{"title":"Bioanalytical Method Development","description":null,"products":[{"product_id":"5-azido-5-deoxythymidine-bhb21200088","title":"5′-Azido-5′-deoxythymidine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-Azido-5'-deoxythymidine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Crystalline powder, purity ≥ 98.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5'-Azido-5'-deoxythymidine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C10H13N5O4; 267.24 g\/mol; ≥ 98.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5'-Azido-5'-deoxythymidine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-Azido-5'-deoxythymidine is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249725956461,"sku":"B-02-10MG","price":164.8,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249731920237,"sku":"B-02-25MG","price":289.8,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249731953005,"sku":"B-02-50MG","price":403.5,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249731985773,"sku":"B-02-100MG","price":562.5,"currency_code":"USD","in_stock":true},{"title":"250 mg","offer_id":53249732018541,"sku":"B-02-250MG","price":772.8,"currency_code":"USD","in_stock":true},{"title":"500 mg","offer_id":53249732051309,"sku":"B-02-500MG","price":841.0,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53249732084077,"sku":"B-02-1G","price":966.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_d2fc3e3b40b542da8c994570d55f513c_mv2.png?v=1776855582"},{"product_id":"5-3-tips-5-methyluridine-bhb21200090","title":"5′,3′-TIPS 5-methyluridine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5',3'-TIPS 5-methyluridine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Crystalline powder, purity ≥ 98.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 3',5'-O-(1,1,3,3-Tetraisopropyl-1,3-disiloxanediyl)-5-methyluridine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C22H40N2O7Si2; 500.73 g\/mol; ≥ 98.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 3',5'-O-(1,1,3,3-Tetraisopropyl-1,3-disiloxanediyl)-5-methyluridine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5',3'-TIPS 5-methyluridine is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"1 g","offer_id":53249726906733,"sku":"B-04-1G","price":551.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_94309859a3d24cc3906ba55130b68ff8_mv2.png?v=1776855585"},{"product_id":"5-iodo-n4-benzoyl-2-deoxycytidine-bhb21200093","title":"5′-Iodo-N4-benzoyl-2′-deoxycytidine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-iodo-N4-Benzoyl-2'-deoxycytidine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Powder to crystalline powder, purity ≥ 95.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5'-Iodo-N4-benzoyl-2'-deoxycytidine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C16H16IN3O4; 441.23 g\/mol; ≥ 95.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Powder to crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5'-Iodo-N4-benzoyl-2'-deoxycytidine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-iodo-N4-Benzoyl-2'-deoxycytidine is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249727267181,"sku":"B-07-10MG","price":153.5,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249730543981,"sku":"B-07-25MG","price":267.1,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249730576749,"sku":"B-07-50MG","price":375.0,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249730609517,"sku":"B-07-100MG","price":528.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_9446fe9327904c7fbd367bc05caa22dd_mv2.png?v=1776855584"},{"product_id":"5-o-dmt-5-methyluridine-bhb21200089","title":"5′-O-DMT-5-methyluridine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-O-DMT-5-methyluridine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Crystalline powder, purity ≥ 95.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5′-O-(4,4′-Dimethoxytrityl)-5-methyluridine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C31H32N2O8; 560.59 g\/mol; ≥ 95.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5′-O-(4,4′-Dimethoxytrityl)-5-methyluridine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-O-DMT-5-methyluridine is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249727398253,"sku":"B-03-10MG","price":62.5,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249734312301,"sku":"B-03-25MG","price":109.1,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249734345069,"sku":"B-03-50MG","price":153.5,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249734377837,"sku":"B-03-100MG","price":216.0,"currency_code":"USD","in_stock":true},{"title":"250 mg","offer_id":53249734410605,"sku":"B-03-250MG","price":358.0,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53249734443373,"sku":"B-03-1G","price":716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_1368f14199bf43aca30c590fae8a09de_mv2.png?v=1776855584"},{"product_id":"5-deoxy-5-iodothymidine-bhb21200087","title":"5′-Deoxy-5′-iodothymidine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-deoxy-5'-iodothymidine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Crystalline powder, purity ≥ 95.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5'-Deoxy-5'-iodothymidine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C10H13IN2O4; 352.13 g\/mol; ≥ 95.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5'-Deoxy-5'-iodothymidine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-deoxy-5'-iodothymidine is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249727496557,"sku":"B-01-10MG","price":62.5,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249731395949,"sku":"B-01-25MG","price":125.0,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249731428717,"sku":"B-01-50MG","price":193.2,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249731461485,"sku":"B-01-100MG","price":284.1,"currency_code":"USD","in_stock":true},{"title":"250 mg","offer_id":53249731494253,"sku":"B-01-250MG","price":517.1,"currency_code":"USD","in_stock":true},{"title":"500 mg","offer_id":53249731527021,"sku":"B-01-500MG","price":755.7,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53249731559789,"sku":"B-01-1G","price":1091.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_2e0c8dd2828743d8aa9050c34d045bb0_mv2.png?v=1776855584"},{"product_id":"5-iodo-n2-isobutyryl-2-deoxyguanosine-bhb21200092","title":"5′-Iodo-N2-isobutyryl-2′-deoxyguanosine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-iodo-N2-isobutyryl-2′-deoxyguanosine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Foam, purity ≥ 95.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5'-Iodo-N2-isobutyryl-2′-deoxyguanosine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C14H18IN5O4; 447.23 g\/mol; ≥ 95.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm \/ Solubility:\u003c\/strong\u003e Foam; .\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5'-Iodo-N2-isobutyryl-2′-deoxyguanosine.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOrigin:\u003c\/strong\u003e Synthetic synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-iodo-N2-isobutyryl-2′-deoxyguanosine is a CoA thioester derivative involved in intermediary metabolism.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAcyl-CoA metabolism increasingly linked to histone acylation marks and epigenetic regulation in cancer and metabolic disease research.\u003c\/li\u003e\n\u003cli\u003eGrowing interest in short-chain fatty acid CoA thioesters as mediators of gut microbiome–host metabolic crosstalk.\u003c\/li\u003e\n\u003cli\u003eCoA-dependent enzymes investigated as drug targets in infectious disease and neurometabolic disorder research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme kinetics assays — direct substrate for acyltransferases, thiolases, and dehydrogenases.\u003c\/li\u003e\n\u003cli\u003eMetabolic flux analysis — isotope-labeled variants available for stable-isotope tracing.\u003c\/li\u003e\n\u003cli\u003eIn vitro pathway reconstitution for fatty acid β-oxidation, TCA cycle, or polyketide biosynthesis.\u003c\/li\u003e\n\u003cli\u003eBiochemical characterization of CoA-binding proteins by activity assays or binding measurements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCoA thioesters hydrolyze at neutral–alkaline pH; prepare working solutions fresh and keep on ice.\u003c\/li\u003e\n\u003cli\u003eDifferent salt forms share the same core structure — normalize concentrations using the free-acid MW when comparing across forms.\u003c\/li\u003e\n\u003cli\u003eThiol oxidation may occur upon air exposure; use under inert atmosphere or with reducing agents where appropriate.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249728086381,"sku":"B-06-10MG","price":170.5,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249730314605,"sku":"B-06-25MG","price":295.5,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249730347373,"sku":"B-06-50MG","price":409.1,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249730380141,"sku":"B-06-100MG","price":568.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_df81c724048841ddbaf141ebd1191446_mv2.png?v=1776855585"},{"product_id":"5-o-dmt-thymidine-bhb21200091","title":"5′-O-DMT-Thymidine","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-O-DMT-Thymidine is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Crystalline powder, purity ≥ 98.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5′-O-(4,4′-Dimethoxytrityl)thymidine, 5′-O-[bis(4-methoxyphenyl)phenylmethyl]thymidine, 5′-O-[α,α-bis(p-methoxyphenyl)benzyl]thymidine.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C31H32N2O7; 544.60 g\/mol; ≥ 98.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5′-O-(4,4′-Dimethoxytrityl)thymidine, 5′-O-[bis(4-methoxyphenyl)phenylmethyl]thymidine, 5′-O-[α,α-bis(p-methoxyphenyl)benzyl]thymidine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-O-DMT-Thymidine is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249728217453,"sku":"B-05-10MG","price":68.2,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249733296493,"sku":"B-05-25MG","price":136.4,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249733329261,"sku":"B-05-50MG","price":210.3,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249733362029,"sku":"B-05-100MG","price":312.5,"currency_code":"USD","in_stock":true},{"title":"250 mg","offer_id":53249733394797,"sku":"B-05-250MG","price":573.9,"currency_code":"USD","in_stock":true},{"title":"500 mg","offer_id":53249733427565,"sku":"B-05-500MG","price":829.6,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53249733460333,"sku":"B-05-1G","price":1159.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_08d26e7b5eff42dbab9e3d3e0dd3f540_mv2.png?v=1776855584"},{"product_id":"5-iodo-2-5-dideoxy-thymidine-3-ce-phosphoramidite-bhb21200094","title":"5′-Iodo-2′,5′-dideoxythymidine 3′-CE phosphoramidite","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e5'-iodo-2',5'-dideoxy-thymidine 3'-CE phosphoramidite is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Crystalline powder, purity ≥ 98.0%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e 5'-Iodo-2'5'-dideoxy-thymidine 3'-CE phosphoramidite.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C19H30IN4O5P; 552.35 g\/mol; ≥ 98.0%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Crystalline powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e 5'-Iodo-2'5'-dideoxy-thymidine 3'-CE phosphoramidite.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003e5'-iodo-2',5'-dideoxy-thymidine 3'-CE phosphoramidite is a synthetic nucleoside analog used in oligonucleotide chemistry and nucleic acid research.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRising demand for modified nucleosides in mRNA and antisense oligonucleotide therapeutics development.\u003c\/li\u003e\n\u003cli\u003e5'-Halogenated derivatives used as mechanism-based probes for structural and enzymological studies.\u003c\/li\u003e\n\u003cli\u003ePhosphoramidite nucleosides central to automated solid-phase DNA\/RNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuilding blocks for chemical oligonucleotide synthesis (solid-phase, automated).\u003c\/li\u003e\n\u003cli\u003eSubstrate\/inhibitor characterization for nucleoside kinases, polymerases, and nucleotidases.\u003c\/li\u003e\n\u003cli\u003eClick-chemistry probes and bioconjugation via azide or iodo functional handles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAzide-bearing analogs require appropriate safety precautions.\u003c\/li\u003e\n\u003cli\u003eDMT-protected derivatives are moisture-sensitive; handle under dry conditions.\u003c\/li\u003e\n\u003cli\u003eVerify protecting group compatibility with your deprotection protocol before use.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"100 mg","offer_id":53249728315757,"sku":"C-01-100MG","price":204.6,"currency_code":"USD","in_stock":true},{"title":"250 mg","offer_id":53249734639981,"sku":"C-01-250MG","price":454.6,"currency_code":"USD","in_stock":true},{"title":"500 mg","offer_id":53249734672749,"sku":"C-01-500MG","price":727.3,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53249734705517,"sku":"C-01-1G","price":1170.5,"currency_code":"USD","in_stock":true},{"title":"2 g","offer_id":53249734738285,"sku":"C-01-2G","price":1875.0,"currency_code":"USD","in_stock":true},{"title":"5 g","offer_id":53249734771053,"sku":"C-01-5G","price":3937.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fc74bc_1e3ae8ee36954ddd88440049a53b65ca_mv2.png?v=1776855584"},{"product_id":"denarase-elisa-kit-the-one-for-all-serratia-marcescens-elisa-kit-bhe16500002","title":"DENARASE® ELISA kit The One-for-all Serratia marcescens ELISA Kit","description":"\u003cstyle\u003e\n  .bhp-desc{font-size:16px;color:#1b2f42;line-height:1.75;max-width:860px}\n  .bhp-desc h1{font-size:26px;font-weight:700;color:#b7195d;margin:0 0 20px}\n  .bhp-desc h2{font-size:20px;font-weight:700;color:#b7195d;margin:28px 0 10px}\n  .bhp-desc h3{font-size:18px;font-weight:700;color:#1a5c58;margin:24px 0 8px}\n  .bhp-desc p{margin:0 0 14px}\n  .bhp-desc ul{margin:10px 0 16px 22px;padding:0}\n  .bhp-desc ul li{margin-bottom:10px}\n  .bhp-desc sup{font-size:.72em;vertical-align:super}\n  .bhp-desc em{font-style:italic}\n  .bhp-desc strong{font-weight:700;color:#1b2f42}\n  .bhp-desc hr{border:none;border-top:1px solid #e2e6ea;margin:28px 0}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch1\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e ELISA Kit — The One-for-all \u003cem\u003eSerratia marcescens\u003c\/em\u003e Endonuclease Detection Kit\u003c\/h1\u003e\n\u003ch3\u003eAbout the DENARASE\u003csup\u003e®\u003c\/sup\u003e ELISA Kit\u003c\/h3\u003e\n\u003cp\u003eThe DENARASE\u003csup\u003e®\u003c\/sup\u003e ELISA Kit is a sandwich ELISA for quantitative in vitro measurement of residual \u003cem\u003eSerratia marcescens\u003c\/em\u003e endonuclease in process-related samples. It detects DENARASE\u003csup\u003e®\u003c\/sup\u003e, DENARASE\u003csup\u003e®\u003c\/sup\u003e High Salt, and Benzonase\u003csup\u003e®\u003c\/sup\u003e — providing a unified assay solution for monitoring any \u003cem\u003eS. marcescens\u003c\/em\u003e endonuclease across your downstream bioprocessing workflow.\u003c\/p\u003e\n\u003cp\u003eIntended for research and manufacturing use (process development and quality control). \u003cstrong\u003eNot for diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eDetection Principle\u003c\/h3\u003e\n\u003cp\u003eSandwich ELISA. Samples are incubated in 96-well plates pre-coated with a specific monoclonal capture antibody. Bound endonuclease is detected using a biotin-conjugated monoclonal detector antibody, streptavidin-HRP conjugate, and TMB colorimetric substrate. Absorbance read at OD450.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eKey Performance Parameters\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimit of Detection (LOD):\u003c\/strong\u003e 4 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimit of Quantification (LOQ):\u003c\/strong\u003e 12 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWorking Range:\u003c\/strong\u003e ~32–1,000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e ~130 minutes total incubation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCV (OD450 Assay Control):\u003c\/strong\u003e ≤ 15%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapacity:\u003c\/strong\u003e 480 measurements per kit (5 × 96-well plates)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e15–50× more sensitive\u003c\/strong\u003e than competitor ELISA kits using polyclonal antibodies\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e50% shorter assay time\u003c\/strong\u003e versus competitor kits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e5 × Microtest plates\u003c\/strong\u003e (96-well, pre-coated with monoclonal capture antibody)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing \u0026amp; Dilution Buffer (10×)\u003c\/strong\u003e — 3 × 100 mL + 1 × 20 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDENARASE\u003csup\u003e®\u003c\/sup\u003e Standard (10 µg\/mL)\u003c\/strong\u003e — 1 × 0.1 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetector Antibody (100×)\u003c\/strong\u003e — Biotin-conjugated monoclonal antibody, 1 × 0.75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Conjugate (100×)\u003c\/strong\u003e — Streptavidin-HRP, 1 × 0.75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSubstrate Solution\u003c\/strong\u003e — TMB One, ready to use, 1 × 75 mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStop Solution\u003c\/strong\u003e — 0.5 M sulfuric acid, 1 × 75 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eStorage \u0026amp; Stability\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStore at \u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003eShelf life: \u003cstrong\u003e18 months\u003c\/strong\u003e from production under proper storage conditions\u003c\/li\u003e\n\u003cli\u003eShipping temperature may deviate from storage temperature without affecting product quality\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003ciframe title=\"DENARASE® ELISA Kit for Quantification of Residual 𝘚𝘦𝘳𝘳𝘢𝘵𝘪𝘢 𝘮𝘢𝘳𝘤𝘦𝘴𝘤𝘦𝘯𝘴  Endonucleases\" src=\"https:\/\/www.youtube.com\/embed\/tswMimFgAcc\" height=\"759\" width=\"1349\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e","brand":"c-LEcta GmbH","offers":[{"title":"5 pre-coated 96-well microtiter plates (480 measurements)","offer_id":53276380332397,"sku":"41901-1","price":4195.0,"currency_code":"USD","in_stock":true},{"title":"500mL Washing and dilution buffer (10x)","offer_id":53285362467181,"sku":"41901-2","price":78.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DENARASEELISAKit.webp?v=1777658742"},{"product_id":"hieff-bst-plus-dna-polymerase-bhz20800084","title":"Hieff™ Bst Plus DNA Polymerase","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eHieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cspan\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul style=\"text-align: left;\"\u003e\n\u003cli\u003eThermophilic DNA polymerase with strong strand displacement activity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul style=\"text-align: left;\"\u003e\n\u003cli\u003eIsothermal DNA amplification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003ctd\u003eBst DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeat Inactivation\u003c\/td\u003e\n\u003ctd\u003eIncubation at 85°C for 5 min.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution Components\u003c\/td\u003e\n\u003ctd\u003e10 mmol\/L Tris-HCl, 50 mmol\/L KCl, 0.1 mmol\/L EDTA, 1 mmol\/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eActivity Definition\u003c\/td\u003e\n\u003ctd\u003e1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e14402ES92 (8,000 U)\u003c\/td\u003e\n\u003ctd\u003e14402ES97 (40,000 U)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14402-A\u003c\/td\u003e\n\u003ctd\u003eHieff™ Bst Plus DNA Polymerase (40 U\/μL)\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14402-B\u003c\/td\u003e\n\u003ctd\u003e10 ×Hieff™ Bst Plus DNA Polymerase Buffer\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e3×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14402-C\u003c\/td\u003e\n\u003ctd\u003e100 mmol\/L MgSO4\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e3×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eHigh Sensitivity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg alt=\"Figure 1: Sensitivity Test\" src=\"https:\/\/seas.ysbuy.com\/file\/384681\/131a-167524194263128440.png\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1: Sensitivity Test\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan\u003eRed: 50 copies\/T, response less than 15 minutes\u003cbr\u003eFigure 1: The copy numbers of 50 copies\/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies\/T performed better, and the detection rate reached 100%.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003cspan\u003eStability\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg alt=\"Figure 2.Stability testing at the same temperature\" src=\"https:\/\/seas.ysbuy.com\/file\/93756\/3580-167524288433759822.jpg\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 2.Stability testing at the same temperature\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan\u003eRed: 37℃ 0 day  Blue: 37℃ 1 day  Green: 37℃ 3 days  Purple: 37℃ 5 days\u003cbr\u003e0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan\u003e\u003cimg alt=\"Figure 3. -20℃ repeated freeze-thaw stability test\" src=\"https:\/\/seas.ysbuy.com\/file\/384681\/95bb-167524291024348489.png\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 3. -20℃ repeated freeze-thaw stability test\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cspan\u003eRed: Repeat freeze-thaw 0 times\u003cbr\u003eBlue: Repeat freeze-thaw 5 times\u003cbr\u003eGreen: Repeat freeze-thaw 10 times\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg alt=\"Figure 4. 48-well NTC-specific assay\" src=\"https:\/\/seas.ysbuy.com\/file\/713959\/894b-167524318728957537.png\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003cspan\u003eFigure 4. 48-well NTC-specific assay\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" style=\"text-align: left;\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003eThe product should be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"8000 U","offer_id":53294147797357,"sku":"14402ES92","price":95.0,"currency_code":"USD","in_stock":true},{"title":"40000 U","offer_id":53294158283117,"sku":"14402ES97","price":435.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/131a-167524194263128440.png?v=1778101065"},{"product_id":"cleascrip-t7-rna-polymerase-low-dsrna-250-u-mu-l-bhz20800007","title":"CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U\/μL)","description":"\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 KU","offer_id":53294147895661,"sku":"10628ES10","price":180.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294158020973,"sku":"10628ES60","price":1525.0,"currency_code":"USD","in_stock":true},{"title":"250 KU","offer_id":53294158053741,"sku":"10628ES72","price":3430.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/2_1024x1024_1024x1024_4b20057d-4f93-4241-8dc4-e4e50a7345c8.png?v=1778101061"},{"product_id":"fast-pngase-f-bhz20800012","title":"Fast PNGase F","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003ePNGase F is an amidohydrolase that can cleave high-mannose, hybrid, and complex oligosaccharides linked by asparagine, specifically removing N-linked glycans. However, the conventional PNGase F enzymatic reaction often takes several hours to release antibody N-glycans. Due to the preferential release of glycans, incomplete deglycosylation might result in biased outcomes, with the obtained glycan distribution failing to represent the true composition of therapeutic antibodies. Therefore, rapidly obtaining an accurate N-glycan profile is crucial for glycosylation monitoring during the production of antibodies and antibody-fusion proteins used in biotherapeutics.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eYeasen Fast PNGase F\u003cspan\u003e（\u003c\/span\u003eGlycerol-free\u003cspan\u003e）\u003c\/span\u003eis an optimized recombinant reagent capable of thoroughly and rapidly deglycosylating antibodies, immunoglobulins, fusion proteins, and other glycoproteins within minutes. This enzyme can swiftly and non-selectively remove all N-glycans, enabling direct downstream chromatography or mass spectrometry analysis. Yeasen Fast PNGase F\u003cspan\u003e（\u003c\/span\u003eGlycerol-free\u003cspan\u003e）\u003c\/span\u003e simplifies the experimental procedure while reducing experimental time without compromising sensitivity and reproducibility.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eFast\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003eComplete and rapid deglycosylation in a few minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHigh purity—No protease, glycosidase contamination, purity ≥95%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNon-selective\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003eQuickly and without preference remove all N-glycans.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGood compatibility\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003eDirectly used for downstream chromatography or MS spectrometry analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eGlycan Sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGlycoprotein Analysis\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRecombinant Glycoprotein Expression\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eEnglish synonym\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eFast PNGase F\u003cspan\u003e（\u003c\/span\u003eGlycerol-free\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eYeast recombinant expression\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eStorage buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20406ES20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20406ES50\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20406-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eFast PNGase F\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"cen\u0026lt;p\u0026gt;\u0026lt;strong\u0026gt;Glycerol Content:\u0026lt;\/strong\u0026gt; Contains Glycerol\u0026lt;\/p\u0026gt;\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 T","offer_id":53294148026733,"sku":"20406ES20","price":180.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53294156644717,"sku":"20406ES50","price":345.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20406.webp?v=1778483531"},{"product_id":"endo-h-bhz20800010","title":"Endo H","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eEndo H is a recombinant glycosidase that can cleave the core structure of high-mannose and some hybrid oligosaccharides in N-glycoproteins, removing the N-linked high-mannose from glycoproteins. Endo H is cloned from the corrugated actinomycete (Streptomyces plicatus) and recombinantly expressed in yeast. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eHigh purity—No protease, glycosidase contamination, purity ≥95%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHigh Activity\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003e 1,000,000 U\/mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGood compatibility\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e—\u003c\/span\u003eCan be used denaturing or no-denaturing conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eGlycan Sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProteomics\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGlycoprotein Analysis\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRecombinant Glycoprotein Expression\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\" style=\"width: 100%; height: 331.242px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 55.207px;\"\u003e\n\u003ctd valign=\"center\" style=\"width: 14.1276%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003eEnglish synonym\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 83.468%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003eEndo H\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6016px;\"\u003e\n\u003ctd valign=\"center\" style=\"width: 14.1276%; height: 35.6016px;\"\u003e\n\u003cp\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 83.468%; height: 35.6016px;\"\u003e\n\u003cp\u003e\u003cspan\u003eYeast recombinant expression\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.207px;\"\u003e\n\u003ctd valign=\"center\" style=\"width: 14.1276%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 83.468%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003e60 kDa\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.207px;\"\u003e\n\u003ctd valign=\"center\" style=\"width: 14.1276%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003eSpecific Activity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 83.468%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003e1,000,000 U\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.207px;\"\u003e\n\u003ctd valign=\"center\" style=\"width: 14.1276%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003eStorage buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 83.468%; height: 55.207px;\"\u003e\n\u003cp\u003e\u003cspan\u003e20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 74.8125px;\"\u003e\n\u003ctd valign=\"center\" style=\"width: 14.1276%; height: 74.8125px;\"\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 83.468%; height: 74.8125px;\"\u003e\n\u003cp\u003e\u003cspan\u003eOne unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze \u0026gt;95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20414ES92\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20414ES97\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20414-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e Endo H\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e10000 U\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e50000 U\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20414-B1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eBuffer 1 (10×)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e100 μL\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10000 U","offer_id":53294148125037,"sku":"20414ES92","price":70.0,"currency_code":"USD","in_stock":true},{"title":"50000 U","offer_id":53294159462765,"sku":"20414ES97","price":290.