{"title":"Liver Cancer Cell Lines","description":"\u003cp\u003eValidated human liver cancer cell lines — HepG2, HuH7, Hep3B, PLC\/PRF\/5, SK-HEP-1 and the SNU series — for hepatocellular carcinoma, viral hepatitis, drug metabolism and hepatotoxicity research.\u003c\/p\u003e","products":[{"product_id":"hep3b-cell-bhc11101068","title":"HEP3B cell","description":"The Hep3B cell line, derived from an 8-year-old child with liver cancer, is a pivotal model in the study of human liver cancer cells and their responses to various therapeutic agents. Hep3B cells contain an integrated hepatitis B virus genome and is integral in the investigation of differential drug responses due to its unique genetic and phenotypic characteristics.\nThe Hep 3B human hepatoma cell line is renowned for its extensive expression of liver-specific proteins such as alpha-fetoprotein (AFP), albumin, and various other markers, making it an invaluable tool in drug metabolism and hepatotoxicity studies. This wide array of expressed proteins allows for a comprehensive assessment of how liver cancer cells interact with and metabolize therapeutic agents.\nThe Hep 3B cell line and its derivative cell lines enable the tracking of tumor growth and metastasis in vivo, facilitating the study of liver cancer progression and the efficacy of potential treatments.\nThe Hep3B cell line stands out as a crucial resource for advancing our understanding of liver cancer biology and the development of more effective therapeutic strategies.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101068\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950206316909,"sku":"305141","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HEP3B_20P1_20300492-M_2010x01_2012092022_1920x1920_6ab45186-3fc8-471b-950e-54c4f18d52f9.jpg?v=1769069051"},{"product_id":"hepg2-cell-bhc11100234","title":"HepG2 cell","description":"HepG2 cells, a hepatoblastoma cell line, are a cornerstone in biological science, particularly in liver cancer research. The HepG2 cell line was first isolated in 1975 and initially misclassified as hepatocellular carcinoma, with the HepG2 cell line origin as hepatoblastoma being recognized later, clarifying years of scientific ambiguity.\nHuman hepatic cell lines such as HepG2 are commonly used as in vitro models for primary human hepatocytes. These cell lines offer advantages such as indefinite proliferation, stable phenotype, easy accessibility, and ease of manipulation. However, they exhibit reduced expression of some metabolic functions compared to primary hepatocytes. Derived from hepatocellular carcinoma, HepG2 cells proliferate quickly and have an epithelial-like morphology, performing many specialized hepatic functions. Despite these differences, HepG2 cells are widely used in studying drug metabolism and toxicity, thanks to their resemblance to hepatocellular carcinoma and hepatoblastoma cells in terms of drug metabolism and transport proteins.\nHepG2 is a human liver cancer cell line often used in research, including studies on drug metabolism and toxicity. However, one of the limitations of hepatoma HepG2 cells is their altered expression of certain liver-specific functions, including the expression of cytochrome P450 enzymes. Cytochrome P450 enzymes are essential for the metabolism of xenobiotics (foreign compounds such as drugs and carcinogens) in the liver. The altered or reduced expression of these enzymes in HepG2 cells can affect their ability to accurately model the metabolism and elimination of xenobiotics, which is a critical aspect of liver function.\nThe HepG2 cell line, alongside other hepatoma cell lines such as the Hep3B and human hepatoma HepaRG cell lines, contributes to a broader understanding of human liver carcinoma cells. The cell line stands out for its versatility, serving as an optimal choice for stable cell line generation, transfection studies, drug metabolism, and hepatotoxicity studies. Furthermore, the HepG2 cell line is pivotal in a range of applications, from 3D cell culture to high-throughput screening and toxicology.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100234\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950207365485,"sku":"300198","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HepG2_20waKo1_20P1_2020x01_20060625_ch00_1920x1920_61eef594-0083-4771-abc6-2502c1646880.jpg?v=1769069057"},{"product_id":"hroc395met1-cell-bhc11101672","title":"HROC395Met1 cell","description":"The HROC (Hansestadt Rostock Colorectal cancer) cell line panel comprises patient-derived colorectal cancer models developed from primary tumor tissue and\/or matched metastatic lesions. These cell lines are frequently accompanied by corresponding patient-derived xenografts (PDXs) and organoids, enabling integrative modeling of colorectal cancer (CRC) in both in vitro and in vivo systems. HROC models preserve critical clinical and molecular diversity found in colorectal cancer, including variations in microsatellite instability (MSI vs. MSS) and key genetic drivers such as mutations in APC, KRAS, BRAF, PIK3CA, and TP53. Cultured as adherent epithelial monolayers and typically used at low passage numbers, HROC lines maintain phenotypic and genomic fidelity to their patient tumors, supporting translational relevance in drug and biomarker research.