{"title":"Lentiviral Vectors","description":"","products":[{"product_id":"b7-1-cd80-orf-cdna-lentivirus-bhv19400112","title":"B7-1 (CD80) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD80, also known as B7-1, is a costimulatory cell-surface glycoprotein of the immunoglobulin superfamily expressed mainly on antigen-presenting cells such as dendritic cells, activated B cells, and macrophages. By binding the receptors CD28 and CTLA-4 on T cells, CD80 delivers a critical second signal that, together with T cell receptor engagement, governs T cell activation, proliferation, and tolerance. Engagement of CD28 promotes T cell activation, whereas binding to CTLA-4 transmits an inhibitory signal that restrains immune responses. Because the B7-CD28\/CTLA-4 axis is central to immune regulation and tumor immune evasion, CD80 is widely studied in immunology, cancer immunotherapy, and checkpoint research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe B7-1 (CD80) ORF cDNA Lentivirus enables stable overexpression of the human or mouse CD80 open reading frame (NM_005191) in mammalian cells. A CMV or EF1a promoter drives the CD80 ORF fused to a C-terminal V5 epitope tag, with a fluorescent reporter (GFP or RFP) and an antibiotic selection marker separated by a self-cleaving peptide for independent translation from a single transcript. The optimized third-generation lentiviral vector provides high gene expression and reliable genome integration, allowing rapid establishment of stable cell lines. Applications include studying CD80 costimulatory function, T cell activation, immune checkpoint biology, and tumor immunology. Particles transduce difficult-to-transfect cells, including primary and thawed cells; cloning accuracy is confirmed by sequencing and protein expression validated by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of B7-1\/CD80 (NCBI Accession: NM_005191, NM_009855) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-B7-1-V5 \/ GFP\/none \/ 5x10^6","offer_id":53251615293805,"sku":"LCV-0020-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd80-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311261507949,"sku":"LCV-0020-6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-1-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311261540717,"sku":"LCV-0020-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd80-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311261573485,"sku":"LCV-0020-5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-1-Flag \/ none\/Puromycin \/ 5x10^6","offer_id":53311261606253,"sku":"LCV-0020-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-1-Flag \/ none\/Blasticidin \/ 5x10^6","offer_id":53311261639021,"sku":"LCV-0020-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cell-cycle-reporter-lentivirus-bhv19400048","title":"Cell Cycle Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCell cycle progression through the G1, S, G2, and M phases is tightly coordinated to ensure faithful DNA replication and division. A key mechanism of phase control is regulated protein degradation: the licensing factor CDT1 accumulates in G1 and is degraded as cells enter S phase, while Geminin accumulates during S, G2, and M and is degraded at the end of mitosis. The fluorescent ubiquitination-based cell cycle indicator (FUCCI) exploits these reciprocal oscillations by fusing fluorescent proteins to fragments of CDT1 and Geminin, allowing real-time visualization of cell cycle phase in living cells. This approach is widely used in studies of proliferation, differentiation, stem cell biology, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Cell Cycle Reporter Lentivirus delivers a FUCCI-based system for visualizing cell cycle progression in living cells without fixation. Human CDT1 (amino acids 30-120), which accumulates in G1, is fused to RFP, and human Geminin (amino acids 1-110), which accumulates in G2\/M, is fused to GFP. A CMV promoter drives RFP-CDT1, GFP-Geminin, and a drug selection marker as a single transcript, with self-cleaving T2A and P2A sequences yielding three separate proteins for balanced reporter expression.\u003c\/p\u003e\n\u003cp\u003eThis arrangement enables precise monitoring of all cell cycle phases in stable or transiently transduced cells. Supplied as VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product suits a wide range of mammalian cells, including primary, thawed, and pluripotent stem cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, GFP, hCDT1-RFP + hGemn-GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Cell cycle (phases) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615326573,"sku":"LTV-0052-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251635347821,"sku":"LTV-0052-2S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FUCCI-Puro-Fig.1.jpg?v=1776934330"},{"product_id":"human-puma-shrna-lentivirus-bhv19400154","title":"Human PUMA shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePUMA (p53 upregulated modulator of apoptosis), encoded by BBC3, is a BH3-only member of the BCL-2 protein family and a key effector of apoptosis. PUMA expression is induced by p53 following DNA damage and by p53-independent stimuli such as growth factor withdrawal and cellular stress. Once expressed, PUMA binds and inhibits anti-apoptotic BCL-2 proteins, freeing BAX and BAK to permeabilize the mitochondrial outer membrane and trigger caspase activation. Through this role, PUMA links diverse stress signals to programmed cell death and contributes to tumor suppression, making it an important target in cancer research, apoptosis studies, and investigations of treatment resistance.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PUMA shRNA Lentivirus delivers validated short hairpin RNA targeting BBC3 from a third-generation, self-inactivating lentiviral backbone, with shRNA expression driven by a U6 promoter and a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping supports broad tropism, including primary, suspension, and cryopreserved cells. Each shRNA is validated to achieve at least 70% PUMA knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eThe high-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of apoptosis, tumor suppression, and stress responses, and generation of stable knockdown cell lines.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BBC3 (NCBI Accession: NM_014417.5) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP\/Luc, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251615359341,"sku":"LSV-0038-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311254135149,"sku":"LSV-0038-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311254167917,"sku":"LSV-0038-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311254200685,"sku":"LSV-0038-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311254233453,"sku":"LSV-0038-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311254266221,"sku":"LSV-0038-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311254298989,"sku":"LSV-0038-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311254331757,"sku":"LSV-0038-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311254364525,"sku":"LSV-0038-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311254397293,"sku":"LSV-0038-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0038-h-PUMA-sh-fig1.jpg?v=1776934210"},{"product_id":"cd16a-nfat-reporter-lentivirus-adcc-assay-bhv19400252","title":"CD16A\/NFAT Reporter Lentivirus (ADCC Assay)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD16A (FcγRIIIa) is a low-affinity Fc receptor for immunoglobulin G expressed on natural killer cells and other effector cells. By engaging the Fc region of antibodies bound to a target cell, CD16A triggers antibody-dependent cellular cytotoxicity (ADCC), a key mechanism by which therapeutic antibodies eliminate tumor and virally infected cells. Receptor engagement raises intracellular calcium and activates the transcription factor NFAT, providing a measurable transcriptional readout of effector-cell activation. A common polymorphism produces high-affinity (V158) and low-affinity (F158) allotypes that influence the strength of ADCC, making CD16A a central focus of antibody-engineering and immuno-oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CD16A\/NFAT Reporter Lentivirus is an immunotherapy reporter system for quantifying antibody-dependent cellular cytotoxicity. A receptor lentivirus constitutively expresses the human CD16A high-affinity V158 allotype with antibiotic selection, intended for use alongside a companion low-affinity F158 product as a control. A reporter lentivirus carries NFAT response elements driving a dual reporter, secreted Gaussia luciferase and a fluorescent protein. Sequential transduction and selection generates a dual-stable effector cell line, such as Jurkat, that responds to antibody-coated target cells.\u003c\/p\u003e\n\u003cp\u003eWhen CD16A engages target-bound IgG, intracellular calcium rises and activates NFAT, driving the reporters. Secreted Gaussia luciferase allows kinetic sampling of media without lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped, PEG- and sucrose-gradient-purified particles transduce difficult-to-transfect cells, supporting ADCC potency testing and antibody screening.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human ADCC Assay under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CD16A(V158)-GFP\/NFAT-RFP \/ RFP \/ 2x10^6","offer_id":53251615392109,"sku":"TRV-0002-1S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-RFP\/NFAT-GFP \/ GFP \/ 2x10^6","offer_id":53362085593453,"sku":"TRV-0002-2S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-GFP \/ GFP \/ 2x10^6","offer_id":53362085626221,"sku":"TRV-0002-3S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-RFP \/ RFP \/ 2x10^6","offer_id":53362085658989,"sku":"TRV-0002-4S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-GFP-GLuc \/ GFP-P2A-GLuc \/ 2x10^6","offer_id":53362085691757,"sku":"TRV-0002-6S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(F158)-BSD\/NFAT-GFP-GLuc (Low Affinity) \/ GFP-P2A-GLuc \/ 2x10^6","offer_id":53362085724525,"sku":"TRV-0002-6N","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-GFP-GLuc Jurkat Cells \/ GFP-P2A-GLuc \/ 5x10^6 cells","offer_id":53362085757293,"sku":"TRC-0002-6C","price":1695.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ADCC-jurkat-flow.jpg?v=1776934273"},{"product_id":"btla1-nfat-reporter-lentivirus-bhv19400276","title":"BTLA1\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBTLA (B and T lymphocyte attenuator, also called CD272 and referred to here as BTLA1) is a co-inhibitory receptor of the immunoglobulin superfamily expressed on T cells, B cells, and other lymphocytes. Unlike most checkpoint receptors, BTLA binds a TNF-family ligand, HVEM, and the resulting interaction recruits phosphatases through ITIM motifs to attenuate antigen-receptor signaling. By dampening lymphocyte activation, BTLA contributes to peripheral tolerance and limits excessive immune responses, but it can also restrain anti-tumor immunity. Because BTLA engagement suppresses NFAT-dependent transcription downstream of the T cell receptor, NFAT reporter systems provide a quantitative readout of BTLA signaling for checkpoint and immunotherapy research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe BTLA1\/NFAT Reporter Lentivirus is a two-component immunotherapy reporter system. A receptor construct constitutively expresses the human BTLA receptor together with an antibiotic selection marker, while a reporter construct drives a dual reporter through tandem NFAT response elements coupled to a minimal promoter. Reporter options include a fluorescent protein (GFP or RFP) and secreted Gaussia luciferase, allowing readout by both fluorescence and luminometry.\u003c\/p\u003e\n\u003cp\u003eSequential transduction and selection generate a dual-stable effector cell line that responds quantitatively to receptor engagement. Applications include studying BTLA checkpoint signaling, testing checkpoint-blockade agents, and characterizing T cell inhibition. Supplied as VSV-G-pseudotyped lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, suitable for primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human BTLA1 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251615424877,"sku":"TRV-0028-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"hoxb13-reporter-lentivirus-bhv19400059","title":"HOXB13 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHOXB13 (homeobox B13) is a homeodomain transcription factor of the HOX family, regulators of body patterning and tissue identity during development. HOXB13 contributes to the development and homeostasis of posterior structures and hormone-responsive tissues. In the prostate it acts as a context-dependent modulator of androgen receptor signaling, including functions as a repressor of androgen receptor activity, and thereby influences epithelial differentiation and growth regulation. Germline variants in HOXB13 are associated with hereditary prostate cancer risk, and altered HOXB13 expression affects tumor cell behavior. The factor is studied for its roles in developmental patterning, hormone-responsive transcription, and prostate biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HOXB13 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of HOXB13 activity in mammalian cells. The construct contains tandem repeats of the HOXB13 DNA-binding element placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying HOXB13 transcriptional activity in primary and difficult-to-transfect cells for developmental and endocrine research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Androgen Receptor (AR) Pathway (HOXB13) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615457645,"sku":"LTV-0064-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310901944685,"sku":"LTV-0064-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310901977453,"sku":"LTV-0064-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310902010221,"sku":"LTV-0064-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310902042989,"sku":"LTV-0064-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310902075757,"sku":"LTV-0064-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fig1-replace.jpg?v=1776934390"},{"product_id":"geci-reporter-lentivirus-bhv19400050","title":"GECI Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eGenetically encoded calcium indicators (GECIs) are protein-based sensors that convert changes in intracellular Ca2+ concentration into measurable fluorescence. Calcium is a universal second messenger, and its transient elevation underlies neuronal firing, cardiac myocyte contraction, secretion, and many signaling events downstream of receptor activation. GCaMP-family sensors fuse a circularly permuted fluorescent protein to calmodulin and a calmodulin-binding peptide, so that Ca2+ binding produces a rapid, reversible increase in brightness. Driven by neuron-specific promoters such as human synapsin, GECIs allow real-time imaging of activity in defined cell populations. These tools are central to systems neuroscience, cardiac physiology, and studies of calcium dynamics in health and disease.