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20414.webp?v=1778483184"},{"product_id":"endo-s-bhz20800011","title":"Endo S","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eEndo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eProperties\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eEnglish synonym\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eEndo S\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e\u003ci\u003eE.coli\u003c\/i\u003e\u003ci\u003e \u003c\/i\u003erecombinant expression\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eDetected by SDS-PAGE, purity \u0026gt; 95%\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eStorage buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003ePBS pH 7.5\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eSpecific activity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e8,000 U\/mg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eThe definition of a unit is that under a total reaction volume of 10 μL, at 37 °C for 1 hour, 95% of the N-linked glycans in 5 μg of natural mouse IgG can be removed.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20413ES80\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20413ES90\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20413-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eEndo S\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e1000 U\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e5×1000 U\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e20413-B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e10×Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e5×1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe product can be stored at -15~-25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eReaction systerm\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eVolume\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eIgG\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eV\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eEndo S\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e10×Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/p\u003e\n\u0026lt;\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1000 U","offer_id":53294148190573,"sku":"20413ES80","price":70.0,"currency_code":"USD","in_stock":true},{"title":"5×1000 U","offer_id":53294158774637,"sku":"20413ES90","price":290.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20413ES.webp?v=1778483616"},{"product_id":"lyophilized-bst-plus-dna-polymerase-60-u-mu-l-glycerol-free-bhz20800085","title":"Lyophilized Bst Plus DNA Polymerase (60 U\/μL, Glycerol-Free)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003eHieff® Bst Plus DNA Polymerase is derived from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eBacillus stearothermophilus\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA Polymerase I. The 5 '-3' exonuclease activity was removed by genetic engineering method, but the 5 '-3' DNA polymerase activity and strong chain replacement activity were retained. Compared with wild-type Bst DNA Polymerase, the polymerase showed great improvement in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It was very suitable for isothermal amplification reactions such as LAMP reaction. This product freeze-dried glycerin-free Bst Plus DNA Polymerase to solve the problem of glycerinase-free instability and could be used to make freeze-dried products.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eHigh amplification efficiency\u003c\/li\u003e\n\u003cli\u003eHigh dUTP tolerance\u003c\/li\u003e\n\u003cli\u003eThe freeze-dried product form\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eIsothermal DNA amplification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003ctd\u003eBst DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeat Inactivation\u003c\/td\u003e\n\u003ctd\u003eIncubation at 85°C for 5 min.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eActivity Definition\u003c\/td\u003e\n\u003ctd\u003e1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e14405ES60 (12 KU)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e14405ES97 (120 KU)\u003c\/td\u003e\n\u003ctd\u003e14402ES98 (1,200 KU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14405-A\u003c\/td\u003e\n\u003ctd\u003eLyophilized Bst Plus DNA Polymerase (60 U\/μL，Glycerol-Free)\u003c\/td\u003e\n\u003ctd\u003e1 Vial（200 μL）\u003c\/td\u003e\n\u003ctd\u003e5 Vials(400 μL)\u003c\/td\u003e\n\u003ctd\u003e10 Vials(2 mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14405-B\u003c\/td\u003e\n\u003ctd\u003eLyophilized monoenzyme redissolution solution\u003c\/td\u003e\n\u003ctd\u003e200 μL\u003c\/td\u003e\n\u003ctd\u003e2 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e\u003cspan\u003eHigh Sensitivity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/384681\/131a-167524194263128440.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1: Sensitivity Test\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRed: 50 copies\/T, response less than 15 minutes\u003cbr\u003eFigure 1: The copy numbers of 50 copies\/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies\/T performed better, and the detection rate reached 100%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e\u003cspan\u003eStability\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/93756\/3580-167524288433759822.jpg\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 2.Stability testing at the same temperature\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRed: 37℃ 0 day  Blue: 37℃ 1 day  Green: 37℃ 3 days  Purple: 37℃ 5 days\u003cbr\u003e0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/384681\/95bb-167524291024348489.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 3. -20℃ repeated freeze-thaw stability test\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRed: Repeat freeze-thaw 0 times\u003cbr\u003eBlue: Repeat freeze-thaw 5 times\u003cbr\u003eGreen: Repeat freeze-thaw 10 times\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/713959\/894b-167524318728957537.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 4. 48-well NTC-\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"12 KU","offer_id":53294148354413,"sku":"14405ES60","price":225.0,"currency_code":"USD","in_stock":true},{"title":"120 KU","offer_id":53294159495533,"sku":"14405ES97","price":2105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/131a-167524194263128440_8aae251f-0c63-4fa9-bf6f-535768352375.png?v=1778101065"},{"product_id":"hieff-t7-rna-polymerase-gmp-grade-low-dsrna-250-u-mu-l-bhz20800067","title":"Hieff™ T7 RNA Polymerase (GMP-grade, low dsRNA, 250 U\/μL)","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003eThis is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003edsRNA level lower to about 1\/100000\u003c\/li\u003e\n\u003cli\u003eComptiable with Trilink CleanCap AG, LZCap\u003c\/li\u003e\n\u003cli\u003eHigh yields comparable to WT \u003c\/li\u003e\n\u003cli\u003eLower Cap input\u003c\/li\u003e\n\u003cli\u003eAnimal origin-free (AOF)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/blogs\/news\/low-dsrna-t7-rna-polymerase\"\u003e\u003cspan\u003ePlease find information on the development of this enzyme.\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eComponents\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629ES86\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(10 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(100 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(250 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(2,500 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"170\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10629\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff™ T7 RNA Polymerase (GMP Grrade, low dsRNA, 250 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"177\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e40 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"172\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e400 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"156\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"196\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eSpecifications \u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003eRecombinant\u003cem\u003e\u003ci\u003e \u003c\/i\u003e\u003c\/em\u003e\u003cem\u003e\u003ci\u003eE. coli \u003c\/i\u003e\u003c\/em\u003ewith T7 RNA Polymerase gene\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eOptimum Temperature\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003e37℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"301\"\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"982\"\u003e\n\u003cp\u003eThe amount of enzyme required to incorporate 1 nmol of [\u003csup\u003e3\u003c\/sup\u003eH] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended Mg2+\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e30mM Magnesium Acetate\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cstrong\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cstrong\u003e\u003cb\u003eQC \u003cspan\u003eStandard\u003c\/span\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eI\u003c\/span\u003etems\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eSpecification\/Standard\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnzyme\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eactivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e-\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e300U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProtein Purity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e≥95%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEndotoxin\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt;20 EU\/mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProtease\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExonuclease\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNickase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRNase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNegative\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eE.coli host protein\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt;50ppm\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eE\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e.coli host\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt;10 fg\/U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"425\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMycoplasma examination\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"731\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eRelated Products\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/blogs\/mrna\/gmp-grade-enzymes\"\u003e\u003cspan\u003eTris NTPs synergistically decreasing dsRNA\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10670\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e10× Transcription Buffer 2 GMP-grade 10670ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10657\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eN1-Me-Pseudo UTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan class=\"code\" style=\"font-size: 0.875rem;\"\u003e10657ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10656\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePseudo UTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan class=\"code\" style=\"font-size: 0.875rem;\"\u003e10656ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10652\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eATP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan class=\"code\" style=\"font-size: 0.875rem;\"\u003e10652ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10655\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eGTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan class=\"code\" style=\"font-size: 0.875rem;\"\u003e10655ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10653\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan class=\"code\" style=\"font-size: 0.875rem;\"\u003e10653ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/10654\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eUTP Tris Solution GMP-grade (100 mM) \u003c\/span\u003e\u003cspan class=\"code\" style=\"font-size: 0.875rem;\"\u003e10654ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeabio.com\/products\/36717\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eDouble-stranded RNA (dsRNA) ELISA kit 36717ES\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eFigures\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCustomer feedback:\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/dsRNA_figure_68789415-4b42-4894-84b5-a93b08e8d406.jpg?v=1714285432\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eFigure 1. Testing of T7 RNA Polymerase mutants. dsRNA Content: The dsRNA content reduced by more than 10-fold (A).  mRNA Integrity: Maintained at over 90% (B).  2K Fragment Capping Efficiency: Exceeded 99% (C, D).\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cdiv class=\"ListTitle\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"ListTitle\"\u003e\n\u003cspan class=\"code\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/Yields.jpg?v=1714284938\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/www.yeasenbiotech.com\/productdetail\/2858\"\u003e\u003cbr\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ListTitle\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eTable 1. The Yields of low dsRNA T7 RNA polymerase is comparable with that of WT.\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"ListTitle\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/t61_1024x1024.png?v=1717802537\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/h2\u003e\n\u003cp\u003eFigure 2. Evaluation of the immunogenicity of the IVT products in murine RAW264.7 cells (Figures 2A and 2B). IFN-β mRNA and protein levels were reduced in RAW264.7 cells transfected with mRNA produced by mutants compared to wild-type, indicating that mRNA synthesized by the wild-type T7 RNAP elicited the strongest immune response, while mRNA from the mutants showed a significantly reduced response.\u003c\/p\u003e\n\u003cp\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/T7_low_dsRNA_data_1024x1024.jpg?v=1718656756\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 3. The dsRNA content in mRNA synthesized with CleaScript™ T7 RNA polymerase is lower than that in mRNA synthesized with the wild-type enzyme after cellulose treatment.\u003c\/p\u003e\n\n\u003ch3\u003e\u003cstrong\u003e\u003cb\u003eShipping and Storage\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThe products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003ePublication:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"highwire-cite-title\" id=\"page-title\"\u003e\u003ca href=\"https:\/\/febs.onlinelibrary.wiley.com\/doi\/abs\/10.1111\/febs.70051\"\u003eEngineered T7 RNA polymerase reduces dsRNA formation by lowering terminal transferase and RNA-dependent RNA polymerase activities, The FEBS Journal 03 March, 2025 \u003c\/a\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"highwire-cite-title\"\u003e\u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.05.23.595468v2\"\u003eFADS and semi-rational design modified T7 RNA polymerase reduced dsRNA production, with lower terminal transferase and RDRP activities, bioxRxiv, 31 May, 2024\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\n\n\n\n\n\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 KU","offer_id":53294148387181,"sku":"10629ES10","price":198.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294156513645,"sku":"10629ES60","price":1675.0,"currency_code":"USD","in_stock":true},{"title":"250 KU","offer_id":53294156546413,"sku":"10629ES72","price":3775.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dsRNA_figure_68789415-4b42-4894-84b5-a93b08e8d406.jpg?v=1778101061"},{"product_id":"ucf-me-uracil-dna-glycosylase-udg-ung-heat-labile-1-u-mu-l-bhz20800089","title":"UCF.ME™ Uracil DNA Glycosylase(UDG\/UNG), heat-labile, 1 U\/μL","description":"\u003cp class=\"p\"\u003e\u003cspan\u003eUCF.ME Uracil DNA Glycosylase d\u003c\/span\u003e\u003cspan\u003eerived from psychrophilic marine bacteria, it is an ultra-low residual heat-labile UDG enzyme known as UCF.ME\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e. This product can prevent the residual activity of conventional UDG enzymes after inactivation from degrading dU-containing amplification products at room temperature. It also prevents background bacteria present in molecular enzymes from causing false positives in PCR results. Therefore, the introduction of UCF.ME\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eultra-low residual thermolabile UDG enzyme is crucial for accurately identifying infectious pathogens and assisting in the clinical diagnosis of infections and infectious diseases.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUltra-low residual of UCF.ME\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli \u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003egenomic DNA residue \u0026lt;0.1 copies\/ U;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLow nuclease residue\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eNo residual exonuclease, nicking enzyme, or RNase;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStrong digestion capability\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0.05 U\/T can digest 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecopies\/T dU-DNA products;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGood thermolability\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eComplete inactivation under any condition of 50\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003efor 10 minutes, 55\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003efor 5 minutes, or 95\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003efor 5 minutes;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCompatible with RT-qPCR reaction systems\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eNo impact on the detection system even at high input levels (2U\/20\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμ\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eL reaction system).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cdiv align=\"center\"\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"163\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca name=\"OLE_LINK3\"\u003e\u003c\/a\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca name=\"OLE_LINK2\"\u003e\u003c\/a\u003eRecombinant \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e with UDG gene from \u003c\/span\u003e\u003cspan\u003epsychrophilic marine bacteria\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"163\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emM Tris-HCl, 100 mM KCl, 0.1mM EDTA,1mM DTT, 0.5% Tween-20, 0.5% NP-40, 50% Glycerol, pH8.0 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e± \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0.2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e@\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"163\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOne unit (U) is defined as the amount of enzyme that \u003c\/span\u003e\u003cspan\u003erequired to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"25.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHeat Inactivation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e55℃\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003efor 5\u003c\/span\u003e\u003cspan\u003e min\u003c\/span\u003e\u003cspan\u003e; 50\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003efor 10\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emin\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"46.6600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"19.4200%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14466ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"18.6800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14466ES76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"15.2000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14466ES96\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10,000 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"46.6600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUCF.ME\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUracil DNA Glycosylase (UDG\/UNG), heat-labile, 1 U\/\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμ\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"19.4200%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμ\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"18.6800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμ\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.2000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe product should be stored at -25~\u003c\/span\u003e\u003cspan\u003e-15°C for 2 years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14466-1_600x600.jpg?v=1740122029\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure 1. 14466ES protein purity (SDS-PAGE) detection results. Protein Purity≥95%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_aa747c29-cfac-43c5-86b8-5d9ae84def0a.png?v=1738833578\"\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure 2. 14466ES Nuclease residue test results. No Residual Nucleases, No Residual Exonucleases, Nicking Enzymes, or RNases.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan class=\"15\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_1c0b3351-1cc9-4cc9-9f00-658d1728d836.png?v=1738833577\"\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure 3. 14466ES \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE.coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e genomic DNA residue test results. \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE.coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e genomic DNA residue\u0026lt; 0.1 copies\/ U\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003ethe residue levels were significantly lower than the conventional UDG.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_8f25b88a-bffb-4eb4-b001-c2e4e4ec6ab3.png?v=1738833578\"\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFigure 4.  Strong digestive capability. 0.05 U\/T is sufficient to digest 10^5 copies\/T of dU-DNA products. 14466ES was used to prepare qPCR mix, while the control group of qPCR mix without UDG enzyme could completely digest 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e copies\/T dU-DNA products. \u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp class=\"MsoNormal\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_a3c4d0b4-a89f-4888-94c9-f61ee76b8016.png?v=1738833578\"\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFigure 5.  After 50℃, 10min; 55℃, 5min; 55℃, 10min; 95℃, 5min heat inactivation treatment, 14466ES lost digestive ability.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_53a05239-361b-45e0-8fbd-ef9a320952ad.png?v=1738833578\"\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFigure 6. Compatible with the RT-qPCR reaction system, with no impact on the detection system even at high input levels (2U\/T).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294148419949,"sku":"14466ES60","price":150.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294155137389,"sku":"14466ES76","price":600.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294155170157,"sku":"14466ES96","price":8365.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/14466-1_600x600_8a149005-2dae-4130-875c-3bb325223a99.jpg?v=1778101064"},{"product_id":"t4-uvsx-recombinase-2-mu-g-mu-l-bhz20800075","title":"T4 UvsX Recombinase (2 μg\/μL)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp\u003eT4 UvsX Recombinase, derived from the T4 bacteriophage, is a homolog of the RecA\/Rad51 family and plays a critical role in the repair of double-strand DNA breaks and the restart of replication forks. This recombinase collaborates with other DNA-binding proteins or cofactors to form a nucleoprotein complex with single-stranded DNA (ssDNA). The complex identifies complementary regions on target DNA for hybridization, facilitating subsequent strand displacement reactions. The enzyme exhibits no nuclease activity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Components\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"112\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eNO.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"414\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"223\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e11079ES60 (100 μg)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"176\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e11079ES03\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e(1 mg)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e11079ES08\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e(5 mg)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"222\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e11079ES20\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e(20 mg)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"112\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003e11079\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"414\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eT4 UvsX protein (2 μg\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"223\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"176\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"233\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e2.5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"222\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrimarily used in isothermal amplification techniques, such as Recombinase Polymerase Amplification (RPA).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Storage Buffer Composition\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 20% Glycerol, pH 7.5 at 25°C.\u003c\/p\u003e\n\u003ch3\u003eShipping and Storage Conditions\u003c\/h3\u003e\n\u003cp\u003eShipped with ice packs; store at -20°C. Shelf life: 2 years. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003ch3\u003e Precautions\u003c\/h3\u003e\n\u003cp\u003e1. The glycerol content in the T4 UvsX Recombinase storage buffer is 20%. It is recommended to aliquot the enzyme into separate portions to avoid repeated freeze-thaw cycles.\u003cbr\u003e2. This product is intended for research use only.\u003cbr\u003e3. For your safety and health, please wear a lab coat and disposable gloves during handling.\u003c\/p\u003e\n                          \u003c\/div\u003e\u003c!--SECTION_BREAK--\u003e\u003cdiv class=\"payment-list\"\u003e\n                            \n                              \u003csvg class=\"payment-list__item\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" aria-labelledby=\"pi-american_express\" viewbox=\"0 0 38 24\" width=\"38\" height=\"24\"\u003e\u003ctitle id=\"pi-american_express\"\u003eAmerican Express\u003c\/title\u003e\n\u003cpath fill=\"#000\" d=\"M35 0H3C1.3 0 0 1.3 0 3v18c0 1.7 1.4 3 3 3h32c1.7 0 3-1.3 3-3V3c0-1.7-1.4-3-3-3Z\" opacity=\".07\"\u003e\u003c\/path\u003e\u003cpath fill=\"#006FCF\" d=\"M35 1c1.1 0 2 .9 2 2v18c0 1.1-.9 2-2 2H3c-1.1 0-2-.9-2-2V3c0-1.1.9-2 2-2h32Z\"\u003e\u003c\/path\u003e\u003cpath fill=\"#FFF\" d=\"M22.012 19.936v-8.421L37 11.528v2.326l-1.732 1.852L37 17.573v2.375h-2.766l-1.47-1.622-1.46 1.628-9.292-.02Z\"\u003e\u003c\/path\u003e\u003cpath fill=\"#006FCF\" d=\"M23.013 19.012v-6.57h5.572v1.513h-3.768v1.028h3.678v1.488h-3.678v1.01h3.768v1.531h-5.572Z\"\u003e\u003c\/path\u003e\u003cpath fill=\"#006FCF\" d=\"m28.557 19.012 3.083-3.289-3.083-3.282h2.386l1.884 2.083 1.89-2.082H37v.051l-3.017 3.23L37 18.92v.093h-2.307l-1.917-2.103-1.898 2.104h-2.321Z\"\u003e\u003c\/path\u003e\u003cpath fill=\"#FFF\" d=\"M22.71 4.04h3.614l1.269 2.881V4.04h4.46l.77 2.159.771-2.159H37v8.421H19l3.71-8.421Z\"\u003e\u003c\/path\u003e\u003cpath fill=\"#006FCF\" d=\"m23.395 4.955-2.916 6.566h2l.55-1.315h2.98l.55 1.315h2.05l-2.904-6.566h-2.31Zm.25 3.777.875-2.09.873 2.09h-1.748Z\"\u003e\u003c\/path\u003e\u003cpath fill=\"#006FCF\" d=\"M28.581 11.52V4.953l2.811.01L32.84 9l1.456-4.046H37v6.565l-1.74.016v-4.51l-1.644 4.494h-1.59L30.35 7.01v4.51h-1.768Z\"\u003e\u003c\/path\u003e\u003c\/svg\u003e\n\n                            \n                              \u003csvg class=\"payment-list__item\" version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" role=\"img\" x=\"0\" y=\"0\" width=\"38\" height=\"24\" viewbox=\"0 0 165.521 105.965\" xml:space=\"preserve\" aria-labelledby=\"pi-apple_pay\"\u003e\u003ctitle id=\"pi-apple_pay\"\u003eApple Pay\u003c\/title\u003e\n\u003cpath fill=\"#000\" d=\"M150.\u0026lt;p\u0026gt;\u0026lt;strong\u0026gt;Glycerol Content:\u0026lt;\/strong\u0026gt; Contains Glycerol\u0026lt;\/p\u0026gt;\"\u003e\u003c\/path\u003e\u003c\/svg\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1 mg","offer_id":53294148518253,"sku":"11079ES03","price":1010.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53294157103469,"sku":"11079ES60","price":145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/2022080117164387_f2d79c4e-7627-43c8-aa83-12e529d28143.webp?v=1778483098"},{"product_id":"hieff-unicon-hotstart-direct-dna-polymerase-50-u-mu-l-bhz20800069","title":"Hieff UNICON™ HotStart Direct DNA Polymerase, 50 U\/μL","description":"\u003cdiv class=\"Section0\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff Unicon Hotstart Direct Taq DNA Polymerase is a hotstart DNA polymerase\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ethat is resistant to blood and other inhibitors. The product is blocked by antibodies and has good amplification sensitivity and specificity. After heating  at the pre-denaturation temperature, the antibody is completely inactivated and the DNA polymerase activity is released. The amplification induced by nonspecific annealing of primers could be effectively inhibited by using the hotstart Taq enzyme.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"11.2000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eNo.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"7.2800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16.0600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10718ES00 \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e(500 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"17.1400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10718ES01\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e (5 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16.1800%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10718ES94 \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e(50 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16.1800%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10718ES95\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e (1250 KU)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"11.2000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10718\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"7.2800%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eHotstart\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eD-Taq (50 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16.0600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10 \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"17.1400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e100  μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16.1800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16.1800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe product should be stored at -20°C \u003c\/span\u003e\u003cspan\u003efor 2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eyears.