{"title":"Kerry Enzymes","description":"\u003cp\u003eDenarase® nucleases for host-cell DNA clearance, the \u003cem\u003eSerratia marcescens\u003c\/em\u003e ELISA kit, and CellTrypase for animal origin-free cell dissociation — manufactured at the Kerry Biotechnology Centre in Leipzig, Germany and stocked in the United States by BioHippo.\u003c\/p\u003e","products":[{"product_id":"denarase-bhz16500001","title":"Denarase® — Precision Nuclease for Cleaner Bioprocessing","description":"\u003cstyle\u003e .bhp-desc { font-size: 16px; color: #1b2f42; line-height: 1.75; max-width: 860px; } .bhp-desc h1 { font-size: 26px; font-weight: 700; color: #b7195d; margin: 0 0 20px; line-height: 1.25; } .bhp-desc h2 { font-size: 20px; font-weight: 700; color: #b7195d; margin: 28px 0 10px; line-height: 1.3; } .bhp-desc h3 { font-size: 18px; font-weight: 700; color: #1a5c58; margin: 24px 0 8px; line-height: 1.3; } .bhp-desc p  { margin: 0 0 14px; } .bhp-desc ul { margin: 10px 0 16px 22px; padding: 0; } .bhp-desc ul li { margin-bottom: 10px; } .bhp-desc sup { font-size: 0.72em; vertical-align: super; } .bhp-desc em  { font-style: italic; } .bhp-desc strong { font-weight: 700; color: #1b2f42; } .bhp-desc hr { border: none; border-top: 1px solid #e2e6ea; margin: 28px 0; } \u003c\/style\u003e \u003cdiv class=\"bhp-desc\"\u003e \u003ch1\u003eAbout Denarase\u003csup\u003e®\u003c\/sup\u003e \u003c\/h1\u003e \u003ch3\u003eHow Denarase\u003csup\u003e®\u003c\/sup\u003e works\u003c\/h3\u003e \u003cp\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e specifically hydrolyzes the phosphodiester bonds between nucleotides, leaving smaller fragments of around 3–5 base pairs. The enzyme is active on all forms of nucleic acids including single-stranded, double-stranded, linear, circular, or supercoiled. Because of its high activity and relative insensitivity to the substrate form, it is used as a processing aid \/ ancillary material to remove excess nucleic acids in biopharmaceutical production processes.\u003c\/p\u003e \u003chr\u003e \u003ch3\u003eProduction of Denarase\u003csup\u003e®\u003c\/sup\u003e \u003c\/h3\u003e \u003cp\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e has been developed for use in commercial manufacturing processes of biologicals and is produced under GMP conditions according to EU GMP regulations; the c-LEcta quality management system for Denarase\u003csup\u003e®\u003c\/sup\u003e additionally complies with the EXCiPACT\u003csup\u003e®\u003c\/sup\u003e certification standard for GMP\/GDP of pharmaceutical excipients. The production process uses a gram-positive, endotoxin-free \u003cem\u003eBacillus\u003c\/em\u003e sp. production host (a safety-level-S1 GMO; the final product is free of the production strain). No antibiotics, materials with TSE\/BSE risk, or raw materials of animal origin are employed in the manufacture of the product, and Denarase\u003csup\u003e®\u003c\/sup\u003e is manufactured in Germany by c-LEcta GmbH, now part of Kerry.\u003c\/p\u003e \u003chr\u003e \u003ch2\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e is available in two quality grades\u003c\/h2\u003e \u003cp\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e for Research and Development (R\u0026amp;D) use \u0026amp; Denarase\u003csup\u003e®\u003c\/sup\u003e for manufacturing under GMP.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e \u003cstrong\u003eR\u0026amp;D-grade\u003c\/strong\u003e is produced in conformity with the ISO 9001 standard, with less strict requirements regarding documentation, storage, and distribution.\u003c\/li\u003e \u003cli\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e \u003cstrong\u003eGMP-grade\u003c\/strong\u003e is manufactured under EU GMP conditions and is intended for clinical-phase and commercial manufacturing. Regulatory support documentation for US-market submissions is available on request.\u003c\/li\u003e \u003cli\u003eFrom a technical performance perspective, both quality grades are equal and the parameters on the specification are the same. This allows for a seamless transition from early R\u0026amp;D stage towards biopharmaceutical manufacturing under GMP.