{"title":"Cancer Vectors \u0026 Viruses","description":null,"products":[{"product_id":"b7-1-cd80-orf-cdna-lentivirus-bhv19400112","title":"B7-1 (CD80) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD80, also known as B7-1, is a costimulatory cell-surface glycoprotein of the immunoglobulin superfamily expressed mainly on antigen-presenting cells such as dendritic cells, activated B cells, and macrophages. By binding the receptors CD28 and CTLA-4 on T cells, CD80 delivers a critical second signal that, together with T cell receptor engagement, governs T cell activation, proliferation, and tolerance. Engagement of CD28 promotes T cell activation, whereas binding to CTLA-4 transmits an inhibitory signal that restrains immune responses. Because the B7-CD28\/CTLA-4 axis is central to immune regulation and tumor immune evasion, CD80 is widely studied in immunology, cancer immunotherapy, and checkpoint research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe B7-1 (CD80) ORF cDNA Lentivirus enables stable overexpression of the human or mouse CD80 open reading frame (NM_005191) in mammalian cells. A CMV or EF1a promoter drives the CD80 ORF fused to a C-terminal V5 epitope tag, with a fluorescent reporter (GFP or RFP) and an antibiotic selection marker separated by a self-cleaving peptide for independent translation from a single transcript. The optimized third-generation lentiviral vector provides high gene expression and reliable genome integration, allowing rapid establishment of stable cell lines. Applications include studying CD80 costimulatory function, T cell activation, immune checkpoint biology, and tumor immunology. Particles transduce difficult-to-transfect cells, including primary and thawed cells; cloning accuracy is confirmed by sequencing and protein expression validated by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of B7-1\/CD80 (NCBI Accession: NM_005191, NM_009855) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-B7-1-V5 \/ GFP\/none \/ 5x10^6","offer_id":53251615293805,"sku":"LCV-0020-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd80-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311261507949,"sku":"LCV-0020-6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-1-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311261540717,"sku":"LCV-0020-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd80-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311261573485,"sku":"LCV-0020-5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-1-Flag \/ none\/Puromycin \/ 5x10^6","offer_id":53311261606253,"sku":"LCV-0020-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-1-Flag \/ none\/Blasticidin \/ 5x10^6","offer_id":53311261639021,"sku":"LCV-0020-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"cell-cycle-reporter-lentivirus-bhv19400048","title":"Cell Cycle Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCell cycle progression through the G1, S, G2, and M phases is tightly coordinated to ensure faithful DNA replication and division. A key mechanism of phase control is regulated protein degradation: the licensing factor CDT1 accumulates in G1 and is degraded as cells enter S phase, while Geminin accumulates during S, G2, and M and is degraded at the end of mitosis. The fluorescent ubiquitination-based cell cycle indicator (FUCCI) exploits these reciprocal oscillations by fusing fluorescent proteins to fragments of CDT1 and Geminin, allowing real-time visualization of cell cycle phase in living cells. This approach is widely used in studies of proliferation, differentiation, stem cell biology, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Cell Cycle Reporter Lentivirus delivers a FUCCI-based system for visualizing cell cycle progression in living cells without fixation. Human CDT1 (amino acids 30-120), which accumulates in G1, is fused to RFP, and human Geminin (amino acids 1-110), which accumulates in G2\/M, is fused to GFP. A CMV promoter drives RFP-CDT1, GFP-Geminin, and a drug selection marker as a single transcript, with self-cleaving T2A and P2A sequences yielding three separate proteins for balanced reporter expression.\u003c\/p\u003e\n\u003cp\u003eThis arrangement enables precise monitoring of all cell cycle phases in stable or transiently transduced cells. Supplied as VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product suits a wide range of mammalian cells, including primary, thawed, and pluripotent stem cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, GFP, hCDT1-RFP + hGemn-GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Cell cycle (phases) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615326573,"sku":"LTV-0052-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251635347821,"sku":"LTV-0052-2S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FUCCI-Puro-Fig.1.jpg?v=1776934330"},{"product_id":"p53-reporter-lentivirus-bhv19400007","title":"P53 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTP53 encodes p53, a tumor suppressor transcription factor often described as the guardian of the genome. In response to cellular stresses such as DNA damage, oncogene activation, and hypoxia, p53 is stabilized and binds specific response elements to activate genes that drive cell cycle arrest, DNA repair, senescence, and apoptosis. Through this program, p53 prevents the propagation of damaged or potentially malignant cells. TP53 is the most frequently mutated gene in human cancer, and loss of p53 function compromises these protective responses. Because of its central role, p53 transcriptional activity is widely monitored in studies of genotoxic stress, apoptosis, and tumor suppression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe P53 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of p53 activity in human or mouse cells. Tandem consensus p53 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, or Renilla luciferase), so that p53 activation produces a quantitative signal indicative of p53 and apoptosis pathway engagement in transduced cells. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study genotoxic stress responses, apoptosis, and tumor suppressor signaling, and to screen modulators of p53 activity. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Apoptosis\/p53 signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615621485,"sku":"LTV-0008-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893982061,"sku":"LTV-0008-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310894014829,"sku":"LTV-0008-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894047597,"sku":"LTV-0008-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310894080365,"sku":"LTV-0008-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894113133,"sku":"LTV-0008-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_b476d082-2641-436d-80a2-b5c8f467b270.jpg?v=1776934151"},{"product_id":"ctla4-nfat-reporter-lentivirus-bhv19400277","title":"CTLA4\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCTLA-4 (CD152) is a co-inhibitory receptor and a central regulator of immune checkpoint control. Expressed on activated T cells and constitutively on regulatory T cells, it competes with the co-stimulatory receptor CD28 for the shared B7 ligands CD80 and CD86 on antigen-presenting cells, and binds them with higher affinity. By outcompeting CD28 and removing B7 ligands from the cell surface, CTLA-4 dampens T cell activation, reduces calcium-driven NFAT signaling, and enforces peripheral tolerance. Because CTLA-4 restrains anti-tumor immunity, blocking antibodies are a foundational cancer immunotherapy, and NFAT reporters provide a quantitative readout of CTLA-4-mediated suppression and its reversal.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CTLA4\/NFAT Reporter Lentivirus is an all-in-one immunotherapy reporter system for studying CTLA-4-mediated checkpoint signaling in T cells. The construct constitutively expresses the human CTLA-4 receptor; engagement of B7 ligands suppresses T cell activity, lowering cytosolic calcium, reducing NFAT activation, and decreasing dual reporter output of secreted Gaussia luciferase and a fluorescent protein (GFP or RFP). Anti-CTLA-4 antibodies that block receptor engagement restore reporter activity.