{"title":"ProFoldin Binding \u0026 Separation","description":"\u003cp\u003eProFoldin binding plates, spin columns and nucleic acid removal tools.\u003c\/p\u003e","products":[{"product_id":"dna-binding-fluorescence-interference-assay-kit-bht16300160","title":"DNA-Binding Fluorescence Interference Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Binding Assay Kit\u003c\/span\u003e\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eDBF100K\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Buffer\u003c\/td\u003e\n\u003ctd\u003e550 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x DNA\u003c\/td\u003e\n\u003ctd\u003e110 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Dye B22\u003c\/td\u003e\n\u003ctd\u003e110 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eDNA binding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eBinding Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eA detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The DNA-Binding Fluorescence Interference Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67794881577325,"sku":"DBF100K","price":375.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DBF100K_fig.jpg?v=1792791975"},{"product_id":"gel-shift-single-strand-dna-binding-assay-kit-bht16300159","title":"Gel Shift Single-strand DNA Binding Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Binding Assay Kit\u003c\/span\u003e\u003cp\u003eSingle-strand DNA-binding protein (SSB) binds single-stranded DNA, or single-stranded regions of double-stranded DNA, during DNA replication, recombination and repair; for example, DNA helicase unwinding creates single-strand DNA. E. coli SSB forms a homotetramer of 19 kDa subunits.\u003c\/p\u003e\n\u003ch2\u003eKit vs Kit Plus\u003c\/h2\u003e\n\u003cp\u003eThe only difference is that GSSB100KE (Kit Plus) also includes 150 µl of 2 mg\/ml E. coli SSB protein; GSSB100K contains the buffer, ssDNA and loading buffer only, for use with your own SSB protein.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eKit (SSB protein not included), 100 assays (GSSB100K)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Plus (SSB protein included), 100 assays (GSSB100KE)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eKit (SSB protein not included), 100 assays (GSSB100K)\u003c\/th\u003e\n\u003cth\u003eKit Plus (SSB protein included), 100 assays (GSSB100KE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Buffer\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x ssDNA (45-mer single-strand DNA)\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4 x Gel loading buffer\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE. coli SSB protein (2 mg\/ml) (also sold separately as E. coli Single-Strand DNA Binding Protein, BHP16300072)\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eSSB is mixed with a 45-mer single-strand DNA and run on a conventional acrylamide gel. SSB-bound DNA migrates more slowly than free DNA, so binding activity shows as a band shift.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eSSB single-strand DNA binding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eSingle-strand DNA-binding protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eBinding Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eGel Electrophoresis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe manufacturer's PDF describes the assay and kit contents; please contact us for the full procedure.\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003eGel Shift Single-strand DNA Binding Assay (PDF, see Documents): Covers catalog # GSSB100K, GSSB100KE.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Gel Shift Single-strand DNA Binding Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Kit (SSB protein not included)","offer_id":67794881839469,"sku":"GSSB100K","price":207.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (SSB protein included)","offer_id":67794884788589,"sku":"GSSB100KE","price":612.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GSSB100KE_fig.jpg?v=1792791971"},{"product_id":"e-coli-single-strand-dna-binding-protein-bhp16300072","title":"E. coli Single-Strand DNA Binding Protein","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA-Binding Protein\u003c\/span\u003e\u003cp\u003eSingle-strand DNA-binding protein (SSB) binds single-stranded DNA, or single-stranded regions of double-stranded DNA, during DNA replication, recombination and repair; for example, DNA helicase unwinding creates single-strand DNA. E. coli SSB forms a homotetramer of 19 kDa subunits.\u003c\/p\u003e\n\u003cp\u003eThis product is wild-type E. coli SSB with an N-terminal His-tag, expressed in a bacterial expression system and purified by column chromatography.