bha13700295","title":"Dylight 549, Rabbit Anti-Goat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. 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It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234923885,"sku":"A23330-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_766cf5b1-6410-4f1b-8dda-d4e38a8b6974.jpg?v=1769584230"},{"product_id":"dylight-549-goat-anti-rat-igg-bha13700296","title":"Dylight 549, Goat Anti-Rat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rat IgG. It also reacts with heavy chains of rat IgG, and light chains of all other rat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905234989421,"sku":"A23340-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_d0f145f2-b5ef-4844-bfde-f9d0e81551a3.jpg?v=1769584229"},{"product_id":"dylight-594-goat-anti-mouse-igg-bha13700297","title":"Dylight 594, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235054957,"sku":"A23410-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_2e078d33-c2cb-4202-9915-3d70579e5a97.jpg?v=1769584230"},{"product_id":"dylight-594-goat-anti-rabbit-igg-bha13700298","title":"Dylight 594, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235120493,"sku":"A23420-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_c2b897cd-9b1d-4e98-ab6d-27683bf35662.jpg?v=1769584230"},{"product_id":"dylight-594-rabbit-anti-goat-igg-bha13700299","title":"Dylight 594, Rabbit Anti-Goat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule goat IgG. It also reacts with heavy chains of goat IgG, and light chains of all other goat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235186029,"sku":"A23430-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_1241631b-e2fe-4a45-b2ea-ae5b52e867b3.jpg?v=1769584231"},{"product_id":"dylight-594-goat-anti-rat-igg-bha13700300","title":"Dylight 594, Goat Anti-Rat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rat IgG. It also reacts with heavy chains of rat IgG, and light chains of all other rat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235251565,"sku":"A23440-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_52591203-44a3-4abe-bd1a-26f1d885f17c.jpg?v=1769584231"},{"product_id":"dylight-649-goat-anti-mouse-igg-bha13700301","title":"Dylight 649, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235317101,"sku":"A23610-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_0cc16abe-eeef-4d60-aa1b-6c9e42c01bc9.jpg?v=1769584231"},{"product_id":"dylight-649-goat-anti-rabbit-igg-bha13700302","title":"Dylight 649, Goat Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235382637,"sku":"A23620-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_e35b0cdd-303c-4e35-85a8-7876d713d7ca.jpg?v=1769584232"},{"product_id":"dylight-649-rabbit-anti-goat-igg-bha13700303","title":"Dylight 649, Rabbit Anti-Goat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule goat IgG. It also reacts with heavy chains of goat IgG, and light chains of all other goat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235448173,"sku":"A23630-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_c183d7c1-08db-404c-a9a6-b0929db91961.jpg?v=1769584232"},{"product_id":"dylight-649-goat-anti-rat-igg-bha13700304","title":"Dylight 649, Goat Anti-Rat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rat IgG. It also reacts with heavy chains of rat IgG, and light chains of all other rat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905235677549,"sku":"A23640-500UL","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dylight-1_fb191a16-ffea-449b-b26a-59983e3c3071.jpg?v=1769584233"},{"product_id":"dylight-680-goat-anti-mouse-igg-bha13700305","title":"Dylight 680, Goat Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with heavy chains of mouse IgG, and light chains of all other mouse immunoglobulins. 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The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. 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It's cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905236037997,"sku":"A24211-500UL","price":225.57,"currency_code":"USD","in_stock":true}]},{"product_id":"ifkine-green-donkey-anti-rabbit-igg-bha13700310","title":"IFKine™ Green Donkey Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background). 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The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum\/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with light chains of all other mouse immunoglobulins. It's cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905236398445,"sku":"A24311-500UL","price":225.57,"currency_code":"USD","in_stock":true}]},{"product_id":"ifkine-red-donkey-anti-mouse-igg-bha13700313","title":"IFKine™ Red Donkey Anti-Mouse IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background). The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum\/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule mouse IgG. It also reacts with light chains of all other mouse immunoglobulins. It's cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905236463981,"sku":"A24411-500UL","price":225.57,"currency_code":"USD","in_stock":true}]},{"product_id":"ifkine-red-donkey-anti-rabbit-igg-bha13700314","title":"IFKine™ Red Donkey Anti-Rabbit IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background). The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum\/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule rabbit IgG. It also reacts with light chains of all other rabbit immunoglobulins. It's cross-reaction with