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e1.  Reaction Composition\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"644\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"396\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eVolume (μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"273\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFinal Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"644\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2 \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e Buffera\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"396\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"273\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"644\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePrimer\/Probe Mixb\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"396\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eX\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"273\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.1 -0.5 \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eμM\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"644\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHotstart D-Taq (50 U\/μL)c\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"396\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.12\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"273\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.12 U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"644\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTemplate DNAb\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"396\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eX\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"273\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.1-100 ng\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"644\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eddH2O\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"396\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eto 50\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"273\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNote：Be sure to mix well before use, avoid excessive bubbles caused by\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eviolent \u003c\/span\u003e\u003cspan\u003evibration.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ea) According to the specific experimental application, it is needed to prepare the corresponding reaction\u003c\/span\u003e\u003cspan\u003e buffer. If a\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebasic buffer (10×) is required, Cat#11374 is recommended.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eb) The amount of DNA and the concentration of probes\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eor\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprimers\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eare\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003erecommen\u003c\/span\u003e\u003cspan\u003eded\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econcentrations. The\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eoptimal\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econcentration can be\u003c\/span\u003e\u003cspan\u003e adjusted according to the specific experimental conditions.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ec) The amount of enzyme can be adjusted according to the experimental applica\u003c\/span\u003e\u003cspan\u003etion.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e2.  Optimized Cycling Protocol\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"17\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"71\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"264\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eReaction stage\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"321\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eTemperature\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"358\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eTime\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"265\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eCycle\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"71\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"264\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eInitial denaturation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"321\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e95°C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"358\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emin\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"265\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" valign=\"top\" width=\"71\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\" valign=\"top\" width=\"264\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eAmplification\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ereaction\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"321\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e95°C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"358\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\" valign=\"top\" width=\"265\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e45 cycles\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"321\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e60°C\u003c\/span\u003e\u003cspan\u003ea\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"358\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esec\u003c\/span\u003e\u003cspan\u003eb\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNote：\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ea)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAmplification reaction: The temperature is adjusted according to the Tm\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003evalue of\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ethe designed primers.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eb)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eFluorescence\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esignal\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eacquisition:\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ePleas\u003c\/span\u003e\u003cspan\u003ee\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eset\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ethe\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eexperimental\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprocedure\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eaccording\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ethe\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003erequirements\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eof the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003einstrument manual.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294148682093,"sku":"10718ES00","price":150.0,"currency_code":"USD","in_stock":true},{"title":"5 KU","offer_id":53294156349805,"sku":"10718ES01","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20220425150010838.webp?v=1778483256"},{"product_id":"hieff-unicon-hotstart-e-taq-dna-polymerase-5-u-mu-l-bhz20800070","title":"Hieff UNICON™ HotStart E-Taq DNA Polymerase, 5 U\/μL","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eHieff UNICON™\u003c\/span\u003e\u003cspan\u003eHotstart E-Taq DNA Polymerase (Cat#10726ES) is a hot start DNA polymerase that employs the company's independently developed dual monoclonal antibodies for double closure. This product not only seals the 5'→3' polymerase activity of Taq DNA polymerase but also seals the 5'→3' exonuclease activity. Heating at the pre-denaturation temperature for 30 seconds can completely inactivate the closure antibodies, releasing the DNA polymerase activity and exonuclease activity. The dual closure feature not only effectively prevents non-specific amplification caused by mispairing or primer dimers but also effectively inhibits the decrease in fluorescence signals caused by probe degradation, providing dual protection to make in vitro diagnostic reagents more stable during transportation or use at room temperature. This Taq enzyme supports the development of a primer-probe pre-mixed system and offers higher sensitivity, specificity, and high GC sequence amplification capability compared to traditional qPCR reagents.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eHigh Specificity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eCapable of dual encapsulation, it simultaneously inhibits the 5'-3' Taq DNA polymerase activity and the 5'-3' exonuclease activity at room temperature, enhancing the specificity of the reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eNo Nuclease Contamination:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Free from contamination of exonucleases, endonucleases, and RNases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eSuitable for High-Sensitivity PCR Experiments:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Exhibits a good linear relationship when the template concentration ranges from 5×10^6 copies\/T to 5×10^1 copies\/T, with the lowest detection limit reaching 5 copies\/T.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eSuperior Stability:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e When formulated into a complete premix containing primers and probes, it remains stable after being left at 37°C for 14 days and after 50 freeze-thaw cycles.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eCompatible with Rapid Amplification Protocols\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e45 cycles\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003e results within 30 minutes.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e01 Good Sensitivity and Linear Range\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure shows that the qPCR master mix prepared with 10726 exhibits good linearity when the template concentration ranges from 5×10^6 copies\/T to 5×10^1 copies\/T\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e，\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eR2=0.9997\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_c720e33a-bb2a-4494-8a7b-56b1fbe4f528.png?v=1734339129\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e02 Excellent Thermal Accelerated Stability\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure 3 demonstrates the stability performance of qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template under various temperature and time conditions. The results indicate that the qPCR master mix formulated with 10726ES maintains stable performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10726_1_1_1024x1024.png?v=1748845948\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e03 Compatible with Rapid Cycling Programs\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e Figure shows that the qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template, can stably amplify and detect the limit concentration of template DNA\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e5 copies\/T\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e under both standard\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e80min\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003efast\u003cspan\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e30min\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e amplification programs.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_533f9ec2-1c87-4213-9f49-a5002044f8ff.png?v=1734339146\"\u003e\u003c\/span\u003e\u003cspan\u003eYeasen Biotech, with years of experience in the field of enzyme raw materials, has been providing high-quality and cost-effective molecular diagnostic PCR raw materials to IVD companies, third-party testing institutions, and research organizations. These include single components such as reverse transcriptase, RNase inhibitor, Taq DNA polymerase, uracil DNA glycosylase (UDG\/UNG enzyme), Taq enzyme antibody, dNTPs, as well as system-optimized qPCR\/RT-qPCR premix solutions, offering superior performance and stable enzyme raw materials for the development of clinical\/non-clinical diagnostic kits.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eProduct recommendation\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eProduct type\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eProduct Name\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eCat No.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003ePCR enzyme series\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/3865\"\u003eHieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U\/μL\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10726ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1668\"\u003eHifair™\u003cspan style=\"font-family: Times New Roman;\"\u003e V Reverse Transcriptase(200 U\/μL)\u003c\/span\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e11300ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1227\"\u003eUracil DNA Glycosylase(UDG\/UNG),heat-labile,1 U\/μL\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10303ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/536\"\u003eMurine RNase Inhibitor(40 U\/µL)\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10603ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/267\"\u003edNTP Mix(25 mM each)\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10125ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\"\u003eUltra-clean PCR single enzyme series with ultra-low host residue\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5776\"\u003eHieff UCF.ME™ Hotstart Sensitive Taq DNA Polymerase (5 U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14314ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5001\"\u003eHifair UCF.ME™ V Reverse Transcriptase (200 U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14608ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5780\"\u003eUCF.ME™ Uracil DNA Glycosylase(UDG\/UNG), heat-labile, 1 U\/μL\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14466ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/4874\"\u003eUCF.ME™ Murine RNase Inhibitor(40 U\/µL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14672ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\"\u003eLyo-Ready PCR monoenzyme series\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5443\"\u003eHieff UNICON™ HotStart E-Taq DNA Polymerase, Glycerol-free (5U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14316ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1710\"\u003eHifair™ V Reverse Transcriptase, Glycerol-free(600 U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e11301ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1870\"\u003eUracil DNA Glycosylase(UDG),Heat-Labile(1 U\/μL,Glycerol-Free)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e10707ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1869\"\u003eMurine RNase Inhibitor(200 U\/µL,Glycerol-free)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e10703ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"250 U","offer_id":53294148747629,"sku":"10726ES72","price":55.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294157463917,"sku":"10726ES80","price":185.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294157496685,"sku":"10726ES92","price":1415.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_c720e33a-bb2a-4494-8a7b-56b1fbe4f528.png?v=1778101061"},{"product_id":"hifair-super-reverse-transcriptase-glycerol-free-600-u-mu-l-bhz20800103","title":"Hifair™ Super Reverse Transcriptase, Glycerol-free (600 U\/μL)","description":"\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n\u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"card__section\"\u003e\n\u003cdiv class=\"rte text--pull\"\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eHifair™\u003c\/span\u003e\u003cspan\u003e Super Reverse Transcriptase\u003c\/span\u003e\u003cspan\u003e is an updated version of Hieff™M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 70°C) than Hieff™\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eM-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eV Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eFeatures \u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSuitable for RT-LAMP applications.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGlycerol-free, Suitable for lyophilization.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eOne unit (U) of enzyme activity is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material within 10 minutes at 37°C, using Poly(A).Oligo(dT) as the template-primer.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eComponent \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003ctable cellspacing=\"4\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponent \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e14618ES12\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e14618ES75\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14618\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHifair™  Super Reverse Transcriptase, Glycerol-free (600 U\/μL) \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage Conditions\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eStore at 4˚C for up to 6 months. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cspan\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"12 KU","offer_id":53294148845933,"sku":"14618ES12","price":315.0,"currency_code":"USD","in_stock":true},{"title":"120 KU","offer_id":53294156218733,"sku":"14618ES75","price":2375.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/90267_cf391acb-ad76-4e0f-b634-cb6d0ee95d3c.webp?v=1778112442"},{"product_id":"recombinant-enterokinase-high-concentration-expressed-in-e-coli-bhz20800029","title":"Recombinant Enterokinase, High Concentration, Expressed in E.coli.","description":"\u003cp\u003eRecombinant Enterokinase (rEK) is a highly pure fragment of the recombinant bovine enterokinase light chain. Its amino acid sequence is identical to that of the bovine enterokinase light chain, featuring enzymatic cleavage sites that are as specific as those found in naturally extracted enterokinase, with cutting sites at Asp-Asp-Asp-Asp-Lys. This enables the removal of the fusion protein located at the N-terminus of the protein, eliminating unnecessary fusion tags. Additionally, rEK exhibits higher cleavage activity compared to the natural enzyme.\u003c\/p\u003e\n\u003cp\u003eThe Recombinant Enterokinase is a high-purity, highly active, and highly specific bovine enterokinase produced through recombinant expression in Escherichia coli. Devoid of other proteases, it can effectively cleave fusion proteins over a broad pH range (4.5-9.5) and a wide temperature range. It retains partial activity even in the presence of various detergents and denaturing agents. This product is label-free, and due to its extremely high enzymatic cleavage activity, minimal amounts are needed for cleavage reactions, ensuring no interference with downstream protein applications, making removal unnecessary.\u003c\/p\u003e\n\u003ch3\u003eFeature\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity:\u003c\/strong\u003e The enzyme exhibits specificity for a particular amino acid sequence, cleaving at the carboxyl-terminal site containing four aspartic acids followed by lysine (DDDDK).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnimal free:\u003c\/strong\u003e Produced through recombinant methods, ensuring no contamination from exogenous viruses. The production process does not involve any animal-derived materials.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQuality stability:\u003c\/strong\u003e Batch production guarantees consistently stable batches, with no variations in quality between different batches.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity:\u003c\/strong\u003e Devoid of other proteases, ensuring non-specific cleavage.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompliance with regulations:\u003c\/strong\u003e Production facilities and environments adhere to relevant regulatory requirements. The manufacturing process adheres to GMP guidelines.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComprehensive quality documentation:\u003c\/strong\u003e Can provide relevant regulatory support documents as per customer requirements.\u003c\/p\u003e\n\u003ch3\u003eSpecification\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eExpressed in \u003cem\u003eE.coli.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55px;\"\u003e\n\u003ctd style=\"height: 55px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e25.85 kDa\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eAppearance\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003eClear, colorless liquid.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/b\u003e\u003cb\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e≥5 U\/uL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 94px;\"\u003e\n\u003ctd style=\"height: 94px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eActivity Definition\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 94px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003eOne unit of activity is defined as the amount of enzyme required to cleave 95% of\u003cstrong\u003e 500 μg\u003c\/strong\u003e of a 30KD fusion protein containing enterokinase cleavage sites, under the following conditions: 25°C, 12-16 hours, in a buffer system consisting of 25 mM Tris-HCl, pH 8.0.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e＜1 EU\/μg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eHCP\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e≤0.01%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30.7396px;\"\u003e\n\u003ctd style=\"height: 30.7396px; width: 35.9356%;\"\u003e\u003cb\u003eTag\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 30.7396px; width: 61.2969%;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55px;\"\u003e\n\u003ctd style=\"height: 55px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e50 mM Tris-HCl，pH8.0，250 mM NaCl，2 mM CaCl2，50% Glycerol\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e\u003cspan\u003eThe amount of recombinant protein cleaved per unit of Enterokinase (EK) \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center;\"\u003e\n\u003cp\u003eSuppliers\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.0171%; text-align: center;\"\u003e\n\u003cp\u003e Yeasen 5 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 34.9829%; text-align: center;\"\u003e\n\u003cp\u003e Thermo 1 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26%; text-align: center;\"\u003e\n\u003cp\u003e NEB 16 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center;\"\u003e\n\u003cp\u003eActivity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.0171%; text-align: center;\"\u003e\n\u003cp\u003e500 μg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 34.9829%; text-align: center;\"\u003e\n\u003cp\u003e20 μg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26%; text-align: center;\"\u003e\n\u003cp\u003e25 μg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cbr\u003e\u003c\/h3\u003e\n\u003ch3\u003eStorage \u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eStore at -25 to -15°C for 1 years. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294148911469,"sku":"20401ES60","price":60.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294155202925,"sku":"20401ES76","price":255.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20401.webp?v=1778113412"},{"product_id":"recombinant-cpb-expressed-in-e-coli-gmp-grade-bhz20800027","title":"Recombinant CPB, Expressed in E.coli. GMP-grade","description":"\u003cp class=\"p\"\u003e\u003cspan\u003eCarboxypeptidase B, also known as peptidyl-L-lysine (L-lysine) hydrolase or pancreatic carboxypeptidase B, is a specialized enzyme that hydrolyzes the amino group at the C-terminus of basic amino acids in proteins (Lysine (Lys, K), Arginine (Arg, R), and Histidine (His, H)). It has a molecular weight of 33.8 kD and an isoelectric point of 6.0, with an optimal pH range of 7.5 to 9.0. The activity of carboxypeptidase B is subject to competitive inhibition by arginine and lysine, and is inhibited by metal ion chelators such as EDTA.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eYeasen UCF.ME™\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eR\u003c\/span\u003e\u003cspan\u003eecombinant \u003c\/span\u003e\u003cspan\u003eCP\u003c\/span\u003e\u003cspan\u003eB is expressed in E.coli, produced under GMP regulations, free of any animal-derived components, and without the risk of viral contamination from animal sources. The amino acid sequence is identical to that of rat pancreatic carboxypeptidase B, possessing the same enzymatic properties as the animal-derived enzyme, and can be used as a substitute in various biotechnological processes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003eStrong specificity\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eA\u003c\/span\u003e\u003cspan\u003e specialized enzyme that hydrolyzes the amino group at the C-terminus of basic amino acids in proteins (Lysine (Lys, K), Arginine (Arg, R), and Histidine (His, H))\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eH\u003c\/span\u003e\u003cspan\u003eigh purity—\u003c\/span\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e≥95%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eAnimal free\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eRecombinantly produced, free from exogenous viral contamination, and no animal-derived materials are used in the production process.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eProduction of recombinant insulin and its analogs.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eDetermination of the C-terminal amino acids of proteins\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eRemoval of the C-terminal histidine tag from proteins\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eProduction of other recombinant polypeptide substances\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eEnzymatic synthesis of certain special compounds\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.1000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eE.coli \u003c\/span\u003e\u003cspan\u003erecombinant expression\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.1000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTheoretical value 33.8±3.4 kDa\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAppearance\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.1000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eWhite, off-white, or pale yellow powder\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8800%\"\u003e\n\u003cp\u003eEnzyme Concentration\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.1000%\"\u003e\n\u003cp\u003e≥170 USP units\/mg pro\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit\u003c\/span\u003e\u003cspan\u003e Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.1000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAt 25℃, pH 7.6, the amount of enzyme that catalyzes 1 μmoL of hippuryl-L-arginine hydrolysis in 1 minute is defined as one unit of enzyme activity\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eQuality Assurance\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"74.1000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSDS-PAGE gel detection shows a clear single band of the target protein; no other proteases present, no non-specific cutting.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\" style=\"width: 99.9137%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.7379%;\" valign=\"center\" width=\"17.3600%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 36.1987%;\" valign=\"center\" width=\"40.0600%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 20.5551%;\" valign=\"center\" width=\"21.8400%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e2041\u003c\/span\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e03\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 20.5551%;\" valign=\"center\" width=\"20.7200%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20417ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22.7379%;\" valign=\"center\" width=\"17.3600%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e41\u003c\/span\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 36.1987%;\" valign=\"center\" width=\"40.0600%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eU\u003c\/span\u003eCF.ME™ Recombinant CPB（Powder）\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 20.5551%;\" valign=\"center\" width=\"21.8400%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e1 \u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 20.5551%;\" valign=\"center\" width=\"20.7200%\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e10 mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLyophilized powder\u003c\/span\u003e\u003cspan\u003e can be stored a\u003c\/span\u003et 2 ~ 8℃ for two y\u003cspan\u003eear\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1 mg","offer_id":53294148878701,"sku":"20417ES03","price":105.0,"currency_code":"USD","in_stock":true},{"title":"10 mg","offer_id":53294157889901,"sku":"20417ES10","price":585.0,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53294157922669,"sku":"20417ES80","price":2895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/universe_d016a982-0200-40bc-98ba-675af9decf23.webp?v=1778112772"},{"product_id":"t7-rna-polymerase-gmp-grade-250-u-mu-l-bhz20800044","title":"T7 RNA Polymerase GMP-grade (250 U\/μL)","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003eThis product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003cp\u003eThis product is produced in accordance with GMP regulations and provided in liquid form.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSynthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template\u003c\/li\u003e\n\u003cli\u003eHigher yield and easy to operate\u003c\/li\u003e\n\u003cli\u003eLower endotoxins\u003c\/li\u003e\n\u003cli\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRadiolabeled RNA probe preparation\u003c\/li\u003e\n\u003cli\u003eRNA generation for studies of RNA structure, processing and catalysis\u003c\/li\u003e\n\u003cli\u003eExpression control via anti-sense RNA\u003c\/li\u003e\n\u003cli\u003emRNA, sgRNA synthesis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eExpressed in\u003cem\u003e E. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ewith a recombinant T7 RNA Polymerase gene \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\n\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\n\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003eQuality\u003c\/h2\u003e\n\u003ctable width=\"1089\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eCOA Items\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eStandard\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eActivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e250-300U\/μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\n\u003cspan\u003eProtein\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econcentration\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e≥0.5mg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e≥95%\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eEndotoxin\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e\u0026lt;20 EU\/mg\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual Protease\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual Exonuclease\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual Nickase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual RNase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\n\u003cspan\u003eE.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecoli host protein\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e\u0026lt;50ppm\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\n\u003cspan\u003eE.coli host\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e\u0026lt;10 fg\/U\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eMycoplasma DNA\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10625ES10 (10 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES60 (100 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES86 (2,500 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES99 (100 MU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10625\u003c\/td\u003e\n\u003ctd\u003eT7 RNA Polymerase GMP-grade (250 U\/μL)\u003c\/td\u003e\n\u003ctd\u003e40 μL\u003c\/td\u003e\n\u003ctd\u003e400 μL\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e400 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eT7 RNA Polymerase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/693866\/322e-167030612134953504.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/seas.ysbuy.com\/file\/98179\/8309-167030624349998928.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigures 2. The transcription demonstration of different lengths RNA by T7 kit respectively in the electrophoretogram (Figures 2A), the capillary electrophoresis diagram (Figures 2B), and the chromatogram (Figures 2C)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0803\/9419\/1166\/files\/T7_480x480.png?v=1700873194\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 3. Synthesis of capped RNA in vitro.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 3A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 3B.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 KU","offer_id":53294148977005,"sku":"10625ES10","price":140.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294159200621,"sku":"10625ES60","price":1025.0,"currency_code":"USD","in_stock":true},{"title":"250 KU","offer_id":53294159233389,"sku":"10625ES72","price":2401.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/322e-167030612134953504.png?v=1778101062"},{"product_id":"hieff-unicon-hotstart-e-taq-dna-polymerase-20-u-mu-l-bhz20800078","title":"Hieff UNICON™ HotStart E-Taq DNA Polymerase, 20 U\/μL","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eHieff UNICON™ Hotstart E-Taq DNA Polymerase features an enhanced hot start Taq DNA polymerase, incorporating dual blocking antibodies. Both the 5'→3' polymerase and 5'→3' exonuclease activities of Taq DNA polymerase are effectively inhibited. A 30-second heating step at the pre-denaturation temperature serves to completely deactivate the antibodies, restoring DNA polymerase and exonuclease activities. This dual blocking capability not only safeguards against nonspecific amplification due to mismatch or primer dimer but also proficiently restrains the reduction in fluorescence signal caused by probe degradation. This ensures enhanced stability of the in vitro detection reagent during transportation or room temperature usage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh sensitivity\u003c\/li\u003e\n\u003cli\u003eeffectively inhibit the non-specific amplification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine PCR amplification of DNA fragments\u003c\/li\u003e\n\u003cli\u003eHotstart PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003ctd\u003eTaq DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurity\u003c\/td\u003e\n\u003ctd\u003e≥ 95% (SDS-PAGE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHot Start\u003c\/td\u003e\n\u003ctd\u003eBuilt-In Hot Start\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction Speed\u003c\/td\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExonuclease Activity\u003c\/td\u003e\n\u003ctd\u003e5' - 3'\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable border=\"1\" cellpadding=\"1\" cellspacing=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e13824ES72\u003cbr\u003e(250 U)\u003c\/td\u003e\n\u003ctd\u003e13824ES76\u003cbr\u003e(500 U)\u003c\/td\u003e\n\u003ctd\u003e13824ES80\u003cbr\u003e(1000 U)\u003c\/td\u003e\n\u003ctd\u003e13824ES92\u003cbr\u003e(10000 U)\u003c\/td\u003e\n\u003ctd\u003e13824ES93\u003cbr\u003e(25000 U)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e13824\u003c\/td\u003e\n\u003ctd\u003eHotstart E-Taq DNA Polymerase, 20 U\/μL\u003c\/td\u003e\n\u003ctd\u003e12.5 μL\u003c\/td\u003e\n\u003ctd\u003e25 μL\u003c\/td\u003e\n\u003ctd\u003e50 μL\u003c\/td\u003e\n\u003ctd\u003e500 μL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThe product is shipped with dry ice and can be stored at -15°C~-25°C for 2 years.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1000 U","offer_id":53294149108077,"sku":"13824ES80","price":185.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294158479725,"sku":"13824ES92","price":1415.0,"currency_code":"USD","in_stock":true},{"title":"25000 U","offer_id":53294158512493,"sku":"13824ES93","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10726.webp?v=1778483131"},{"product_id":"hieff-unicon-hotstart-e-taq-dna-polymerase-glycerol-free-5-u-mu-l-bhz20800081","title":"Hieff UNICON™ HotStart E-Taq DNA Polymerase, Glycerol-free (5 U\/μL)","description":"\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eHieff UNICON\u003csup\u003eTM\u003c\/sup\u003e HotStart E-Taq DNA Polymerase, Glycerol-free is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature. This product contains no Glycerol and is specially designed for the preparation of lyophilized reagents.\u003c\/p\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES76 \/ 14316ES80 \/ 14316ES92 \/\u003cspan\u003e \u003c\/span\u003e14316ES97 \/\u003cspan\u003e \u003c\/span\u003e14316ES98\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e500 U \/ 1000 U \/ 10 KU \/\u003cspan\u003e \u003c\/span\u003e50 KU \/\u003cspan\u003e \u003c\/span\u003e100 KU\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 540px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 181px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES76\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 84.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 72.3828px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES92\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 181px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eHieff UNICON\u003csup\u003eTM\u003c\/sup\u003e HotStart E-Taq DNA Polymerase, Glycerol-free (5 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e100 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 84.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 72.3828px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e2×1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eFigure\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14316.jpg?v=1719509322\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eThis product should be stored at 2~8℃ for 1 years.\u003c\/p\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1.Reaction Setup\u003c\/p\u003e\n\u003ctable style=\"width: 544px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eComponents\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eVolume (μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eFinal Concentration\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e2×Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e25\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1×\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003ePrimer\/Probe mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eX\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e0.1\u003cspan\u003e \u003c\/span\u003eμmol\/L-0.5\u003cspan\u003e \u003c\/span\u003eμmol\/L\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eHieff UNICON\u003csup\u003eTM\u003c\/sup\u003e HotStart E-Taq DNA Polymerase, Glycerol-free (5 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1.2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e0.12 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eDNA template\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eX\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e0.1-100 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eup to 50\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cstrong\u003e-\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e*According to the specific experimental application, the corresponding reaction Buffer should be prepared by oneself. The amount of DNA and primer concentration in the above table are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.\u003c\/p\u003e\n\u003col start=\"2\"\u003e\n\u003cli data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eThermal cycling protocol (2-Step cycling protocol)\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eStage\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eTemperature\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eTime\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eCycles\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003ePre-denaturation\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e95℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e5 min\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eDenaturation\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e95℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e15 sec\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e45\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eAnnealing\/Extension\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e60℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e30 sec\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e*The reaction temperature is adjusted according to the Tm value of the designed primers. Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.\u003c\/p\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1.This product is for scientific research purposes only.\u003c\/p\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e2.Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.\u003c\/p\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan data-mce-style=\"font-weight: 550;\" style=\"font-weight: 550;\"\u003eDocuments:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003ca class=\"down\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" target=\"_blank\" title=\"download\"\u003e\u003cimg alt=\"\" src=\"data:image\/png;base64,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\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"top_tabc\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"cwrap\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\u003ca class=\"down\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" target=\"_blank\" title=\"download\"\u003e\n\u003cp\u003e14316_Manual_Ver.EN20230605.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan data-mce-style=\"font-weight: 550;\" style=\"font-weight: 550;\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294149239149,"sku":"14316ES76","price":105.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294158184813,"sku":"14316ES80","price":185.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":53294158217581,"sku":"14316ES92","price":1415.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10726_ed5a566f-ba3b-4d40-be3a-76a5f73f10ca.webp?v=1778483908"},{"product_id":"ucf-me-ultranuclease-gmp-grade-250-u-mu-l-bhz20800050","title":"UCF.ME™ UltraNuclease GMP-grade (250 U\/μL)","description":"\u003cp style=\"font-weight: 400;\"\u003eUltraNuclease is a genetically engineered endonuclease from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eSerratia marcescens. \u003c\/em\u003eThe enzyme digests all forms of DNA\/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein\/polysaccharide pharmaceutical manufacturing.  It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eUltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eWide range of applications: degrade all forms of DNA and RNA \u003c\/li\u003e\n\u003cli\u003eHigh purity and activity: purity ≥ 99%; specific activity ≥ 1.5×10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eU\/mg\u003c\/li\u003e\n\u003cli\u003eStrong adaptability: strong tolerance, adapt to a variety of reaction conditions\u003c\/li\u003e\n\u003cli\u003eComply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin\u003c\/li\u003e\n\u003cli\u003eQualification: product gets the FDA DMF filling which can shorten the customers for new drug application\u003c\/li\u003e\n\u003cli\u003eGMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003ePurification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA\/RNA from proteins and other biologicals\u003c\/li\u003e\n\u003cli\u003eReduction of viscosity caused by nucleic acids\u003c\/li\u003e\n\u003cli\u003eSample preparation in electrophoresis and chromatography\u003c\/li\u003e\n\u003cli\u003ePrevention of cell clumping\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable height=\"257\" style=\"width: 812px; height: 245px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003eRecombinant E. coli with UltraNuclease gene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003e26.5 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003eIsoelectric point\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003e6.85\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003e≥ 99% (SDS-PAGE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 27.9818px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 27.9818px;\"\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 27.9818px;\"\u003e20 mM Tris-HCl pH8.0, 2 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 20 mM NaCl, 50% glycerin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 95.7812px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 95.7812px;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 95.7812px;\"\u003eThe definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.027%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.2804%;\"\u003eComponents No.\u003c\/td\u003e\n\u003ctd style=\"width: 18.4883%;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20157ES25 (25 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 15.2176%;\"\u003e20157ES60 (100 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20157ES80 (1 MU)\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20157ES90 (5 MU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.2804%;\"\u003e20157\u003c\/td\u003e\n\u003ctd style=\"width: 18.4883%;\"\u003eUltraNuclease GMP-grade (250 U\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.2176%;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e4 mL\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eQuality\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNo.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eTest Items\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eCompetitor\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e Yeasen\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eE. coli\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eE. coli\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eActivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥ 250 units\/μL \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e250-300 units\/μL \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e3\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSpecific Activity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥1.1×10\u003csup\u003e6\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003eU\/mg\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥1.5×10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eU\/mg\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e4\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥ 99%（SDS）\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥ 99%（HPLC）\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e\u0026lt; 0.25EU\/1000U\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e\u0026lt; 0.25EU\/1000U\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e6\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eProtease\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNone Detected\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNone Detected\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cul\u003e\n\u003cli align=\"justify\" class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eUltraNuclease \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eEnzymatic \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eActivity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003cspan style=\"font-family: Inter Medium;\"\u003eUnder Different Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eTemperature \u0026amp; pH\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 1. Effects of temperature and pH on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_f3e27588-04cc-429b-af21-66c8e25bfd62_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 1. Effects of temperature and pH on enzyme activity.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e(A) Enzyme activity increases with temperature, with the optimum at 37 °C.\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e(B) The enzyme is active within pH 4–10, with pH 8.0 as the optimum.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eSalt ion concentration\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 2. Effects of salt ion concentration on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_b380caa3-f787-416c-a1fe-c67e9fdb42df_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eFigure \u003c\/span\u003e2\u003cspan class=\"15\"\u003e. Effects of salt ion concentration on enzyme activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(A). Na⁺\/K⁺ inhibit enzyme activity; activity is completely lost above 300 mM, while good activity is retained below 20 mM.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(B). Maximum activity is observed with 5 mM Mg²⁺; in the absence of Mg²⁺, 1–2 mM Mn²⁺ can support activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(C). Increasing PO₄³⁻ concentration significantly inhibits enzyme activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eSurfactants\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 3. Effects of surfactants on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_9203e49b-bc65-4eaf-b571-73449d80442d_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 3. Effects of surfactants on enzyme activity.\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e(A) Triton® X-100 and (B) sodium deoxycholate reduce enzyme activity and should be maintained at low concentrations.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eProtein denaturants\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 4. Effect of protein denaturants on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_05d55a6a-9393-4b2e-b737-f31479947c4c_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 4. Effect of protein denaturants on enzyme activity.\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003eSDS and urea reduce enzyme activity and should be maintained at low concentrations.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eOther commonly used reagents\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 5. Effects of different inhibitors on UltraNuclease activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_c0ddd62f-a3fa-4e0f-b1db-42ada7393697_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eFigure\u003c\/span\u003e 5\u003cspan class=\"15\"\u003e. Effects of different inhibitors on UltraNuclease activity.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eA\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. Enzyme activity is strongly influenced by ammonium sulfate concentration.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eB\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. PMSF shows little effect on enzyme activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eC\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. At low EDTA concentrations (≤0.5 mM), enzyme activity is largely unaffected, whereas at 5 mM EDTA, activity is completely inhibited.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_7ffd81d4-9f52-4bad-afc1-93f728c332b7_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cul\u003e\n\u003cli align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eRobust Performance\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 6. Single-factor test comparison with Supplier M*.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_899fdedb-9633-4060-99d0-10ff8921c18d_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 6. Single-factor test comparison with Supplier M*.\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003eNo significant difference was observed between Yeasen UltraNuclease and supplier M*.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003eStrong stability\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 7. Stability of UltraNuclease.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/8_72f7094f-ba1f-4f6f-b950-f4ff92ff503f_1024x1024.png?v=1757913475\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003eFigure 7. Stability of UltraNuclease.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(A) Enzyme activity remained unchanged after 4 weeks of storage at –20 °C and 4 °C, and after 2 weeks at 37 °C.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(B) Enzyme activity was unaffected after 10 freeze–thaw cycles between –80 °C and –25 °C.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eReaction conditions recommend\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eTable 1. The optimal reaction conditions for UCF.ME™ UltraNuclease\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFactor\u003c\/td\u003e\n\u003ctd\u003eOptimum Condition\u003c\/td\u003e\n\u003ctd\u003eEffective condition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMg\u003csup\u003e2+\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e1-5mM\u003c\/td\u003e\n\u003ctd\u003e0-10mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH\u003c\/td\u003e\n\u003ctd\u003e8-9\u003c\/td\u003e\n\u003ctd\u003e6-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTemperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003ctd\u003e0-42℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNa+\/K+\u003c\/td\u003e\n\u003ctd\u003e0-20mM\u003c\/td\u003e\n\u003ctd\u003e0-150mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePO\u003csub\u003e4\u003c\/sub\u003e\u003csup\u003e3+\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e0-10mM\u003c\/td\u003e\n\u003ctd\u003e0-100mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA\u003c\/td\u003e\n\u003ctd\u003e0-0.5mM\u003c\/td\u003e\n\u003ctd\u003e0-5mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTriton®X-100\u003c\/td\u003e\n\u003ctd\u003e0%-0.4%\u003c\/td\u003e\n\u003ctd\u003e0%-1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"25 KU","offer_id":53294149337453,"sku":"20157ES25","price":155.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294159266157,"sku":"20157ES60","price":355.0,"currency_code":"USD","in_stock":true},{"title":"1 MU (1000 KU)","offer_id":53294159298925,"sku":"20157ES80","price":1590.0,"currency_code":"USD","in_stock":true},{"title":"5 MU (5000 KU)","offer_id":53294159331693,"sku":"20157ES90","price":3085.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1024x1024_194d99bf-687a-4a20-993d-99e720b68522.png?v=1778101064"},{"product_id":"hieff-unicon-hotstart-taq-dna-polymerase-5-u-mu-l-bhz20800072","title":"Hieff UNICON™ HotStart Taq DNA Polymerase, 5 U\/μL","description":"\u003cp align=\"justify\" class=\"18\"\u003e\u003cspan\u003eHieff UNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e HotStart Taq DNA Polymerase is a mixture of Hieff UNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq Antibody\u003cspan\u003e(\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eCatalog #31301ES\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eand Hieff\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan\u003e Taq DNA Polymerase (Catalog #10101ES). Hieff UNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq Antibody had a high affinity for Hieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq DNA Polymerase, and the activity of Hieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq DNA Polymerase could still be blocked after being treated at 50℃ for 30 min. The product was completely inactivated by heating at pre denaturation temperature for 30 seconds, releasing DNA polymerase activity. The hot start Taq enzyme can effectively inhibit the amplification caused by non-specific annealing of primers.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"18\"\u003e\u003cspan\u003eHieff UNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e HotStart Taq DNA Polymerase is suitable for hot start PCR and qPCR. Hieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq DNA Polymerase in this product is a heat stable recombinant DNA polymerase with high template affinity, which is very suitable for amplification of low copy template. The amplified product has 3 '- dA and can be easily cloned into T vector.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eF\u003c\/span\u003e\u003cspan class=\"15\"\u003ee\u003c\/span\u003e\u003cspan class=\"15\"\u003eatures\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoBodyText\"\u003e\n\u003c!--[endif]--\u003e\u003cspan\u003eClosed Detection of DNA Polymerase Activity\u003cspan\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eIn the Hieff UNICON\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e HotStart Taq Buffer (Mg2+  Plus) \u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003ethe activity release was less than 5% after incubation at 65℃ for 30 min.\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoBodyText\"\u003eDNA Polymerase Activity Release Detection \u003cspan\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eIn the HieffUNICON\u003c\/span\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e HotStart Taq Buffer (Mg2+  Plus) \u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003ethe activity release was higher than 95% when heated at 95℃ for 30 sec.\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoBodyText\"\u003eExonuclease Activity: In 20 μL reaction system, 10 U of this product and 0.6 μg of λ - HindIII were incubated at 74℃ for 1 h, and the electrophoresis band of DNA had no change.\u003c\/li\u003e\n\u003cli class=\"MsoBodyText\"\u003eEndonuclease Activity: In 20 μL reaction system, 10 U of the product and 0.6 μg of supercoiled pBR322 DNA were incubated at 74 ℃ for 1 h, and the DNA electrophoretic bands did not change.\u003c\/li\u003e\n\u003cli class=\"MsoBodyText\"\u003eResidual \u003ci style=\"font-size: 0.875rem;\"\u003eE.coli\u003c\/i\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e gDNA Test: The E.coil 16S rDNA gene was amplified by adding 2 U of the product into 50 μL system and using sterile ddH2O as template. After 35 cycles, the amplified products were subjected to 1% agarose gel electrophoresis and EB staining without amplification bands.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eSpecifications \u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePolymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTaq DNA Polymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e≥ 95%\u003c\/span\u003e\u003cspan\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eSDS-PAGE\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBuilt-In Hot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eReaction Speed\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStandard\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExonuclease Activity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5' - 3'\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUsing the activated DNA of salmon sperm as template \/ primer, the activity was defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"189\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"109\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10729ES72 (250 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"108\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10729ES76\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e(500 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"116\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10729ES80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(1000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"115\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10729ES92 (10000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"116\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10729ES93 (25000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"189\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHotStart Taq DNA Polymerase (5 U\/μL)  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"109\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"108\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"116\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"115\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e  1 mL×2   \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"116\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL×5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for \u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"250 U","offer_id":53294149435757,"sku":"10729ES72","price":50.