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e100 µg – SSB-100EC\u003c\/th\u003e\n\u003ctd\u003e50 µl of 2 mg\/ml E. coli single-strand DNA binding protein (100 µg) in 10 mM Tris-HCl, pH 7.5, 20% glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003eDNA-Binding Proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein Construct\u003c\/th\u003e\n\u003ctd\u003eWild-type E. coli single-strand DNA binding protein with an N-terminal His-tag, expressed in a bacterial expression system and purified by column chromatography\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eN-terminal His-tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eBacterial expression system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003e20.7 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Buffer\u003c\/th\u003e\n\u003ctd\u003e10 mM Tris-HCl, pH 7.5, 20% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eUse 10 mM Tris-HCl buffer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative Names\u003c\/th\u003e\n\u003ctd\u003eSSB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eSSB-100EC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e-20°C or -80°C. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUse With\u003c\/h2\u003e\n\u003cp\u003eThis protein is used in the following ProFoldin assay kit:\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003e\n\u003ca href=\"\/products\/gel-shift-single-strand-dna-binding-assay-kit-bht16300159\"\u003eGel Shift Single-strand DNA Binding Assay Kit\u003c\/a\u003e (BHT16300159) — GSSB100KE already includes this protein; GSSB100K is protein-free and needs this product\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe ProFoldin datasheet “E. coli Single-Strand DNA Binding Protein” (linked under Documents) gives the protein construct, storage and dilution. DNA binding activity is measured with the Gel Shift Single-strand DNA Binding Assay Kit; its ProFoldin protocol “Gel Shift Single-strand DNA Binding Assay” is also linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. This protein is shipped frozen. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 µg","offer_id":67815138034029,"sku":"SSB-100EC","price":433.0,"currency_code":"USD","in_stock":true}]},{"product_id":"96-well-anionic-protein-binding-plates-bhu16300003","title":"96-Well Anionic Protein-Binding Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBinding Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well Anionic Protein-Binding Plates bind negatively charged proteins. Proteins bound to the plate often keep their native conformation and their catalytic or binding activity, so a second molecule that needs the native structure can still recognize the bound protein.\u003c\/p\u003e\n\u003cp\u003eThe plates can be used for ELISA and for studies of protein-protein, protein-peptide, protein-DNA and protein-RNA interactions. The bound second molecule is detected by biochemical, biophysical or immunological methods.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003cp\u003eExample data: binding signal on a DNA-binding plate (left) and a protein-binding plate (right).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eAPP96B\u003c\/td\u003e\n\u003ctd\u003eAPP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaterial\u003c\/th\u003e\n\u003ctd\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinds\u003c\/th\u003e\n\u003ctd\u003eNegatively charged proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eAPP96B, APP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eAdd 200 µl of 0.5-1 mg\/ml protein in a low-salt buffer (10 mM buffer, 10 mM NaCl, 0.01% Tween-20, 1 mM EDTA) to each well and incubate at 4°C overnight, then rinse.\u003c\/li\u003e\n\u003cli\u003eBlock with 300 µl of blocker for 30 min: 1% BSA when the second molecule is a protein, or 1× Nucleic Acid Binding Blocker when it is a nucleic acid.\u003c\/li\u003e\n\u003cli\u003eAdd 200 µl of the second molecule in low-salt buffer and incubate for 1 hour, then rinse.\u003c\/li\u003e\n\u003cli\u003eDetect the bound second molecule, for example by enzyme activity, a fluorescent or radioactive label, or SYBR Green fluorescence (Ex 485 nm \/ Em 535 nm) for nucleic acids.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Protein-binding Plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe Nucleic Acid Binding Blocker used for nucleic acid partners is sold separately.\u003c\/li\u003e\n\u003cli\u003eThe second molecule concentration depends on its binding affinity; about 0.1 mg\/ml is typical for proteins, lower for antibodies, and micromolar for DNA oligos.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923319149,"sku":"APP96B","price":152.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815923941741,"sku":"APP96T","price":152.