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe GECI Reporter Lentivirus delivers a genetically encoded Ca2+ indicator for real-time imaging of cytosolic calcium in living mammalian cells. Neuron-specific human synapsin promoter versions express the jGCaMP7b or red-shifted jYCaMP1s sensors for recording neuronal calcium signaling in vitro, while CMV-driven versions enable measurement in cardiac myocytes and kidney cells. A drug-selection marker (puromycin or blasticidin) supports rapid establishment of stable reporter cell lines, typically within about ten days. Applications include monitoring neuronal activity, cardiac and renal calcium handling, and potential transduction of brain cortex neurons to interrogate neuronal activity in vivo. Supplied as high-quality pre-packaged lentivirus purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, JGCamP, JYCamP, mCherry, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Calcium signaling transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615490413,"sku":"LTV-0054-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898504045,"sku":"LTV-0054-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898536813,"sku":"LTV-0054-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898569581,"sku":"LTV-0054-4S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_744cb70d-4ea4-4271-8a5c-2c6987a14742.jpg?v=1776934210"},{"product_id":"human-igf1-shrna-lentivirus-bhv19400134","title":"Human IGF1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIGF1 (insulin-like growth factor 1), also known as somatomedin-C, is a peptide hormone structurally related to insulin. Produced largely by the liver under growth hormone control, and also locally in many tissues, IGF1 acts through the IGF1 receptor tyrosine kinase to activate the PI3K-AKT and RAS-MAPK pathways. This signaling promotes cell proliferation, growth, survival, and differentiation, and IGF1 is a central mediator of postnatal somatic growth and tissue regeneration. Dysregulated IGF1 signaling is associated with growth disorders, metabolic disease, and cancer progression, making IGF1 an important target in endocrinology, metabolism, and oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human IGF1 shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human insulin-like growth factor 1 (IGF1). The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of IGF1 signaling in cell growth, proliferation, metabolism, and tumor biology. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting IGF1 (NCBI Accession: NM_001111283) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251615523181,"sku":"LSV-0019-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311254430061,"sku":"LSV-0019-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311254462829,"sku":"LSV-0019-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311254495597,"sku":"LSV-0019-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311254528365,"sku":"LSV-0019-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311254561133,"sku":"LSV-0019-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311254593901,"sku":"LSV-0019-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311254626669,"sku":"LSV-0019-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311254659437,"sku":"LSV-0019-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311254692205,"sku":"LSV-0019-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0019-h-IGF1-sh-Fig1.jpg?v=1776934142"},{"product_id":"clec4e-nf-kappa-b-reporter-lentivirus-bhv19400284","title":"CLEC4E\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCLEC4E, also known as Mincle, is a C-type lectin receptor expressed mainly on myeloid cells such as macrophages and dendritic cells. It functions as a pattern-recognition receptor that senses pathogen-associated molecular patterns, including mycobacterial glycolipids, as well as damage-associated ligands released from dying cells. Ligand binding triggers signaling through the FcRgamma adaptor and the Syk-CARD9 axis, leading to activation of the NF-κB family of transcription factors and the production of pro-inflammatory cytokines. Through this pathway, CLEC4E shapes innate immune responses to infection and tissue damage, making it an important focus in research on antifungal and antimycobacterial immunity and inflammation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CLEC4E\/NF-κB Reporter Lentivirus is an all-in-one immunotherapy reporter system for studying CLEC4E (Mincle) signaling in immune cells. The construct constitutively expresses the human CLEC4E receptor and couples ligand recognition to NF-κB-dependent transcription of dual reporters: secreted Gaussia luciferase and a fluorescent protein (GFP or RFP), enabling real-time monitoring by luminometry and fluorescence.\u003c\/p\u003e\n\u003cp\u003eTransducing target cells with the high-titer lentivirus establishes a stable, pathway-specific reporter cell line. Applications include studying innate immunity, characterizing pathogen-sensing mechanisms, and evaluating CLEC4E-targeted compounds. Supplied as third-generation, VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product is effective in difficult-to-transfect primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CLEC4E under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251615555949,"sku":"TRV-0036-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"anre-reporter-lentivirus-bhv19400041","title":"AnRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe androgen response element (AnRE) is a DNA sequence that mediates transcription driven by the androgen receptor (AR), a ligand-activated nuclear receptor. Upon binding androgens such as testosterone or dihydrotestosterone, AR translocates to the nucleus, dimerizes, and binds androgen response elements to regulate genes controlling male sexual differentiation, muscle and bone physiology, and prostate function. AR signaling is a central driver of prostate cancer, where it remains a key therapeutic target, and is also studied in endocrinology and steroid hormone biology. AnRE-driven reporters give a quantitative readout of androgen receptor transcriptional activity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AnRE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem androgen response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of androgen receptor activation. Reporter options include EGFP, GFP, mCherry, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring AR signaling pathway activity, characterizing androgens and anti-androgens, and screening compounds that modulate androgen receptor function in endocrinology and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Androgen receptor (AR) signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615654253,"sku":"LTV-0045-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898307437,"sku":"LTV-0045-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898340205,"sku":"LTV-0045-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898372973,"sku":"LTV-0045-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310898405741,"sku":"LTV-0045-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310898438509,"sku":"LTV-0045-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898471277,"sku":"LTV-0045-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_8ea109d9-4f73-48d4-a10e-d6a4fd0aef7f.jpg?v=1776934340"},{"product_id":"p53-reporter-lentivirus-bhv19400007","title":"P53 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTP53 encodes p53, a tumor suppressor transcription factor often described as the guardian of the genome. In response to cellular stresses such as DNA damage, oncogene activation, and hypoxia, p53 is stabilized and binds specific response elements to activate genes that drive cell cycle arrest, DNA repair, senescence, and apoptosis. Through this program, p53 prevents the propagation of damaged or potentially malignant cells. TP53 is the most frequently mutated gene in human cancer, and loss of p53 function compromises these protective responses. Because of its central role, p53 transcriptional activity is widely monitored in studies of genotoxic stress, apoptosis, and tumor suppression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe P53 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of p53 activity in human or mouse cells. Tandem consensus p53 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, or Renilla luciferase), so that p53 activation produces a quantitative signal indicative of p53 and apoptosis pathway engagement in transduced cells. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study genotoxic stress responses, apoptosis, and tumor suppressor signaling, and to screen modulators of p53 activity. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Apoptosis\/p53 signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615621485,"sku":"LTV-0008-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893982061,"sku":"LTV-0008-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310894014829,"sku":"LTV-0008-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894047597,"sku":"LTV-0008-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310894080365,"sku":"LTV-0008-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894113133,"sku":"LTV-0008-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_b476d082-2641-436d-80a2-b5c8f467b270.jpg?v=1776934151"},{"product_id":"bre-reporter-lentivirus-bhv19400070","title":"BRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBone morphogenetic proteins (BMPs) are members of the TGF-β superfamily that signal through type I and type II serine\/threonine kinase receptors. Ligand binding activates the receptor-regulated SMADs (SMAD1, SMAD5, and SMAD8), which form a complex with the common mediator SMAD4 and accumulate in the nucleus, where they bind BMP response elements (BRE) to regulate target gene transcription. BMP signaling controls embryogenesis, skeletal and organ development, stem cell fate, and adult tissue homeostasis. Dysregulated BMP signaling is associated with skeletal disorders, fibrosis, and cancer, making BRE-driven reporters useful tools for studying this pathway.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe BRE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of BMP signaling pathway activity in mammalian cells. The construct contains inverted repeats of BMP response elements from the Id2 promoter plus a SMAD4 DNA-binding motif; upon BMP pathway activation, the SMAD1\/5\/8-SMAD4 complex binds the BRE and drives reporter expression. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, luminometry, or β-galactosidase assay. The system is applied to study BMP signaling in development, stem cell differentiation, and tissue homeostasis. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP, β-Galactosidase reporter gene under the control of tandem consensus response elements specific for the BMP Signaling Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615719789,"sku":"LTV-0075-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310904566125,"sku":"LTV-0075-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310904598893,"sku":"LTV-0075-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310904631661,"sku":"LTV-0075-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310904664429,"sku":"LTV-0075-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310904697197,"sku":"LTV-0075-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/BRE-Reporter-Flow-Data.jpg?v=1776934145"},{"product_id":"gal4-dbd-ar-lbd-bsd-bhv19400188","title":"GAL4-DBD-AR-LBD-BSD","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe androgen receptor (AR), encoded by the NR3C4 gene, is a steroid hormone nuclear receptor that mediates the physiological actions of androgens such as testosterone and dihydrotestosterone. It is essential for the development and maintenance of reproductive tissues, muscle, and bone, and contributes to various metabolic processes. AR is highly expressed in androgen-responsive tissues, where it regulates genes that drive male characteristics and muscle growth. Upon binding hormone ligand, AR dissociates from accessory chaperones, translocates to the nucleus, and activates transcription of androgen-responsive genes. Because of its roles in endocrine physiology and cancer, AR is a major focus in oncology and endocrinology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eGAL4-DBD-AR-LBD-BSD is a GAL4-nuclear receptor reporter lentivirus that expresses a chimeric protein joining the yeast GAL4 DNA-binding domain to the ligand-binding domain (LBD) of the human androgen receptor. Upon ligand binding, the LBD recruits co-activators and the chimera binds upstream GAL4 response elements to drive a fluorescent or luminescent reporter (GFP, RFP, or firefly luciferase), with high signal-to-noise relative to endogenous nuclear receptor background.