\u003c\/li\u003e \u003cli\u003eIn order to avoid mix-ups, packaging units of the same size are indicated differently in the product name of the two quality standards: \u003cstrong\u003e1,000 \/ 5,000 kU\u003c\/strong\u003e for R\u0026amp;D-grade Denarase\u003csup\u003e®\u003c\/sup\u003e and \u003cstrong\u003e1 \/ 5 MU\u003c\/strong\u003e for GMP-grade Denarase\u003csup\u003e®\u003c\/sup\u003e.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ciframe width=\"355\" height=\"199\" src=\"https:\/\/www.youtube.com\/embed\/gzurxSqdAcU\" title=\"Denarase® Endonuclease for AAV \u0026amp; Viral Vector Production | High-Efficiency DNA Removal\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e \u003c\/div\u003e ","brand":"Kerry (formerly c-LEcta)","offers":[{"title":"100 kU \/ R\u0026D","offer_id":53273960808813,"sku":"20804-100K","price":599.5,"currency_code":"USD","in_stock":true},{"title":"500 kU \/ R\u0026D","offer_id":53273960841581,"sku":"20804-500K","price":1831.5,"currency_code":"USD","in_stock":true},{"title":"1000 kU \/ R\u0026D","offer_id":53273960874349,"sku":"20804-1000K","price":2673.0,"currency_code":"USD","in_stock":true},{"title":"5000 kU \/ R\u0026D","offer_id":53273960907117,"sku":"20804-5000K","price":8948.5,"currency_code":"USD","in_stock":true},{"title":"1 MU \/ GMP","offer_id":53273960939885,"sku":"20804-1M","price":3156.0,"currency_code":"USD","in_stock":true},{"title":"5 MU \/ GMP","offer_id":53273960972653,"sku":"20804-5M","price":10573.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DENARASE_GMP_1.webp?v=1777580724"},{"product_id":"denarase-high-salt-bhz16500002","title":"Denarase® High Salt – Salt-Tolerant Nuclease for High-Efficiency DNA Removal","description":"\u003cstyle\u003e\n  .bhp-desc { font-size: 16px; color: #1b2f42; line-height: 1.75; max-width: 860px; }\n  .bhp-desc h1 { font-size: 26px; font-weight: 700; color: #1a5c58; margin: 0 0 20px; line-height: 1.25; }\n  .bhp-desc h2 { font-size: 20px; font-weight: 700; color: #1a5c58; margin: 28px 0 10px; line-height: 1.3; }\n  .bhp-desc h3 { font-size: 18px; font-weight: 700; color: #b7195d; margin: 24px 0 8px; line-height: 1.3; }\n  .bhp-desc p  { margin: 0 0 14px; }\n  .bhp-desc ul { margin: 10px 0 16px 22px; padding: 0; }\n  .bhp-desc ul li { margin-bottom: 10px; }\n  .bhp-desc sup { font-size: 0.72em; vertical-align: super; }\n  .bhp-desc em  { font-style: italic; }\n  .bhp-desc strong { font-weight: 700; color: #1b2f42; }\n  .bhp-desc hr { border: none; border-top: 1px solid #e2e6ea; margin: 28px 0; }\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch1\u003eAbout Denarase\u003csup\u003e®\u003c\/sup\u003e High Salt\u003c\/h1\u003e\n\u003ch3\u003eHow Denarase\u003csup\u003e®\u003c\/sup\u003e High Salt works\u003c\/h3\u003e\n\u003cp\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e High Salt is an engineered version of the wild-type \u003cem\u003eSerratia marcescens\u003c\/em\u003e endonuclease. By introducing a few targeted amino acid substitutions, the enzyme was optimized for salt tolerance, retaining activity at higher salt concentrations without losing specificity for nucleic acids. Denarase\u003csup\u003e®\u003c\/sup\u003e High Salt exhibits high DNA removal activity across a broad range of salt concentrations (0–500 mM NaCl) and pH levels, providing greater flexibility in bioprocessing and enabling seamless integration into existing workflows.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eProduction of Denarase\u003csup\u003e®\u003c\/sup\u003e High Salt\u003c\/h3\u003e\n\u003cp\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e High Salt is produced using a method nearly identical to the established, patented Denarase\u003csup\u003e®\u003c\/sup\u003e manufacturing process, based on expression in a gram-positive \u003cem\u003eBacillus\u003c\/em\u003e sp. strain. Developed for commercial production of biologicals, the enzyme is manufactured under GMP conditions complying with EU GMP regulations. Produced without antibiotics, Triton X-100, animal-derived or TSE\/BSE risk raw materials.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e High Salt is available in two quality grades\u003c\/h2\u003e\n\u003cp\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e High Salt for Research and Development (R\u0026amp;D) use \u0026amp; Denarase\u003csup\u003e®\u003c\/sup\u003e High Salt for manufacturing under GMP.