human, mouse, rat, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905236693357,"sku":"A24421-500UL","price":225.57,"currency_code":"USD","in_stock":true}]},{"product_id":"ifkine-red-donkey-anti-goat-igg-bha13715071","title":"IFKine™ Red Donkey Anti-Goat IgG","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background). The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum\/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eAbbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOthers\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe antibody reacts with whole molecule goat IgG. It also reacts with light chains of all other goat immunoglobulins. It's cross-reaction with human, mouse, rat, rabbit, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"500 uL","offer_id":52905236988269,"sku":"A24431-500UL","price":225.57,"currency_code":"USD","in_stock":true}]},{"product_id":"goat-anti-mouse-igg-h-and-l-secondary-antibody-cy3-conjugated-bha21012528","title":"Goat Anti-Mouse IgG (H+L) Secondary Antibody, Cy3 Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGoat Anti-Mouse IgG (H+L) Secondary Antibody, Cy3 Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Mouse IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Goat (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Mouse IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This Cy3 conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human\/bovine\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCy3 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody This Cy3 conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human\/bovine\/rabbit IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631169389,"sku":"BA1031","price":133.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_34f79788-9019-4328-b677-3cd1ac561583.png?v=1785186128"},{"product_id":"affinipure-mouse-anti-rabbit-igg-h-and-l-secondary-antibody-tritc-conjugated-bha21012530","title":"AffiniPure Mouse Anti-Rabbit IgG (H+L) secondary antibody, TRITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAffiniPure Mouse Anti-Rabbit IgG (H+L) secondary antibody, TRITC Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Rabbit IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e E. coli (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Rabbit IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e The antibody was purified from antiserum by immunoaffinity chromatography..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Concentrated, Liquid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This CY3 conjugated Protein A, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eContents (provided):\u003c\/strong\u003e 1 mg\/ml of CY3 conjugated specific antibody; 0.01M PBS, 50% glycerol..\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631267693,"sku":"BA1033-0.5ml","price":154.5,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072635691373,"sku":"BA1033-1ml","price":288.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_2acfba2d-78ce-4a43-8f2d-96197fb3006e.png?v=1785186124"},{"product_id":"affinipure-rabbit-anti-rat-igg-h-and-l-secondary-antibody-cy3-conjugated-bha21012533","title":"AffiniPure Rabbit Anti-Rat IgG (H+L) secondary antibody, CY3 Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAffiniPure Rabbit Anti-Rat IgG (H+L) secondary antibody, CY3 Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Rat IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Rat IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e The antibody was purified from antiserum by immunoaffinity chromatography..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Concentrated, Liquid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e CY3 conjugated antibody is specific for rat IgG, and shows no cross-reactivity with human,bovine,goat IgG..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eContents (provided):\u003c\/strong\u003e 1 mg\/ml of CY3 conjugated specific antibody; 0.01M PBS, 5 mg\/ml BSA, 50% glycerol..\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631300461,"sku":"BA1036-0.5ml","price":133.9,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072635429229,"sku":"BA1036-1ml","price":247.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_af3bd540-e48f-462a-8962-fb3bc833f5ff.png?v=1785186123"},{"product_id":"goat-anti-rabbit-igg-h-and-l-secondary-antibody-cy3-conjugated-bha21012529","title":"Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Cy3 Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Cy3 Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Rabbit IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Goat (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Rabbit IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This Cy3 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse\/bovine IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody This Cy3 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse\/bovine IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631333229,"sku":"BA1032","price":133.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_7d9d17ee-477a-4824-87e3-83d8111a4e34.png?v=1785186126"},{"product_id":"rabbit-anti-goat-igg-h-and-l-secondary-antibody-cy3-conjugated-bha21012531","title":"Rabbit Anti-Goat IgG (H+L) Secondary Antibody, Cy3 Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRabbit Anti-Goat IgG (H+L) Secondary Antibody, Cy3 Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Goat IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Goat IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This Cy3 conjugated antibody is specific for goat IgG and shows no cross-reactivity with human\/rat\/mouse\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody This Cy3 conjugated antibody is specific for goat IgG and shows no cross-reactivity with human\/rat\/mouse\/rabbit IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631693677,"sku":"BA1034","price":133.