\u003c\/p\u003e\n\u003cp\u003eThe GAL4-LBD design isolates LBD-mediated transactivation and prevents cross-reactivity with endogenous receptor targets, enabling specific pharmacological characterization of AR agonists, antagonists, and selective androgen receptor modulators. Supplied as high-titer lentiviral particles, it allows generation of stable cell lines for ligand profiling and is amenable to high-throughput screening by luminometry.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentiviral system expresses a chimeric protein consisting of the GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of \u003cstrong\u003eAR\u003c\/strong\u003e. Upon specific ligand binding, the LBD undergoes conformational change and recruits co-activators; the activated chimera binds upstream GAL4 response elements (UAS×5) to drive expression of a fluorescent or luminescent reporter (GFP, RFP, or Firefly Luciferase) with high signal-to-noise relative to endogenous nuclear receptor background.\u003c\/p\u003e\n\u003cp\u003eThe GAL4-LBD design isolates LBD-mediated transactivation from A\/B domain-driven constitutive activity and prevents cross-reactivity with endogenous nuclear receptor targets, enabling highly specific pharmacological characterization of LBD agonists, antagonists, and selective receptor modulators (SARMs\/SERMs\/SPPARMs). This system is established for drug discovery in academic and industrial settings, and is amenable to high-throughput screening (HTS) formats using luminometry.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GAL4-DBD-AR-LBD-BSD \/ Blasticidin \/ 3x10^6","offer_id":53251615687021,"sku":"GNV-0004-1S","price":995.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAG-Puro \/ Puromycin \/ 5x10^6","offer_id":53362027594093,"sku":"GNV-0004-1G","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAR-Puro \/ Puromycin \/ 5x10^6","offer_id":53362027626861,"sku":"GNV-0004-6G","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAL-Puro \/ Puromycin \/ 2x10^6","offer_id":53362027659629,"sku":"GNV-0004-3G","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GAL4-Graphic-scaled.jpg?v=1776934280"},{"product_id":"ap2-reporter-lentivirus-bhv19400250","title":"AP2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AP-2 family of transcription factors, encoded by genes including TFAP2A, TFAP2B, and TFAP2C, are key regulators of development. They are strongly expressed in neural crest cells and their derivatives, where they control facial and craniofacial development, limb patterning, and organogenesis. AP-2 factors bind GC-rich DNA elements and influence cell proliferation, differentiation, and apoptosis across multiple tissues. Loss or dysregulation of AP-2 activity is associated with developmental disorders, while altered AP-2 function also contributes to tumorigenesis in several cancers. Because of these roles, AP-2-dependent transcription is an informative readout in developmental biology and cell signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AP2 Reporter Lentivirus is a transcription-factor reporter system that detects transcriptional activity mediated by AP-2 (TFAP2) factors in human and mouse cells. The construct uses tandem repeats of AP-2 DNA-binding elements derived from the human metallothionein IIa promoter coupled to a minimal promoter to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, and luciferase configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying neural crest development, AP-2 regulation of differentiation, and AP-2 dysregulation in disease. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the AP-2 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"AP-2-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251615785325,"sku":"LTV-0143-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362091884909,"sku":"LTV-0143-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362091917677,"sku":"LTV-0143-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362091950445,"sku":"LTV-0143-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362091983213,"sku":"LTV-0143-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362092015981,"sku":"LTV-0143-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362092048749,"sku":"LTV-0143-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362092081517,"sku":"LTV-0143-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362092114285,"sku":"LTV-0143-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362092147053,"sku":"LTV-0143-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362092179821,"sku":"LTV-0143-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AP2-TAG-PURO-THUMB.jpg?v=1776934133"},{"product_id":"acvr1c-orf-cdna-lentivirus-bhv19400118","title":"ACVR1C ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eACVR1C, also known as ALK7, is a type I serine\/threonine kinase receptor of the TGF-beta superfamily. Together with type II receptors, it binds ligands such as Nodal, Activin B, and GDF3, leading to phosphorylation of SMAD2 and SMAD3 and activation of downstream transcriptional responses. Through this pathway, ACVR1C regulates cell growth, differentiation, apoptosis, and metabolic processes, with notable roles in adipose tissue function, insulin sensitivity, and pancreatic and reproductive biology. Because TGF-beta superfamily signaling influences both normal development and disease, including metabolic disorders and cancer, ACVR1C is a useful target for studies of receptor-mediated SMAD signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ACVR1C ORF cDNA Lentivirus delivers lentiviral particles packaged with an expression vector for human ACVR1C. A CMV or EF1a promoter drives the ORF cDNA fused to a C-terminal epitope tag, while a fluorescent reporter (GFP, RFP, or BFP) and drug-selection marker (puromycin or blasticidin) are separated by a self-cleaving peptide for independent expression from the same transcript; in some constructs the reporter and marker are driven separately by a PGK promoter. ORF cloning accuracy has been confirmed by sequencing and protein expression validated by transient transfection. The vectors provide high, stable ACVR1C expression and reliable genome integration, enabling long-term stable cell lines. They efficiently transduce difficult-to-transfect cells, including primary and thawed cells, for studies of TGF-beta superfamily and SMAD signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of ACVR1C (NCBI Accession: NM_145259) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (BFP, GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-ACVR1C-BFP-BSD \/ BFP\/Blasticidin \/ 2x10^6","offer_id":53251615752557,"sku":"LCV-0026-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-ACVR1C-GFP-Puro \/ GFP\/Puromycin \/ 2x10^6","offer_id":53251634790765,"sku":"LCV-0026-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-ACVR1C-RFP-BSD \/ RFP\/Blasticidin \/ 2x10^6","offer_id":53251634823533,"sku":"LCV-0026-3S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-bid-shrna-lentivirus-bhv19400147","title":"Human BID shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBID (BH3 interacting domain death agonist) is a BH3-only member of the BCL-2 protein family that links the extrinsic and intrinsic apoptotic pathways. Following death receptor activation, caspase-8 cleaves BID to generate truncated BID (tBID), which translocates to mitochondria and activates the effectors BAX and BAK. This triggers mitochondrial outer membrane permeabilization, release of cytochrome c, and downstream caspase activation, amplifying the apoptotic signal. By coupling death receptor signaling to the mitochondrial pathway, BID is essential for efficient apoptosis in many cell types. Its regulation influences sensitivity to cell death, and dysregulation contributes to tumorigenesis and therapy resistance.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human BID shRNA Lentivirus delivers validated short hairpin RNA targeting BID from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% BID knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of death receptor signaling, mitochondrial apoptosis, and apoptotic crosstalk.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BID (NCBI Accession: NM_197966.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251615818093,"sku":"LSV-0032-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311253545325,"sku":"LSV-0032-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311253578093,"sku":"LSV-0032-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311253610861,"sku":"LSV-0032-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311253643629,"sku":"LSV-0032-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311253676397,"sku":"LSV-0032-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311253709165,"sku":"LSV-0032-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311253741933,"sku":"LSV-0032-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311253774701,"sku":"LSV-0032-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311253807469,"sku":"LSV-0032-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0032-h-BID-sh-fig1-1.jpg?v=1776934218"},{"product_id":"atf6-reporter-lentivirus-bhv19400092","title":"ATF6 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eActivating transcription factor 6 (ATF6) is an ER membrane-bound bZIP transcription factor and one of the three main sensors of the unfolded protein response. When unfolded proteins accumulate in the endoplasmic reticulum, ATF6 traffics to the Golgi, where it is cleaved to release an active nuclear fragment. This fragment binds ER stress-response elements to induce ER chaperones and folding enzymes such as GRP78, as well as transcription factors including XBP1. The ATF6 DNA-binding sequence is related to but distinct from ATF1\/CREB elements. ATF6 signaling is essential for adapting to ER stress and is implicated in cancer, metabolic disease, and protein-misfolding disorders.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ATF6 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of consensus ATF6 DNA-binding elements upstream of a minimal promoter and a reporter gene, giving a sensitive fluorescent or luminescent readout of ATF6 transcriptional activity. The element responds specifically to active ATF6 and is strongly induced by ER stress stimuli such as tunicamycin. A wide range of reporters is available, including EGFP, mCherry, BFP2, d2GFP, firefly luciferase, Gaussia luciferase, and Renilla luciferase, with selection markers including blasticidin, hygromycin, puromycin, and zeocin. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying ER stress and the unfolded protein response.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the ATF6 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615850861,"sku":"LTV-0101-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251634037101,"sku":"LTV-0101-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53251634069869,"sku":"LTV-0101-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53251634102637,"sku":"LTV-0101-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53251634135405,"sku":"LTV-0101-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53251634168173,"sku":"LTV-0101-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Puromycin \/ 5x10^6","offer_id":53251634200941,"sku":"LTV-0101-15S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Blasticidin \/ 5x10^6","offer_id":53251634233709,"sku":"LTV-0101-16S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-general-2_aab39aa6-5d35-4e8d-ad95-dba95b136483.jpg?v=1776934516"},{"product_id":"4-1bb-nfat-reporter-lentivirus-bhv19400278","title":"4-1BB\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e4-1BB (CD137, TNFRSF9) is a co-stimulatory receptor of the tumor necrosis factor receptor superfamily, induced on activated T cells and natural killer cells. Engagement by its ligand 4-1BBL delivers a powerful co-stimulatory signal that enhances T cell activation, proliferation, survival, and the formation of memory populations. 4-1BB signaling proceeds through TRAF adaptors to activate downstream pathways, and amplifies antigen-receptor-driven NFAT-dependent transcription. Because of its ability to boost effector responses, the 4-1BB axis is a major target for agonist immunotherapies and is incorporated as a co-stimulatory domain in chimeric antigen receptor designs. NFAT reporters provide a quantitative readout of 4-1BB-enhanced T cell activation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe 4-1BB\/NFAT Reporter Lentivirus is an all-in-one immunotherapy reporter system for studying 4-1BB signaling in T cells. The construct constitutively expresses the human 4-1BB receptor and couples its engagement to NFAT-dependent transcription of dual reporters: secreted Gaussia luciferase and a fluorescent protein (GFP or RFP), enabling real-time monitoring of NFAT activation by luminometry and fluorescence.