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e High Salt \u003cstrong\u003eR\u0026amp;D-grade\u003c\/strong\u003e: produced under ISO 9001 standard; less stringent requirements for documentation, storage, and distribution.\u003c\/li\u003e\n\u003cli\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e High Salt \u003cstrong\u003eGMP-grade\u003c\/strong\u003e: manufactured under EU GMP conditions, with regulatory documentation available on request.\u003c\/li\u003e\n\u003cli\u003eBoth quality grades are technically equivalent, enabling a seamless transition from R\u0026amp;D to GMP manufacturing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003ciframe title=\"High-Performance Endonuclease for Viral Vector Manufacturing | Denarase High Salt\" src=\"https:\/\/www.youtube.com\/embed\/BvV5MNNzX6k\" height=\"759\" width=\"1349\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e","brand":"Kerry (formerly c-LEcta)","offers":[{"title":"25 kU \/ R\u0026D","offer_id":53274322796909,"sku":"22002-25k","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100 kU \/ R\u0026D","offer_id":53274322829677,"sku":"22002-100k","price":913.0,"currency_code":"USD","in_stock":true},{"title":"500 kU \/ R\u0026D","offer_id":53274322862445,"sku":"22002-500k","price":2744.5,"currency_code":"USD","in_stock":true},{"title":"1000 kU \/ R\u0026D","offer_id":53274322895213,"sku":"22002-1000k","price":4015.0,"currency_code":"USD","in_stock":true},{"title":"5000 kU \/ R\u0026D","offer_id":53274322927981,"sku":"22002-5000k","price":13579.5,"currency_code":"USD","in_stock":true},{"title":"1 MU \/ GMP","offer_id":53274322960749,"sku":"22002-1M","price":4767.0,"currency_code":"USD","in_stock":true},{"title":"5 MU \/ GMP","offer_id":53274322993517,"sku":"22002-5M","price":15956.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/250313_DENARASEHighSalt_historyslider.webp?v=1777599399"},{"product_id":"denarase-elisa-kit-the-one-for-all-serratia-marcescens-elisa-kit-bhe16500002","title":"Denarase® ELISA Kit — The One-for-All Serratia marcescens ELISA Kit","description":"\u003cstyle\u003e\n  .bhp-desc{font-size:16px;color:#1b2f42;line-height:1.75;max-width:860px}\n  .bhp-desc h1{font-size:26px;font-weight:700;color:#b7195d;margin:0 0 20px}\n  .bhp-desc h2{font-size:20px;font-weight:700;color:#b7195d;margin:28px 0 10px}\n  .bhp-desc h3{font-size:18px;font-weight:700;color:#1a5c58;margin:24px 0 8px}\n  .bhp-desc p{margin:0 0 14px}\n  .bhp-desc ul{margin:10px 0 16px 22px;padding:0}\n  .bhp-desc ul li{margin-bottom:10px}\n  .bhp-desc sup{font-size:.72em;vertical-align:super}\n  .bhp-desc em{font-style:italic}\n  .bhp-desc strong{font-weight:700;color:#1b2f42}\n  .bhp-desc hr{border:none;border-top:1px solid #e2e6ea;margin:28px 0}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch1\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e ELISA Kit — The One-for-all \u003cem\u003eSerratia marcescens\u003c\/em\u003e Endonuclease Detection Kit\u003c\/h1\u003e\n\u003ch3\u003eAbout the Denarase\u003csup\u003e®\u003c\/sup\u003e ELISA Kit\u003c\/h3\u003e\n\u003cp\u003eThe Denarase\u003csup\u003e®\u003c\/sup\u003e ELISA Kit is a sandwich ELISA for quantitative in vitro measurement of residual \u003cem\u003eSerratia marcescens\u003c\/em\u003e endonuclease in process-related samples. It detects Denarase\u003csup\u003e®\u003c\/sup\u003e, Denarase\u003csup\u003e®\u003c\/sup\u003e High Salt, and Benzonase\u003csup\u003e®\u003c\/sup\u003e — providing a unified assay solution for monitoring any \u003cem\u003eS. marcescens\u003c\/em\u003e endonuclease across your downstream bioprocessing workflow.\u003c\/p\u003e\n\u003cp\u003eIntended for research and manufacturing use (process development and quality control). \u003cstrong\u003eNot for diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eDetection Principle\u003c\/h3\u003e\n\u003cp\u003eSandwich ELISA. Samples are incubated in 96-well plates pre-coated with a specific monoclonal capture antibody. Bound endonuclease is detected using a biotin-conjugated monoclonal detector antibody, streptavidin-HRP conjugate, and TMB colorimetric substrate. Absorbance read at OD450.