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNA-binding_plates_640_5e818fe8-74cd-46a5-a396-69911db1e79a.gif?v=1793049625"},{"product_id":"96-well-cationic-protein-binding-plates-bhu16300004","title":"96-Well Cationic Protein-Binding Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBinding Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well Cationic Protein-Binding Plates bind positively charged proteins. Proteins bound to the plate often keep their native conformation and their catalytic or binding activity, so a second molecule that needs the native structure can still recognize the bound protein.\u003c\/p\u003e\n\u003cp\u003eThe plates can be used for ELISA and for studies of protein-protein, protein-peptide, protein-DNA and protein-RNA interactions. The bound second molecule is detected by biochemical, biophysical or immunological methods.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003cp\u003eExample data: binding signal on a DNA-binding plate (left) and a protein-binding plate (right).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eCPP96B\u003c\/td\u003e\n\u003ctd\u003eCPP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaterial\u003c\/th\u003e\n\u003ctd\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinds\u003c\/th\u003e\n\u003ctd\u003ePositively charged proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eCPP96B, CPP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eAdd 200 µl of 0.5-1 mg\/ml protein in a low-salt buffer (10 mM buffer, 10 mM NaCl, 0.01% Tween-20, 1 mM EDTA) to each well and incubate at 4°C overnight, then rinse.\u003c\/li\u003e\n\u003cli\u003eBlock with 300 µl of blocker for 30 min: 1% BSA when the second molecule is a protein, or 1× Nucleic Acid Binding Blocker when it is a nucleic acid.\u003c\/li\u003e\n\u003cli\u003eAdd 200 µl of the second molecule in low-salt buffer and incubate for 1 hour, then rinse.\u003c\/li\u003e\n\u003cli\u003eDetect the bound second molecule, for example by enzyme activity, a fluorescent or radioactive label, or SYBR Green fluorescence (Ex 485 nm \/ Em 535 nm) for nucleic acids.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Protein-binding Plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe Nucleic Acid Binding Blocker used for nucleic acid partners is sold separately.\u003c\/li\u003e\n\u003cli\u003eThe second molecule concentration depends on its binding affinity; about 0.1 mg\/ml is typical for proteins, lower for antibodies, and micromolar for DNA oligos.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923253613,"sku":"CPP96B","price":160.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815924040045,"sku":"CPP96T","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNA-binding_plates_640_4d8f708a-c0de-4dd8-8c58-8f12e25c3f44.gif?v=1793049625"},{"product_id":"micro-phosphate-removal-column-set-bhu16300006","title":"Micro Phosphate Removal Column Set","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSpin Columns\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Micro Phosphate Removal Column Set removes inorganic phosphate from buffer solutions. Phosphate binds to the column resin and stays on the column, while regular buffer salts and non-charged molecules such as sugars and glycerol do not bind and stay in the sample.\u003c\/p\u003e\n\u003cp\u003eSome organic phosphates such as ATP also bind to the resin, but less strongly than inorganic phosphate. Metal chelators such as EDTA interfere with phosphate binding to the resin. Removing phosphate is useful, for example, before phosphatase activity assays.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e20 columns – MPR020\u003c\/th\u003e\n\u003ctd\u003e20 pre-packed spin columns.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003ePre-packed spin columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e20 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Volume\u003c\/th\u003e\n\u003ctd\u003e200 µl per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eResin Binding Capacity\u003c\/th\u003e\n\u003ctd\u003e200 µmol phosphate per ml of bed volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRemoval per Column (0.2 ml sample)\u003c\/th\u003e\n\u003ctd\u003e500 mM to 3 mM; 350 mM to 0.5 mM; 175 mM to 0.2 mM; 10 mM to 0.0003 mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEquipment Needed\u003c\/th\u003e\n\u003ctd\u003eBench-top microcentrifuge and 1.5 ml microcentrifuge tubes as receiver tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eMPR020\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSpin each column briefly to settle the resin, remove the bottom tip and cap, and spin twice at 13,000 rpm for 1 min so the resin is almost dry.\u003c\/li\u003e\n\u003cli\u003eMove the column to a clean 1.5 ml tube, load 200 µl of sample, spin at 1,000 rpm for 1 min and then at 13,000 rpm for 1 min, and keep the eluate.\u003c\/li\u003e\n\u003cli\u003eSpin the eluate at 13,000 rpm for 1 min, or filter it through a 0.22 µm filter, to remove any insoluble material.