\u003c\/p\u003e\n\u003cp\u003eTransducing target cells with the high-titer lentivirus establishes a stable, pathway-specific reporter cell line. Applications include studying immune checkpoint suppression and testing checkpoint-blockade agents. Supplied as third-generation, VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, effective in primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CTLA4 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616113005,"sku":"TRV-0029-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_112d1832-d98f-436e-99de-345e734a41ce.jpg?v=1782157779"},{"product_id":"foxc2-ets-reporter-lentivirus-bhv19400227","title":"FOXC2\/ETS Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXC2 is a forkhead box transcription factor essential for developmental patterning and tissue maintenance, including lymphatic vessel formation and the development of the lungs, eyes, kidneys, urinary tract, and cardiovascular system. ETS family transcription factors, such as the endothelial regulator ETV2, control endothelial and mesodermal gene programs. FOXC2 and ETS factors can act together at composite regulatory elements, where their synergistic binding drives target genes involved in vascular and lymphatic development. This cooperative FOXC2-ETS activity is important for endothelial differentiation and has relevance to angiogenesis, lymphangiogenesis, and developmental disorders, making the combined transcriptional output an informative readout in vascular biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXC2\/ETS Reporter Lentivirus is a transcription-factor reporter system that measures the combined activity of the forkhead factor FOXC2 and the ETS family member ETV2. The construct uses tandem repeats of FOXC2 binding elements derived from the MEF2 enhancer to detect their synergistic transcriptional activity and the activation of related signaling pathways. A fluorescent or luminescent reporter, chosen from options including GFP, RFP, mCherry, BFP2, and luciferase configurations, is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying endothelial and lymphatic development and FOXC2-ETS cooperation. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXC2 \u0026amp; ETS family members synergistic activity transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616244077,"sku":"LTV-0118-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362029789549,"sku":"LTV-0118-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362029822317,"sku":"LTV-0118-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362029855085,"sku":"LTV-0118-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362029887853,"sku":"LTV-0118-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362029920621,"sku":"LTV-0118-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXC2-ETV2-TAG-PURO-FIG1-1.jpg?v=1776934147"},{"product_id":"mouse-pdl1-shrna-lentivirus-bhv19400159","title":"Mouse PDL1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePD-L1 (programmed death-ligand 1, also called B7-H1, encoded by Cd274) is a transmembrane immune checkpoint ligand expressed on tumor cells, antigen-presenting cells, and other tissues. By engaging the inhibitory receptor PD-1 on activated T cells, PD-L1 delivers a suppressive signal that limits T cell proliferation, cytokine production, and cytotoxic activity, contributing to peripheral tolerance and prevention of excessive immune responses. Tumors frequently upregulate PD-L1, often in response to interferon-gamma, to evade immune surveillance. The PD-1\/PD-L1 axis is a central target of checkpoint-blockade immunotherapy, making PD-L1 an important focus of cancer immunology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Mouse PD-L1 shRNA Lentivirus delivers validated short hairpin RNA targeting mouse Cd274 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% PD-L1 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of immune checkpoint signaling, tumor immune evasion, and checkpoint-blockade research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting Cd274 (NCBI Accession: NM_021893.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616342381,"sku":"LSV-0043-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311253840237,"sku":"LSV-0043-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311253873005,"sku":"LSV-0043-5-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311253905773,"sku":"LSV-0043-5-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311253938541,"sku":"LSV-0043-5-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311253971309,"sku":"LSV-0043-5-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311254004077,"sku":"LSV-0043-5-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311254036845,"sku":"LSV-0043-5-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311254069613,"sku":"LSV-0043-5-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311254102381,"sku":"LSV-0043-5-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0043-m-Pdl1-sh-fig1.jpg?v=1776934150"},{"product_id":"pd-l1-b7-h1-orf-cdna-lentivirus-bhv19400108","title":"PD-L1 (B7-H1) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD274 encodes PD-L1 (programmed cell death 1 ligand 1, also known as B7-H1), a transmembrane immune checkpoint ligand. By binding the inhibitory receptor PD-1 on T cells, PD-L1 dampens T cell receptor signaling, promoting peripheral tolerance and limiting immune-mediated tissue damage. Many tumors upregulate PD-L1 to suppress anti-tumor T cell responses and evade immune clearance. The PD-1\/PD-L1 axis is a central target of cancer immunotherapy, and checkpoint-blockade antibodies against this pathway are widely used clinically, making CD274 a key focus of immuno-oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PD-L1 (B7-H1) ORF cDNA Lentivirus delivers the PD-L1 (CD274) open reading frame for stable overexpression in mammalian cells. Expression of the ORF, fused to a C-terminal V5 tag, is driven by a CMV or EF1α promoter, with a GFP or RFP reporter and optional blasticidin or puromycin selection marker separated by self-cleaving peptide sequences for independent translation. The lentiviral vectors are optimized for high expression and reliable genome integration; ORF accuracy is confirmed by sequencing and protein expression validated by transient transfection.\u003c\/p\u003e\n\u003cp\u003eSupplied as high-titer, third-generation, VSV-G-pseudotyped particles, it efficiently transduces primary and thawed cells, supporting stable PD-L1-expressing cell lines for studying immune checkpoint signaling and immunotherapy.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of CD274 (NCBI Accession: NM_014143, NM_021893) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-Cd274-V5 \/ RFP\/none \/ 5x10^6","offer_id":53251616801133,"sku":"LCV-0016-6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd274-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311254724973,"sku":"LCV-0016-5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311254757741,"sku":"LCV-0016-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311254790509,"sku":"LCV-0016-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-Flag \/ none\/Puromycin \/ 5x10^6","offer_id":53311254823277,"sku":"LCV-0016-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-V5 \/ none\/Blasticidin \/ 5x10^6","offer_id":53311254856045,"sku":"LCV-0016-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDL1-Flag \/ none\/Blasticidin \/ 5x10^6","offer_id":53311254888813,"sku":"LCV-0016-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"c-myc-reporter-lentivirus-bhv19400006","title":"c-MYC Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ec-MYC is a basic helix-loop-helix leucine zipper transcription factor and a master regulator of cell growth, proliferation, metabolism, and ribosome biogenesis. By dimerizing with MAX and binding E-box elements in target promoters, c-MYC activates and represses large gene networks that coordinate the cell cycle and biosynthetic capacity. Its expression is tightly controlled in normal cells, but c-MYC is one of the most commonly deregulated oncogenes in human cancer. Aberrant c-MYC activity drives uncontrolled proliferation, metabolic reprogramming, and tumor progression, making it a central focus of oncology and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe c-MYC Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of c-MYC transcriptional activity in human or mouse cells. Reporter expression is controlled by c-MYC-responsive elements, so signal intensity reflects endogenous c-MYC activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study c-MYC-driven proliferation, metabolism, and transformation, and to screen modulators of c-MYC pathway activity. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the cMYC transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616866669,"sku":"LTV-0007-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896537965,"sku":"LTV-0007-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896570733,"sku":"LTV-0007-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896603501,"sku":"LTV-0007-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896636269,"sku":"LTV-0007-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896669037,"sku":"LTV-0007-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0007-Fig1-MYC-TAG-Puro-1.jpg?v=1776934330"},{"product_id":"h-m-hif2a-shrna-lentivirus-bhv19400124","title":"h\/m HIF2a shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that drives cellular adaptation to low oxygen. Under normoxia it is hydroxylated and degraded; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a gene set partly distinct from HIF-1α. Aberrant HIF-2α activity contributes to clear cell renal carcinoma and other tumors, where it promotes growth and vascularization, making EPAS1 an important target in cancer and hypoxia research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m HIF2a shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse EPAS1 (HIF2a) for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of hypoxia signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting EPAS1 (NCBI Accession: NM_001430) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251617259885,"sku":"LSV-0005-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311258427757,"sku":"LSV-0005-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311258460525,"sku":"LSV-0005-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311258493293,"sku":"LSV-0005-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311258526061,"sku":"LSV-0005-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311258558829,"sku":"LSV-0005-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311258591597,"sku":"LSV-0005-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311258624365,"sku":"LSV-0005-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311258657133,"sku":"LSV-0005-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311258689901,"sku":"LSV-0005-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0005-hm-HIF2a-sh-2-sh3.jpg?v=1776934211"},{"product_id":"hif1a-reporter-lentivirus-bhv19400005","title":"HIF1a Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 1 alpha (HIF-1α), encoded by HIF1A, is an oxygen-sensitive transcription factor that orchestrates the cellular response to low oxygen. Under normoxia, HIF-1α is hydroxylated and rapidly degraded; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate genes controlling glycolysis, angiogenesis, and erythropoiesis. HIF-1α drives metabolic adaptation in hypoxic tissues and tumors, where it promotes survival, vascularization, and aggressive behavior. HIF-1α is a major focus of oncology, angiogenesis, and hypoxia research, and is mechanistically distinct from the related factor HIF-2α.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF1a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-1α activity. The construct is preferentially activated by HIF-1α rather than HIF-2α, supporting discrimination between the two factors. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring hypoxia pathway activation and screening HIF-1α modulators in oncology and angiogenesis research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia\/HIF pathway (HIF-1a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617292653,"sku":"LTV-0006-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310894866797,"sku":"LTV-0006-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894899565,"sku":"LTV-0006-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310894932333,"sku":"LTV-0006-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310894965101,"sku":"LTV-0006-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894997869,"sku":"LTV-0006-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HRE-TAL-Puro-IVIS.jpg?v=1776934147"},{"product_id":"hif2a-reporter-lentivirus-bhv19400025","title":"HIF2a Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that mediates cellular adaptation to low oxygen. Under normoxia, HIF-2α is hydroxylated and targeted for degradation; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a tissue distribution and gene set partly distinct from HIF-1α. Dysregulated HIF-2α activity contributes to clear cell renal carcinoma and other tumors, making it an important target in oncology, angiogenesis, and hypoxia research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF2a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-2α (EPAS1) activity in transduced cells. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Purified by PEG precipitation and sucrose gradient centrifugation, the particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring hypoxia pathway activation, screening HIF-2α modulators, and studying angiogenesis and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia\/HIF pathway (HIF-2a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617358189,"sku":"LTV-0029-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310891426157,"sku":"LTV-0029-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310891458925,"sku":"LTV-0029-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891491693,"sku":"LTV-0029-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310891524461,"sku":"LTV-0029-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310891557229,"sku":"LTV-0029-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891589997,"sku":"LTV-0029-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_78b405ca-f48e-425b-a811-ae56b18e0bdd.jpg?v=1776934275"},{"product_id":"foxm1-reporter-lentivirus-bhv19400055","title":"FOXM1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXM1 is a member of the Forkhead box family of transcription factors, which share a conserved DNA-binding domain but differ in flanking sequences that confer target specificity. FOXM1 is a master regulator of cell cycle progression, controlling gene expression required for the G2 and M phases, including genes for mitotic entry and chromosome segregation. FOXM1 activity is tied to proliferation, DNA damage repair, and cellular senescence. It is overexpressed in many cancers, where it drives uncontrolled proliferation and tumor progression, making FOXM1 a prominent target for cancer therapy and a focus of oncology and developmental biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXM1 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of FOXM1 DNA-binding elements designed to preferentially read out FOXM1 activity over other Forkhead transcription factors. The elements drive a reporter gene downstream of a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXM1 transcriptional activity and associated cell cycle signaling. Reporter options include EGFP, GFP, mCherry, RFP, d2GFP, and firefly luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying cell cycle regulation and cancer biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the FOXM1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617423725,"sku":"LTV-0059-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893097325,"sku":"LTV-0059-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310893130093,"sku":"LTV-0059-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893162861,"sku":"LTV-0059-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310893195629,"sku":"LTV-0059-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310893228397,"sku":"LTV-0059-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0059-FOXM1-Reporter-Fig1.jpg?v=1776934148"},{"product_id":"e2f-reporter-lentivirus-bhv19400022","title":"E2F Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eE2F transcription factors are central regulators of the cell cycle, controlling the transition from G1 to S phase. By binding consensus E2F DNA elements, activator E2Fs such as E2F1 induce genes required for DNA replication and proliferation, including cyclins A and E. E2F activity is governed by the retinoblastoma protein (Rb): in G0 and early G1, hypophosphorylated Rb binds E2F and represses its target genes, while cyclin-dependent kinases phosphorylate Rb to release active E2F. This Rb-E2F axis is one of the most frequently disrupted pathways in cancer, and E2F overexpression promotes tumor development in tissues such as breast, lung, and liver, making E2F activity an important oncology research readout.