\u003c\/p\u003e\n\u003cp\u003eTransducing target cells with the high-titer lentivirus establishes a stable, pathway-specific reporter cell line. Applications include evaluating co-stimulatory signaling, testing agonistic antibodies, and studying immune activation pathways. Supplied as third-generation, VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product performs well in difficult-to-transfect primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human 4-1BB under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251615883629,"sku":"TRV-0030-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"c-ebp-alpha-reporter-lentivirus-bhv19400054","title":"C\/EBP-α Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eC\/EBP-α (CEBPA) is a basic leucine zipper transcription factor and a key regulator of cell differentiation and metabolism. By binding CCAAT-box sequences in target gene promoters, it controls the differentiation of adipocytes, hepatocytes, and granulocytes, and coordinates genes involved in lipid and glucose metabolism and energy balance. C\/EBP-α also restrains cell proliferation, helping couple differentiation to cell-cycle exit. In adipocyte and hepatic biology, C\/EBP-α acts together with PPAR pathway factors to drive metabolic gene programs. Loss-of-function mutations in CEBPA are recurrent in acute myeloid leukemia, making it a target of interest in metabolism, differentiation, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe C\/EBP-α Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of C\/EBP-α transcriptional activity in human or mouse cells. Reporter expression is controlled by C\/EBP-responsive elements, so signal intensity reflects endogenous C\/EBP-α pathway activation. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study C\/EBP-α function in adipocyte and hepatocyte differentiation, metabolic gene regulation, and myeloid biology. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the C\/EBP-a, PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615916397,"sku":"LTV-0058-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310903419245,"sku":"LTV-0058-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310903452013,"sku":"LTV-0058-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310903484781,"sku":"LTV-0058-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310903517549,"sku":"LTV-0058-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310903550317,"sku":"LTV-0058-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0058-CEBPA-reporter-Fig1.jpg?v=1776934139"},{"product_id":"h-m-bcl-xl-shrna-lentivirus-bhv19400142","title":"h\/m BCL-xL shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBCL-xL, encoded by BCL2L1, is an anti-apoptotic member of the BCL-2 protein family. It promotes cell survival by binding and sequestering pro-apoptotic effectors such as BAX and BAK at the mitochondrial outer membrane, thereby preventing mitochondrial outer membrane permeabilization and the release of cytochrome c. By setting the threshold for intrinsic apoptosis, BCL-xL helps balance cell survival and death during development and tissue homeostasis. BCL-xL is frequently overexpressed in cancers, where it supports tumor cell survival and resistance to chemotherapy, making it an important target in apoptosis and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m BCL-xL shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse BCL2L1 for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of apoptosis and tumor cell survival.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BCL2L1 (NCBI Accession: NM_138578.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251615949165,"sku":"LSV-0027-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311261671789,"sku":"LSV-0027-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311261704557,"sku":"LSV-0027-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311261737325,"sku":"LSV-0027-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311261770093,"sku":"LSV-0027-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311261802861,"sku":"LSV-0027-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311261835629,"sku":"LSV-0027-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311261868397,"sku":"LSV-0027-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311261901165,"sku":"LSV-0027-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311261933933,"sku":"LSV-0027-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0027-h-BCL-xL-sh-fig1.jpg?v=1776934149"},{"product_id":"care-reporter-lentivirus-bhv19400222","title":"CARE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCAR (constitutive androstane receptor, NR1I3) is a ligand-activated transcription factor of the nuclear receptor superfamily and a master regulator of xenobiotic and drug metabolism. CAR heterodimerizes with the retinoid X receptor alpha (RXRα) and binds CAR response elements (CARE), often arranged as direct repeats, in the promoters of cytochrome P450 and other detoxification genes. CAR can be activated through both direct ligand binding and ligand-independent mechanisms, and the CAR-RXRα heterodimer participates in drug metabolism, energy homeostasis, and the modulation of cancer development by regulating numerous target genes, predominantly in hepatocytes. These functions make CARE-driven transcription an informative readout in metabolism and pharmacology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CARE Reporter Lentivirus is a transcription-factor reporter system engineered to detect the transcriptional activity of the constitutive androstane receptor in human and mouse cells. The construct uses tandem repeats of CAR response elements derived from the cytochrome P450 (CYP2B10) gene promoter, arranged in a DR4 pattern to read out the synergistic activity of the CAR\/RXRα heterodimer characteristic of hepatocytes. A fluorescent or luminescent reporter is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying CAR-mediated drug metabolism, energy homeostasis, and CAR pharmacology. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the CAR1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616014701,"sku":"LTV-0113-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362028282221,"sku":"LTV-0113-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362028314989,"sku":"LTV-0113-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362028347757,"sku":"LTV-0113-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362028380525,"sku":"LTV-0113-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362028413293,"sku":"LTV-0113-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CARE-TAG-PURO-FIG1.jpg?v=1776934133"},{"product_id":"aare-reporter-lentivirus-bhv19400033","title":"AARE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe amino acid response element (AARE) mediates transcription during the amino acid response, a branch of the integrated stress response that adapts cells to nutrient limitation. When amino acids are scarce, uncharged tRNAs activate the kinase GCN2, which phosphorylates the translation factor eIF2α. This selectively enhances translation of the transcription factor ATF4, which binds AARE sequences to induce genes for amino acid biosynthesis, transport, and metabolic adaptation. Prolonged amino acid or glucose deprivation can also engage ER stress signaling through the AARE. This nutrient-sensing pathway is central to cellular responses to metabolic stress and is relevant to cancer metabolism and drug development.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AARE Reporter Lentivirus is a transcription factor reporter system that uses tandem repeats of the amino acid response element from the human asparagine synthase promoter, containing the nutrient-sensing response elements NSRE-1 and NSRE-2, to drive a reporter gene. This gives a sensitive fluorescent or luminescent readout of the eIF2α\/ATF4 amino acid response pathway following amino acid or glucose deprivation. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying metabolic stress and integrated stress response signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Amino acid response (eIF2a\/ATF4) pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616047469,"sku":"LTV-0037-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310902501741,"sku":"LTV-0037-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310902534509,"sku":"LTV-0037-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310902567277,"sku":"LTV-0037-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310902600045,"sku":"LTV-0037-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310902632813,"sku":"LTV-0037-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310902665581,"sku":"LTV-0037-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_234b25e7-0049-4d1e-81d7-435ed15f8fb2.jpg?v=1776934331"},{"product_id":"dectin1-nf-kappa-b-reporter-lentivirus-bhv19400266","title":"DECTIN1\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eDectin-1 is a C-type lectin receptor expressed on dendritic cells, macrophages, neutrophils, and other myeloid cells, where it serves as a key pattern recognition receptor of the innate immune system. It recognizes beta-glucans, carbohydrate components of fungal cell walls, and signals through its immunoreceptor tyrosine-based activation-like motif and the Syk kinase pathway. This signaling activates the transcription factor NF-κB, driving the production of pro-inflammatory cytokines, reactive oxygen species, and other effectors of antifungal defense, and helping to shape adaptive immunity, including Th17 responses. Dectin-1 is studied for its central role in antifungal immunity and inflammation and as a target for immunomodulatory and vaccine adjuvant strategies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe DECTIN1\/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human Dectin-1 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable reporter cell line that responds quantitatively to Dectin-1 engagement, for example by fungal beta-glucans, with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media for kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antifungal immunity, inflammatory signaling, and Dectin-1-targeting therapeutics in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human DECTIN1 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616080237,"sku":"TRV-0018-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"ctla4-nfat-reporter-lentivirus-bhv19400277","title":"CTLA4\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCTLA-4 (CD152) is a co-inhibitory receptor and a central regulator of immune checkpoint control. Expressed on activated T cells and constitutively on regulatory T cells, it competes with the co-stimulatory receptor CD28 for the shared B7 ligands CD80 and CD86 on antigen-presenting cells, and binds them with higher affinity. By outcompeting CD28 and removing B7 ligands from the cell surface, CTLA-4 dampens T cell activation, reduces calcium-driven NFAT signaling, and enforces peripheral tolerance. Because CTLA-4 restrains anti-tumor immunity, blocking antibodies are a foundational cancer immunotherapy, and NFAT reporters provide a quantitative readout of CTLA-4-mediated suppression and its reversal.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CTLA4\/NFAT Reporter Lentivirus is an all-in-one immunotherapy reporter system for studying CTLA-4-mediated checkpoint signaling in T cells. The construct constitutively expresses the human CTLA-4 receptor; engagement of B7 ligands suppresses T cell activity, lowering cytosolic calcium, reducing NFAT activation, and decreasing dual reporter output of secreted Gaussia luciferase and a fluorescent protein (GFP or RFP). Anti-CTLA-4 antibodies that block receptor engagement restore reporter activity.