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294155694445,"sku":"10729ES76","price":100.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294155727213,"sku":"10729ES80","price":205.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294155759981,"sku":"10729ES92","price":1870.0,"currency_code":"USD","in_stock":true},{"title":"25000 U","offer_id":53294155792749,"sku":"10729ES93","price":4760.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10726_3fae8cb0-95a2-4ff9-8a9a-d2799268cbc3.webp?v=1778112116"},{"product_id":"ides-protease-40-u-mu-l-bhz20800121","title":"IdeS Protease (40 U\/μL)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eIdeS Protease, full name Immunoglobulin G-degrading enzyme of Streptococcus pyogenes, IdeS, is a cysteine protease produced and secreted extracellularly by the human pathogen Streptococcus pyogenes. This proteinase has extremely high substrate specificity, only recognizing IgG and cleaving at a specific site in the antibody's hinge region, resulting in the hydrolysis of IgG into intact F(ab’)2 fragments and Fc fragments. IdeS can recognize human and various other animal sources of IgG, such as human, rabbit, monkey, sheep, and human-animal chimeric IgG, etc. IdeS has unique substrate selectivity and can be used as a tool enzyme for structural characterization analysis of antibody drugs or antibody-fusion protein drugs.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product is recombinantly expressed in E. coli, with high purity and good enzymatic activity, making it a valuable tool for characterizing therapeutic antibodies, Fc fusion proteins, and antibody-drug conjugates.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHigh Specificity\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eSingle cleavage site: CPAPELLG\/GPSVF. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca name=\"OLE_LINK2\"\u003e\u003c\/a\u003e\u003cspan\u003eFast Reaction\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eminut\u003c\/span\u003e\u003cspan\u003ees\u003c\/span\u003e\u003cspan\u003e rapid cleavage, significantly faster than papaya proteinase and pepsin\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHigh Stability\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eEach batch of product undergoes strict quality control to ensure batch-to-batch stability. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSimple Reaction Conditions\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCompatible with various pH buffer solutions, no need for reducing agents or auxiliary reagents.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eIdeS enzyme is used as a tool enzyme in the preparation and structural characterization analysis of antibody drugs. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eIdeS enzyme can also assist in drug development in certain fields.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoNormalTable\" style=\"width: 99.9742%; height: 344.189px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 35.5957px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnglish synonym\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 35.5957px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eIdeS Protease\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 35.5957px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 35.5957px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ci\u003e\u003cspan\u003eE.coli \u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003erecombinant expression\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 35.5957px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLabel\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 35.5957px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eN-terminal His Tag\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 35.5957px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 35.5957px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity is greater than 95% as analyzed by SDS-PAGE and HPLC\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 35.5957px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 35.5957px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e35.3 KDa\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1953px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 55.1953px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 55.1953px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mM sodium phosphate, 150 mM NaCl (pH 6.6), 50% glycerol\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36.2207px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 36.2207px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnzyme Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 36.2207px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e U\/\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 74.7949px;\"\u003e\n\u003ctd style=\"width: 28.4926%; height: 74.7949px;\" valign=\"center\" width=\"155\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.5294%; height: 74.7949px;\" valign=\"center\" width=\"400\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe amount of enzyme required to cleave more than 95% of 1 μg of recombinant monoclonal IgG in 30 minutes at 37°C is defined as one unit of activity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"154\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"189\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"109\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20412ES84\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20412ES90\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"154\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e204\u003c\/span\u003e\u003cspan\u003e12\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"189\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eIdeS Protease\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"109\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e00 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e00 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eThe products \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003ecan\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e be stored at -\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e5℃ ~ -\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e5℃ for \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eone\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e year.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFigure\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_4d5cae5f-c36a-408d-b892-7f5701154cba_1024x1024.png?v=1732189555\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 1.  A. The HPLC result of MAG3-Cet-F(ab′)2 and MAG3-Cet-Fc after digesting MAG3-Cet with IdeS protease.  The HPLC result of the mixture from (A) but after reaction with protein A beads. The residual MAG3-Cet and most of the MAG3-Cet-Fc were removed by protein A beads.\u003c\/p\u003e\n\n\n\n\n\n\n\n\n\u003ch3 class=\"MsoNormal\"\u003e\u003cstrong\u003eRelated Reading\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/protein\/ides-protease-high-specificity-igg-degrading-enzyme?_pos=1\u0026amp;_sid=3b03df6fc\u0026amp;_ss=r\"\u003e\u003cspan\u003eIdeS Protease：High Specificity IgG Degrading Enzyme\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"2000 U","offer_id":53294149468525,"sku":"20412ES84","price":385.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294158250349,"sku":"20412ES90","price":685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/4_1024x1024_e992231a-66f9-4e30-bd4a-4f79c53ab6da.png?v=1778101062"},{"product_id":"recombinant-lys-c-ms-safe-expressed-in-e-coli-bhz20800031","title":"Recombinant Lys-C (MS-SAFE), Expressed in E.coli.","description":"\u003cp class=\"p\"\u003e\u003cspan\u003eLysine endopeptidase (Lys-C) is a serine protease derived from Pseudomonas species, which recognizes lysine in proteins and can specifically hydrolyze amide, ester, and peptide bonds on the carboxyl side of lysine. This product is a recombinant lysine endopeptidase expressed by Escherichia coli, free from other miscellaneous proteases (such as Lys-N, Arg-C, and other serine proteases), and has the same specificity and activity as natural lysine endopeptidase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eAdditionally, our company also provides recombinant trypsin (mass spectrometry grade) (Cat#20416ES), which cuts at the carboxyl end of lysine or arginine residues in proteins. Customers can choose according to their experimental needs.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e1.High activity\u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003egood stability\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.High purity of recombinant protein, free from other miscellaneous proteases, and no animal origin.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e1.Protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Synthesis of lysine-related derivatives.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eE.Coli recombinant expression\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e＞\u003c\/span\u003e95%\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e1.0 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSpecific activity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e≥3.0 AU\/mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSDS-PAGE\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eSingle main band\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e29±3 kDa\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e5 mM NaAc\u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e10 mM EDTA\u003c\/span\u003e\u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003epH=5.5\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eUnder the condition of 25 mmol\/L pH9.0 Tris-HCl buffer, at 37℃, the amount of enzyme required to catalyze the production of 1 μmol AC-Lys-pNA in 1 min is defined as one activity unit (AU)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe product can be stored at -25 ~ -15\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e℃ for \u003c\/span\u003eone years.\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 ug","offer_id":53294149632365,"sku":"20420ES20","price":165.0,"currency_code":"USD","in_stock":true},{"title":"5×20 ug","offer_id":53294158315885,"sku":"20420ES60","price":685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/universe_b9e9ebcb-b5e9-497a-b61d-dc198ade0f05.webp?v=1778112302"},{"product_id":"tte-uvrd-helicase-20-mu-g-ml-bhz20800101","title":"Tte UvrD Helicase (20 µg\/mL)","description":"\u003cp\u003eThe Tte UvrD Helicase, derived from the thermophilic bacterium \u003cem\u003eThermoanaerobacter tengcongensis\u003c\/em\u003e, possesses the ability to unwind double-stranded DNA under high-temperature conditions. This enzyme is stable at 65°C and can be applied in isothermal amplification techniques without relying on single-strand binding proteins. In thermophilic Helicase-Dependent Amplification (tHDA), it assists polymerase in DNA strand separation, enabling isothermal amplification.\u003c\/p\u003e\n\u003ch3\u003eProduct Features:\u003c\/h3\u003e\n\u003cp\u003e- Protein purity ≥95%;\u003cbr\u003e- Free of exonuclease, endonuclease, and RNase contamination;\u003cbr\u003e- Effectively eliminates non-specific amplification in LAMP systems.\u003c\/p\u003e\n\u003ch3\u003eFigure:\u003c\/h3\u003e\n\u003cp\u003eIn the LAMP system, it effectively eliminates non-specific amplification. Two sets of LAMP primers were used to prepare LAMP reaction systems, with each set including an experimental group supplemented with 10 ng Tte UvrD Helicase and a control group without it. \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/681d59b59932d6c9a36bb2bf.png?v=1751006621\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure1. Results showed that the addition of Tte UvrD Helicase effectively eliminated non-specific amplification in the LAMP system without affecting the detection of positive samples.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/681d59d99932d6c9a36bb2c0.png?v=1751006850\"\u003e\u003cbr\u003eFigure 2. Free of exonuclease, endonuclease, and RNase contamination.  20 ng of Tte UvrD Helicase was incubated with nucleic acid substrates, and band changes were analyzed via agarose gel electrophoresis. Experimental results showed that no exonuclease, endonuclease, or RNase contamination was detected in three batches of Tte UvrD Helicase.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"2 ug","offer_id":53294149566829,"sku":"14572ES05","price":235.0,"currency_code":"USD","in_stock":true},{"title":"10 ug","offer_id":53294158152045,"sku":"14572ES10","price":525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/681d59b59932d6c9a36bb2bf.png?v=1778101063"},{"product_id":"hifair-v-reverse-transcriptase-600-u-mu-l-glycerol-free-bhz20800077","title":"Hifair™ V Reverse Transcriptase (600 U\/μL, Glycerol free)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan\u003eHifair™\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eV Reverse Transcriptase is an updated version of Hieff™\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eM-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than Hieff™\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eM-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan\u003eV Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!--[endif]--\u003e\u003cspan\u003eOutstanding thermal resistance.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eSuitable for RT-PCR\/RT-qPCR applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGlycerol-free, more suitable for lyophilization.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan\u003ePerformance demonstration\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eCapable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_8efbd3fb-699c-493b-9c24-d517121cfc61.png?v=1733473526\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e\u003cspan\u003eFigure 1. The Reverse Transcriptase of Hifair™ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan\u003eV Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eV Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e2. The detection rate of RT-qPCR was better than that of other brands\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e,\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eand t\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003ehe detection range was 1 pg-1 μg\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_fc2124e9-d245-4653-93c6-1cfd9cab77bb.png?v=1733473545\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e\u003cspan\u003eFigure 2. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan\u003eV one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFAQ\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: What are the features of this product?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: M-MLV (H-) Reverse Transcriptase is produced by modifying the wild-type M-MLV reverse transcriptase gene and expressing and purifying it in Escherichia coli. It has no RNaseH activity and possesses the charact\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"3 KU","offer_id":53294149763437,"sku":"11301ES06","price":95.0,"currency_code":"USD","in_stock":true},{"title":"12 KU","offer_id":53294158676333,"sku":"11301ES12","price":315.0,"currency_code":"USD","in_stock":true},{"title":"120 KU","offer_id":53294158709101,"sku":"11301ES62","price":2375.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_d6036a34-a8d5-4def-8ad8-01bc7cf3b594.png?v=1778101062"},{"product_id":"ucf-me-rtev-protease-5-u-mu-l-bhz20800122","title":"UCF.ME™ rTEV Protease (5 U\/μL)","description":"\u003cp\u003eRecombinant TEV protease (rTEV Protease) is a recombinant protease modified and purified by genetic engineering, which not only maintains the functional activity of natural TEV Protease, but also shows stronger stability and specificity over a broad temperature range. rTEV Protease is a commonly tool enzyme for cutting affinity labels on fusion protein. It is specific to the seven amino acid sequence EXXYXQ↓(G\/S), and the cutting site is between glutamine and glycine\/serine. The commonly used seven amino acid sequence is Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly. rTEV Protease is optimally active at pH 7.0, 30°C, but is active over a wide range of pH 6.0-8.5 and temperatures 4-30°C (see Table 1), allowing the choice of reaction conditions to be flexible depending on the target protein. In addition, the 6×His label of N-terminal can also be used to remove rTEV Protease by Ni-NTA resin to purify the target protein.\u003c\/p\u003e\n\u003cp class=\"p\"\u003eThe product has been optimized on the basis of 20403ES, and compared with 20403ES, it has higher purity, stronger activity and lower \u003cem\u003eE.coli\u003c\/em\u003e gDNA residue, which is suitable for application with higher requirements for enzyme purity, activity and residue.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e\u003cstrong\u003eHigh cleavage efficiency\u003c\/strong\u003e: The protein tag is removed more thoroughly.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e\u003cstrong\u003eLow residual host gDNA\u003c\/strong\u003e: Effectively reduce the risk of introducing exogenous substance residues.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eRemoval of fusion tags\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cdiv align=\"center\"\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"212\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eUnit Definition\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"632\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eThe amount of enzyme required to cut \u0026gt;85% of the 3 \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eμ\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eg substrate in a 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003erTEV Buffer (50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT) at 30\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eo\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003efor 1 h was defined as a unit of activity\u003c\/span\u003e\u003c\/span\u003e\u003ci\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"212\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eProtein purity\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"632\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan\u003e≥\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003e95%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"212\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eSource\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"632\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eExpressed in \u003c\/span\u003e\u003c\/span\u003e\u003ci\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eE. coli\u003c\/span\u003e\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"187\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"300\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20427\u003c\/span\u003e\u003cspan\u003eES80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20427\u003c\/span\u003e\u003cspan\u003eES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20427\u003c\/span\u003e\u003cspan\u003eES50\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"187\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20427\u003c\/span\u003e\u003cspan\u003e-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"300\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003erTEV Protease (5 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"187\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20427\u003c\/span\u003e\u003cspan\u003e-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"300\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003erTEV Buffer (10×)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e600 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e6 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"133\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e30 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003erTEV Protease, stored at -25~-15\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eo\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003efor 6 months; Store at -85~-65\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eo\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003efor 1 year.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003erTEV Buffer, stored at -25~-15\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eo\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003efor 1 year.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNote: It is recommended to store each component separately when receiving the goods or using it for the first time to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20427-Fig_1.jpg?v=1737340009\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFigure\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e1. \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Calibri;\"\u003eVerification of Cleavage Activity of UCF.ME\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003csup\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Calibri;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003cspan style=\"font-family: Calibri;\"\u003erTEV Protease\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eNote: Using a fusion protein \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e(3 μg) c\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eontaining the UCF.ME\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003erTEV Protease cleavage site as the substrate, UCF.ME\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003erTEV Protease was added at 10, 5, and 3 U, respectively. The reaction was carried out at 30 \u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eo\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003efor 1 hour, and the cleavage effect was detected by agarose gel electrophoresis. The results showed that when the amount of UCF.ME\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003cspan style=\"font-family: Calibri;\"\u003erTEV Protease was more than 3 U, it could cleave the substrate efficiently with a cleavage efficiency of \u0026gt;90%.\u003c\/span\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 KU","offer_id":53294149861741,"sku":"20427ES10","price":665.0,"currency_code":"USD","in_stock":true},{"title":"50 KU","offer_id":53294156284269,"sku":"20427ES50","price":2905.0,"currency_code":"USD","in_stock":true},{"title":"1 KU","offer_id":53294156317037,"sku":"20427ES80","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20427-Fig_1.jpg?v=1778101061"},{"product_id":"3c-protease-expressed-in-e-coli-bhz20800003","title":"3C Protease, Expressed in E.coli.","description":"\u003cp\u003e\u003cspan\u003eLysine endopeptidase (Lys-C) is a serine protease derived from Pseudomonas species, which recognizes lysine in proteins and can specifically hydrolyze amide, ester, and peptide bonds on the carboxyl side of lysine. This product is a recombinant lysine endopeptidase expressed by Escherichia coli, free from other miscellaneous proteases (such as Lys-N, Arg-C, and other serine proteases), and has the same specificity and activity as natural lysine endopeptidase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdditionally, our company also provides recombinant trypsin (mass spectrometry grade) (Cat#20416ES), which cuts at the carboxyl end of lysine or arginine residues in proteins. Customers can choose according to their experimental needs.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003ch3\u003e\u003cspan\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e1.High activity\u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003egood stability\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.High purity of recombinant protein, free from other miscellaneous proteases, and no animal origin.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.Protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Synthesis of lysine-related derivatives.\u003c\/span\u003e\u003c\/p\u003e\n\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eE.Coli recombinant expression\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e＞\u003c\/span\u003e95%\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e1.0 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSpecific activity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e≥3.0 AU\/mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSDS-PAGE\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eSingle main band\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e29±3 kDa\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e5 mM NaAc\u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e10 mM EDTA\u003c\/span\u003e\u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003epH=5.5\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eUnder the condition of 25 mmol\/L pH9.0 Tris-HCl buffer, at 37℃, the amount of enzyme required to catalyze the production of 1 μmol AC-Lys-pNA in 1 min is defined as one activity unit (AU)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe product can be stored at -25 ~ -15\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e℃ for \u003c\/span\u003eone years.\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294149960045,"sku":"20409ES60","price":105.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294159561069,"sku":"20409ES76","price":305.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20409.webp?v=1778483438"},{"product_id":"hieff-unicon-hotstart-e-taq-dna-polymerase-50-u-mu-l-bhz20800071","title":"Hieff UNICON™ HotStart E-Taq DNA Polymerase, 50 U\/μL","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"p\"\u003e\u003cspan\u003eHieff UNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Hotstart E-Taq DNA Polymerase (Cat#1072\u003c\/span\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003eES) is a hot start DNA polymerase that employs the company's independently developed dual monoclonal antibodies for double closure. This product not only seals the 5'→3' polymerase activity of Taq DNA polymerase but also seals the 5'→3' exonuclease activity. Heating at the pre-denaturation temperature for 30 seconds can completely inactivate the closure antibodies, releasing the DNA polymerase activity and exonuclease activity. The dual closure feature not only effectively prevents non-specific amplification caused by mispairing or primer dimers but also effectively inhibits the decrease in fluorescence signals caused by probe degradation, providing dual protection to make in vitro diagnostic reagents more stable during transportation or use at room temperature. This Taq enzyme supports the development of a primer-probe pre-mixed system and offers higher sensitivity, specificity, and high GC sequence amplification capability compared to traditional qPCR reagents.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cspan class=\"16\"\u003eF\u003c\/span\u003e\u003cspan class=\"16\"\u003eeatures\u003c\/span\u003e\u003cspan class=\"16\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eHigh Specificity\u003c\/span\u003e\u003cspan\u003e: \u003c\/span\u003e\u003cspan\u003eCapable of dual encapsulation, it simultaneously inhibits the 5'-3' Taq DNA polymerase activity and the 5'-3' exonuclease activity at room temperature, enhancing the specificity of the reaction.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eNo Nuclease Contamination: Free from contamination of exonucleases, endonucleases, and RNases.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eSuitable for High-Sensitivity PCR Experiments: Exhibits a good linear relationship when the template concentration ranges from 5×10^6 copies\/T to 5×10^1 copies\/T, with the lowest detection limit reaching 5 copies\/T.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eSuperior Stability: When formulated into a complete premix containing primers and probes, it remains stable after being left at 37°C for 14 days and after 50 freeze-thaw cycles.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eCompatible with Rapid Amplification Protocols\u003cspan style=\"font-size: 0.875rem;\"\u003e: \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e45 cycles\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e, \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eresults within 30 minutes.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cspan class=\"16\"\u003eSpecifications\u003c\/span\u003e\u003cspan class=\"16\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"197\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePolymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTaq DNA Polymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"197\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e≥ 95%\u003c\/span\u003e\u003cspan\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eSDS-PAGE\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"197\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBuilt-In Hot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"197\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eReaction Speed\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStandard\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"197\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExonuclease Activity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5' - 3'\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cspan class=\"16\"\u003eComponents\u003c\/span\u003e\u003cspan class=\"16\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"295\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"136\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10727ES00\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e (500 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"136\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10727ES01\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e (5,000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"132\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10727ES94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(50,000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"295\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff UNICON® Hotstart Sensitive Taq DNA Polymerase (50 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"136\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"136\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"132\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"295\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"136\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10727ES80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294150058349,"sku":"10727ES00","price":100.