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eKey Performance Parameters\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimit of Detection (LOD):\u003c\/strong\u003e 4 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimit of Quantification (LOQ):\u003c\/strong\u003e 12 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWorking Range:\u003c\/strong\u003e ~32–1,000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e ~130 minutes total incubation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCV (OD450 Assay Control):\u003c\/strong\u003e ≤ 15%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapacity:\u003c\/strong\u003e 480 measurements per kit (5 × 96-well plates)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e15–50× more sensitive\u003c\/strong\u003e than competitor ELISA kits using polyclonal antibodies\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e50% shorter assay time\u003c\/strong\u003e versus competitor kits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e5 × Microtest plates\u003c\/strong\u003e (96-well, pre-coated with monoclonal capture antibody)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing Buffer (10×)\u003c\/strong\u003e — 3 × 100 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDilution Buffer (10×)\u003c\/strong\u003e — 1 × 20 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDenarase\u003csup\u003e®\u003c\/sup\u003e Standard (10 µg\/mL)\u003c\/strong\u003e — 1 × 0.1 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetector Antibody (100×)\u003c\/strong\u003e — biotin-conjugated monoclonal antibody, 1 × 0.75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Conjugate (100×)\u003c\/strong\u003e — streptavidin-HRP, 1 × 0.75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSubstrate Solution\u003c\/strong\u003e — TMB One, ready to use, 1 × 75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStop Solution\u003c\/strong\u003e — 0.5 M sulfuric acid, 1 × 75 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eStorage \u0026amp; Stability\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStore at \u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003eShelf life: \u003cstrong\u003e18 months\u003c\/strong\u003e from production under proper storage conditions\u003c\/li\u003e\n\u003cli\u003eShipping temperature may deviate from storage temperature without affecting product quality\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003ciframe title=\"Denarase® ELISA Kit for Quantification of Residual Serratia marcescens Endonucleases\" src=\"https:\/\/www.youtube.com\/embed\/tswMimFgAcc\" height=\"759\" width=\"1349\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e","brand":"Kerry (formerly c-LEcta)","offers":[{"title":"5 pre-coated 96-well microtiter plates (480 measurements)","offer_id":53276380332397,"sku":"41901-1","price":4195.0,"currency_code":"USD","in_stock":true},{"title":"500mL Washing and dilution buffer (10x)","offer_id":53285362467181,"sku":"41901-2","price":78.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DENARASEELISAKit.webp?v=1777658742"},{"product_id":"celltrypase-c-lecta-bhz16500003","title":"CellTrypase – Recombinant Trypsin-Like Enzyme for Cell Dissociation","description":"\u003ch2\u003eScientific background\u003c\/h2\u003e\n\u003cp\u003eTrypsin-like serine proteases cleave peptide bonds on the carboxyl side of lysine and arginine residues — the same chemistry exploited during cell dissociation. Conventional porcine trypsin carries the biosafety considerations associated with animal-derived materials, including lot-to-lot variability and the theoretical risk of adventitious agents. Recombinant, non-mammalian alternatives remove the animal origin entirely and are produced under controlled fermentation conditions.\u003c\/p\u003e\n\u003cp\u003eCellTrypase is a recombinant trypsin-like enzyme derived from \u003cem\u003eFusarium oxysporum\u003c\/em\u003e and produced by microbial fermentation using \u003cem\u003eBacillus\u003c\/em\u003e sp. as the expression host. Its fungal origin confers lower non-specific reactivity than mammalian trypsin, and the calculated molecular weight of the single-chain enzyme is approximately 22 kDa\u003csup\u003e1\u003c\/sup\u003e.