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe E2F Reporter Lentivirus places a reporter gene under the control of tandem consensus E2F DNA-binding elements, providing a sensitive readout of E2F transcription factor activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring cell cycle progression and Rb-E2F pathway activity, studying proliferation in cancer models, and screening cell cycle modulators.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the E2F transcription factors transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617882477,"sku":"LTV-0026-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310900863341,"sku":"LTV-0026-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310900896109,"sku":"LTV-0026-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310900928877,"sku":"LTV-0026-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310900961645,"sku":"LTV-0026-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310900994413,"sku":"LTV-0026-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310901027181,"sku":"LTV-0026-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/E2F-Reporter-Fig.-2.jpg?v=1776934143"},{"product_id":"human-pdl1-shrna-lentivirus-bhv19400127","title":"Human PDL1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD274, also known as PD-L1 or B7-H1, is a cell-surface ligand that delivers an inhibitory signal to T cells through the PD-1 receptor. Engagement of PD-1 by PD-L1 suppresses T-cell receptor signaling, proliferation, and cytokine production, contributing to peripheral tolerance and the prevention of excessive immune responses. Many tumors and antigen-presenting cells upregulate PD-L1 in response to inflammatory signals such as interferon-gamma, exploiting this checkpoint to evade T-cell-mediated killing. The PD-1\/PD-L1 axis is a central target of cancer immunotherapy, and PD-L1 expression is widely studied as a determinant of tumor immune evasion and as a biomarker for checkpoint-blockade therapy.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PDL1 shRNA Lentivirus delivers validated short hairpin RNA targeting human CD274 (PD-L1) for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of PD-L1 using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of immune checkpoint signaling and tumor immune evasion.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting CD274 (NCBI Accession: NM_014143) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251618242925,"sku":"LSV-0010-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311259017581,"sku":"LSV-0010-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311259050349,"sku":"LSV-0010-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311259083117,"sku":"LSV-0010-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311259115885,"sku":"LSV-0010-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311259148653,"sku":"LSV-0010-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311259181421,"sku":"LSV-0010-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311259214189,"sku":"LSV-0010-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311259246957,"sku":"LSV-0010-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311259279725,"sku":"LSV-0010-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0010-h-PDL1-sh-Fig1-1.jpg?v=1776934209"},{"product_id":"erg-reporter-lentivirus-bhv19400090","title":"ERG Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eERG (ETS-related gene) is a transcription factor of the ETS family that recognizes a core GGAA DNA-binding motif with preferences for specific flanking sequences. ERG regulates embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis, and is an important regulator of endothelial and hematopoietic cell function. ERG often cooperates with the AP-1 transcription factor complex for optimal transcriptional activity. Chromosomal translocations involving ERG generate oncogenic fusion proteins, including TMPRSS2-ERG in prostate cancer, EWS-ERG in Ewing sarcoma, and FUS-ERG in acute myeloid leukemia, making ERG transcriptional activity an important readout in oncology and vascular biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ERG Reporter Lentivirus places a reporter gene under the control of consensus ERG DNA-binding elements that include an AP-1-like motif, providing a sensitive readout of ERG transcriptional activity in human and mouse cells. A broad range of reporter options is available, including fluorescent (GFP, EGFP, d2GFP, BFP2, mCherry, RFP), luminescent (firefly, Renilla, and Gaussia luciferase), and combined formats, with blasticidin, hygromycin, puromycin, or zeocin selection.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry. Applications include monitoring ERG activity in cancer and endothelial models.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the ERG transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251618406765,"sku":"LTV-0099-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251632431469,"sku":"LTV-0099-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53251632464237,"sku":"LTV-0099-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53251632497005,"sku":"LTV-0099-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53251632529773,"sku":"LTV-0099-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53251632562541,"sku":"LTV-0099-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Puromycin \/ 5x10^6","offer_id":53251632595309,"sku":"LTV-0099-15S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Blasticidin \/ 5x10^6","offer_id":53251632628077,"sku":"LTV-0099-16S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ERG-TAG-Puro-Thumbnail-1.jpg?v=1776934145"},{"product_id":"lag3-il-2-reporter-lentivirus-set-bhv19400272","title":"LAG3\/IL-2 Reporter Lentivirus Set","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eLAG3 (lymphocyte-activation gene 3) is a co-inhibitory immune checkpoint receptor expressed on activated T cells, regulatory T cells, and other lymphocytes. By binding ligands such as MHC class II and fibrinogen-like protein 1, LAG3 transmits inhibitory signals that dampen T cell activation, proliferation, and cytokine production, contributing to peripheral tolerance and limiting excessive immune responses. In tumors, sustained LAG3 expression marks exhausted T cells and supports immune evasion. LAG3 is a validated immunotherapy target, and antibodies that block LAG3 are being developed to restore antitumor T cell function, often in combination with other checkpoint inhibitors.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe LAG3\/IL-2 Reporter Lentivirus Set is a two-component immunotherapy reporter system for studying the LAG3 immune checkpoint. One lentivirus constitutively expresses the human LAG3 receptor; the second carries an IL-2-responsive dual reporter combining Gaussia luciferase with a fluorescent protein (eGFP or mCherry). Ligand engagement of LAG3 suppresses IL-2-driven reporter expression, while checkpoint inhibitors that block LAG3 restore the signal, providing a quantitative readout of checkpoint blockade. Sequential transduction with puromycin and blasticidin selection generates a dual-stable effector cell line. The system is used to evaluate LAG3-targeting antibodies and to monitor T cell activation in immuno-oncology research. Supplied as high-titer, VSV-G pseudotyped lentiviral particles suitable for difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human LAG3 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"Gaussia Luciferase + eGFP \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53251618439533,"sku":"TRV-0024-SET1","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Immunotherapy-Reporter-Set-Construct-Diagrams-1_11e09355-cad1-41a4-a956-d051578f27a2.jpg?v=1776934219"},{"product_id":"h-m-hif1a-shrna-lentivirus-bhv19400123","title":"h\/m HIF1a shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHIF1A encodes hypoxia-inducible factor 1-alpha, the oxygen-sensitive subunit of the HIF-1 transcription factor that coordinates the cellular response to low oxygen. Under normal oxygen levels, prolyl hydroxylases modify HIF-1α, marking it for von Hippel-Lindau-mediated degradation. Under hypoxia, this degradation is suppressed, allowing HIF-1α to dimerize with HIF-1β and activate genes that promote angiogenesis, glycolysis, erythropoiesis, and metabolic adaptation. HIF-1 signaling is essential for development and ischemic tissue responses, and it is frequently hyperactivated in solid tumors, where it