\n\nThe nomenclature system for HROC cell lines provides detailed metadata on origin and experimental history. For example, “Tu” identifies cell lines derived from primary tumors, “Met” from metastatic lesions, while “T#” and “M#” indicate the number of PDX transfers and the specific mouse host, respectively. This systematic naming allows for easy tracking of matched sets, such as primary-metastasis pairs or in vitro-in vivo derivatives. These matched models support studies on clonal evolution, metastasis, therapy resistance, and pharmacokinetic behavior—including transporter expression and barrier integrity relevant for drug absorption. Cell lines undergo routine authentication (e.g., STR profiling) and are tested regularly for mycoplasma contamination. Characterization data for numerous HROC models are publicly available in Cellosaurus and in peer-reviewed publications.\n\nHROC cell lines are particularly valuable for subtype-stratified drug screening, biomarker discovery across MSI-H and MSS tumors, and mechanistic studies involving primary vs. metastatic disease. When paired with PDXs and\/or organoids, they provide a robust platform for preclinical evaluation, including drug sensitivity testing and modeling of tumor-stroma or immune interactions. Due to their comprehensive annotation and clinical relevance, HROC models are suitable for both basic and translational research in colorectal cancer.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101672\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950210543981,"sku":"300854","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HROC395Met1_20WAKO_20P1_2020x01_20220825_ch00_1920x1920_0b76726f-e1c6-4060-84d2-d373e818f7b5.jpg?v=1769069091"},{"product_id":"hrohep03-cell-bhc11100118","title":"HROHep03 cell","description":"This is one cell line of a series of tumor cell lines which have been established by PD Dr. Michael Linnebacher since 2006.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100118\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950211625325,"sku":"300197","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hrohep03-_282_29_1920x1920_9c299ce4-8142-4370-b52a-8920226354bd.jpg?v=1769069104"},{"product_id":"hucc-t1-cell-bhc11101112","title":"HuCC-T1 cell","description":"HuCC-T1 is a human cholangiocarcinoma cell line established from an intrahepatic bile duct carcinoma. Cholangiocarcinoma is a highly aggressive malignancy with limited treatment options and a poor prognosis. HuCC-T1 cells have been utilized extensively in research to study the pathophysiology of cholangiocarcinoma and to explore potential therapeutic approaches. The cell line is particularly valuable in studying the effects of various chemotherapeutic agents, including statins, which have shown potential in suppressing the proliferation of cholangiocarcinoma cells.\n\nIn studies involving HuCC-T1, statins such as pitavastatin and atorvastatin were observed to significantly inhibit cell proliferation, particularly when combined with conventional chemotherapeutic agents like gemcitabine, cisplatin, and 5-fluorouracil (5-FU). The combination of these drugs resulted in enhanced suppression of cell growth, indicating potential synergistic effects. The mechanism of action involves the induction of apoptosis via suppression of the MAPK\/ERK signaling pathway, as evidenced by increased levels of cleaved caspase-3 and reduced levels of phosphorylated ERK (p-ERK). These findings suggest that statins may serve as a promising adjunct therapy in the treatment of cholangiocarcinoma, potentially improving outcomes when used alongside existing anticancer drugs.\n\nFurthermore, the HuCC-T1 cell line has been characterized for various molecular markers, including p53 gene status, which plays a critical role in cell cycle regulation and apoptosis. The precise p53 mutation status in HuCC-T1 could provide insights into the cell line's response to DNA-damaging agents and its overall tumorigenic potential. Given its molecular characteristics, HuCC-T1 continues to be a pivotal tool in cholangiocarcinoma research, offering insights into the disease's molecular underpinnings and aiding in the development of novel therapeutic strategies.