\u003c\/p\u003e\n\n\u003ch2\u003eProduct description\u003c\/h2\u003e\n\u003cp\u003eCellTrypase is manufactured in-house at the Kerry Biotechnology Centre in Leipzig, Germany, by c-LEcta GmbH — now operating as Kerry (formerly c-LEcta) — under a certified quality management system. It is available in two grades — a GMP grade produced under a QMS compliant with the EXCiPACT® certification standard for pharmaceutical excipients, and an R\u0026amp;D grade manufactured under ISO 9001 — and in two concentration formats, 1× ready-to-use and 10× concentrated stock, supplied in 100 mL and 500 mL bottles. Both grades are made by the same process and are released against the same specification.\u003c\/p\u003e\n\u003cp\u003eThe formulation is a sterile-filtered liquid in PBS with 1.1 mM EDTA. Supplying the enzyme as a liquid removes drying and reconstitution steps, which preserves enzyme integrity and gives a clear, ready-to-use solution.\u003c\/p\u003e\n\n\u003ch2\u003eMeasured dissociation performance\u003c\/h2\u003e\n\u003cp\u003eKerry tested the 1× working solution on four adherent production cell lines over five consecutive passages (n = 3 technical replicates), measuring dissociation time, viability, cell yield and population doubling time against the current industry-standard trypsin-like enzyme as the control. The comparator is not named in the source documents; it is described only as the \"current industry standard trypsin-like enzyme\".\u003c\/p\u003e\n\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth align=\"left\"\u003eCell line\u003c\/th\u003e\n\u003cth align=\"left\"\u003eDissociation time (min:s)\u003cbr\u003e\u003csmall\u003econtrol → CellTrypase\u003c\/small\u003e\n\u003c\/th\u003e\n\u003cth align=\"left\"\u003eMean viability\u003cbr\u003e\u003csmall\u003econtrol → CellTrypase\u003c\/small\u003e\n\u003c\/th\u003e\n\u003cth align=\"left\"\u003eMean yield (% of control)\u003c\/th\u003e\n\u003cth align=\"left\"\u003eDoubling time (h:min)\u003cbr\u003e\u003csmall\u003econtrol → CellTrypase\u003c\/small\u003e\n\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eCHO-K1\u003c\/td\u003e\n\u003ctd\u003e02:30 → 02:25\u003c\/td\u003e\n\u003ctd\u003e97% → 98%\u003c\/td\u003e\n\u003ctd\u003e106%\u003c\/td\u003e\n\u003ctd\u003e15:17 → 14:47\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHEK 293\u003c\/td\u003e\n\u003ctd\u003e02:46 → 02:27\u003c\/td\u003e\n\u003ctd\u003e95% → 95%\u003c\/td\u003e\n\u003ctd\u003e100%\u003c\/td\u003e\n\u003ctd\u003e27:10 → 26:55\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMDCK\u003c\/td\u003e\n\u003ctd\u003e25:23 → 23:32\u003c\/td\u003e\n\u003ctd\u003e99% → 99%\u003c\/td\u003e\n\u003ctd\u003e102%\u003c\/td\u003e\n\u003ctd\u003e19:09 → 18:52\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVero\u003c\/td\u003e\n\u003ctd\u003e03:59 → 04:07\u003c\/td\u003e\n\u003ctd\u003e99% → 99%\u003c\/td\u003e\n\u003ctd\u003e103%\u003c\/td\u003e\n\u003ctd\u003e19:45 → 19:16\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cem\u003eMean viability and yield are averages across five passages (P0–P4); yield is expressed relative to the industry-standard control. MDCK is a tightly adherent line and takes over 20 minutes with either enzyme. Source: Kerry scientific poster \"Advancing cell dissociation in bioprocessing with CellTrypase\" (Feb 2026) and CellTrypase Product Information Sheet v4.0 (Aug 2026).\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eKerry also reports data in human induced pluripotent stem cells (iPSCs), the most sensitive system tested. In 2D colonies, 1× CellTrypase reached single-cell suspension in 5 minutes versus 7 minutes for the control, with 94% viability for both enzymes, 99% relative yield, residual aggregates below 5%, and pluripotency marker expression (Oct3\/4, SSEA4, TRA-1-60, CD30, Nanog, Sox2, SSEA3) above the 75% acceptance threshold by flow cytometry. In 3D iPSC aggregates, both enzymes took about 10 minutes; CellTrypase gave 87% viability (control 85%), 104% relative yield and fewer residual aggregates (12% versus 16%).