drives angiogenesis, metabolic reprogramming, and resistance to therapy. HIF-1α is therefore a key focus of cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m HIF1a shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse HIF1A for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of HIF1A using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of hypoxia signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting HIF1A (NCBI Accession: NM_001530) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"None \/ Blasticidin \/ 2x10^6","offer_id":53251618505069,"sku":"LSV-0004-T2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 2x10^6","offer_id":53311255085421,"sku":"LSV-0004-T1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311255118189,"sku":"LSV-0004-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311255150957,"sku":"LSV-0004-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255183725,"sku":"LSV-0004-4-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255216493,"sku":"LSV-0004-4-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311255249261,"sku":"LSV-0004-4-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311255282029,"sku":"LSV-0004-4-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255314797,"sku":"LSV-0004-4-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255347565,"sku":"LSV-0004-4-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255380333,"sku":"LSV-0004-4-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255413101,"sku":"LSV-0004-4-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0004-hm-HIF1a-sh-Fig1.jpg?v=1776934150"},{"product_id":"kras-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400100","title":"KRAS (Wild Type and Mutant) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eKRAS is a proto-oncogene encoding a small GTPase that acts as a molecular switch in growth-factor signaling. Cycling between an active GTP-bound and inactive GDP-bound state, KRAS relays signals from receptor tyrosine kinases to downstream effectors, principally the RAF-MEK-ERK and PI3K-AKT pathways, controlling cell proliferation, differentiation, and survival. Oncogenic point mutations, most commonly at codons 12, 13, and 61, lock KRAS in the active GTP-bound state, producing constitutive signaling. Such mutations are among the most frequent in human cancer, occurring at high rates in pancreatic, colorectal, and lung adenocarcinomas, and drive tumor initiation and progression. KRAS is therefore a central focus of cancer biology and targeted-therapy research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe KRAS (Wild Type and Mutant) ORF cDNA Lentivirus delivers the human KRAS open reading frame (transcript variant a) for stable overexpression in mammalian cells, available as wild type or as common oncogenic mutants. An EF1a promoter drives expression of KRAS fused to a C-terminal V5 tag, linked via self-cleaving peptides to a fluorescent reporter (GFP or RFP) or firefly luciferase and a drug-selection marker (puromycin or blasticidin); in some constructs a PGK promoter drives the reporter and selection cassette separately. The lentiviral vectors are optimized for high expression and reliable genome integration, and the particles efficiently transduce difficult-to-transfect cells, including primary and thawed cultures, supporting stable cell line generation for studies of RAS signaling and oncogenic transformation. ORF integrity is verified by sequencing and expression confirmed by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of KRAS (NCBI Accession: NM_033360) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (EF1a). Reporter and selection marker components (Firefly Luciferase, GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"EF1a-KRAS-G12C-Flag \/ none\/Puromycin \/ 3x10^6","offer_id":53251618537837,"sku":"LCV-0008-11-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-V5 \/ GFP\/none \/ 3x10^6","offer_id":53311256854893,"sku":"LCV-0008-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-G12V-Flag \/ none\/Blasticidin \/ 3x10^6","offer_id":53311256887661,"sku":"LCV-0008-10-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-G12D-V5 \/ Firefly Luciferase\/none \/ 2x10^6","offer_id":53311256920429,"sku":"LCV-0008-09-5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-G12V-Flag \/ none\/Puromycin \/ 3x10^6","offer_id":53311256953197,"sku":"LCV-0008-10-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-Flag \/ none\/Puromycin \/ 3x10^6","offer_id":53311256985965,"sku":"LCV-0008-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-G12D-Flag \/ none\/Blasticidin \/ 3x10^6","offer_id":53311257018733,"sku":"LCV-0008-09-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-G12D-Flag \/ none\/Puromycin \/ 3x10^6","offer_id":53311257051501,"sku":"LCV-0008-09-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-G12C-Flag \/ none\/Blasticidin \/ 3x10^6","offer_id":53311257084269,"sku":"LCV-0008-11-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-V5 \/ RFP\/none \/ 3x10^6","offer_id":53311257117037,"sku":"LCV-0008-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1a-KRAS-Flag \/ none\/Blasticidin \/ 3x10^6","offer_id":53311257149805,"sku":"LCV-0008-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"hif1a-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400096","title":"HIF1A Wild Type \u0026 Mutant ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHIF1A encodes hypoxia-inducible factor 1-alpha, the oxygen-sensitive subunit of the HIF-1 transcription factor that orchestrates the cellular response to low oxygen. Under normoxia, prolyl hydroxylases modify conserved proline residues in HIF-1α, marking it for von Hippel-Lindau-mediated ubiquitination and proteasomal degradation. Under hypoxia, this degradation is blocked, allowing HIF-1α to dimerize with HIF-1β and activate genes governing angiogenesis, glycolysis, erythropoiesis, and metabolic adaptation. HIF-1 signaling is central to development, ischemic disease, and tumor biology, where it drives angiogenesis and metabolic reprogramming. Mutating the regulatory proline and asparagine residues (P402A\/P577A\/N813A) yields a stabilized protein that remains active under normoxic conditions.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF1A Wild Type \u0026amp; Mutant ORF cDNA Lentivirus delivers the human or mouse HIF1A open reading frame for stable overexpression in mammalian cells, available in wild-type form and as a normoxia-stable mutant (P402A\/P577A\/N813A) resistant to oxygen-mediated degradation. The ORF is fused to a C-terminal V5 tag and linked via self-cleaving peptides to a fluorescent reporter (GFP or RFP) or luciferase and a drug-selection marker (puromycin or blasticidin); expression is driven by a CMV or EF1a promoter, with PGK driving reporter and selection cassettes in some constructs. The particles efficiently transduce difficult-to-transfect cells, including primary and thawed cultures, supporting stable cell line generation for studies of hypoxia signaling, angiogenesis, and tumor metabolism. ORF integrity is verified by sequencing and expression confirmed by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of HIF1A (NCBI Accession: NM_001530, NM_010431) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-HIF1A-V5 \/ GFP\/none \/ 2x10^6","offer_id":53251619094893,"sku":"LCV-0001-H8","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ RFP\/Blasticidin \/ 2x10^6","offer_id":53311259672941,"sku":"LCV-0001-H6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ GFP\/none \/ 2x10^6","offer_id":53311259705709,"sku":"LCV-0001-H5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ none\/Blasticidin \/ 2x10^6","offer_id":53311259738477,"sku":"LCV-0001-H7","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a-V5 \/ GFP\/none \/ 2x10^6","offer_id":53311259771245,"sku":"LCV-0001-M5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ GFP\/none \/ 2x10^6","offer_id":53311259804013,"sku":"LCV-0001-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ RFP\/none \/ 2x10^6","offer_id":53311259836781,"sku":"LCV-0001-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ none\/Puromycin \/ 2x10^6","offer_id":53311259869549,"sku":"LCV-0001-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ none\/Blasticidin \/ 2x10^6","offer_id":53311259902317,"sku":"LCV-0001-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"sre-reporter-lentivirus-bhv19400024","title":"SRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe serum response element (SRE) is a promoter motif that mediates transcriptional responses to growth factor and mitogen signaling, classically studied in the c-Fos promoter. The SRE is bound by serum response factor (SRF) together with ternary complex factors such as ELK-1. Growth factor stimulation activates the MAPK\/ERK cascade, leading to ERK1\/2 (MAPK3\/MAPK1)-mediated phosphorylation of ELK-1, which together with SRF drives rapid induction of immediate-early genes. This pathway controls cell proliferation, differentiation, and survival, and its dysregulation is implicated in cancer and other proliferative disorders, making SRE-driven reporters valuable for monitoring MAPK\/ERK and growth factor signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe SRE Reporter Lentivirus places a reporter gene under the control of tandem c-Fos serum response elements, providing a sensitive readout of MAPK\/ERK and growth factor signaling through serum response factor and its cofactors, including ELK-1. Reporter options include fluorescent (GFP, RFP), luminescent (firefly and Renilla luciferase), and combined GFP\/Luc and RFP\/Luc formats, with blasticidin or puromycin selection for stable reporter cell line establishment.