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294154940781,"sku":"10727ES01","price":905.0,"currency_code":"USD","in_stock":true},{"title":"465000 U","offer_id":53294154973549,"sku":"10727ES80","price":46620.0,"currency_code":"USD","in_stock":true},{"title":"50000 U","offer_id":53294155006317,"sku":"10727ES94","price":7260.0,"currency_code":"USD","in_stock":true},{"title":"1250000 U","offer_id":53294155039085,"sku":"10727ES95","price":145185.0,"currency_code":"USD","in_stock":true},{"title":"5000000 U","offer_id":53294155071853,"sku":"10727ES96","price":391025.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/90267_d618f858-d3ad-4d8f-8f90-885558445f03.webp?v=1778484812"},{"product_id":"hieff-unicon-ucf-me-advanced-hotstart-taq-dna-polymerase-20-u-mu-l-bhz20800082","title":"Hieff UNICON UCF.ME™ Advanced HotStart Taq DNA Polymerase (20 U\/μL)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp\u003eThe product is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.\u003c\/p\u003e\n\u003cp\u003eIn addition, this product has been processed with the UCF.ME™ ultra-low residual process from Yeasen Biotechnology, resulting in extremely low residual levels of nucleases and host gDNA. With an optimized amplification buffer (e.g., Cat#16716ES), it can effectively reduce non-specific amplification caused by primer-probe hybridization during sample mixing, system warming, and long-term storage. This supports the development of a real time PCR system that allows for pre-mixing of primers and probes.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003eLow nuclease residue: No residual exonuclease, nicking enzyme, or RNase.\u003c\/li\u003e\n\u003cli\u003eUltra-low Residual: E. coli DNA residue level \u0026lt; 0.02 copies\/100 U, suitable for applications with more stringent background bacterial requirements.\u003c\/li\u003e\n\u003cli\u003eSuper stability: All components of the qPCR system except the template were combined and incubated at 37 °C for 7 days without compromising performance.\u003c\/li\u003e\n\u003cli\u003eBatch-to-batch stability: The UCF.ME™ ultra-clean molecular enzyme production platform, with strict quality control, ensures the uniformity, stability, and timely supply of the product.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\" style=\"width: 100%; height: 178.969px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"197\" valign=\"center\" style=\"width: 32.8333%; height: 35.5938px;\"\u003e\n\u003cp\u003ePolymerase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\" style=\"width: 85.5%; height: 35.5938px;\"\u003e\n\u003cp\u003eTaq DNA Polymerase\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36.5938px;\"\u003e\n\u003ctd width=\"197\" valign=\"center\" style=\"width: 32.8333%; height: 36.5938px;\"\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\" style=\"width: 85.5%; height: 36.5938px;\"\u003e\n\u003cp\u003e≥ 95%（SDS-PAGE）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"197\" valign=\"center\" style=\"width: 32.8333%; height: 35.5938px;\"\u003e\n\u003cp\u003eHot Start\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\" style=\"width: 85.5%; height: 35.5938px;\"\u003e\n\u003cp\u003eBuilt-In Hot Start\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"197\" valign=\"center\" style=\"width: 32.8333%; height: 35.5938px;\"\u003e\n\u003cp\u003eReaction Speed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\" style=\"width: 85.5%; height: 35.5938px;\"\u003e\n\u003cp\u003eStandard\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"197\" valign=\"center\" style=\"width: 32.8333%; height: 35.5938px;\"\u003e\n\u003cp\u003eExonuclease Activity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"513\" valign=\"center\" style=\"width: 85.5%; height: 35.5938px;\"\u003e\n\u003cp\u003e5' - 3'\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"220\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003eName\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"122\" valign=\"top\"\u003e\n\u003cp\u003e14321ES76\u003c\/p\u003e\n\u003cp\u003e (1,000 U)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"128\" valign=\"top\"\u003e\n\u003cp\u003e14321ES80\u003c\/p\u003e\n\u003cp\u003e (10,000 U)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"115\" valign=\"top\"\u003e\n\u003cp\u003e14321ES92\u003c\/p\u003e\n\u003cp\u003e(25,0\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 uL","offer_id":53294150091117,"sku":"14321ES76","price":335.0,"currency_code":"USD","in_stock":true},{"title":"500 uL","offer_id":53294155432301,"sku":"14321ES80","price":3225.0,"currency_code":"USD","in_stock":true},{"title":"1.25 mL","offer_id":53294155465069,"sku":"14321ES92","price":6425.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":53294155497837,"sku":"14321ES93","price":22875.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1024x1024_03941f37-67a8-4e15-aac9-6750be4cafee.png?v=1778101062"},{"product_id":"uracil-dna-glycosylase-udg-ung-1-u-mu-l-bhz20800052","title":"Uracil DNA Glycosylase (UDG\/UNG), 1 U\/μL","description":"\u003cdiv class=\"top_tabc proDetail\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eUDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e10 U of this product was detected by E.coli 16S rDNA-specific TaqMan qPCR, and the result showed that E.coli genome residue was fewer than 10 copies\u003cbr data-mce-fragment=\"1\"\u003eNo nucleic acid endonuclease, exonuclease and RNase residues\u003cbr data-mce-fragment=\"1\"\u003eUracil is the sole base recognized by this enzyme\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eRemove ssDNA and dsDNA, but they are inactive to RNA\u003cbr data-mce-fragment=\"1\"\u003eEliminate false positive results due to contamination of PCR amplification products.\u003cbr data-mce-fragment=\"1\"\u003eCompatible with PCR, qPCR, RT-qPCR, etc\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eSpecifications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\" data-mce-fragment=\"1\"\u003e\n\u003ctable cellspacing=\"0\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eExpression Host\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eRecombinant E. coli with uracil DNA glycosylase gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eMolecular Weight\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e24.8 kDa\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003ePurity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e≥ 95%（SDS-PAGE）\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eHeat Inactivation\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e95°C, 5~10 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eOne unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\" data-mce-fragment=\"1\"\u003e\n\u003ctable cellspacing=\"0\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e14455ES60 \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e(100 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e14455ES76 \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e(500 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e14455ES96 \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e(10,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e14455\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eUracil DNA Glycosylase (UDG\/UNG), 1 U\/μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e100 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e500 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eStorage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe product should be stored at -25℃ ~ -15℃ for two years.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg data-mce-fragment=\"1\" data-mce-src=\"https:\/\/seas.ysbuy.com\/file\/331887\/8682-167402383052946562.png\" src=\"https:\/\/seas.ysbuy.com\/file\/331887\/8682-167402383052946562.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eFigure 1．UDG enzyme (0.025 U) fully digested 360 ng of 200 bp dU -DNA\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eElectrophoresis results of 0.05 U, 0.025 U, 0.0125 U, 0.00625 U, 0.003125 U anti- contamination UDG enzyme with 360 ng of 200 bp dU -DNA incubated at 25°C for 30 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-mce-fragment=\"1\"\u003e\n\u003cspan class=\"boxTap\" data-mce-fragment=\"1\" id=\"tabCBox1\"\u003eDocuments:\u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"cwrap\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"list\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"title\" data-mce-fragment=\"1\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\u003ca class=\"down\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14455_Manual_HB230112_EN.pdf\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14455_Manual_HB230112_EN.pdf\" target=\"_blank\" title=\"download\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e14455_Manual_HB230112_EN.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294150156653,"sku":"14455ES60","price":55.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294158545261,"sku":"14455ES76","price":215.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294158578029,"sku":"14455ES96","price":1495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/8682-167402383052946562.png?v=1778101061"},{"product_id":"salt-active-ultranuclease-gmp-grade-bhz20800049","title":"Salt Active UltraNuclease GMP-grade","description":"\u003cp\u003eSalt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA \u0026amp; RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA \u0026amp; RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supernatant and cell lysate, increase purification efficiency in high salt condition.\u003c\/p\u003e\n\u003cp\u003eThe enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein\/polysaccharide pharmaceutical manufacturing.\u003c\/p\u003e\n\u003ch3 align=\"justify\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eHigh Activity in High-Salt Conditions:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Maintains optimal enzyme activity even at 500 mM salt concentration.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eEnhanced Viral Yield: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eHigh-salt tolerance reduces AAV particle aggregation, improving virus recovery.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eReliable Product Quality: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eGMP-grade, animal-free, and antibiotic-free.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eRegulatory Support:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e DMF filing completed (MF037815).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eHigh Purity: \u003c\/span\u003e\u003c\/b\u003e≥98% purity ensures consistent performance.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eLow Endotoxin: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eSuitable for sensitive applications.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eCompatible with ELISA Kits:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Fully supported for downstream ELISA-based analysis.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eExpression Host\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eRecombinant, expressed in\u003c\/span\u003e\u003ci\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/i\u003e\u003ci\u003e\u003cspan class=\"16\"\u003eEscherichia coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e24.7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ekDa\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eIsoelectric point\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e9.61\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e≥ 99%\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eEnzyme activity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e250-300 U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eSpecific activity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e≥3×10\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e U\/mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eProtein purity (SEC-HPLC)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e≥99%\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eBacterial\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eendotoxins(gel-clot\u003cspan style=\"font-family: Inter;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u0026lt;0.25 EU\/1000U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eHost\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eProtein\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eResidue(ELISA)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u0026lt;10 pp\u003c\/span\u003em(μg\/mL)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e37°C (operating range 0-42°C)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eCofactor\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1-10 mM Mg\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e25 mM Tris-HCl\u003cspan style=\"font-family: Inter;\"\u003e，\u003c\/span\u003e\u003c\/span\u003e5 mM MgCl\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e，\u003c\/span\u003e500 mM NaCl\u003cspan style=\"font-family: Inter;\"\u003e，\u003c\/span\u003e\u003c\/span\u003e50% glycerol\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"29.2400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"70.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eThe definition of one activity unit (U) is the amount of enzyme that causes a △A260=1.0 in 30 minutes\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eat 37℃ in the excess of substrate at the certain condition.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 align=\"justify\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eFigures\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cstrong\u003e1. Robust Activity in High-Salt Environments\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 1. Enzyme activity under high-salt conditions.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_0005f886-bb1e-41e8-95ef-a96e336bb514_1024x1024.png?v=1758782295\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cstrong\u003eFigure 1. Enzyme activity under high-salt conditions.\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e(A). UltraNuclease maintains robust activity in high-salt environments, showing maximum activity at 500 mM salt.\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e(B). Compared with Supplier M*, Yeasen UltraNuclease (Cat#20159ES) demonstrated lower HCD residuals, particularly under 400 mM NaCl with 1% Tween.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eEfficient Nucleic Acid Removal: AAV Production Case\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8600%\"\u003e\n\u003cp\u003e\u003cstrong\u003eKit\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"40.7000%\"\u003e\n\u003cp\u003e\u003cstrong\u003eCondition (100U\/mL, 37℃, 1 h)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.4000%\"\u003e\n\u003cp\u003e\u003cstrong\u003eHCD residuals（ng\/mL)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8600%\"\u003e\n\u003cp class=\"p\"\u003eSupplier\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eM*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"40.7000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e400mM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNaCl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.4000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1.42\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8600%\"\u003e\n\u003cp class=\"p\"\u003eSupplier\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eM*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"40.7000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e400mM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNaCl,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1%\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTween\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.4000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e0.68\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eYeasen 20159ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"40.7000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e400mM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNaCl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.4000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e0.87\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"25.8600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eYeasen\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e20159ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"40.7000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e400mM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNaCl,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1%\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTween\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"33.4000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e0.07\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eCompared with Supplier M*, Yeasen UltraNuclease (Cat#20159ES) demonstrated lower HCD residuals, particularly under 400 mM NaCl with 1% Tween.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eRecommended operating conditions\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"37.1200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eCondition\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"28.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eOptimal\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.1000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eEffective\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"37.1200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eMg\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\/Mn\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"28.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10~50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.1000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1~100\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"37.1200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003epH\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"28.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.1000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e6.5~10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"37.1200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eTemperature\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"28.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.1000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e4~42℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"37.1200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eNa\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\/K\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"28.7400%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.1000%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e300~1200\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e4. \u003c!--[endif]--\u003eRecommended Usage\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"53.3200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eCondition\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"46.6600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eSuggested usage\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"53.3200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eProtein\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"46.6600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU\/mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"53.3200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eCell lysate\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"46.6600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU\/mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"53.3200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eCell extract\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"46.6600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1000\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU\/mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"53.3200%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eViscosity reduction\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"46.6600%\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU\/mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e5. \u003c!--[endif]--\u003eStrong stability\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 2. Stability of UltraNuclease.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_ab801390-1b77-4801-84ef-1e0ccf2e5f91_1024x1024.png?v=1758782295\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cstrong\u003eFigure 2. Stability of UltraNuclease.\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(A) Enzyme activity remained unchanged after 4 weeks of storage at –20 °C and 4 °C, and after 2 weeks at 37 °C.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(B) Enzyme activity was unaffected after 10 freeze–thaw cycles between –80 °C and –25 °C.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe product should be stored at -25 ~ -15℃ for two years. If the product is opened and has been stored at 4℃ for more than one week, we recommend filtering the product to prevent microbial contamination.\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"25 KU","offer_id":53294150123885,"sku":"20159ES25","price":195.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294156972397,"sku":"20159ES60","price":585.0,"currency_code":"USD","in_stock":true},{"title":"1000 KU (4 mL)","offer_id":53294157005165,"sku":"20159ES80","price":2605.0,"currency_code":"USD","in_stock":true},{"title":"5000 KU (20 mL)","offer_id":53294157037933,"sku":"20159ES90","price":8805.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/2_1024x1024_c968bb41-c3ff-4c63-95f0-813dc0559387.png?v=1778101064"},{"product_id":"uracil-specific-excision-reagent-1-u-mu-l-bhz20800095","title":"Uracil-Specific Excision Reagent (1 U\/μL)","description":"\u003cdiv class=\"bhp-desc\" uracil-specific excision reagent to as user is an enzyme cocktail consisting of uracil dna glycosylase and endonuclease viii it capable generating single-nucleotide gaps specifically at locations. udg facilitates the cleavage base resulting in formation ap site while maintaining integrity phosphodiester sugar-phosphate backbone. activity endo cleaves bonds both ends apurinic thereby releasing deoxyribose sugar that lacking a base. residual nucleases or rnases high efficiency strand-specific library construction excision-mediated cloning assembly such tale monomers serial multiple cdna libraries from single cells u unit definition one defined amount required nick double-stranded oligonucleotide containing reaction system for minutes which corresponds pmol substrate. inactivation conditions min no. name buffer ml\u003e\n\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 U","offer_id":53294150222189,"sku":"14537ES50","price":105.0,"currency_code":"USD","in_stock":true},{"title":"250 U","offer_id":53294159724909,"sku":"14537ES72","price":405.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/8_8e21d841-ea48-4db2-a47d-147f2482e56a.png?v=1778101063"},{"product_id":"tth-dna-polymerase-5-u-mu-l-bhz20800102","title":"Tth DNA Polymerase (5 U\/μL)","description":"\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTth DNA Polymerase is a heat-resistant DNA polymerase discovered from the thermophilic bacterium \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eThermus thermophilus HB8\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e. In the presence of Mg²⁺, this enzyme exhibits 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity, but lacks 3′-5′ exonuclease activity, making it widely applicable for PCR amplification reactions. In the presence of Mn²⁺, the enzyme demonstrates strong reverse transcriptase activity at temperatures of 55-70°C, making it suitable for one-step RT-PCR reactions.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\" class=\"p\"\u003e\n\u003cspan\u003eSpecification\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eUnit Definition: \u003c\/span\u003e\u003cspan\u003eOne unit (U) of activity is defined as the amount of enzyme that incorporates 10 nmol of total nucleotides into an acid-insoluble product in 30 minutes at 74°C, using activated salmon sperm DNA as the template\/primer.  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\" class=\"p\"\u003e\n\u003cspan\u003eComponent\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" valign=\"center\" width=\"120\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eNo.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\" valign=\"center\" width=\"514\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\" valign=\"center\" width=\"480\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"201\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607ES72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e(250 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"278\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607ES80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e(1250 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"120\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"514\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTth DNA Polymerase (5 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"201\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"278\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"120\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"514\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e5×Tth RT-PCR Buffer \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"201\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"278\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e4×1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"120\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"514\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10×Tth PCR Buffer (with Mg2+)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"201\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"278\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2×1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"120\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607-D\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"514\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e50mM Mg(OAc)2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"201\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"278\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"120\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14607-E\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"514\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003edNTP Mixture (10 mM each)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"201\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e150 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"278\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e750 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 align=\"justify\" class=\"p\"\u003e\n\u003cspan\u003eShipping\u003c\/span\u003e\u003cspan\u003e and Storage \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003cspan\u003ewith dry ice. Store\u003c\/span\u003e\u003cspan\u003e at\u003c\/span\u003e\u003cspan\u003e -20°C, with a shelf life of 2 years.  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\" class=\"p\"\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ePrecautions  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1) If a small amount of precipitate appears in the buffer upon thawing, this is a normal phenomenon. Please invert and mix thoroughly before use.  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2) For your safety and health, wear a lab coat and disposable gloves during operation.  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e3) This product is for research use only!  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\" class=\"p\"\u003e\n\u003cspan\u003eInstructions  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eRecommended PCR Reaction Procedure:  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1. Preparation of the Reaction System \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eVolume (\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e                     \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10×Tth PCR Buffer (with Mg2+)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTth DNA Polymerase (5 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e0.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003edNTP Mixture (10 mM each)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eUpstream\u003c\/span\u003e\u003cspan\u003e Primer 10μM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eDownstream\u003c\/span\u003e\u003cspan\u003e Primer 10μM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTemplate DNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003epg-\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1μg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"564\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eddH\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"552\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003eto 50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e[Note]: The amounts of Tth DNA Polymerase, upstream and downstream primers, and dNTP Mixture can be adjusted according to specific experimental needs.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2. Cycle Setup\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eSteps\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"188\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTemp(\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eºC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"268\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTime\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"306\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eCycles\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003ePre-denaturation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"188\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"268\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 sec-5 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"306\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eDenaturation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"188\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"268\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 sec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"3\" valign=\"center\" width=\"306\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e35\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eAnnealing\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"188\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e50-70\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"268\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 sec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eExtension\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"188\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"268\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e60 sec\/kb\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eFinal Extension\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"188\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"268\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"306\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e[Note]: Reaction conditions can be adjusted according to specific experimental requirements.  