\u003c\/p\u003e\n\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth align=\"left\"\u003eHuman iPSC format\u003c\/th\u003e\n\u003cth align=\"left\"\u003eDissociation time\u003cbr\u003e\u003csmall\u003econtrol → CellTrypase\u003c\/small\u003e\n\u003c\/th\u003e\n\u003cth align=\"left\"\u003eViability\u003cbr\u003e\u003csmall\u003econtrol → CellTrypase\u003c\/small\u003e\n\u003c\/th\u003e\n\u003cth align=\"left\"\u003eYield (% of control)\u003c\/th\u003e\n\u003cth align=\"left\"\u003eResidual aggregates\u003cbr\u003e\u003csmall\u003econtrol → CellTrypase\u003c\/small\u003e\n\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e2D colonies\u003c\/td\u003e\n\u003ctd\u003e7 min → 5 min\u003c\/td\u003e\n\u003ctd\u003e94% → 94%\u003c\/td\u003e\n\u003ctd\u003e99%\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;5% → \u0026lt;5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3D pluripotent aggregates\u003c\/td\u003e\n\u003ctd\u003e10 min → 10 min\u003c\/td\u003e\n\u003ctd\u003e85% → 87%\u003c\/td\u003e\n\u003ctd\u003e104%\u003c\/td\u003e\n\u003ctd\u003e16% → 12%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cem\u003e1× CellTrypase versus the industry-standard control; mean ± SD of n = 3 technical replicates. Source: Kerry poster, Feb 2026. Data on differentiated 3D iPSC aggregates and the full five-passage figure set for each cell line are in the CellTrypase Application Note v1.1, which Kerry supplies on request — \u003ca href=\"\/pages\/celltrypase-technical-documents\"\u003erequest it here\u003c\/a\u003e.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eCellTrypase requires no trypsin inhibitor for inactivation — quenching by dilution in fresh medium is sufficient. Every lot is released at ≥95% purity by size-exclusion HPLC; the chromatogram of the 10× GMP stock shows a single, well-defined peak, whereas the industry-standard comparator shows a broader peak base with small additional signals (see Validation \u0026amp; QC below).\u003c\/p\u003e\n\n\u003ch2\u003eIntended applications\u003c\/h2\u003e\n\u003cp\u003eKerry specifies CellTrypase for gentle, efficient dissociation of adherent and aggregating cells in biopharmaceutical manufacturing and research, including:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePassaging and expansion of adherent production lines such as CHO-K1, HEK 293, Vero and MDCK\u003c\/li\u003e\n\u003cli\u003eCell dissociation in viral vector and viral vaccine production for cell and gene therapies\u003c\/li\u003e\n\u003cli\u003ePassaging of human iPSCs in 2D and 3D culture, with pluripotency marker expression preserved\u003c\/li\u003e\n\u003cli\u003eSensitive cell types such as hESCs, primary cell isolation, organoid and 3D culture models, cell banking and differentiation workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eReleased comparative data cover CHO-K1, HEK 293, MDCK, Vero and human iPSCs (2D, 3D and differentiated 3D aggregates).\u003c\/strong\u003e Performance on other cell types — hESC, primary cells, organoids, MSCs and others — is stated by the manufacturer as an intended use but is not backed by published figures. As with any change of raw material, qualify the enzyme on your own cells before adopting it in a routine or regulated workflow. A free 100 mL sample is available for exactly that purpose.\u003c\/p\u003e\n\n\u003ch2\u003eInactivation and handling\u003c\/h2\u003e\n\u003cp\u003eCellTrypase requires no special inhibitor. After the cells detach, quench the reaction by adding fresh medium, serum-free or serum-containing. For complete enzyme removal, centrifuge and resuspend the cells in fresh medium. Residual enzyme can be quantified with commercially available ELISA kits for recombinant fungal trypsin-like enzymes; Kerry has experimentally confirmed compatibility with these assay formats.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQuick method for adherent cells:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePre-warm the enzyme and medium to room temperature or 37 °C — warmer temperatures accelerate detachment\u003c\/li\u003e\n\u003cli\u003eRemove spent medium and rinse the monolayer with Ca²⁺\/Mg²⁺-free PBS\u003c\/li\u003e\n\u003cli\u003eAdd approximately 1 mL of the 1× solution per 25 cm²\u003c\/li\u003e\n\u003cli\u003eIncubate until the cells detach — check the monolayer rather than relying on a fixed time; tap the vessel or pipette gently if needed\u003c\/li\u003e\n\u003cli\u003eQuench with fresh medium; centrifuge if the enzyme must be removed\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe 10× concentrate may be used undiluted for fast detachment of strongly adherent cells or diluted to 1× in PBS containing 1 mM EDTA under sterile conditions. The enzyme is most active between pH 7.0 and 10.0 and between 20 and 37 °C; activity falls sharply above 60 °C and is minimal below pH 6.