\u003c\/p\u003e\n\u003cp\u003eTransducing target cells with the high-titer lentivirus establishes a stable, pathway-specific reporter cell line. Applications include studying immune checkpoint suppression and testing checkpoint-blockade agents. Supplied as third-generation, VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, effective in primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CTLA4 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616113005,"sku":"TRV-0029-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-bik-shrna-lentivirus-bhv19400140","title":"Human BIK shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBIK (BCL2 interacting killer) is a pro-apoptotic member of the BH3-only subgroup of the BCL-2 protein family. By engaging and neutralizing anti-apoptotic proteins such as BCL-2 and BCL-XL, BIK promotes the activation of the effector proteins BAX and BAK, leading to mitochondrial outer membrane permeabilization and intrinsic-pathway apoptosis. BIK localizes largely to the endoplasmic reticulum and contributes to cellular responses to stress and to the regulation of cell survival. Its expression is frequently reduced or silenced in several tumor types, which can blunt apoptotic signaling and support tumor cell survival. BIK is therefore studied as a tumor suppressor and as a potential modulator of treatment sensitivity in cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human BIK shRNA Lentivirus delivers a validated short hairpin RNA targeting human BIK for stable, RNA interference-based silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and an antibiotic selection marker (Puromycin or Blasticidin) co-expressed for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown efficiency is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation. The product supports loss-of-function studies of BIK in apoptosis regulation, tumor suppression, and cancer cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BIK (NCBI Accession: NM_001197.5) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616145773,"sku":"LSV-0025-1S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0025-h-BIK-sh-fig1.jpg?v=1776934220"},{"product_id":"fxre-er2-reporter-lentivirus-bhv19400237","title":"FXRE\/ER2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe farnesoid X receptor (FXR) is a bile acid-activated nuclear receptor that functions as a master regulator of metabolic homeostasis. Activated FXR heterodimerizes with the retinoid X receptor and binds FXR response elements (FXRE) to regulate genes controlling bile acid synthesis and transport, lipid and glucose metabolism, and amino acid handling. The FXRE arranged as an everted repeat separated by two nucleotides (ER2) preferentially responds to the FXR-alpha2 and FXR-alpha4 isoforms, allowing isoform-selective monitoring of FXR activity. FXR signaling protects against bile acid toxicity and helps maintain liver and intestinal health, and its dysregulation is linked to cholestasis, metabolic disease, and liver disorders, making it an important target in metabolism and signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FXRE\/ER2 Reporter Lentivirus is a transcription factor reporter system that detects transcriptional activity mediated by the farnesoid X receptor, with preferential readout of the FXR-alpha2 and FXR-alpha4 isoforms. The construct contains tandem FXR response elements arranged in the everted repeat ER2 configuration, placed upstream of a minimal promoter driving a fluorescent or luminescent reporter, with an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin, Hygromycin, Puromycin, or Zeocin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is suited to primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FXR\/ERa2 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"FXRE\/ER2-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251616178541,"sku":"LTV-0130-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089066861,"sku":"LTV-0130-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089099629,"sku":"LTV-0130-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362089132397,"sku":"LTV-0130-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362089165165,"sku":"LTV-0130-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362089197933,"sku":"LTV-0130-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362089230701,"sku":"LTV-0130-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362089263469,"sku":"LTV-0130-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362089296237,"sku":"LTV-0130-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362089329005,"sku":"LTV-0130-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362089361773,"sku":"LTV-0130-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fxre-er2-1.jpg?v=1776934151"},{"product_id":"c-ebp-beta-reporter-lentivirus-bhv19400029","title":"C\/EBP-β Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eC\/EBP-β (CCAAT\/enhancer-binding protein beta), encoded by CEBPB, is a bZIP transcription factor that binds CCAAT-box DNA elements to regulate gene expression. It is a key regulator of adipocyte differentiation, where it acts early in the transcriptional cascade that activates PPARγ and drives terminal adipogenesis. C\/EBP-β also controls the acute-phase inflammatory response, macrophage function, and metabolic gene expression in liver and other tissues. Through its roles in metabolism, immunity, and cell differentiation, C\/EBP-β is widely studied in metabolic disease, inflammation, and cell signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe C\/EBP-β Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem consensus C\/EBP DNA-binding elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of C\/EBP-β transcriptional activity in transduced cells. Reporter options include GFP, RFP, and luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring C\/EBP pathway activation, studying adipocyte differentiation and inflammatory signaling, and screening compounds that modulate C\/EBP-β activity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a GFP, Luc, RFP reporter gene under the control of tandem consensus response elements specific for the C\/EBP-b, PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616211309,"sku":"LTV-0033-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898602349,"sku":"LTV-0033-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898635117,"sku":"LTV-0033-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898667885,"sku":"LTV-0033-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310898700653,"sku":"LTV-0033-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310898733421,"sku":"LTV-0033-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0033-CEBPB-Reporter-Fig1.jpg?v=1776934149"},{"product_id":"foxc2-ets-reporter-lentivirus-bhv19400227","title":"FOXC2\/ETS Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXC2 is a forkhead box transcription factor essential for developmental patterning and tissue maintenance, including lymphatic vessel formation and the development of the lungs, eyes, kidneys, urinary tract, and cardiovascular system. ETS family transcription factors, such as the endothelial regulator ETV2, control endothelial and mesodermal gene programs. FOXC2 and ETS factors can act together at composite regulatory elements, where their synergistic binding drives target genes involved in vascular and lymphatic development. This cooperative FOXC2-ETS activity is important for endothelial differentiation and has relevance to angiogenesis, lymphangiogenesis, and developmental disorders, making the combined transcriptional output an informative readout in vascular biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXC2\/ETS Reporter Lentivirus is a transcription-factor reporter system that measures the combined activity of the forkhead factor FOXC2 and the ETS family member ETV2. The construct uses tandem repeats of FOXC2 binding elements derived from the MEF2 enhancer to detect their synergistic transcriptional activity and the activation of related signaling pathways. A fluorescent or luminescent reporter, chosen from options including GFP, RFP, mCherry, BFP2, and luciferase configurations, is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying endothelial and lymphatic development and FOXC2-ETS cooperation. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXC2 \u0026amp; ETS family members synergistic activity transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616244077,"sku":"LTV-0118-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362029789549,"sku":"LTV-0118-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362029822317,"sku":"LTV-0118-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362029855085,"sku":"LTV-0118-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362029887853,"sku":"LTV-0118-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362029920621,"sku":"LTV-0118-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXC2-ETV2-TAG-PURO-FIG1-1.jpg?v=1776934147"},{"product_id":"cd40-nf-kappa-b-reporter-lentivirus-bhv19400263","title":"CD40\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD40 is a cell surface receptor of the tumor necrosis factor receptor superfamily, expressed prominently on antigen-presenting cells such as B cells, macrophages, and dendritic cells. Engagement of CD40 by its ligand CD40L, expressed on activated T cells, delivers a critical co-stimulatory signal that licenses antigen-presenting cells, promotes B cell proliferation and antibody class switching, and enhances dendritic cell maturation. CD40 signaling proceeds through TRAF adaptor proteins to activate the transcription factor NF-κB, driving expression of genes that coordinate adaptive immune responses. CD40 is studied for its central role in T cell-dependent immunity and is an actively pursued target for cancer immunotherapy and vaccine development.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CD40\/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human CD40 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable cell line that responds quantitatively to CD40 engagement with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antigen-presenting cell function, immune regulation, and CD40-targeting therapeutics in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CD40 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616276845,"sku":"TRV-0014-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"h-m-ahr-shrna-lentivirus-bhv19400209","title":"h\/m AHR shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eAHR (aryl hydrocarbon receptor) is a ligand-activated, basic helix-loop-helix-PAS transcription factor. In its inactive state it resides in the cytosol bound to chaperone proteins. Upon binding ligands, including environmental xenobiotics such as 2,3,7,8-tetrachlorodibenzo-p-dioxin as well as dietary and microbial metabolites, AHR translocates to the nucleus and dimerizes with the AHR nuclear translocator to activate genes encoding xenobiotic-metabolizing enzymes. Beyond detoxification, AHR regulates immune cell differentiation, intestinal barrier function, cell proliferation, and inflammatory responses. Dysregulated AHR signaling is implicated in toxic responses, chronic inflammation, autoimmune conditions, and cancer, making the receptor an important target in toxicology, immunology, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m AHR shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse AHR for stable, RNA interference-based gene silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and an antibiotic selection marker (Zeocin, Puromycin, or Blasticidin) for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation. Sets supply lentivirus from two independent shRNAs plus a scrambled-shRNA control, supporting loss-of-function studies of AHR in cancer and cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting AHR delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Zeocin, Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616309613,"sku":"LSV-0064-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53362027692397,"sku":"LSV-0064-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Zeocin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53362027725165,"sku":"LSV-0064-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Zeocin \/ 5x10^6","offer_id":53362027757933,"sku":"LSV-0064-2S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hm-AHR-shRNA-Lentivirus-Figure-1.jpg?v=1776934141"},{"product_id":"mouse-pdl1-shrna-lentivirus-bhv19400159","title":"Mouse PDL1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePD-L1 (programmed death-ligand 1, also called B7-H1, encoded by Cd274) is a transmembrane immune checkpoint ligand expressed on tumor cells, antigen-presenting cells, and other tissues. By engaging the inhibitory receptor PD-1 on activated T cells, PD-L1 delivers a suppressive signal that limits T cell proliferation, cytokine production, and cytotoxic activity, contributing to peripheral tolerance and prevention of excessive immune responses. Tumors frequently upregulate PD-L1, often in response to interferon-gamma, to evade immune surveillance. The PD-1\/PD-L1 axis is a central target of checkpoint-blockade immunotherapy, making PD-L1 an important focus of cancer immunology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Mouse PD-L1 shRNA Lentivirus delivers validated short hairpin RNA targeting mouse Cd274 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% PD-L1 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of immune checkpoint signaling, tumor immune evasion, and checkpoint-blockade research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting Cd274 (NCBI Accession: NM_021893.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616342381,"sku":"LSV-0043-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311253840237,"sku":"LSV-0043-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311253873005,"sku":"LSV-0043-5-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311253905773,"sku":"LSV-0043-5-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311253938541,"sku":"LSV-0043-5-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311253971309,"sku":"LSV-0043-5-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311254004077,"sku":"LSV-0043-5-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311254036845,"sku":"LSV-0043-5-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311254069613,"sku":"LSV-0043-5-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311254102381,"sku":"LSV-0043-5-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0043-m-Pdl1-sh-fig1.jpg?v=1776934150"},{"product_id":"atf2-reporter-lentivirus-bhv19400236","title":"ATF2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eATF2 (activating transcription factor 2), also known as CREB2, is a member of the bZIP family of transcription factors. It binds specific DNA elements, often as a homodimer or in heterodimers with partners such as c-Jun, to regulate target gene transcription. ATF2 is a key effector of stress-activated signaling: pro-inflammatory cytokines, DNA-damaging agents, and ultraviolet radiation activate the JNK and p38 MAPK cascades, which phosphorylate ATF2 and stimulate its transcriptional activity. Through these inputs, ATF2 controls genes involved in stress responses, inflammation, cell cycle, and apoptosis, and its dysregulation has been linked to cancer and inflammatory disease.