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101112\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950212149613,"sku":"300469","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HuCCT-1_20P1_20300469_2010x01_20020323_1920x1920_178a6f7d-b98d-40a0-b046-cd1ae8731bcf.jpg?v=1769069110"},{"product_id":"huh-6-cell-bhc11101101","title":"HuH-6 cell","description":"The HuH-6 cell line is a human hepatoblastoma cell line derived from the liver tissue of a child diagnosed with hepatoblastoma, a rare malignant liver tumor that primarily affects pediatric patients. HuH-6 cells exhibit characteristics typical of hepatic lineage, including the expression of hepatocyte-associated markers such as alpha-fetoprotein (AFP), albumin, and cytokeratins. These cells are adherent in culture and display epithelial morphology, making them a valuable in vitro model for studying liver development, hepatoblastoma pathogenesis, and liver-specific metabolic functions.\n\nHuH-6 cells are particularly useful in research focused on pediatric liver cancers, as they retain many of the molecular features observed in primary hepatoblastoma tissues. These include the activation of Wnt\/β-catenin signaling, a pathway frequently implicated in hepatoblastoma tumorigenesis. The cell line has also been employed in studies investigating the effects of chemotherapeutic agents, drug metabolism, and resistance mechanisms, as well as in the exploration of gene expression profiles associated with tumor progression and differentiation. Due to their reproducibility and consistent growth characteristics, HuH-6 cells serve as a robust model system for both basic liver cancer research and preclinical drug screening.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101101\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950212182381,"sku":"305092","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HuH-6_20P1_2020x01_20150725_ch00_1920x1920_0912fe2c-6130-4028-8204-75e74f000e00.jpg?v=1769069111"},{"product_id":"huh7-cell-bhc11101666","title":"HuH7 cell","description":"HuH-7 cells are a type of epithelial-like, tumorigenic cell line initially taken from a liver tumor in a 57-year-old Japanese male in 1982. The human hepatoma-derived HuH-7 cell line and its derivatives have been widely used in research as a convenient experimental substitute for primary hepatocytes. In particular, they have been instrumental in hepatitis C research and used as host cells for propagating the virus in vitro. HuH-7 cells have played a crucial role in hepatitis C research, especially when it comes to drug development. Prior to 2005, researchers were unable to cultivate the hepatitis C virus in the laboratory, making it difficult to test potential drug candidates against it.\nThe introduction of the HuH-7 cell line changed that. These cells are highly permissive to the replication of the hepatitis C virus, making them ideal for in vitro testing. By using the HuH-7 cells, researchers were able to screen drug candidates against laboratory-grown hepatitis C, which paved the way for the development of new drugs to fight the virus. Unlike other established human hepatoma cell lines, HuH-7 cells can be propagated in a chemically defined medium containing trace amounts of selenium in place of serum. This allows for systematic studies of the in vitro effects of various compounds on their growth and metabolism.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101666\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950212215149,"sku":"300156","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/huh7-_281_29__281_29.jpg?v=1769069111"},{"product_id":"li-7-cell-bhc11101026","title":"Li-7 cell","description":"The Li-7 cell line is a human hepatocellular carcinoma (HCC) cell line that is commonly used in cancer research, particularly in the study of liver cancer. Derived from a primary liver tumor, Li-7 cells exhibit the typical characteristics of HCC, including the ability to produce alpha-fetoprotein (AFP), a marker often elevated in liver cancer. These cells are also known for their genetic stability, which makes them a reliable model for long-term studies.\nGenomic analysis of Li-7 cells has revealed various chromosomal abnormalities that are characteristic of HCC, including gains in regions such as 5p, 8q, and 11q, and losses in 13q and 14q. These chromosomal changes are indicative of the complex genetic alterations that drive hepatocarcinogenesis. Specifically, the gain in 8q is associated with the amplification of the MYC oncogene, which plays a crucial role in cell cycle progression and proliferation, further emphasizing the utility of Li-7 cells in oncogenic pathway studies.\nLi-7 cells also serve as a valuable model for studying the molecular mechanisms underlying HCC, including the pathways involving key genes like TFDP1, CUL4A, and CDC16, which have been identified as targets of amplification in HCC. These genes are involved in cell cycle regulation and DNA repair, processes that are often dysregulated in cancer. Thus, the Li-7 cell line is instrumental in elucidating the molecular events that lead to the development and progression of liver cancer, providing insights that could guide therapeutic strategies.