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_6123ca8b-fed9-4f3e-ac05-7995c5f34274.png?v=1785186127"},{"product_id":"cy3-conjugated-avidin-bha21012534","title":"Cy3 Conjugated Avidin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCy3 Conjugated Avidin is an affinity reagent used to bind or detect antibody- and biotin-based reagents in common immunoassays.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Avidin for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCy3 Conjugated Avidin\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631791981,"sku":"BA1037","price":113.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_e16da9e8-d432-42d6-b877-889d64b37742.png?v=1785186125"},{"product_id":"fluoro647-conjugated-affinipure-goat-anti-mouse-igg-h-and-l-bha21012590","title":"Fluoro647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFluoro647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) is a secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Mouse IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Goat (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Mouse IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This Fluoro647 conjugated antibody is specific for mouse IgG and shows no crossreactivity with human\/bovine\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eBackground (provided):\u003c\/strong\u003e Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e converts primary antibody binding into a chemiluminescent or fluorescent signal for relative protein-level comparisons.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"100 uL","offer_id":53072632054125,"sku":"BA1151","price":61.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_4d677c97-955a-459f-b1ca-fdd53e707519.png?v=1785186127"},{"product_id":"rabbit-anti-human-igg-h-and-l-secondary-antibody-tritc-conjugated-bha21012565","title":"Rabbit Anti-Human IgG (H+L) Secondary Antibody, TRITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRabbit Anti-Human IgG (H+L) Secondary Antibody, TRITC Conjugated is a TRITC conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Human IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Human IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e TRITC provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This TRITC conjugated antibody is specific for human IgG and shows no cross-reactivity with rat\/mouse\/goat\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eTRITC Conjugated Rabbit Anti-human IgG (H+L) secondary antibody This TRITC conjugated antibody is specific for human IgG and shows no cross-reactivity with rat\/mouse\/goat\/rabbit IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632086893,"sku":"BA1092-0.5ml","price":139.05,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072633856365,"sku":"BA1092-1ml","price":288.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_c0c248be-5933-45b3-94b2-0ca12baee046.png?v=1785186127"},{"product_id":"affinipure-rabbit-anti-goat-igg-h-and-l-secondary-antibody-fluoro488-conjugated-bha21012577","title":"AffiniPure Rabbit Anti-Goat IgG (H+L) secondary antibody, Fluoro488 Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAffiniPure Rabbit Anti-Goat IgG (H+L) secondary antibody, Fluoro488 Conjugated is a Fluoro488 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Goat IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Goat IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Fluoro488 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e The antibody was purified from antiserum by immunoaffinity chromatography..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Concentrated, Liquid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This Fluoro 488 conjugated antibody is specific for goat IgG has weak cross reactivity with human IgG and shows no cross reactivity with rat\/mouse IgG..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eContents (provided):\u003c\/strong\u003e 1 mg\/ml of Fluoro488 conjugated specific antibody; 0.01M PBS, 5 mg\/ml BSA, 50% glycerol..\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632283501,"sku":"BA1124-0.5ml","price":154.5,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072634347885,"sku":"BA1124-1ml","price":288.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_4137e86f-7c69-4bfc-845c-5be7b4ea31e2.png?v=1785186127"},{"product_id":"goat-anti-human-iga-secondary-antibody-fitc-conjugated-bha21012572","title":"Goat Anti-Human IgA Secondary Antibody, FITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGoat Anti-Human IgA Secondary Antibody, FITC Conjugated is a FITC conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Human IgA. It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Goat (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Human IgA for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e FITC provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This FITC conjugated antibody is specific for human IgA and shows no cross-reactivity with human IgG\/IgM..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFITC Conjugated Goat Anti-human IgA secondary antibody This FITC conjugated antibody is specific for human IgA and shows no cross-reactivity with human IgG\/IgM.