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eRecommended RT-PCR Reaction Procedure:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1. Preparation of the Reaction System\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eVolume (\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e   \u003c\/span\u003e\u003cspan\u003e                    \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e5×Tth RT-PCR Buffer \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTth DNA Polymerase (5 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e50mM Mg(OAc)2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003edNTP Mixture (10 mM each)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1.5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eUpstream \u003c\/span\u003e\u003cspan\u003ePrimer 10μM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eDownstream\u003c\/span\u003e\u003cspan\u003e Primer 10μM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTemplate RNA \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e50 pg- 1μg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"716\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eddH2O\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"369\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eto 50 \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e[Note]: The amounts of Tth DNA Polymerase, upstream and downstream primers, and dNTP Mixture can be adjusted according to specific experimental needs.  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2. Cycle Setup\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eSteps\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTemp (\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eºC\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eTime\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"255\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eCycles\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eReverse transcription\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e55-70\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"255\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003ePre-\u003c\/span\u003e\u003cspan\u003edenaturation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 sec-5 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"255\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eDenaturation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 sec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"3\" valign=\"center\" width=\"255\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e35\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eAnnealing\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e50-70\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e30 sec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eExtension\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e60 sec\/kb\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"346\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eFinal Extension\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"276\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e72\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"205\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"255\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e[Note]: Reaction conditions can be adjusted according to specific experimental requirements.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"250 U","offer_id":53294150254957,"sku":"14607ES72","price":95.0,"currency_code":"USD","in_stock":true},{"title":"1250 U","offer_id":53294155333997,"sku":"14607ES80","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Univ_944de0c5-6e57-417d-a28e-c450ce5bc1fd.webp?v=1778482539"},{"product_id":"mrna-vaccinia-capping-enzyme-gmp-grade-10-u-mu-l-bhz20800065","title":"mRNA Vaccinia Capping Enzyme GMP-grade (10 U\/μL)","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eEukaryotes mRNA forms a special structure at the 5'end after transcription, which is the cap structure. The cap structure plays an important role in the stability, transportation, and translation of mRNA. The vaccinia virus capping enzyme is an effective enzyme that can catalyze the formation of the cap structure. It\"s composed of two subunits D1 and D12. It also has RNA triphosphatase activity, guanylate acyltransferase activity, and guanine methyltransferase activity that could connect the 7-methylguanine cap structure (m7Gppp) to the 5'end of the RNA (m7Gppp5'N). Vaccinia virus capping enzyme can cap the RNA in correct direction within one hour when present at a suitable concentration of capping buffer, guanosine triphosphate (GTP), S-adenosylmethionine (SAM), etc.\u003cbr\u003eThis product is produced by GMP process requirements and provided in a liquid form, used for in vivo\/in vitro pre-translation mRNA capping reaction or mRNA 5'end labeling reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsolated from a recombinant source\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eCapping mRNA before translation assays\/in vitro translation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLabeling 5´ end of mRNA\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant E. coli with vaccinia virus capping enzyme gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerin\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 unit: The amount of enzyme required to incorporate 10 pmol GTP (α- 32P) into a transcript with 80 nucleotides (80 nt) at 37℃ within 1 hour. \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES84 (2,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES92 (10,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES96 (100,000 U)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10614ES99 (5 MU)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10614\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003emRNA Vaccinia Capping Enzyme GMP-grade (10 U\/μL)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e500 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\n\u003cstrong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003emRNA Vaccinia Capping Enzyme GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/759158\/19c5-167031123115462375.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. The capping efficiency almost reached 100% by the vaccinia capping system from YEASEN. Nucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\"\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\"\u003eDocuments:\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\"\u003e\u003cspan class=\"boxTap\"\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"cwrap\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv\u003e\u003ca class=\"down\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/MSDS-10614-mRNA%20Vaccinia%20Capping%20Enzyme%20GMP-grade%20(10%20U_%CE%BCL)-HB220701.pdf\" target=\"_blank\" title=\"download\"\u003e\n\u003cp\u003eMSDS-10614-mRNA Vaccinia Capping Enzyme GMP-grade (10 U_μL)-HB220701.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"title\"\u003eManuals\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"2 KU","offer_id":53294150320493,"sku":"10614ES84","price":365.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":53294157234541,"sku":"10614ES92","price":1445.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/19c5-167031123115462375.png?v=1778101063"},{"product_id":"ucf-me-advanced-uracil-dna-glycosylase-udg-ung-low-dna-10-u-mu-l-bhz20800090","title":"UCF.ME™ Advanced Uracil DNA Glycosylase (UDG\/UNG), Low DNA, 10 U\/μL","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA, it has no catalytic activity in RNA.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product has been processed by the UCF.ME™\u003c\/span\u003e\u003cspan\u003e ultra-low residue process, and its residual DNA contamination of \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e is extremely low, which is suitable for applications with stricter requirements for background bacteria and higher performance demands, such as the development of real-time quantitative PCR systems that support the pre-mixing of primers and probes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeatures \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003eUltra-low residual of UCF.ME™ \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli \u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003egenomic DNA residue \u0026lt;0.1 copies\/ 100 U;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003eLow nuclease residue\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eNo residual exonuclease, nicking enzyme, or RNase;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003eStrong digestion capability\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0.05 U\/T can digest 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e copies\/T dU-DNA products;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003eCompatible with RT-qPCR reaction systems\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eNo impact on the detection system even at high input levels (2 U\/20 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eμ\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eL reaction system).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003eSuper stability: All components of the qPCR system except the template were combined and incubated at 37 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e°\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eC for 7 days without compromising performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003ca name=\"_Hlk146058318\"\u003e\u003c\/a\u003e\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cdiv align=\"center\"\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"518\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRecombinant \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e with UDG gene from psychrophilic marine bacteria\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"518\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20 mM Tris-HCl, 100 mM KCl, 1 mM EDTA, 60% Glycerol, 1 mM DTT, pH 8.0\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e±\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e0.2@25\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"518\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOne unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"175\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHeat Inactivation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"518\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e95\u003c\/span\u003e℃ for \u003cspan\u003e5~10 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30.3600%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"17.3400%\" valign=\"top\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294150287725,"sku":"14468ES60","price":595.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294156579181,"sku":"14468ES76","price":1055.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/12316_4e24e060-f97f-4455-af15-a443bfde676b.webp?v=1778482877"},{"product_id":"uracil-dna-glycosylase-udg-heat-labile-glycerol-free-1-u-mu-l-bhz20800051","title":"Uracil DNA Glycosylase (UDG), Heat-Labile (Glycerol free) (1 U\/μL)","description":"\u003cp\u003eUDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP.  Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature. \u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003eApplications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e1. Remove aerosol contamination from PCR products containing dU.\u003cbr\u003e\u003cbr\u003e2. Remove single-stranded or double-stranded DNA uracil bases.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eUnit Definition\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThe amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDeactivation method\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e94℃, 2-5 minutes.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\n\u003cstrong\u003eComponents\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"76\"\u003e\n\u003cp\u003eNo.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"291\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"159\"\u003e\n\u003cp\u003e10707ES60\u003c\/p\u003e\n\u003cp\u003e(100 U)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003e10707ES76\u003c\/p\u003e\n\u003cp\u003e(500 U)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"159\"\u003e\n\u003cp\u003e10707ES90\u003c\/p\u003e\n\u003cp\u003e(5 KU)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"244\"\u003e\n\u003cp\u003e10707ES92\u003c\/p\u003e\n\u003cp\u003e(10 KU)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"76\"\u003e\n\u003cp\u003e10707\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"291\"\u003e\n\u003cp\u003eUracil DNA Glycosylase (UDG, Glyceol-free), (1 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"159\"\u003e\n\u003cp\u003e100 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"175\"\u003e\n\u003cp\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"159\"\u003e\n\u003cp\u003e5 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"244\"\u003e\n\u003cp\u003e10 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003e\n\u003cbr\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003cp\u003eIce pack transport. Store at 4°C, valid for 6 months.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003ePrecautions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003col\u003e\n\u003cli class=\"tableResponsive\"\u003eFor your safety and health, please wear a lab coat and disposable gloves.\u003c\/li\u003e\n\u003cli class=\"tableResponsive\"\u003eThermosensitive UDG is active in most PCR reaction buffers.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294150451565,"sku":"10707ES76","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10303.webp?v=1778110789"},{"product_id":"rnase-r-gmp-grade-20-u-mu-l-bhz20800038","title":"RNase R (GMP-grade, 20 U\/μL)","description":"\u003cp style=\"font-weight: 400;\"\u003eRibonuclease R (RNase R) from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e, is a magnesium-dependent 3′→5′ exoribonuclease that\u003cspan\u003e \u003c\/span\u003ecan digest all linear RNAs but does not digest lariat or circular RNA structures. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA and intron lariats. RNase R is used for enrichment of circRNAs in biological samples, identification of intronic lariat sequences, identification of exonic circRNAs, studying alternative splicing.\u003c\/p\u003e\n\u003carticle class=\"4ever-article\"\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003eRNase R must be used in combination with the 10× RNase R Reaction Buffer, GMP-grade (Cat. No. 14616ES)\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/article\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eFeatures  \u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eGuaranteed efficacy and quality with ISO 13485 certification.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eAvailable in bulk or tailored configurations.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eEffectively breaks down linear RNA, while preserving lariat loops and circular RNAs.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSelective digestion of linear RNAs enhances the isolation of circular RNAs for protein production or intronic cDNA library construction.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eOffering GMP-Grade RNAse R\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eSpecificntions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eA recombinant \u003cem\u003eE. coli\u003c\/em\u003e strain carrying the RNAse R gene from \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl,100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, 1 mM DTT, 50% glycerol，pH7.5 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl\u003csub\u003e2\u003c\/sub\u003e.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R serves as a fundamental component in circular RNA synthesis (circRNA). CircRNA holds promise for enhancing pharmaceutical stability and biostability. With their covalently closed structures, circRNAs withstand RNA exonucleases. [1,2] RNase R finds widespread application in various studies, including alternative splicing, intron cDNA production, and the isolation of splicing intermediates and lariats.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong data-filtered=\"filtered\"\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES25\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES72\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e14615\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R (GMP-grade, 20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e25 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e250 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003ePrepare the following reaction system in a sterile microcentrifuge tube\u003c\/p\u003e\n\u003ctable style=\"font-weight: 400;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003ecomponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eVolume\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10×RNase R Reaction Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNA sample\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1\u003cspan\u003e \u003c\/span\u003eμg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase R (20 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2-4 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eUp to 20\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIncubate at 37 ℃ for 10 - 30 minutes.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\n\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-1_600x600.jpg?v=1744210829\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 1: Total mRNA (C) can be digested by Yeasen RNAse R. Total mRNA input: 500ng. \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-2_600x600.jpg?v=1744210830\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 2: CircRNA can not be digested by Yeasen RNAse R. CircRNA input: 500ng, RNAse R: 4U. \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14615-3_1024x1024.jpg?v=1744210830\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003eFigure 3: Performence of Yeasen RNAse R  (1U to 8U ) digestion on 1 μg of linear mRNA (C).\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThe activity of RNase R requires 0.1-1.0 mM Mg\u003csup\u003e2+\u003c\/sup\u003e;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eWith the increase of RNA, the reaction time can be extended and the enzyme amount can be increased appropriately;\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThe EDTA content in RNA samples may affect the activity of RNase R.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eIncubate at 70 ℃ for 10 minutes can inactivate the enzyme.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294150517101,"sku":"14615ES25","price":205.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294155596141,"sku":"14615ES72","price":1685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/14615-1_600x600_2b00ec45-e878-4f09-af12-17359ab82dd3.jpg?v=1778101060"},{"product_id":"hieff-unicon-hotstart-taq-dna-polymerase-50-u-mu-l-bhz20800073","title":"Hieff UNICON™ HotStart Taq DNA Polymerase, 50 U\/μL","description":"\u003cp align=\"justify\" class=\"17\"\u003e\u003cspan\u003eHieff\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e HotStart Taq DNA Polymerase is a mixture of Hieff\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq Antibody\u003cspan\u003e(\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eCatalog #31301ES\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eand Hieff\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Taq DNA Polymerase. Hieff\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTaq Antibody had a high affinity for Hieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTaq DNA Polymerase, and the activity of Hieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTaq DNA Polymerase could still be blocked after being treated at 50℃ for 30 min. The product was completely inactivated by heating at pre denaturation temperature for 30 seconds, releasing DNA polymerase activity. The hot start Taq enzyme can effectively inhibit the amplification caused by non-specific annealing of primers.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"17\"\u003e\u003cspan\u003eHieff\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eHotStart Taq DNA Polymerase is suitable for hot start PCR and qPCR. Hieff\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTaq DNA Polymerase in this product is a heat stable recombinant DNA polymerase with high template affinity, which is very suitable for amplification of low copy template. The amplified product has 3 '- dA and can be easily cloned into T vector.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eF\u003c\/span\u003e\u003cspan class=\"15\"\u003ee\u003c\/span\u003e\u003cspan class=\"15\"\u003eatures\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eClosed Detection of DNA Polymerase Activity\u003cspan\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eIn the Hieff\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUNICON\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eHotStart Taq Buffer (Mg2+  Plus) \u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003ethe activity release was less than 5% after incubation at 65℃ for 30 min.\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eDNA Polymerase Activity Release Detection \u003cspan\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eIn the HieffUNICON\u003c\/span\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eHotStart Taq Buffer (Mg2+  Plus) \u003cspan\u003e，\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003ethe activity release was higher than 95% when heated at 95℃ for 30 sec.\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eExonuclease Activity: In 20 μL reaction system, 10 U of this product and 0.6 μg of λ - HindIII were incubated at 74℃ for 1 h, and the electrophoresis band of DNA had no change.\u003c\/li\u003e\n\u003cli\u003eEndonuclease Activity: In 20 μL reaction system, 10 U of the product and 0.6 μg of supercoiled pBR322 DNA were incubated at 74 ℃ for 1 h, and the DNA electrophoretic bands did not change.\u003c\/li\u003e\n\u003cli\u003eResidual \u003ci style=\"font-size: 0.875rem;\"\u003eE.coli\u003c\/i\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e gDNA Test: The E.coil 16S rDNA gene was amplified by adding 2 U of the product into 50 μL system and using\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003esterile ddH2O as template. After 35 cycles, the amplified products were subjected to 1% agarose gel electrophoresis and EB staining without amplification bands.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eSpecifications \u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePolymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTaq DNA Polymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e≥ 95%\u003c\/span\u003e\u003cspan\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eSDS-PAGE\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBuilt-In Hot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eReaction Speed\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStandard\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eExonuclease Activity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5' - 3'\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"197\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"513\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUsing the activated DNA of salmon sperm as template \/ primer, the activity was defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"268\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"117\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10730\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e00\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e (\u003c\/span\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e0 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"125\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10730\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e01\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e (5\u003c\/span\u003e\u003cspan\u003e,0\u003c\/span\u003e\u003cspan\u003e00 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"116\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10730\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e03\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003e50,\u003c\/span\u003e\u003cspan\u003e000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"129\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10730\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(1\u003c\/span\u003e\u003cspan\u003e,250,\u003c\/span\u003e\u003cspan\u003e000 U)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"268\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHotStart Taq DNA Polymerase (5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e U\/μL)  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"117\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"125\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"116\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"129\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eStorage\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for \u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294150582637,"sku":"10730ES00","price":100.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294155235693,"sku":"10730ES01","price":905.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/90267_83e07915-2272-42ad-a064-1738cd3cb637.webp?v=1778484757"},{"product_id":"recombinant-trypsin-ms-safe-expressed-in-yeast-gmp-grade-bhz20800032","title":"Recombinant Trypsin (MS-SAFE), Expressed in Yeast, GMP-grade","description":"\u003cp class=\"p\"\u003e\u003cspan\u003eTrypsin is a serine protease secreted by the pancreas, which recognizes the sites of lysine or arginine in proteins and cuts at the carboxyl end of the lysine or arginine residues. This product is a recombinant porcine trypsin expressed by Pichia pastoris, produced under GMP regulations, free of any animal-derived components, and free from animal virus contamination (such as swine influenza virus, porcine parvovirus, etc.). It naturally lacks chymotrypsin activity and has been filtered to remove bacteria. The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, and metal ion chelating agents such as EDTA.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eYeasen Recombinant Trypsin activity is based on the internationally accepted activity (USP) and is superior to imported similar products. The HPLC content of β-Trypsin can reach over 80%, with high storage stability and a proteomics enzymatic digestion miss-cut rate that is 50% lower than imported products.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1.High activity\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e，\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003egood stability\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e2.Free of any protease inhibitors\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e，\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003esuch as PMSF\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eProtein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eYeast recombinant expression\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan\u003e＞\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e2.0 mg\/mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eSpecific activity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan\u003e＞\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e6800 USP Unit\/mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eβ-Trypsin\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan\u003e＞\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e80%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eα-Trypsin\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan\u003e＜\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e20%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e1 mM HCL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"305\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"1089\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eAt 25°C, pH 7.6, in a reaction system of 3.2 mL (1 cm light path), the increase in absorbance at 253 nm by 0.003 per minute during the enzymatic digestion of BAEE is defined as one USP unit.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eThe product can be stored at -25 ~ -15\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003e℃ for \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003etwo years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 ug","offer_id":53294150615405,"sku":"20416ES60","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/universe_d3fee606-3b45-4167-a7a6-6e69b4735923.webp?v=1778482906"},{"product_id":"mrna-cap-2-o-methyltransferase-gmp-grade-50-u-mu-l-bhz20800064","title":"mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U\/μL)","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003emRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.\u003cbr\u003eThis product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo\/in vitro.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsolated from a recombinant source\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTested for the absence of endonucleases, exonucleases, Rnases\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003emRNA cap-1 structure may improve expression\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRecombinant E.coli with Cap 2´-O-Methyltransferase gene\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimum Temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e20 mM Tris-HCl pH8.0, 0.1mM EDTA, 1mM DTT, 100mM NaCl, 50% (v\/v) glycerin, 0.1% (v\/v) Trion X-100  \u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eOne unit is defined as the amount of enzyme required to methylate 10 pmol of 80nt capped RNA transcript in 1 h at 37°C.