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring MAPK\/ERK pathway activation, studying mitogen and growth factor responses, and screening modulators of this signaling axis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, GFP\/Luc, Luc, Renilla Luc, RFP, RFP\/Luc reporter gene under the control of tandem consensus response elements specific for the MAPK\/ERK pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251619455341,"sku":"LTV-0028-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898962797,"sku":"LTV-0028-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898995565,"sku":"LTV-0028-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899028333,"sku":"LTV-0028-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310899061101,"sku":"LTV-0028-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310899093869,"sku":"LTV-0028-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899126637,"sku":"LTV-0028-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0028-SRE-TAG-Puro-Fig.-2-FBS-treatment.jpg?v=1776934451"},{"product_id":"h-m-egfr-shrna-lentivirus-bhv19400167","title":"h\/m EGFR shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eEGFR encodes the epidermal growth factor receptor, a receptor tyrosine kinase that binds ligands such as EGF and TGF-α. Ligand binding triggers receptor dimerization and autophosphorylation, activating downstream RAS\/MAPK, PI3K\/AKT, and STAT signaling cascades that control cell proliferation, survival, migration, and differentiation. EGFR is a well-established oncogene: amplification, overexpression, and activating mutations drive many epithelial cancers, including lung, colorectal, and head and neck tumors. EGFR is one of the most prominent targets of cancer therapy, with both antibody and small-molecule inhibitors in clinical use, making it a central focus of cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m EGFR shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse EGFR for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of EGFR signaling and tumor cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting EGFR (NCBI Accession: NM_005228.5) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251619520877,"sku":"LSV-0051-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311267176813,"sku":"LSV-0051-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311267209581,"sku":"LSV-0051-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311267242349,"sku":"LSV-0051-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311267275117,"sku":"LSV-0051-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311267307885,"sku":"LSV-0051-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311267340653,"sku":"LSV-0051-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311267373421,"sku":"LSV-0051-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311267406189,"sku":"LSV-0051-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311267438957,"sku":"LSV-0051-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0051-hm-EGFR-9p-1.jpg?v=1776934150"},{"product_id":"n-myc-reporter-lentivirus-bhv19400228","title":"N-MYC Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eN-MYC (MYCN) is a basic helix-loop-helix-leucine-zipper transcription factor of the MYC family. It heterodimerizes with its partner protein MAX and binds E-box DNA sequences to activate transcription of genes that drive cell growth, proliferation, ribosome biogenesis, and metabolism. During development, N-MYC is expressed in a lineage-specific manner and is essential for central nervous system formation and the proliferation of neural progenitor cells. Amplification or deregulated expression of MYCN is a well-established oncogenic event in aggressive childhood tumors, most notably neuroblastoma and medulloblastoma. Reporter systems with E-box response elements provide a quantitative readout of N-MYC transcriptional output for studies of development and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe N-MYC Reporter Lentivirus is a transcription-factor reporter system that detects N-MYC-driven transcriptional activity in mammalian cells. The construct uses tandem consensus E-box motifs with optimized flanking sequences to read out N-MYC activity selectively, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines and flexible detection by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and are suited to difficult-to-transfect cells, supporting research on N-MYC in CNS development, lineage specification, and oncogenesis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the N-MYC transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"N-MYC-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251622699373,"sku":"LTV-0120-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087100781,"sku":"LTV-0120-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087133549,"sku":"LTV-0120-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362087166317,"sku":"LTV-0120-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087199085,"sku":"LTV-0120-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087231853,"sku":"LTV-0120-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362087264621,"sku":"LTV-0120-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362087297389,"sku":"LTV-0120-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362087330157,"sku":"LTV-0120-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362087362925,"sku":"LTV-0120-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362087395693,"sku":"LTV-0120-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/N-MYC-Reporter-Figure.jpg?v=1776934149"},{"product_id":"h-m-nrp1-shrna-lentivirus-bhv19400169","title":"h\/m NRP1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuropilin 1 (NRP1) is a single-pass transmembrane glycoprotein that functions as a co-receptor for multiple ligand families, including class 3 semaphorins and members of the VEGF family. By partnering with plexins and VEGF receptors, NRP1 modulates axon guidance, vascular development, and angiogenesis. NRP1 is also expressed on immune cells, where it influences regulatory T cell function and dendritic cell activity. In cancer, NRP1 promotes tumor angiogenesis, cell migration, and invasion, and contributes to an immunosuppressive microenvironment. These roles make NRP1 a focus of research in vascular biology, neurodevelopment, immunology, and oncology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m NRP1 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse NRP1 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% NRP1 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option supplies a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of angiogenesis, axon guidance, immune regulation, and tumor progression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting NRP1 (NCBI Accession: NM_003873.7) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251622961517,"sku":"LSV-0053-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311268061549,"sku":"LSV-0053-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311268094317,"sku":"LSV-0053-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311268127085,"sku":"LSV-0053-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311268159853,"sku":"LSV-0053-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311268192621,"sku":"LSV-0053-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311268225389,"sku":"LSV-0053-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311268258157,"sku":"LSV-0053-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311268290925,"sku":"LSV-0053-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311268323693,"sku":"LSV-0053-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0053-hm-NRP1-sh-FIG1.jpg?v=1776934279"},{"product_id":"pdgfd-orf-cdna-lentivirus-bhv19400098","title":"PDGFD ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGF-D (platelet-derived growth factor D) is a member of the PDGF family of growth factors. It is secreted as a latent protein that requires proteolytic removal of its CUB domain to release the active growth-factor domain, which then signals primarily through the PDGF receptor beta. PDGF-D signaling stimulates the proliferation, migration, and survival of mesenchymal cells such as fibroblasts and smooth muscle cells, and contributes to angiogenesis, wound healing, and tissue remodeling. Aberrant PDGF-D activity has been linked to fibrosis, atherosclerosis, and tumor progression, where it can promote a supportive microenvironment, making PDGF-D a target of interest in cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PDGFD ORF cDNA Lentivirus delivers the open reading frame of human PDGF-D (transcript variant 1) for stable overexpression in mammalian cells. A CMV promoter drives expression of PDGF-D fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and a drug selection marker (puromycin or blasticidin) separated from the cDNA by self-cleaving peptides for independent translation. In some configurations a PGK promoter drives the reporter and selection marker.\u003c\/p\u003e\n\u003cp\u003eThe vectors are optimized for high expression and reliable genome integration, enabling long-term stable cell lines. ORF cloning accuracy is confirmed by sequencing and protein expression validated by transient transfection. Particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of PDGFD (NCBI Accession: NM_025208) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Puromycin, Blasticidin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-PDGFD-V5\/GFP \/ RFP \/ 5x10^6","offer_id":53251623059821,"sku":"LCV-0003-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-V5\/RFP \/ RFP \/ 5x10^6","offer_id":53311264424301,"sku":"LCV-0003-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-Flag\/Puro \/ N\/A \/ 5x10^6","offer_id":53311264457069,"sku":"LCV-0003-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-Flag\/BSD \/ N\/A \/ 5x10^6","offer_id":53311264489837,"sku":"LCV-0003-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-pdgfb-shrna-lentivirus-bhv19400158","title":"Human PDGFB shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGFB encodes the platelet-derived growth factor subunit B, a polypeptide that assembles into PDGF-BB homodimers and PDGF-AB heterodimers. Secreted PDGF ligands signal through PDGF receptor tyrosine kinases to stimulate the proliferation, migration, and survival of mesenchymal cells such as fibroblasts, smooth muscle cells, and pericytes. PDGF-B signaling is essential for blood vessel maturation and pericyte recruitment, wound healing, and connective tissue formation. Dysregulated PDGFB expression contributes to fibrosis, vascular disease, and tumor growth, where it promotes angiogenesis and an activated tumor stroma, making PDGFB an important target in cancer and cardiovascular research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PDGFB shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human platelet-derived growth factor subunit B (PDGFB). The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of PDGF-B signaling in cell proliferation, angiogenesis, fibrosis, and tumor stroma biology. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting PDGFB (NCBI Accession: NM_002608.4) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623256429,"sku":"LSV-0042-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311258722669,"sku":"LSV-0042-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311258755437,"sku":"LSV-0042-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311258788205,"sku":"LSV-0042-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311258820973,"sku":"LSV-0042-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311258853741,"sku":"LSV-0042-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311258886509,"sku":"LSV-0042-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311258919277,"sku":"LSV-0042-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311258952045,"sku":"LSV-0042-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311258984813,"sku":"LSV-0042-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0042-h-PDGFB-sh-fig1.jpg?v=1776934279"},{"product_id":"h-m-nrp2-shrna-lentivirus-bhv19400161","title":"h\/m NRP2 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuropilin 2 (NRP2) is a single-pass transmembrane glycoprotein that functions as a co-receptor for class 3 semaphorins and for members of the vascular endothelial growth factor (VEGF) family. By modulating semaphorin and VEGF signaling, NRP2 regulates axon guidance, lymphatic and vascular development, and cell migration. NRP2 is also expressed on immune cells and tumor cells, where it influences cell adhesion, motility, and survival. Aberrant NRP2 expression contributes to tumor progression, lymphangiogenesis, and metastasis in several cancers, making it an actively investigated target in cancer biology, angiogenesis research, and neurodevelopmental studies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m NRP2 shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human and mouse Neuropilin 2. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of NRP2 in tumor progression, lymphangiogenesis, cell migration, and semaphorin\/VEGF signaling. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting NRP2 (NCBI Accession: NM_201266.2) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623354733,"sku":"LSV-0045-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311265571181,"sku":"LSV-0045-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311265603949,"sku":"LSV-0045-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311265636717,"sku":"LSV-0045-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311265669485,"sku":"LSV-0045-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311265702253,"sku":"LSV-0045-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311265735021,"sku":"LSV-0045-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311265767789,"sku":"LSV-0045-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311265800557,"sku":"LSV-0045-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311265833325,"sku":"LSV-0045-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0045-hm-NRP2-sh-fig1-11-22.jpg?v=1776934279"},{"product_id":"human-pdgfd-shrna-lentivirus-bhv19400136","title":"Human PDGFD shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGFD encodes platelet-derived growth factor D, a member of the PDGF family of growth factors. PDGF-D is secreted as a latent dimer that is proteolytically activated and signals primarily through the PDGF receptor beta to drive proliferation, migration, and survival of mesenchymal cells, including fibroblasts and vascular smooth muscle cells. PDGF-D signaling contributes to angiogenesis, tissue remodeling, and fibrosis. Elevated PDGF-D expression promotes tumor growth, epithelial-mesenchymal transition, and metastasis in several cancers, making it a target of interest in cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PDGFD shRNA Lentivirus delivers a validated short hairpin RNA targeting human PDGFD for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of PDGF-D signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting PDGFD (NCBI Accession: NM_025208) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623879021,"sku":"LSV-0021-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311256559981,"sku":"LSV-0021-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311256592749,"sku":"LSV-0021-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311256625517,"sku":"LSV-0021-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311256658285,"sku":"LSV-0021-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311256691053,"sku":"LSV-0021-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311256723821,"sku":"LSV-0021-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311256756589,"sku":"LSV-0021-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311256789357,"sku":"LSV-0021-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311256822125,"sku":"LSV-0021-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0021-h-PDGFD-sh-fig1.jpg?v=1776934151"},{"product_id":"stat3-reporter-lentivirus-bhv19400003","title":"STAT3 