\u003c\/p\u003e\n\n\u003ch2\u003eStorage and shipping\u003c\/h2\u003e\n\u003cp\u003eStore at 2–8 °C, protected from light. Kerry's Product Information Sheet v4.0 (August 2026) confirms stability for at least 21 months from the date of manufacture at 2–8 °C for both the GMP and R\u0026amp;D grades, based on real-time stability data; long-term stability is still under investigation and the shelf life is extended as data accumulate. Temporary exposure to room temperature or above during shipment does not affect enzyme activity, so the product ships at ambient temperature and no cold chain is required. Avoid extended storage at or near 37 °C, which causes a gradual loss of activity through autoproteolysis.\u003c\/p\u003e\n\n\u003ch2\u003eRegulatory support and documentation\u003c\/h2\u003e\n\u003cp\u003eKerry provides documentation to support customer qualification, validation and regulatory submissions, including Certificate of Analysis, Safety Data Sheet, animal origin-free declarations, TSE\/BSE statements, packaging details, change notifications and quality agreements. Both grades are manufactured without antibiotics, animal-derived raw materials or TSE\/BSE-risk materials; the production host is a GMO of safety level S1 and the final product contains no GMO. The Product Information Sheet v4.0 and the four v4.0 product specifications are available below. The CellTrypase Validation Guide v1.2 (release test methods, stability and regulatory statements) and the Application Note v1.1 (full performance data) are supplied on request through the \u003ca href=\"\/pages\/celltrypase-technical-documents\"\u003etechnical documents page\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003csmall\u003e\u003csup\u003e1\u003c\/sup\u003e Calculated using Clone Manager 12 Professional Edition (Sci Ed Software); reflects calculation according to Lehninger. Unit definition: one unit (U) forms 1 µmol \u003cem\u003ep\u003c\/em\u003e-nitroaniline per minute at 37 °C from 8 mM L-AAPA at pH 8.0.\u003c\/small\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eFor Research Use Only. GMP-grade material is intended as a processing aid in biopharmaceutical manufacturing and is not for direct administration to humans or animals. Manufactured and distributed by c-LEcta GmbH, Kerry Biotechnology Centre, Leipzig, Germany — Kerry (formerly c-LEcta). CellTrypase is a trademark of Kerry Group. Free 100 mL samples are available for qualification and process-development testing.\u003c\/em\u003e\u003c\/p\u003e","brand":"Kerry (formerly c-LEcta)","offers":[{"title":"R\u0026D Grade \/ 1x (Ready-to-Use) \/ 100 mL","offer_id":53379624829293,"sku":"22103-1X-100","price":32.0,"currency_code":"USD","in_stock":true},{"title":"R\u0026D Grade \/ 1x (Ready-to-Use) \/ 500 mL","offer_id":53379625091437,"sku":"22103-1X-500","price":110.0,"currency_code":"USD","in_stock":true},{"title":"R\u0026D Grade \/ 10x (Concentrated Stock) \/ 100 mL","offer_id":53379625124205,"sku":"22103-10X-100","price":104.0,"currency_code":"USD","in_stock":true},{"title":"R\u0026D Grade \/ 10x (Concentrated Stock) \/ 500 mL","offer_id":53379625156973,"sku":"22103-10X-500","price":451.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 1x (Ready-to-Use) \/ 100 mL","offer_id":53379625189741,"sku":"22103-1X-100-G","price":42.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 1x (Ready-to-Use) \/ 500 mL","offer_id":53379625222509,"sku":"22103-1X-500-G","price":146.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 10x (Concentrated Stock) \/ 100 mL","offer_id":53379625255277,"sku":"22103-10X-100-G","price":138.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 10x (Concentrated Stock) \/ 500 mL","offer_id":53379625288045,"sku":"22103-10X-500-G","price":602.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CellTrypase_Products_20Packaging.jpg?v=1779996972"}],"url":"https:\/\/www.ebiohippo.com\/collections\/kerry.oembed","provider":"BioHippo","version":"1.0","type":"link"}