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101026\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950214574445,"sku":"305102","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Li-7_20_285_29_1920x1920_2a19a9ee-2a25-44ae-aec9-2885b1ab7f51.jpg?v=1769069133"},{"product_id":"mahlavu-cell-bhc11101102","title":"Mahlavu cell","description":"The Mahlavu cell line is a human hepatocellular carcinoma (HCC) cell line derived from an adult patient with liver cancer. Hepatocellular carcinoma is the most common type of primary liver cancer, often associated with chronic liver disease, including hepatitis B or C infection and cirrhosis. Mahlavu cells exhibit characteristics typical of aggressive liver cancer, such as high proliferative capacity, invasive behavior, and resistance to apoptosis, making them a valuable model for studying the molecular mechanisms underlying HCC progression and for testing potential anti-cancer therapies.\n\nMahlavu cells are known for their epithelial morphology and are typically cultured in conditions that support the growth of hepatic cells. These cells possess mutations in key oncogenes and tumor suppressor genes, which contribute to their tumorigenic properties. Researchers often use Mahlavu cells to study signaling pathways involved in HCC, such as the Wnt\/β-catenin pathway, which is frequently dysregulated in liver cancers. Additionally, this cell line is useful in drug resistance studies, as it can provide insights into the mechanisms by which HCC cells evade standard chemotherapy treatments.\n\nDue to its aggressive nature, the Mahlavu cell line is also employed in metastasis research. Studies involving these cells can help elucidate the processes by which liver cancer spreads to other organs, particularly the lungs and lymph nodes.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101102\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950215360877,"sku":"300473","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Mahlavu_20P1_2020x01_20121023_ch00_1920x1920_a5baaec7-d018-4052-8759-0b0334f66163.jpg?v=1769069141"},{"product_id":"plc-prf-5-cell-bhc11100131","title":"PLC\/PRF\/5 cell","description":"The cells produce HBsAg. At present, there is no evidence that this cell line produces infectious Hepatitis B virus.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100131\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950221422957,"sku":"300315","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/PLC-PRF-5_20WaKo_20P1_2020x01_20040925_ch00_1920x1920_02f46cb3-8331-4131-bb96-c7fb272dae64.jpg?v=1769069195"},{"product_id":"rbe-cell-bhc11101111","title":"RBE cell","description":"The RBE cell line is a human intrahepatic cholangiocarcinoma (CC) cell line derived from a 64-year-old female patient. This cell line was established alongside a sarcomatoid counterpart (SSP-25) from the same tumor nodule, highlighting the coexistence of adenocarcinoma and sarcomatoid components within a single CC lesion. RBE cells are characterized by their epithelial morphology, growing as a monolayer with a Epithelial-like appearance, which is typical of epithelial cells.\n\nPhenotypically, the RBE cell line expresses key markers associated with cholangiocarcinoma. These include cytokeratins CK7 and CK19, gamma-glutamyl transpeptidase (GGT), carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and vimentin. Additionally, mucin production is detected in approximately half of the RBE cells, as evidenced by periodic acid-Schiff (PAS) staining. These features confirm the adenocarcinoma origin of RBE cells and distinguish them from the SSP-25 cell line, which lacks CEA, CA19-9, and mucin expression.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101111\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950221783405,"sku":"305019","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/RBE_20P0_20305019-M_20konfluent_2010x01_20080123_1920x1920_f1f42536-3962-4649-8ea5-edd2c08f4f11.jpg?v=1769069197"},{"product_id":"sk-hep-1-cell-bhc11100155","title":"SK-HEP-1 cell","description":"The SK-HEP-1 cell line is a cancer cell line derived from a liver adenocarcinoma in a 52-year old Caucasian man. It has been shown to form tumors in immunocompromised mice, produce fibronectin, alpha-1 protease inhibitor, and Interleukin-1. However, there is an alternative hypothesis that the cells are of endothelial origin and not hepatocytes.