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.25 ml","offer_id":53072632316269,"sku":"BA1112-0.25ml","price":97.85,"currency_code":"USD","in_stock":true},{"title":"0.5 ml","offer_id":53072634478957,"sku":"BA1112-0.5ml","price":185.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_5013a6e3-ebdb-471e-b0e5-0c30b4e24af8.png?v=1785186127"},{"product_id":"fitc-conjugated-avidin-bha21012578","title":"FITC Conjugated Avidin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFITC Conjugated Avidin is an affinity reagent used to bind or detect antibody- and biotin-based reagents in common immunoassays.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Avidin for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e FITC provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFITC Conjugated Avidin\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632349037,"sku":"BA1125-0.5ml","price":139.05,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072634249581,"sku":"BA1125-1ml","price":288.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_c22a1de5-9414-423e-8616-8d2e7d420e23.png?v=1785186127"},{"product_id":"rabbit-anti-goat-igg-h-and-l-secondary-antibody-fitc-conjugated-bha21012571","title":"Rabbit Anti-Goat IgG (H+L) Secondary Antibody, FITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRabbit Anti-Goat IgG (H+L) Secondary Antibody, FITC Conjugated is a FITC conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Goat IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Goat IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e FITC provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This FITC conjugated antibody is specific for goat IgG and shows no cross-reactivity with human\/rat\/mouse\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody This FITC conjugated antibody is specific for goat IgG and shows no cross-reactivity with human\/rat\/mouse\/rabbit IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632512877,"sku":"BA1110-0.5ml","price":82.4,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072634085741,"sku":"BA1110-1ml","price":154.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_1b6c4f09-90fe-4705-93ad-c9d930fdecdd.png?v=1785186127"},{"product_id":"rabbit-anti-human-igg-h-and-l-secondary-antibody-cy3-conjugated-bha21012532","title":"Rabbit Anti-Human IgG (H+L) Secondary Antibody, Cy3 Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRabbit Anti-Human IgG (H+L) Secondary Antibody, Cy3 Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Human IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Human IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This Cy3 conjugated antibody is specific for human IgG and shows no cross-reactivity with rat\/mouse\/goat\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCy3 Conjugated Rabbit Anti-Human IgG (H+L) secondary antibody This Cy3 conjugated antibody is specific for human IgG and shows no cross-reactivity with rat\/mouse\/goat\/rabbit IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632611181,"sku":"BA1035","price":133.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_08d8c76b-5fae-471c-841f-5451b4a6ae02.png?v=1785186127"},{"product_id":"goat-anti-rabbit-igg-h-and-l-secondary-antibody-tritc-conjugated-bha21012563","title":"Goat Anti-Rabbit IgG (H+L) Secondary Antibody, TRITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGoat Anti-Rabbit IgG (H+L) Secondary Antibody, TRITC Conjugated is a TRITC conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Rabbit IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Goat (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Rabbit IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e TRITC provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This TRITC conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse\/bovine IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eTRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody This TRITC conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse\/bovine IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632676717,"sku":"BA1090-0.5ml","price":139.05,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072634708333,"sku":"BA1090-1ml","price":288.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_da260dba-f755-4699-a6f5-4e30310a6bc1.png?v=1785186124"},{"product_id":"goat-anti-human-igm-secondary-antibody-fitc-conjugated-bha21012574","title":"Goat Anti-Human IgM Secondary Antibody, FITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGoat Anti-Human IgM Secondary Antibody, FITC Conjugated is a FITC conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Human IgM. It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Goat (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Human IgM for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e FITC provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This FITC conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA\/IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFITC Conjugated Goat Anti-human IgM secondary antibody This FITC conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA\/IgG.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.25 ml","offer_id":53072632709485,"sku":"BA1116-0.25ml","price":97.85,"currency_code":"USD","in_stock":true},{"title":"0.5 ml","offer_id":53072635494765,"sku":"BA1116-0.5ml","price":185.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Boster_Bio_Box_Placeholder_2072e491-5f52-4a3a-83c2-56b62ac6400d.png?v=1785186124"}],"url":"https:\/\/www.ebiohippo.com\/collections\/fluorescent-secondary-antibodies.oembed","provider":"BioHippo","version":"1.0","type":"link"}