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Micro Phosphate Removal Column Set” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eAvoid EDTA and other metal chelators in the sample; they interfere with phosphate binding to the resin.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"20 columns","offer_id":67815923384685,"sku":"MPR020","price":310.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MPR020_copy.jpg?v=1793049625"},{"product_id":"96-well-super-high-binding-elisa-plates-bhu16300005","title":"96-Well Super High Binding ELISA Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eELISA Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well Super High Binding ELISA Plates are designed to give strong ELISA signals. Strong protein binding to the plate surface increases ELISA sensitivity.\u003c\/p\u003e\n\u003cp\u003eIn the indirect format, the antigen is coated directly onto the plate, the plate is blocked, an antibody against the antigen is added, and the bound antibody is detected with an enzyme-linked secondary antibody. When two antibodies (A and B) against the antigen are available, antibody A can be coated first to capture the antigen, which is then detected with antibody B and an enzyme-linked antibody; this antibody pre-coating may further increase sensitivity.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eELISA96B\u003c\/td\u003e\n\u003ctd\u003eELISA96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTypical Coating Volume\u003c\/th\u003e\n\u003ctd\u003e100 µl per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTypical Wash \/ Block Volume\u003c\/th\u003e\n\u003ctd\u003e360 µl per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eELISA96B, ELISA96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eCoat each well with 100 µl of antigen (2-fold dilution series in PBS with 0.02% sodium azide; a typical top concentration is 1 µg\/ml) and rotate at 4°C overnight.\u003c\/li\u003e\n\u003cli\u003eWash 4 times with 360 µl of PBS with 0.01% Tween-20, then block with 360 µl of PBS with 0.02% sodium azide and 3% fetal bovine serum for 2 hours at room temperature.\u003c\/li\u003e\n\u003cli\u003eWash, add 100 µl of primary antibody diluted in PBS with 0.02% sodium azide and 0.1% fetal bovine serum, and incubate with rotation for 1 hour at room temperature.\u003c\/li\u003e\n\u003cli\u003eWash, add 100 µl of the secondary antibody for 1 hour, wash again, add 200 µl of detection solution and read absorbance or fluorescence.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “96-well Super High Binding ELISA Plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFor antibody pre-coating, coat 100 µl of 1 µg\/ml antibody A at 4°C overnight or at 37°C for 2 hours, block, and then continue from the antigen step without a second blocking step.\u003c\/li\u003e\n\u003cli\u003eIn the antibody pre-coating format, antibody B should come from a different host species than antibody A, or be directly labeled, so that the enzyme-linked secondary antibody does not bind the capture antibody.\u003c\/li\u003e\n\u003cli\u003eSodium azide inhibits horseradish peroxidase (HRP); leave it out of buffers used with HRP-conjugated antibodies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923351917,"sku":"ELISA96B","price":160.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815923876205,"sku":"ELISA96T","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ELISA96_copy.jpg?v=1793049625"},{"product_id":"96-well-dna-binding-plates-bhu16300002","title":"96-Well DNA-Binding Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBinding Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well DNA-Binding Plates are polystyrene plates coated with positive charges that bind nucleic acids (DNA or RNA). They are used to study specific interactions between a bound nucleic acid and a second molecule, either another nucleic acid or a protein.\u003c\/p\u003e\n\u003cp\u003eThe nucleic acid is first bound to the plate through charge-charge interactions. After blocking, the plate is incubated with the second molecule, and any second molecule that binds to the immobilized nucleic acid is detected by biochemical or biophysical methods. The 96-well format can also be used to screen libraries of nucleic acids or proteins for binding to the immobilized nucleic acid.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003cp\u003eExample data: binding signal on a DNA-binding plate (left) and a protein-binding plate (right).