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100155\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950224109933,"sku":"300334","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SK-HEP-1_20WaKo_20P1_2020x01_20100725_ch00_1920x1920_369e34ce-8c84-4432-8a1d-b25e0ef49172.jpg?v=1769069223"},{"product_id":"snu-182-cell-bhc11101359","title":"SNU-182 cell","description":"The SNU-182 cell line is derived from a human hepatocellular carcinoma (HCC), which is a primary malignancy of the liver. This cell line is widely used in liver cancer research to study the molecular and cellular mechanisms underlying hepatocarcinogenesis, tumor progression, and therapeutic responses. Hepatocellular carcinoma is one of the most common and deadly forms of liver cancer, making cell lines like SNU-182 essential for advancing our understanding of the disease and developing effective treatments.\nSNU-182 cells exhibit an epithelial morphology and express markers typical of liver cancer, such as alpha-fetoprotein (AFP) and hepatocyte-specific antigens. They harbor genetic and epigenetic alterations that are frequently observed in HCC, including mutations in key oncogenes and tumor suppressor genes. Researchers use SNU-182 cells to explore various signaling pathways involved in liver cancer, such as the Wnt\/β-catenin, PI3K\/Akt, and MAPK pathways. These cells are also employed in high-throughput drug screening assays and preclinical testing of chemotherapeutic agents, targeted therapies, and combination treatments. Additionally, SNU-182 cells are utilized to study mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the SNU-182 cell line in hepatocellular carcinoma research highlights its importance in advancing our knowledge of liver cancer biology and in the development of new therapeutic approaches for patients with HCC.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101359\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950225027437,"sku":"305119","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/snu-182_20_282_29_1920x1920_655cf403-8b90-454a-9c99-50b4d734785d.jpg?v=1769069232"},{"product_id":"snu-387-cell-bhc11101360","title":"SNU-387 cell","description":"The SNU-387 cell line is derived from a human hepatocellular carcinoma (HCC) and is widely utilized in liver cancer research. This cell line provides a valuable model for studying the molecular and cellular mechanisms of hepatocarcinogenesis, tumor progression, and therapeutic responses. Hepatocellular carcinoma is one of the most common and lethal forms of liver cancer, making cell lines like SNU-387 essential for advancing our understanding of the disease and developing effective treatments.\nSNU-387 cells exhibit an epithelial morphology and express markers typical of liver cancer, such as alpha-fetoprotein (AFP) and hepatocyte-specific antigens. They are characterized by genetic and epigenetic alterations common in HCC, including mutations in key oncogenes and tumor suppressor genes. Researchers use SNU-387 cells to investigate signaling pathways involved in liver cancer, such as the Wnt\/β-catenin, PI3K\/Akt, and MAPK pathways. These cells are also employed in high-throughput drug screening assays and preclinical testing of chemotherapeutic agents and targeted therapies. Additionally, SNU-387 cells are used to study the mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the SNU-387 cell line in hepatocellular carcinoma research highlights its importance in advancing our knowledge of liver cancer biology and in the development of new therapeutic approaches for HCC patients.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101360\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950225060205,"sku":"305124","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SNU-387_20WaKo_20P1_2010x01_20270924_ch00_1920x1920_85af81f1-dc45-4957-8f44-d3df05020373.jpg?v=1769069233"},{"product_id":"snu-398-cell-bhc11101450","title":"SNU-398 cell","description":"The SNU-398 cell line is derived from a hepatocellular carcinoma (HCC) of a human adult. This cell line is extensively used in liver cancer research to study the molecular mechanisms underlying hepatocarcinogenesis, tumor progression, and the development of therapeutic strategies. Hepatocellular carcinoma is a prevalent and deadly form of liver cancer, and SNU-398 cells provide a relevant model for investigating the genetic and epigenetic changes associated with this disease.\nSNU-398 cells exhibit an epithelial morphology and express markers characteristic of liver cancer, such as alpha-fetoprotein (AFP) and cytokeratins. They harbor genetic mutations and alterations typical of HCC, including mutations in the TP53 gene, which is commonly associated with many cancers. Researchers utilize SNU-398 cells to explore various signaling pathways involved in liver cancer, such as the Wnt\/β-catenin, PI3K\/Akt, and MAPK pathways. These cells are also employed in drug screening assays to evaluate the efficacy of chemotherapeutic agents and targeted therapies, as well as in studies investigating resistance mechanisms to conventional treatments. The SNU-398 cell line's importance in hepatocellular carcinoma research lies in its ability to model liver cancer biology and to contribute to the development of more effective therapies for liver cancer patients.