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ATF2 Reporter Lentivirus is a lentiviral reporter system that detects transcriptional activity mediated by ATF2 in mammalian cells. The construct contains tandem repeats of consensus ATF2 DNA-binding elements within the JNK signaling pathway, driving expression of a fluorescent (GFP, RFP, BFP, mCherry, EGFP, d2GFP) or luminescent (firefly, Renilla, or Gaussia luciferase) reporter. It provides a sensitive readout of ATF2 activation in response to stress stimuli such as cytokines, DNA damage, and UV radiation. A range of antibiotic selection markers (blasticidin, hygromycin, puromycin, zeocin) supports establishment of stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it ensures efficient transduction of difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the ATF2\/JNK Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"ATF2-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251616407917,"sku":"LTV-0129-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362088411501,"sku":"LTV-0129-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362088444269,"sku":"LTV-0129-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"ATF2-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362088477037,"sku":"LTV-0129-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362088509805,"sku":"LTV-0129-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362088542573,"sku":"LTV-0129-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362088575341,"sku":"LTV-0129-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"ATF2-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362088608109,"sku":"LTV-0129-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"ATF2-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362088640877,"sku":"LTV-0129-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"ATF2-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362088673645,"sku":"LTV-0129-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"ATF2-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362088706413,"sku":"LTV-0129-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ATF2-TAG-Puro.jpg?v=1776934147"},{"product_id":"h-m-acsl4-shrna-lentivirus-bhv19400166","title":"h\/m ACSL4 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eAcyl-CoA synthetase long-chain family member 4 (ACSL4) is an enzyme that activates long-chain polyunsaturated fatty acids, particularly arachidonic and adrenic acid, by converting them to their acyl-CoA derivatives for incorporation into membrane phospholipids. Through this preference, ACSL4 shapes the cellular lipidome and is a key determinant of sensitivity to ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation. ACSL4 also participates in lipid metabolism, eicosanoid signaling, and steroidogenesis. Its expression influences tumor cell survival, drug response, and tissue injury, making ACSL4 an important target in ferroptosis, cancer metabolism, and cell death research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m ACSL4 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse ACSL4 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping supports broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% ACSL4 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of ferroptosis, lipid metabolism, and cancer cell survival.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting ACSL4 (NCBI Accession: NM_004458.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616375149,"sku":"LSV-0050-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311266881901,"sku":"LSV-0050-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311266914669,"sku":"LSV-0050-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311266947437,"sku":"LSV-0050-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311266980205,"sku":"LSV-0050-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311267012973,"sku":"LSV-0050-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311267045741,"sku":"LSV-0050-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311267078509,"sku":"LSV-0050-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311267111277,"sku":"LSV-0050-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311267144045,"sku":"LSV-0050-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0050-hm-ACSL4-sh2-GFP.jpg?v=1776934147"},{"product_id":"human-bak-shrna-lentivirus-bhv19400148","title":"Human BAK shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBAK, encoded by BAK1, is a pro-apoptotic effector of the BCL-2 protein family and, together with BAX, executes the intrinsic apoptotic pathway. Following apoptotic signals, activated BAK oligomerizes within the mitochondrial outer membrane and forms pores that permeabilize the membrane, releasing cytochrome c and other factors that trigger caspase activation and cell death. BAK activity is restrained by anti-apoptotic BCL-2 proteins and promoted by BH3-only proteins, placing it at the convergence point of survival and death signals. Dysregulation of BAK-dependent apoptosis contributes to tumorigenesis and resistance to chemotherapy, making it a key target in cancer and cell death research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human BAK shRNA Lentivirus delivers validated short hairpin RNA targeting BAK1 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and the shRNA is validated for at least 70% BAK knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation, suitable for transducing difficult-to-transfect cells. Applications include loss-of-function studies of mitochondrial apoptosis, investigations of cell death regulation, and analysis of chemotherapy resistance mechanisms.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BAK1 (NCBI Accession: NM_001188.4) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616440685,"sku":"LSV-0033-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311257182573,"sku":"LSV-0033-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311257215341,"sku":"LSV-0033-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311257248109,"sku":"LSV-0033-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311257280877,"sku":"LSV-0033-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311257313645,"sku":"LSV-0033-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311257346413,"sku":"LSV-0033-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311257379181,"sku":"LSV-0033-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0033-h-BAK-shfig1-1.jpg?v=1776934220"},{"product_id":"h-m-igfbp2-shrna-lentivirus-bhv19400122","title":"h\/m IGFBP2 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIGFBP2 (insulin-like growth factor binding protein 2) is a secreted member of the IGFBP family that binds insulin-like growth factors IGF-1 and IGF-2 with high affinity, modulating their bioavailability and access to the IGF-1 receptor. Through this control of IGF signaling, IGFBP2 influences cell proliferation, survival, and metabolism, and it also exerts IGF-independent effects via integrin and proteoglycan interactions that affect cell adhesion and migration. IGFBP2 is highly expressed during development and is frequently overexpressed in cancers including glioblastoma, where elevated levels correlate with tumor progression and poor prognosis. It is also studied in metabolic and endocrine contexts for its links to insulin sensitivity and adiposity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m IGFBP2 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse IGFBP2 for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of IGFBP2 using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of IGF signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting IGFBP2 (NCBI Accession: NM_000597) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616473453,"sku":"LSV-0002-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311260590445,"sku":"LSV-0002-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311260623213,"sku":"LSV-0002-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311260655981,"sku":"LSV-0002-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311260688749,"sku":"LSV-0002-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311260721517,"sku":"LSV-0002-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311260754285,"sku":"LSV-0002-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311260787053,"sku":"LSV-0002-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311260819821,"sku":"LSV-0002-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311260852589,"sku":"LSV-0002-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0002-hm-IGFBP2-sh-Fig1.jpg?v=1776934210"},{"product_id":"ere-reporter-lentivirus-bhv19400042","title":"ERE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe estrogen response element (ERE) is a DNA sequence that mediates transcription driven by the estrogen receptor (ER), a ligand-activated nuclear receptor encoded by ESR1. Upon binding estrogens such as estradiol, ER dimerizes, translocates to the nucleus, and binds estrogen response elements to regulate genes controlling reproductive tissue development, bone density, and cardiovascular and metabolic physiology. ER signaling is a central driver of hormone-responsive breast cancer, where it remains a major therapeutic target. ERE-driven reporters give a quantitative readout of estrogen receptor transcriptional activity for endocrinology, steroid hormone, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ERE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem estrogen response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of estrogen receptor activation. Reporter options include EGFP, GFP, mCherry, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring ER signaling pathway activity, characterizing estrogens and anti-estrogens, and screening endocrine-active compounds in endocrinology and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Estrogen receptor (ER) signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616506221,"sku":"LTV-0046-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896701805,"sku":"LTV-0046-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896734573,"sku":"LTV-0046-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896767341,"sku":"LTV-0046-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896800109,"sku":"LTV-0046-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896832877,"sku":"LTV-0046-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896865645,"sku":"LTV-0046-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ERE-TAG-PURO-2.jpg?v=1776934393"},{"product_id":"hoxa9-reporter-lentivirus-bhv19400075","title":"HOXA9 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHOXA9 is a homeodomain-containing transcription factor that plays a critical role in hematopoietic stem and progenitor cell expansion and differentiation. It regulates gene transcription by binding promoter-distal enhancers, frequently in cooperation with cofactors such as MEIS1 and PBX proteins, and influences the expression of genes governing proliferation and self-renewal. HOXA9 expression is normally downregulated during myeloid maturation, but it is frequently deregulated in acute leukemias. In acute myeloid leukemia, diverse upstream genetic alterations converge on HOXA9 overexpression, contributing to a block in differentiation and oncogenic transformation. These roles make HOXA9 an important target in leukemia and hematopoiesis research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HOXA9 Reporter Lentivirus places a reporter gene under the control of tandem consensus HOXA9 DNA-binding elements, providing a sensitive readout of HOXA9 transcriptional activity in human and mouse cells. Reporter options include fluorescent (GFP, EGFP, d2GFP, mCherry, RFP) and luminescent (firefly luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring HOXA9 activity in hematopoietic and leukemia models and screening modulators of HOXA9-driven transcription.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the HOXA9 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616538989,"sku":"LTV-0082-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310895784301,"sku":"LTV-0082-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310895817069,"sku":"LTV-0082-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310895849837,"sku":"LTV-0082-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310895882605,"sku":"LTV-0082-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310895915373,"sku":"LTV-0082-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HOXA9-Reporter-Fig.-1.jpg?v=1776934144"},{"product_id":"hvem-nf-kappa-b-reporter-lentivirus-bhv19400258","title":"HVEM\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHVEM (herpesvirus entry mediator, TNFRSF14) is a tumor necrosis factor receptor superfamily member with a complex role in immune regulation. It serves as a molecular switch that can deliver either stimulatory or inhibitory signals depending on its binding partner: engagement of TNF-family ligands such as LIGHT promotes NF-κB-driven activation, whereas interaction with the immunoglobulin-family receptors BTLA and CD160 transmits inhibitory signals that suppress lymphocyte activation. Through this bidirectional signaling, HVEM helps balance immune activation and tolerance. Its function as a checkpoint and co-stimulatory hub makes HVEM a target of interest in immunotherapy, and NF-κB reporters provide a quantitative readout of HVEM pathway engagement.