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNBP96B\u003c\/td\u003e\n\u003ctd\u003eNBP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaterial\u003c\/th\u003e\n\u003ctd\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSurface\u003c\/th\u003e\n\u003ctd\u003ePositively charged; binds DNA and RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA Binding Capacity\u003c\/th\u003e\n\u003ctd\u003eAbout 1.1 ng\/mm² (measured with a 1,000-nt DNA oligo at 2 µg\/µl)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStability of Bound DNA\u003c\/th\u003e\n\u003ctd\u003eStable below 200 mM salt, and with non-ionic detergents such as Triton X-100 and Brij-35 below their CMC; SDS interferes with DNA binding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNBP96B, NBP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eAdd 200 µl of 10 µg\/ml DNA in a low-salt buffer (10 mM buffer, 10 mM NaCl, 0.01% Tween-20, 1 mM EDTA) to each well and incubate at 4°C for 2 hours to overnight; overnight binding gives more complete binding.\u003c\/li\u003e\n\u003cli\u003eRinse, then block with 300 µl of blocker for 30 min: 1% BSA when the second molecule is a protein, or 1× Nucleic Acid Binding Blocker when it is a nucleic acid.\u003c\/li\u003e\n\u003cli\u003eAdd 200 µl of the second molecule in low-salt buffer and incubate for 1 hour, then rinse.\u003c\/li\u003e\n\u003cli\u003eDetect the bound second molecule, for example by enzyme activity, a fluorescent or radioactive label, or SYBR Green fluorescence (Ex 485 nm \/ Em 535 nm) for nucleic acids.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “96-well DNA–binding plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe Nucleic Acid Binding Blocker used for nucleic acid partners is sold separately.\u003c\/li\u003e\n\u003cli\u003eThe second molecule concentration depends on its binding affinity; about 0.1 mg\/ml is typical for proteins, lower for antibodies, and micromolar for DNA oligos.\u003c\/li\u003e\n\u003cli\u003eDetergents may affect protein function in the binding step.\u003c\/li\u003e\n\u003cli\u003eWhen the second molecule is a nucleic acid detected with SYBR Green, include wells with the bound DNA alone as background, because the dye also stains the immobilized DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923417453,"sku":"NBP96B","price":143.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815923908973,"sku":"NBP96T","price":143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNA-binding_plates_640.gif?v=1793049625"},{"product_id":"protein-and-dna-removal-spin-columns-bhu16300001","title":"Protein and DNA Removal Spin-Columns","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSpin Columns\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eProtein and DNA Removal Spin-Columns separate small molecules and liposomes from proteins, DNA and RNA. Proteins, nucleic acids and drugs bound to them stay on the column, while small polar molecules or liposomes pass into the eluate.\u003c\/p\u003e\n\u003cp\u003eTypical uses include removing DNA, RNA or proteins from biological samples before HPLC or other analysis, separating free and liposome-encapsulated drugs from protein-bound or DNA-bound drugs, and separating free ligands from receptor-bound ligands. Binding to the resin is mainly through charge-charge interactions; organic and inorganic phosphates may also bind to the column.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e20 columns – PNR020\u003c\/th\u003e\n\u003ctd\u003e20 pre-packed spin columns in 50% ethanol.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003ePre-packed spin columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e20 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Volume\u003c\/th\u003e\n\u003ctd\u003e0.2 ml per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinding Capacity\u003c\/th\u003e\n\u003ctd\u003eMore than 100 µg of protein or 20 µg of DNA per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColumn Storage Solution\u003c\/th\u003e\n\u003ctd\u003e50% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEquipment Needed\u003c\/th\u003e\n\u003ctd\u003eBench-top microcentrifuge and 1.5 ml microcentrifuge tubes as receiver tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003ePNR020\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSpin each column briefly to settle the resin, remove the bottom tip and cap, and spin at 13,000 rpm for 1 min to remove the storage solution.\u003c\/li\u003e\n\u003cli\u003eWash with 200 µl of water (13,000 rpm, 1 min) and move the column to a clean 1.5 ml tube.\u003c\/li\u003e\n\u003cli\u003eLoad 200 µl of sample (in a buffer containing 100 mM NaCl), spin at 1,000 rpm for 1 min and then at 13,000 rpm for 1 min, and keep the eluate.\u003c\/li\u003e\n\u003cli\u003eSpin the eluate at 13,000 rpm for 1 min, or filter it through a 0.22 µm filter, to remove any insoluble material.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Protein and DNA Removal Spin-columns” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIncluding 100 mM NaCl in the sample is not needed to remove protein or DNA, but it helps keep small charged molecules from binding to the column.\u003c\/li\u003e\n\u003cli\u003eLarger samples can be processed by repeating the loading step until the column binding capacity is reached.