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES92\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(10,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES97\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(50,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES98\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(250,000 U)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003e10612ES99\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(20 MU)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10612\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003emRNA Cap 2´-O-Methyltransferase GMP-grade (50 U\/μl)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e400 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe mRNA Cap 2´-O-Methyltransferase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/369399\/6593-167030752734177646.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigures 1. The relationship between capping efficiency and 2’-O-Methyltransferase quantity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox1\"\u003eDocuments:\u003c\/span\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"cwrap\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"list\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"title\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv\u003e\u003ca class=\"down\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/MSDS-10612-mRNA%20Cap%202%C2%B4-O-Methyltransferase%20GMP-grade%20(50%20U_%CE%BCl)-HB220701.pdf\" target=\"_blank\" title=\"download\"\u003e\n\u003cp\u003eMSDS-10612-mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U_μl)-HB220701.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"title\"\u003eManuals\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10000 U","offer_id":53294150680941,"sku":"10612ES92","price":165.0,"currency_code":"USD","in_stock":true},{"title":"50000 U","offer_id":53294158381421,"sku":"10612ES97","price":685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/6593-167030752734177646.png?v=1778101061"},{"product_id":"ucf-me-ultranuclease-bhz20800120","title":"UCF.ME™ UltraNuclease","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eUltraNuclease, also known as non-specific nuclease or broad-spectrum nuclease, is a non-specific endonuclease derived from Serratia Marcescen. It can cleave at any nucleotide within the chain, completely digesting nucleic acids into 5'-monophosphate oligonucleotides of 2-5 bases in length. UltraNuclease is capable of degrading various forms (double-stranded, single-stranded, linear, circular, native, or denatured) of DNA and RNA under a wide range of conditions (6 M Urea, 0.1 M Guanidine HCl, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF). It is widely used for removing nucleic acids from biological products. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis product is genetically engineered and expressed in\u003c\/span\u003e\u003ci\u003e\u003cspan\u003e Escherichia coli (E. coli)\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e, with a purity ≥99%. It is used to reduce the viscosity of cell supernatants and cell lysates, improve protein purification efficiency and functionality studies, and effectively prevent the clumping of human peripheral blood mononuclear cells (PBMCs) in cell therapy and vaccine research.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eIt is provided as a sterile liquid enzyme stored in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 20 mM NaCl, 50% Glycerol), appearing as a clear, colorless liquid.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeature\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003es\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003eWide range of applications: degrade all forms of DNA and RNA\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHigh purity and activity: purity ≥ 99%; specific activity ≥ 1.5×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e U\/mg\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\" style=\"width: 99.9635%; height: 515.249px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"top\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"top\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ci\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMolecular Weight\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e26.5 kDa\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp\u003eIsoelectric Point\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp\u003e6.85\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp\u003e≥ 99%（HPLC）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp\u003eEnzyme Activity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp\u003e250-300 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2523px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 39.2523px;\"\u003e\n\u003cp\u003eSpecific Activity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 39.2523px;\"\u003e\n\u003cp\u003e≥1.5×\u003cspan\u003e10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/sup\u003e U\/mg protein\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp\u003eOptimum pH\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp\u003e8.0 (pH range 6-10)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp\u003eOptimum Temperature\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp\u003e37°C (temperature range 0-42°C)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5919px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 35.5919px;\"\u003e\n\u003cp\u003eCofactor\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 35.5919px;\"\u003e\n\u003cp\u003e1-10 mM Mg2+\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1869px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\" style=\"width: 34.4144%; height: 55.1869px;\"\u003e\n\u003cp\u003eStorage Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"612\" valign=\"center\" style=\"width: 110.27%; height: 55.1869px;\"\u003e\n\u003cp\u003e20 mM Tris-HCl pH8.0，2 mM MgCl2，20 mM NaCl，50% Glycerol\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 136.075px;\"\u003e\n\u003ctd width=\"191\" valign=\"center\"\u003e\n\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"25 KU","offer_id":53294150713709,"sku":"20156ES25","price":155.0,"currency_code":"USD","in_stock":true},{"title":"50 KU","offer_id":53294157332845,"sku":"20156ES50","price":255.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294157365613,"sku":"20156ES60","price":355.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/27027821947a49209e054ebf10b9cf65_1024x1024_a1afa4cb-8b01-4543-bd1d-f336c3ba297c.png?v=1778101063"},{"product_id":"sumo-protease-1-u-mu-l-expressed-in-e-coli-bhz20800040","title":"SUMO Protease (1 U\/μL, Expressed in E.coli.)","description":"\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eSUMO protease recognizes the complete SUMO (Small Ubiquitin-like Modifier) label protein with 100 amino acids and efficiently cuts the SUMO from the fusion protein. Compared with the recognition sites of EK and TEV, SUMO protease has high specificity due to its long recognition sequence, and maintains high activity in a wide range of reaction environmental systems, such as temperature (4-30℃), pH (5.5-9.5), etc. SUMO protease also has a polymerized His label, which is convenient to remove SUMO protease and purify the target protein by affinity chromatography.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eCat.No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410ES60 \/ 20410ES70 \/ 20410ES80\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e100 U \/ 500 U \/ 1000 U\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e1 U\/μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eThe amount of enzyme required to cut \u0026gt;85% of the 3 μg substrate in a 1×rTEV Buffer (50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT) at 30℃ for 1 h was defined as a unit of activity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eExpressed in \u003ci\u003eE. coli\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eLabel\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003ePolymerized His label\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eProtein purity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e\u0026gt; 90%\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eMolecular weight\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e26.55 kDa\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.7624%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75.9048%;\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e25 mM Tris-HCl, pH 8.0\u003cspan\u003e、\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e0.1% Igepal (NP-40)\u003c\/span\u003e\u003cspan\u003e、\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e250 mM NaCl\u003c\/span\u003e\u003cspan\u003e、\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e500 μM DTT\u003c\/span\u003e\u003cspan\u003e、\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e50% glycerin\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\" style=\"width: 100.061%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 100.062%;\" valign=\"top\"\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410ES60\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410ES70\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410ES80\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eSUMO Protease (1U\/μL)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410-B1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e10×Protease Buffer (-salt)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e20410-B2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e10×Protease Buffer(+salt)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003eNote\u003cspan\u003e：\u003c\/span\u003eThe enzyme activity definition uses 10×Protease Buffer (-salt). SUMO Protease has relatively high activity in 10×Protease Buffer (-salt), but for unstable substrates, 10×Protease Buffer (+salt) should be selected. We also provide reaction buffer 20410-B2 with +salt. Choose the correct buffer for your experiment. 20410-B2\u003cspan\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e500 mM Tris-HCl, pH 8.0\u003c\/span\u003e, 2% Igepal (NP-40), 1.5 M NaCl, 10 mM DTT\u003cspan\u003e；\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e20410-B1\u003c\/span\u003e\u003cspan\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e500 mM Tris-HCl, pH 8.0\u003c\/span\u003e, 2% Igepal (NP-40), 10 mM DTT.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 100.062%;\" valign=\"top\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThis product should be stored at -85~-65℃ for 1 year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eThe following reaction system is formulated in the EP tube:\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eIncubate at 30℃, suck out 20 μL of the above reaction solution at 1, 2, 4 and 6 hours in a separate EP tube respectively.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAdd 20 μL of 2×SDS loading Buffer into the EP tube and place it at -20℃.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAfter the reaction of all samples was completed, the samples were boiled for 5 min and 30 μL was taken for SDS-PAGE analysis. Determine the optimal response time.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable align=\"center\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eVolume (μL) (The reaction system can be scaled down equally)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eFusion protein\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e50 μg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003e10×Protease Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e20 \u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eSUMO Protease (1U\/μL)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cspan\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eto 200 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003eNote: If the fusion protein requires low temperature treatment, the reaction solution can be placed at 4℃ to prolong the reaction time and increase the amount of SUMO enzyme to ensure the effect of enzyme digestion.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eTable 1. SUMO Protease digestion activity at different temperatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable align=\"center\" class=\"Table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003eIncubation Time\u003cspan\u003e（\u003c\/span\u003eh\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"4\" valign=\"center\"\u003e\n\u003cp align=\"center\"\u003e\u003cspan\u003eDigestion activity at different temperatures (%)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e4℃\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e16℃\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e25\u003cspan style=\"font-family: Times New Roman, Times, serif;\"\u003e℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e30℃\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e0.5\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e48\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e73\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e83\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e88\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e60\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e87\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e90\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e93\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e71\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e94\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e94\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e95\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e74\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e95\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e95\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp\u003e\u003cspan\u003e95\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e \u003cspan\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. This product is for research use only.\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294150877549,"sku":"20410ES60","price":155.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294156448109,"sku":"20410ES76","price":695.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20410.webp?v=1778112170"},{"product_id":"recombinant-bovine-enterokinase-his-expressed-in-yeast-5-u-mu-l-bhz20800026","title":"Recombinant Bovine Enterokinase, His, Expressed in Yeast (5 U\/μL)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRecombinant \u003c\/span\u003e\u003cspan\u003eE\u003c\/span\u003e\u003cspan\u003enterokinase\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis a high-purity recombinant fragment of bovine enterokinase light chain, with an amino acid sequence identical to that of the bovine enterokinase light chain. It has the same specific cleavage site as naturally extracted enterokinase, which is Asp-Asp-Asp-Asp-Lys (DDDDK). It can be used to remove fusion proteins located at the N-terminus of proteins to remove unwanted fusion tags, ensuring the accurate N-terminal sequence of recombinant fusion proteins. At the same time, Recombinant \u003c\/span\u003e\u003cspan\u003eE\u003c\/span\u003e\u003cspan\u003enterokinase has higher cutting activity than natural enzymes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eR\u003c\/span\u003e\u003cspan\u003eecombinant\u003c\/span\u003e\u003cspan\u003e E\u003c\/span\u003e\u003cspan\u003enterokinase\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003eHis\u003c\/span\u003e\u003cspan\u003e Tag \u003c\/span\u003e\u003cspan\u003eis a high-purity, high-activity, and highly specific bovine enterokinase expressed by Pichia pastoris secretion. It can effectively cleave fusion proteins within a wide pH range (4.5-9.5) and a wide temperature range, and still has partial activity under various detergents and denaturants. This product has a His tag, which can be easily removed through Ni\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e affinity column after the cutting reaction, greatly simplifying the subsequent purification process.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eStrong specificity\u003c\/strong\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eA specific protease\u003c\/span\u003e\u003cspan\u003e that \u003c\/span\u003e\u003cspan\u003ecut\u003c\/span\u003e\u003cspan\u003es the\u003c\/span\u003e\u003cspan\u003e carboxyl termi\u003c\/span\u003e\u003cspan\u003enus\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eof lysine\u003c\/span\u003e\u003cspan\u003e contain\u003c\/span\u003e\u003cspan\u003eing\u003c\/span\u003e\u003cspan\u003e four aspartic acid\u003c\/span\u003e\u003cspan\u003es in front\u003c\/span\u003e\u003cspan\u003e: Asp-Asp-Asp-Asp-Lys\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eH\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003eigh purity\u003c\/strong\u003e—\u003c\/span\u003e\u003cspan\u003eNo \u003c\/span\u003e\u003cspan\u003eother \u003c\/span\u003e\u003cspan\u003eprotease,\u003c\/span\u003e\u003cspan\u003eNo non-specific cutting\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eAnimal free\u003c\/strong\u003e—\u003cspan\u003eRecombinantly produced, free from exogenous viral contamination, and no animal-derived materials are used in the production process.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cstrong\u003eQuality Stability:\u003c\/strong\u003e Batch production ensures stable and continuous batch manufacturing; there are no differences between product batches, ensuring consistent quality.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cstrong\u003eAdequate Capacity:\u003c\/strong\u003e Yeasen possesses fermenters ranging from 5L to 1500L, meeting the varying batch requirements of different clients at different stages.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eIn the production process of fusion peptides and proteins, it is used to remove fusion proteins located at the N-terminus of proteins to remove unwanted fusion tags, ensuring the accurate N-terminal sequence of recombinant fusion proteins.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eIn the field of biopharmaceuticals, the production and preparation of GLP-1 analog peptide drugs are commonly used.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoTableGrid\" style=\"width: 99.9965%; height: 467.525px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eYeast recombinant expression\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMolecular Weight*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTheoretical value 22.7 kDa\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAppearance\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSterile liquid\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.0469px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 58.0469px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 58.0469px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mM Tris-HCl, 250 mM NaCl, 2 mM CaCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 50% Glycerol, pH 8.0\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnzyme Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e≥95%\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 231.549px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 231.549px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eActivity Definition*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 231.549px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOne unit of activity is defined as the amount of enzyme required to cut 95% of the 50 μg fusion protein with enterokinase cleavage site (Enterokinase Positive Substrate, molecular weight approximately 64.6 kDa, Cat#20391ES) under the condition of 25°C, 12-16 hours, in a buffer system (20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, pH 8.0).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eMolecular Weight: \u003c\/strong\u003e*Due to the glycosylation effect after expression in Pichia pastoris, the molecular weight of the target protein shown by SDS-PAGE is about 40 kDa.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eActivity Definition\u003cspan\u003e：\u003c\/span\u003e\u003c\/strong\u003e*The cleavage efficiency may vary depending on the substrate. Generally, smaller proteins tend to have higher cleavage efficiency. The enzyme activity defined by Yeasen is based on a large protein with a molecular weight of approximately 64.6 kDa. For oligopeptide substrates, it can generally reach a maximum of 1 unit to cleave 500 μg.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"144\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"124\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES90\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"118\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES92\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"117\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"144\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\" style=\"text-align: left;\"\u003e\u003cspan\u003eRecombinant Enterokinase, His Tag\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca name=\"OLE_LINK5\"\u003e\u003c\/a\u003e100 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"124\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e500 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e5000 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"118\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e100 KU\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"117\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e1 MU\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e1000 KU\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eStorage \u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe product can be stored at \u003c\/span\u003e\u003cspan\u003e-25\u003c\/span\u003e\u003cspan\u003e ~ \u003c\/span\u003e\u003cspan\u003e-15\u003c\/span\u003e℃ for 2 years.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eFigure\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e1. Enterokinase activity test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20395-1.jpg?v=1750754147\"\u003e\u003c\/div\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003eFigure 1. The enzyme cutting effect of Yeasen enterokinase (Cat#20395ES) is consistent with Supplier N*\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e[Note]: \u003c\/strong\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/products\/20391?_pos=1\u0026amp;_psq=20391\u0026amp;_ss=e\u0026amp;_v=1.0\"\u003e\u003cu\u003e\u003cspan class=\"16\"\u003eThe substrate (\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"16\"\u003eCat#\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"16\"\u003e20391ES)\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e is a fusion protein composed of two specific protein sequences connected by DDDDK (enterokinase recognition and cutting site), with an overall molecular weight of about 64.6 kDa, which can be specifically cut into two independent protein fragments by enterokinase, with molecular weights of about 27.9 kDa and 36.6 kDa, respectively. This product, as an enterokinase substrate, can be used for semi-quantitative or qualitative enzyme activity detection of recombinant or natural enterokinase.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e2. Enterokinase purity test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_40f713c0-8139-4916-8277-37ee0cd9248f.png?v=1750315648\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cb\u003eFigure 2. The purity of enterokinase (\u003c\/b\u003e\u003cb\u003eCat#\u003c\/b\u003e\u003cb\u003e20395ES) is greater than 95%\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e[Note]: \u003c\/strong\u003eThe theoretical value of enterokinase is 22.7 kDa. Due to the glycosylation effect after expression in Pichia pastoris, the SDS-PAGE shows that the molecular weight of the target protein is about 40 kDa. Generally, the glycosylated bands will have a slightly diffuse band running upwards (as shown in the right figure). Our company can also provide deglycosylation enzyme Endo H (Cat#20414) for customers to deglycosylate and run gel to measure purity (as shown in the left figure).\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e3. Enterokinase cutting specificity test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20395-3.jpg?v=1750762129\"\u003e\u003c\/div\u003e\n\u003cp align=\"justify\" class=\"p\" style=\"text-align: center;\"\u003e\u003cstrong\u003eFigure 3. Yeasen enterokinase (20395ES) has less non-specific cutting than Supplier N*\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\" style=\"text-align: left;\"\u003e\u003cstrong\u003e[Note]: \u003c\/strong\u003eUnder the same conditions, Yeasen enterokinase (20395ES) and supplier N's enterokinase were used for enzyme cutting tests. The results show that after purification, the non-specific cutting impurities produced by Yeasen enterokinase (20395ES) are significantly lower than those of Supplier N*.\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e4. Enterokinase repeated freeze-thaw, accelerated stability test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_4d8d90ef-43ee-4b69-85c7-f2b8c4df3c0c.png?v=1750315663\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003eFigure 4. Yeasen enterokinase (Cat#20395ES) repeated freeze-thaw, accelerated stability test shows that enzyme activity did not change significantly.\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e\u003cstrong\u003e[Note]:\u003c\/strong\u003e Yeasen enterokinase (\u003c\/span\u003e\u003cspan\u003eCat#\u003c\/span\u003e\u003cspan\u003e20395ES) randomly selected 2 batches, repeated freeze-thaw 10 times, 20 times, enzyme activity did not change significantly from the initial enzyme activity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eStorage at 25°C for 7, 16, 32 days, 37°C for 7, 14 days, enzyme activity did not change significantly from the initial enzyme activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions (Application Example)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e1.  Reaction System:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"17\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"612\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eFusion protein\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"672\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμg\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"612\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eEnterokinase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"672\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e0.1-1 U (adjustable according to actua\u003c\/span\u003e\u003cspan\u003el conditions)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"612\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eReaction \u003c\/span\u003e\u003cspan\u003eBuffer \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e20 mM Tris-HCl, 50 mM NaCl, 2 \u003c\/span\u003e\u003cspan\u003emM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCaCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003epH 8.0\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"672\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eUp to 50-500 μL(protein co\u003c\/span\u003e\u003cspan\u003encentration 0.1-1 mg\/mL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e2.  Reaction conditio\u003c\/span\u003e\u003cspan\u003ens: Mix well, react at 25°C for 16 hours.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003eNote: Recombinant enterokinase can be diluted with 25 mM Tris-HCl pH 8.0 to a solution \u003c\/span\u003e\u003cspan\u003econtaining 0.1 U per 1  μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003efor use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eThis enzyme\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ehighly\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003epotent and its activity\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003erelated to the sequence or\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003estructure\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eof the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebe\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecut.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eIt\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003erecommended to first d\u003c\/span\u003e\u003cspan\u003eo a gradient of protein input during testing, and to use the most appropriate amount.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e2. The following conditions may have a consistent effect on the activity of rE\u003c\/span\u003e\u003cspan\u003eK:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e1) High ionic strength will inhibit its activity. Under 0.25 M NaCl conditions, \u003c\/span\u003e\u003cspan\u003eit reduces the original activity of rEK by\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eabout 25%; under 2 M NaCl conditions, rEK is almost c\u003c\/span\u003e\u003cspan\u003eompletely inhibited.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e2) Reagents that inhibit its activity may include, \u0026gt;2 M Urea, \u0026gt;20 mM b\u003c\/span\u003e\u003cspan\u003e-ME, \u0026gt;0.1% SDS, \u0026gt;50 mM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eimidazole, etc.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e3) Conditions of pH\u0026lt;6 or pH\u0026gt;9 will also inhibit its a\u003c\/span\u003e\u003cspan\u003ectivity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e3. Please wear the necessary PPE, suc\u003c\/span\u003e\u003cspan\u003eh lab coat and gloves, to ensure\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eyour health and safety\u003cspan\u003e！\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e4. For research\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003euse only!\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\u003cstrong\u003eRelated reading:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/protein\/recombinant-enterokinase\"\u003e\u003cu\u003e\u003cspan class=\"15\"\u003eUnlocking the Power of Enterokinase\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294150812013,"sku":"20395ES60","price":60.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294157431149,"sku":"20395ES76","price":255.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20395-1.jpg?v=1778101064"}],"url":"https:\/\/www.ebiohippo.com\/collections\/rc-drug-development-bioanalytical-method-development.oembed?page=2","provider":"BioHippo","version":"1.0","type":"link"}