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HVEM\/NF-κB Reporter Lentivirus is an immunotherapy reporter system for studying HVEM as an immune checkpoint molecule. The construct constitutively expresses the human HVEM receptor; engagement of inhibitory ligands such as BTLA suppresses NF-κB signaling and reduces reporter activity, while agents that block this inhibition restore NF-κB-driven reporter expression. Dual reporters, secreted Gaussia luciferase and a fluorescent protein (GFP or RFP), allow real-time monitoring by luminometry and fluorescence.\u003c\/p\u003e\n\u003cp\u003eApplications include testing immune checkpoint inhibitors that target HVEM signaling and studying lymphocyte regulation. Supplied as high-titer, VSV-G-pseudotyped lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, the product is optimized for difficult-to-transfect primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human HVEM under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616571757,"sku":"TRV-0009-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"h-m-lmod1-shrna-lentivirus-bhv19400207","title":"h\/m LMOD1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eLMOD1 (leiomodin 1) is an actin-binding protein of the tropomodulin family that is highly expressed in smooth muscle cells. It functions as an actin filament nucleator and elongation factor, promoting the assembly and maintenance of the contractile actin cytoskeleton. LMOD1 is a marker of the differentiated, contractile smooth muscle phenotype and contributes to the structural integrity and function of smooth muscle tissues such as the gut and vasculature. Loss-of-function mutations in LMOD1 have been linked to megacystis microcolon intestinal hypoperistalsis syndrome, a disorder of smooth muscle dysfunction. LMOD1 is studied for its roles in smooth muscle differentiation, cytoskeletal organization, and related cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m LMOD1 shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse LMOD1 for stable, RNA interference-based gene silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and an antibiotic selection marker (Blasticidin or Puromycin) for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation. Sets supply lentivirus from two independent shRNAs plus a scrambled-shRNA control, supporting loss-of-function studies of LMOD1 in smooth muscle and cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting LMOD1 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616604525,"sku":"LSV-0062-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53362027561325,"sku":"LSV-0062-1S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LMOD1-shRNA-Figure.jpg?v=1776934143"},{"product_id":"creb-reporter-lentivirus-bhv19400013","title":"CREB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCREB (cAMP response element-binding protein) is a basic leucine zipper transcription factor that activates genes containing the cAMP response element (CRE). CREB is a key downstream effector of the cAMP\/PKA signaling pathway: rising intracellular cAMP activates protein kinase A, which phosphorylates CREB at serine 133, enabling recruitment of the coactivator CBP\/p300 and transcriptional activation. CREB is also regulated by other kinases in response to growth factors and calcium. Through these inputs, CREB controls genes involved in cell proliferation, survival, metabolism, and synaptic plasticity, and its dysregulation is implicated in neurological disorders and cancer, making CRE-driven reporters valuable for studying signal transduction.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CREB Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of cAMP\/PKA pathway activity and CREB-mediated transcription in human or mouse cells. The cAMP response element drives reporter expression in response to cAMP\/PKA activation, so signal intensity reflects endogenous pathway activation. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study cAMP\/PKA signaling, GPCR responses, CREB-dependent transcription, and pathway modulators. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the cAMP\/PKA pathway (CREB-1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616637293,"sku":"LTV-0014-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893425005,"sku":"LTV-0014-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310893457773,"sku":"LTV-0014-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893490541,"sku":"LTV-0014-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310893523309,"sku":"LTV-0014-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310893556077,"sku":"LTV-0014-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893588845,"sku":"LTV-0014-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_b770ab66-5668-4b33-93c0-bf1611a949c7.jpg?v=1776934150"},{"product_id":"foxd1-reporter-lentivirus-bhv19400226","title":"FOXD1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXD1 is a forkhead box transcription factor with a conserved winged-helix DNA-binding domain that regulates genes essential for developmental patterning and tissue maintenance. It has well-defined roles in kidney and retina development, where it directs stromal cell behavior, as well as in lymphatic vessel formation and embryo implantation. Among its target genes is placental growth factor, a VEGF family member involved in angiogenesis. FOXD1 has been associated with several human conditions, including cancer and recurrent pregnancy loss, where altered FOXD1 activity affects tissue development and homeostasis. These functions make FOXD1-dependent transcription an informative readout in developmental and disease biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXD1 Reporter Lentivirus is a transcription-factor reporter system designed to preferentially detect FOXD1 transcriptional activity in human and mouse cells. The construct uses tandem repeats of the FOXD1 DNA-binding element derived from the human placental growth factor promoter, a validated downstream target, to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, and luciferase configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying FOXD1 in kidney and retina development, lymphatic biology, and related disease processes. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXD1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616670061,"sku":"LTV-0117-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362027790701,"sku":"LTV-0117-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362027823469,"sku":"LTV-0117-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362027856237,"sku":"LTV-0117-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362027889005,"sku":"LTV-0117-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362027921773,"sku":"LTV-0117-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXD1-TAG-Puro-Fig1.jpg?v=1776934220"},{"product_id":"cd160-nfat-reporter-lentivirus-bhv19400265","title":"CD160\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD160 is a glycosylphosphatidylinositol-anchored cell surface receptor expressed predominantly on natural killer cells and subsets of T cells. On NK cells, engagement of CD160 by its ligands, including major histocompatibility complex class I molecules and herpesvirus entry mediator, can deliver activating signals that enhance cytotoxicity and cytokine production. CD160 signaling feeds into the calcium-dependent NFAT pathway, a key transcriptional program downstream of immunoreceptor engagement that controls effector gene expression. Depending on cellular context, CD160 has also been described as a co-inhibitory receptor, reflecting its complex role in immune regulation. The receptor is studied for its contributions to NK and T cell activation, immune surveillance, and as a candidate target in immunotherapy.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CD160\/NFAT Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human CD160 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NFAT response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to CD160 engagement with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of NK cell activation, immune responses, and CD160-targeting immunotherapies in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CD160 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616702829,"sku":"TRV-0017-6S","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fkh-reporter-lentivirus-bhv19400035","title":"FKH Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eForkhead (FKH) transcription factors are a large family defined by a conserved forkhead DNA-binding domain that recognizes FKH response elements. Members such as FOXO1, FOXO3, FOXA2, and FOXP1 regulate diverse processes including cell cycle progression, apoptosis, metabolism, oxidative stress resistance, and development. FOXO factors in particular act downstream of the PI3K\/AKT pathway: AKT-mediated phosphorylation drives their nuclear exclusion, while loss of growth factor signaling permits nuclear accumulation and activation of target genes. Forkhead transcription factors are central to aging, immune regulation, and stem cell maintenance, and their dysregulation is implicated in cancer and metabolic disease, making FKH activity a valuable research readout.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FKH Reporter Lentivirus places a reporter gene under the control of tandem forkhead response elements, providing a sensitive readout of forkhead transcription factor DNA-binding activity in human and mouse cells. It detects the activity of factors that bind FKH elements, including FOXO1, FOXO3, FOXA2, and FOXP1. Reporter options include fluorescent (GFP, RFP, EGFP, mCherry) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring FOXO and PI3K\/AKT pathway activity, studying metabolism and stress responses, and screening modulators of forkhead transcription factors.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Forkhead (FKH) Transcription Factors transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616735597,"sku":"LTV-0039-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310901223789,"sku":"LTV-0039-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310901256557,"sku":"LTV-0039-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310901289325,"sku":"LTV-0039-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310901322093,"sku":"LTV-0039-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310901354861,"sku":"LTV-0039-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310901387629,"sku":"LTV-0039-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0039-FKH-Reporter-Fig-2.jpg?v=1776934391"},{"product_id":"esre-reporter-lentivirus-bhv19400031","title":"ESRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ER stress-response element (ESRE) is a DNA sequence that mediates transcriptional induction during the unfolded protein response (UPR), the cell's adaptive program for restoring endoplasmic reticulum protein-folding capacity. ESRE-driven transcription is controlled by the bZIP transcription factors ATF6 and XBP1. Upon ER stress, ATF6 is cleaved to release an active nuclear fragment, while IRE1-mediated splicing of XBP1 mRNA produces the potent activator XBP1s. Together they induce chaperones and folding enzymes that relieve ER stress. The UPR is central to secretory cell function and is implicated in cancer, metabolic disease, and neurodegeneration, making ESRE-driven reporters valuable for studying protein homeostasis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ESRE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem ER stress-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of ATF6 and XBP1 activity in transduced cells. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring the unfolded protein response, studying the IRE1\/XBP1 and ATF6 branches of ER stress signaling, and screening modulators of protein homeostasis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the ER stress response transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616768365,"sku":"LTV-0035-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310897095021,"sku":"LTV-0035-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310897127789,"sku":"LTV-0035-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897160557,"sku":"LTV-0035-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310897193325,"sku":"LTV-0035-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310897226093,"sku":"LTV-0035-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897258861,"sku":"LTV-0035-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_ab1e4f4f-55db-4002-be75-82fbed2b325a.jpg?v=1776934330"},{"product_id":"pd-l1-b7-h1-orf-cdna-lentivirus-bhv19400108","title":"PD-L1 (B7-H1) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD274 encodes PD-L1 (programmed cell death 1 ligand 1, also known as B7-H1), a transmembrane immune checkpoint ligand. By binding the inhibitory receptor PD-1 on T cells, PD-L1 dampens T cell receptor signaling, promoting peripheral tolerance and limiting immune-mediated tissue damage. Many tumors upregulate PD-L1 to suppress anti-tumor T cell responses and evade immune clearance. The PD-1\/PD-L1 axis is a central target of cancer immunotherapy, and checkpoint-blockade antibodies against this pathway are widely used clinically, making CD274 a key focus of immuno-oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PD-L1 (B7-H1) ORF cDNA Lentivirus delivers the PD-L1 (CD274) open reading frame for stable overexpression in mammalian cells. Expression of the ORF, fused to a C-terminal V5 tag, is driven by a CMV or EF1α promoter, with a GFP or RFP reporter and optional blasticidin or puromycin selection marker separated by self-cleaving peptide sequences for independent translation. The lentiviral vectors are optimized for high expression and reliable genome integration; ORF accuracy is confirmed by sequencing and protein expression validated by transient transfection.