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"20 columns","offer_id":67815923450221,"sku":"PNR020","price":311.0,"currency_code":"USD","in_stock":true}]},{"product_id":"nucleic-acid-removal-kit-bht16300172","title":"Nucleic Acid Removal Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eProtein Purification\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNucleic acids interfere with many steps of protein work, including cell lysis, chromatographic purification, protein quantification, activity assays and crystallization, especially when the protein of interest binds DNA or RNA. The Nucleic Acid Removal Kit removes nucleic acids from a protein solution or cell lysate to below 250 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eNAR Reagent 1 and Reagent 2 are ionic polymers that precipitate nucleic acids, which are then removed by centrifugation. The final solution contains 0.45 M NaCl, and the reagents are easily removed during subsequent protein purification.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e22 ml + 22 ml (for 200 ml sample) – NAR911\u003c\/th\u003e\n\u003ctd\u003e22 ml NAR Reagent 1 and 22 ml NAR Reagent 2, enough to treat 200 ml of cell lysate or protein solution\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponents\u003c\/th\u003e\n\u003ctd\u003e22 ml NAR Reagent 1, 22 ml NAR Reagent 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCapacity\u003c\/th\u003e\n\u003ctd\u003e200 ml of cell lysate or protein solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eResidual nucleic acid\u003c\/th\u003e\n\u003ctd\u003eBelow 250 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFinal salt\u003c\/th\u003e\n\u003ctd\u003e0.45 M NaCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNAR911\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eKeep all solutions at 0–4°C. Mix one volume of NAR Reagent 1 with 9 volumes of cell suspension (then lyse) or protein solution (then incubate on ice for 10 min).\u003c\/li\u003e\n\u003cli\u003eRemove insoluble material or precipitate by centrifugation at 6000 x g for 20 min at 4°C.\u003c\/li\u003e\n\u003cli\u003eAdd one volume of NAR Reagent 2, incubate on ice for 5 min and remove the precipitate by centrifugation at 6000 x g for 20 min at 4°C.\u003c\/li\u003e\n\u003cli\u003eContinue with protein purification; for ion exchange chromatography, dialyze against a low-salt buffer first.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe ProFoldin protocol “Nucleic Acid Removal” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"22 ml + 22 ml (for 200 ml sample)","offer_id":67828319486317,"sku":"NAR911","price":195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/web.gif?v=1793129658"},{"product_id":"nucleic-acid-binding-blocker-bht16300175","title":"Nucleic Acid Binding Blocker","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBlocking Reagents\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Nucleic Acid Binding Blocker blocks non-specific binding on ProFoldin 96-well DNA-binding and protein-binding plates when the second molecule in a binding experiment is a nucleic acid. It is supplied as a 200 x stock that is diluted 200-fold in low-salt buffer before use.\u003c\/p\u003e\n\u003cp\u003eIn the plate protocols, the wells are blocked with 1 x blocker in low-salt buffer after the first molecule has been bound, and before the nucleic acid partner is added.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e0.5 ml (200 x) – NBP96N\u003c\/th\u003e\n\u003ctd\u003e0.5 ml of 200 x concentrated blocker\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e200 x stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003ctd\u003e0.5 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003e200-fold in low-salt buffer before use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUse with\u003c\/th\u003e\n\u003ctd\u003e96-Well DNA-Binding Plates and Protein-Binding Plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNBP96N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStable at room temperature; store at 4°C or below.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe ProFoldin protocols “96-well DNA–binding plates” and “Protein-binding Plates” are linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eCatalog # NBP96N is this blocker solution. Catalog #s NBP96B (black) and NBP96T (transparent) are the 96-well DNA-binding plates it is used with; it is also used with the 96-well protein-binding plates (APP96B\/T, CPP96B\/T).\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"0.5 ml (200 x)","offer_id":67828368310637,"sku":"NBP96N","price":120.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.ebiohippo.com\/collections\/profoldin-binding-separation.oembed","provider":"BioHippo","version":"1.0","type":"link"}