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101450\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950225092973,"sku":"305274","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/snu-398_20_282_29_1920x1920_5cd7483f-1bc7-4092-b761-682bf7b06831.jpg?v=1769069234"},{"product_id":"snu-449-cell-bhc11101511","title":"SNU-449 cell","description":"SNU-449 is a human hepatocellular carcinoma (HCC) cell line widely used in research to study liver cancer biology, drug resistance, apoptosis, and novel therapeutic strategies. As hepatocellular carcinoma is one of the most aggressive and common liver malignancies with poor prognosis, cell lines like SNU-449 are critical for understanding the molecular mechanisms underlying cancer progression and drug responses.\nSNU-449 has been particularly useful in studies involving apoptosis and ferroptosis, a regulated form of cell death associated with iron-dependent lipid peroxidation. For example, research has shown that agents like sorafenib, a standard treatment for advanced HCC, and artesunate synergize to induce ferroptosis in SNU-449 cells. This combination exacerbates lipid peroxidation and oxidative stress, leading to extensive cancer cell death. This synergy occurs because artesunate promotes lysosomal ferritin degradation (ferritinophagy), which increases free iron availability, while sorafenib impairs mitochondrial function and depletes glutathione, a critical antioxidant.\nSNU-449 has also been used to explore apoptotic pathways in liver cancer. For example, genistein, a natural isoflavone, induces apoptosis in SNU-449 cells by down-regulating thioredoxin-1 (Trx1), an antioxidant protein that regulates reactive oxygen species (ROS) and inhibits apoptosis. Genistein treatment increases ROS levels and activates apoptosis-related pathways, including caspase-3 activation and DNA fragmentation. These findings highlight SNU-449 as a valuable model for studying both apoptosis and ferroptosis, aiding in the development of targeted therapies for hepatocellular carcinoma.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101511\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950225125741,"sku":"305429","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SNU-449_20WaKo_20P1_2010x01_20141024_ch00_1920x1920_4cc195b5-e5d2-42ee-b7f2-d6a07ee9344f.jpg?v=1769069233"},{"product_id":"snu-878-cell-bhc11101439","title":"SNU-878 cell","description":"The SNU-878 cell line is derived from a human hepatocellular carcinoma (HCC), which is a primary malignancy of the liver. This cell line is widely used in liver cancer research to study the molecular and cellular mechanisms underlying hepatocarcinogenesis, tumor progression, and therapeutic responses. Hepatocellular carcinoma is one of the most common and deadly forms of liver cancer, making cell lines like SNU-878 essential for advancing our understanding of the disease and developing effective treatments.\nSNU-878 cells exhibit an epithelial morphology and express markers typical of liver cancer, such as alpha-fetoprotein (AFP) and hepatocyte-specific antigens. They harbor genetic and epigenetic alterations that are frequently observed in HCC, including mutations in key oncogenes and tumor suppressor genes. Researchers use SNU-878 cells to explore various signaling pathways involved in liver cancer, such as the Wnt\/β-catenin, PI3K\/Akt, and MAPK pathways. These cells are also employed in high-throughput drug screening assays and preclinical testing of chemotherapeutic agents, targeted therapies, and combination treatments. Additionally, SNU-878 cells are utilized to study mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the SNU-878 cell line in hepatocellular carcinoma research highlights its importance in advancing our knowledge of liver cancer biology and in the development of new therapeutic approaches for patients with HCC.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101439\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950225256813,"sku":"305285","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SNU-878_20WaKo_20P1_2010x01_20250914_ch00_1920x1920_b3926acb-e723-4c81-aa51-87b588fa1161.jpg?v=1769069235"},{"product_id":"are-luciferase-reporter-hepg2-cell-line-nrf2-antioxidant-pathway-bhc18200018","title":"ARE Luciferase Reporter HepG2 Cell Line (Nrf2 Antioxidant Pathway)","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table tr:nth-child(even) th{background:#dce8fb}\n.bhp-desc .bhp-spec-table tr:nth-child(even) td{background:#f0f6ff}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-bottom:10px}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444;margin:10px 0 14px}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThe Nrf2 antioxidant response pathway plays an important role in cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eAntioxidant Signaling pathway\u003c\/span\u003e\n\u003cp\u003eThe ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.