\u003c\/p\u003e\n\u003cp\u003eSupplied as high-titer, third-generation, VSV-G-pseudotyped particles, it efficiently transduces primary and thawed cells, supporting stable PD-L1-expressing cell lines for studying immune checkpoint signaling and immunotherapy.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of CD274 (NCBI Accession: NM_014143, NM_021893) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-Cd274-V5 \/ RFP\/none \/ 5x10^6","offer_id":53251616801133,"sku":"LCV-0016-6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd274-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311254724973,"sku":"LCV-0016-5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311254757741,"sku":"LCV-0016-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311254790509,"sku":"LCV-0016-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-Flag \/ none\/Puromycin \/ 5x10^6","offer_id":53311254823277,"sku":"LCV-0016-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-V5 \/ none\/Blasticidin \/ 5x10^6","offer_id":53311254856045,"sku":"LCV-0016-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-Flag \/ none\/Blasticidin \/ 5x10^6","offer_id":53311254888813,"sku":"LCV-0016-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cas9-lentivirus-bhv19400193","title":"Cas9 Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCas9 is an RNA-guided DNA endonuclease derived from the bacterial CRISPR adaptive immune system. When complexed with a guide RNA, Cas9 recognizes a complementary genomic sequence adjacent to a protospacer-adjacent motif and introduces a double-strand break, which the cell repairs through error-prone non-homologous end joining or templated homology-directed repair. This programmability has made Cas9 a foundational tool for targeted gene knockout, knock-in, and editing across virtually all model systems. Stable expression of Cas9 in mammalian cells is often a prerequisite for efficient genome editing and for pooled CRISPR screens, making constitutive Cas9 delivery a widely used research resource.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Cas9 Lentivirus delivers high-titer lentiviral particles pre-packaged with an expression vector for Cas9 together with a fluorescent reporter or drug-selection marker for convenient identification of transduced cells. A PGK promoter drives Cas9, and the downstream reporter or selection marker is separated by a P2A self-cleaving peptide, allowing expression of two distinct proteins from a single mRNA. ORF cloning accuracy has been confirmed by sequencing, and protein expression has been validated by transient transfection. The product supports stable Cas9 expression for targeted genome editing and CRISPR screening, and efficiently transduces difficult-to-transfect cells, including primary and thawed cells. Supplied purified by PEG precipitation and sucrose gradient centrifugation, with each vial containing 380-500 uL at a total titer of 1x10^6 TU.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of Cas9 in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV, EF1a, PGK). Reporter and selection marker components (BFP, Firefly Luc, GFP, RFP; Blasticidin, Bleomycin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"PGK-SpCas9-GFP \/ GFP\/none \/ 1x10^6","offer_id":53251616833901,"sku":"LC9-0001-1","price":895.0,"currency_code":"USD","in_stock":true},{"title":"PGK-SpCas9-RFP \/ RFP\/none \/ 1x10^6","offer_id":53362030346605,"sku":"LC9-0001-2","price":895.0,"currency_code":"USD","in_stock":true},{"title":"PGK-SpCas9-Puro \/ none\/Puromycin \/ 1x10^6","offer_id":53362030379373,"sku":"LC9-0001-3","price":895.0,"currency_code":"USD","in_stock":true},{"title":"PGK-SpCas9-BSD \/ none\/Blasticidin \/ 1x10^6","offer_id":53362030412141,"sku":"LC9-0001-4","price":895.0,"currency_code":"USD","in_stock":true}]},{"product_id":"brn3-reporter-lentivirus-bhv19400246","title":"BRN3 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBRN3A (POU4F1) and BRN3B (POU4F2) are class IV POU-domain transcription factors that bind DNA through a bipartite POU domain. Categorized as non-octamer-binding proteins because of their reduced affinity for canonical octamer motifs, they instead recognize distinct BRN3 elements such as those found in the corticotropin-releasing hormone promoter. BRN3A is expressed in retinal ganglion cells, peripheral sensory neurons, and lymphoid lineages, where it directs sensory neuron differentiation and survival, retinal ganglion cell development, and aspects of immune function. BRN3B is similarly important for retinal ganglion cell specification and axon guidance. These factors are studied as regulators of neuronal development and visual system biology and are of interest in neuroscience and developmental research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe BRN3 Reporter Lentivirus is a transcription factor reporter system that detects transcriptional activity mediated by BRN3A and BRN3B in mammalian cells. The construct contains tandem repeats of BRN3 DNA-binding elements derived from the rat CRH promoter, placed upstream of a minimal promoter to drive a fluorescent or luminescent reporter. An optimized upstream enhancer maximizes signal-to-noise, and a constitutive drug selection marker (Blasticidin, Hygromycin, Puromycin, or Zeocin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for readout by fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying BRN3 activity in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the BRN3 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"BRN3-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251616899437,"sku":"LTV-0139-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362090901869,"sku":"LTV-0139-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362090934637,"sku":"LTV-0139-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BRN3-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362090967405,"sku":"LTV-0139-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362091000173,"sku":"LTV-0139-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362091032941,"sku":"LTV-0139-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362091065709,"sku":"LTV-0139-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BRN3-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362091098477,"sku":"LTV-0139-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BRN3-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362091131245,"sku":"LTV-0139-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BRN3-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362091164013,"sku":"LTV-0139-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BRN3-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362091196781,"sku":"LTV-0139-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/brn3-2.jpg?v=1776934148"},{"product_id":"c-myc-reporter-lentivirus-bhv19400006","title":"c-MYC Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ec-MYC is a basic helix-loop-helix leucine zipper transcription factor and a master regulator of cell growth, proliferation, metabolism, and ribosome biogenesis. By dimerizing with MAX and binding E-box elements in target promoters, c-MYC activates and represses large gene networks that coordinate the cell cycle and biosynthetic capacity. Its expression is tightly controlled in normal cells, but c-MYC is one of the most commonly deregulated oncogenes in human cancer. Aberrant c-MYC activity drives uncontrolled proliferation, metabolic reprogramming, and tumor progression, making it a central focus of oncology and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe c-MYC Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of c-MYC transcriptional activity in human or mouse cells. Reporter expression is controlled by c-MYC-responsive elements, so signal intensity reflects endogenous c-MYC activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study c-MYC-driven proliferation, metabolism, and transformation, and to screen modulators of c-MYC pathway activity. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the cMYC transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616866669,"sku":"LTV-0007-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896537965,"sku":"LTV-0007-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896570733,"sku":"LTV-0007-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896603501,"sku":"LTV-0007-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896636269,"sku":"LTV-0007-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896669037,"sku":"LTV-0007-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0007-Fig1-MYC-TAG-Puro-1.jpg?v=1776934330"},{"product_id":"drd2-orf-cdna-lentivirus-bhv19400197","title":"DRD2 ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eDRD2 encodes the dopamine receptor D2, a G protein-coupled receptor that mediates the actions of the neurotransmitter dopamine. As a member of the D2-like receptor family, it couples primarily to inhibitory Gi\/o proteins, reducing intracellular cyclic AMP and modulating ion channel activity and downstream signaling. DRD2 is expressed in brain regions controlling movement, motivation, reward, and neuroendocrine function, and it acts both postsynaptically and as a presynaptic autoreceptor regulating dopamine release. The receptor is a principal target of antipsychotic drugs and is implicated in schizophrenia, Parkinson disease, addiction, and prolactin regulation, making DRD2 an important subject in neuroscience and pharmacology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe DRD2 ORF cDNA Lentivirus enables stable overexpression of human dopamine receptor D2 in mammalian cells. Lentiviral particles are packaged with an expression vector encoding the DRD2 open reading frame driven by a CMV promoter, delivered through a third-generation, VSV-G pseudotyped system. Depending on configuration, the construct may include a C-terminal epitope tag, a fluorescent reporter (such as EGFP or mCherry), and an antibiotic selection marker (Blasticidin or Puromycin), with tag, reporter, and selection elements linked by self-cleaving peptide sequences to allow independent translation. Stable polyclonal cell lines are readily established by antibiotic selection or fluorescence-based sorting. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product transduces primary and difficult-to-transfect cells for gain-of-function studies of DRD2 in receptor signaling and pharmacology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of DRD2 (NCBI Accession: NM_000795) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (EGFP, GFP, mCherry, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-DRD2-V5 \/ GFP\/none \/ 3x10^6","offer_id":53251616932205,"sku":"LCV-0045-001","price":695.0,"currency_code":"USD","in_stock":true}]},{"product_id":"foxf1-reporter-lentivirus-bhv19400087","title":"FOXF1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXF1 is a member of the Forkhead box family of transcription factors with essential roles in mesenchymal development, particularly of the lung, gut, and vasculature. It cooperates with other transcription factors to regulate tissue patterning and is required for normal lung morphogenesis. FOXF1 acts with ETS factors and SMAD4 to drive expression of genes such as ACVRL1. FOXF1 has been implicated in cancer progression and metastasis of lung and breast cancers and is required for the oncogenic properties of the PAX3-FOXO1 fusion in rhabdomyosarcoma, making it relevant to developmental and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXF1 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of FOXF1\/Activin Responsive Elements (FARE), which contain FOXF1, ETS, and SMAD4 DNA-binding elements derived from the ACVRL1 promoter. The elements drive a reporter gene downstream of a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXF1 transcriptional activity. A wide range of reporters is available, including EGFP, mCherry, BFP2, d2GFP, GFP, RFP, firefly luciferase, Gaussia luciferase, and Renilla luciferase, with selection markers including blasticidin, hygromycin, puromycin, and zeocin. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying FOXF1 activity in development and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXF1\/FARE transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616964973,"sku":"LTV-0096-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251633807725,"sku":"LTV-0096-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53251633840493,"sku":"LTV-0096-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53251633873261,"sku":"LTV-0096-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53251633906029,"sku":"LTV-0096-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53251633938797,"sku":"LTV-0096-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Puromycin \/ 5x10^6","offer_id":53251633971565,"sku":"LTV-0096-15S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Blasticidin \/ 5x10^6","offer_id":53251634004333,"sku":"LTV-0096-16S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXF1-Panel.jpg?v=1776934578"}],"url":"https:\/\/www.ebiohippo.com\/collections\/lentiviral-vectors.oembed?page=3","provider":"BioHippo","version":"1.0","type":"link"}