\u003c\/p\u003e\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Cell Line\u003c\/th\u003e\n\u003ctd\u003eHepG2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Method\u003c\/th\u003e\n\u003ctd\u003eLipofectamine 2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eAqueous solution containing DMSO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplied As\u003c\/th\u003e\n\u003ctd\u003eEach vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHarmonized Tariff Code\u003c\/th\u003e\n\u003ctd\u003e3002-5900\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eQuality Control \u0026amp; Validation\u003c\/h2\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003e✓ Mycoplasma-Tested\u003c\/span\u003e\n\u003cp\u003eThe cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cp\u003eSee data sheet for detailed culturing and assay protocol.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cp style=\"margin:0 0 4px\"\u003e⚠ Avoid freeze\/thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eRegulatory Information\u003c\/h2\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLicense Required\u003c\/strong\u003eYes\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLiving Modified Organism\u003c\/strong\u003eYes\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eLicense Disclosure\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003ePurchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eRelated Products: Cat. #60514, 79533, 60690, 60187, 79796\u003c\/p\u003e\n\u003cp\u003eRequired Accessories: Cat. #79533,60690,60187,79796\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"2 Vials","offer_id":53322313826669,"sku":"60513","price":2825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/60513_graph1.png?v=1778636460"},{"product_id":"crispra-sam-hepg2-cell-line-bhc18200097","title":"CRISPRa (SAM) HepG2 Cell Line","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table tr:nth-child(even) th{background:#dce8fb}\n.bhp-desc .bhp-spec-table tr:nth-child(even) td{background:#f0f6ff}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-bottom:10px}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444;margin:10px 0 14px}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eCRISPR\u003c\/span\u003e\n\u003cp\u003eThis cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene.\u003c\/p\u003e\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Cell Line\u003c\/th\u003e\n\u003ctd\u003eHepG2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Method\u003c\/th\u003e\n\u003ctd\u003eLentivirus, no catalog number,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplied As\u003c\/th\u003e\n\u003ctd\u003eEach vial contains 2 x 10\u003csup\u003e6\u003c\/sup\u003e cells in 1 ml of 10% DMSO.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHarmonized Tariff Code\u003c\/th\u003e\n\u003ctd\u003e3002-5900\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eQuality Control \u0026amp; Validation\u003c\/h2\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003e✓ Mycoplasma-Tested\u003c\/span\u003e\n\u003cp\u003eThis cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cp\u003eSee assay protocol for detailed instructions.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cp style=\"margin:0 0 4px\"\u003e⚠ Avoid freeze\/thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eRegulatory Information\u003c\/h2\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLicense Required\u003c\/strong\u003eYes\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLiving Modified Organism\u003c\/strong\u003eYes\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eLicense Disclosure\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eThe CRISPR\/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights. License Disclosure: Visit bpsbioscience.com\/license for the label license and other key information about this product. Troubleshooting Guide: Visit bpsbioscience.com\/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eRelated Products: Cat. #60187, 78080, 78221, 78193\u003c\/p\u003e\n\u003cp\u003eRequired Accessories: Cat. #60187,78221,79796\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"2 Vials","offer_id":53322313990509,"sku":"78194","price":2500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/78194_1.png?v=1778636457"}],"url":"https:\/\/www.ebiohippo.com\/collections\/liver-cancer-cell-lines.oembed","provider":"BioHippo","version":"1.0","type":"link"}