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSTAT3 (signal transducer and activator of transcription 3) is a latent cytoplasmic transcription factor and a central effector of the JAK\/STAT signaling pathway. In response to cytokines such as IL-6 and growth factors, receptor-associated Janus kinases phosphorylate STAT3, which then dimerizes, translocates to the nucleus, and binds specific response elements to drive transcription. STAT3 target genes regulate cell proliferation, survival, angiogenesis, and immune responses. Persistent STAT3 activation is a common feature of many cancers and inflammatory conditions, where it promotes tumor growth and immune evasion. Because of this, STAT3 is an extensively studied node in cytokine signaling and oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe STAT3 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of STAT3 activity in human or mouse cells. Tandem consensus STAT3 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, Renilla luciferase, or SEAP), enabling quantitative monitoring of JAK\/STAT pathway activation in transduced cells. Constitutively expressed fluorescent reporters and antibiotic selection markers (puromycin or blasticidin) support tracking of transduced cells and establishment of stable reporter cell lines. The system is used to study cytokine signaling, screen pathway modulators, and characterize STAT3 activity in cancer and inflammation research. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the STAT3 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed)) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251624468845,"sku":"LTV-0003-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310892867949,"sku":"LTV-0003-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892900717,"sku":"LTV-0003-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310892933485,"sku":"LTV-0003-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310892966253,"sku":"LTV-0003-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Puromycin \/ 2x10^6","offer_id":53310892999021,"sku":"LTV-0003-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Blasticidin \/ 2x10^6","offer_id":53310893031789,"sku":"LTV-0003-8S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893064557,"sku":"LTV-0003-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0003-STAT3-Reporter-A549-stable-cell-line.jpg?v=1776934331"},{"product_id":"rre-reporter-lentivirus-bhv19400242","title":"RRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RAS-ERK pathway is a central signaling cascade in which growth factor activation of RAS GTPases drives the RAF-MEK-ERK kinase module, ultimately altering gene transcription that governs cell proliferation, differentiation, and survival. Activated ERK regulates transcription factors that bind the Ras-responsive element (RRE), including ETS family members such as ERG, ETV1, ETV4, and ETV5, as well as RREB-1. These factors mediate the gene expression changes associated with oncogenic transformation. Aberrant RAS-ERK signaling, whether through RAS mutation or ETS gene rearrangement, is a frequent driver of human cancers, making RRE-dependent transcription an informative readout of pathway activity and a target for inhibitor discovery.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RRE Reporter Lentivirus is a transcription-factor reporter system designed to detect transcription downstream of RAS-ERK signaling. The construct contains tandem repeats of the Ras-responsive element coupled to a minimal promoter, driving a fluorescent or luminescent reporter chosen from options including GFP, RFP, mCherry, BFP2, firefly luciferase, Gaussia luciferase, and dual configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation, with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe system is suited to monitoring RAS-ERK pathway activation, screening pathway inhibitors, and studying oncogenic signaling driven by ETS family transcription factors and RREB-1. Particles are purified by PEG precipitation and sucrose gradient centrifugation for effective transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the RAS-ERK Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"RRE-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251624993133,"sku":"LTV-0135-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087428461,"sku":"LTV-0135-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087461229,"sku":"LTV-0135-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362087493997,"sku":"LTV-0135-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087526765,"sku":"LTV-0135-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087559533,"sku":"LTV-0135-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362087592301,"sku":"LTV-0135-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362087625069,"sku":"LTV-0135-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362087657837,"sku":"LTV-0135-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362087690605,"sku":"LTV-0135-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RRE-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362087723373,"sku":"LTV-0135-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/rre.jpg?v=1776934141"},{"product_id":"h-m-p53-shrna-lentivirus-bhv19400149","title":"h\/m P53 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ep53, encoded by TP53, is a transcription factor and one of the most important tumor suppressors in the cell. In response to DNA damage, oncogene activation, and other stresses, p53 is stabilized and activates programs of cell cycle arrest, DNA repair, senescence, and apoptosis to prevent the propagation of damaged cells. p53 also regulates metabolism, autophagy, and ferroptosis. TP53 is the most frequently mutated gene in human cancer, and loss of p53 function permits genomic instability and uncontrolled proliferation. Because of its central role in guarding genome integrity, p53 is a major focus of cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m P53 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse TP53 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% p53 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of tumor suppression, the DNA damage response, cell cycle control, and apoptosis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting TP53 (NCBI Accession: NM_000546.6) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"None \/ Blasticidin \/ 2x10^6","offer_id":53251625124205,"sku":"LSV-0034-T2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 2x10^6","offer_id":53311259312493,"sku":"LSV-0034-T1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311259345261,"sku":"LSV-0034-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311259378029,"sku":"LSV-0034-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311259410797,"sku":"LSV-0034-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311259443565,"sku":"LSV-0034-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311259476333,"sku":"LSV-0034-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311259509101,"sku":"LSV-0034-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311259541869,"sku":"LSV-0034-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311259574637,"sku":"LSV-0034-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311259607405,"sku":"LSV-0034-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311259640173,"sku":"LSV-0034-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0034-h-P53-sh-fig1.jpg?v=1776934281"},{"product_id":"tigit-nfat-reporter-lentivirus-bhv19400280","title":"TIGIT\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTIGIT (T cell immunoreceptor with Ig and ITIM domains) is a co-inhibitory receptor expressed on activated T cells, regulatory T cells, and natural killer cells. By competing with the co-stimulatory receptor CD226 for the ligands CD155 and CD112, TIGIT dampens T cell and NK cell activation and contributes to peripheral tolerance and tumor immune evasion. TIGIT engagement suppresses NFAT-dependent transcription downstream of the T cell receptor, so NFAT-driven reporter systems provide a quantitative readout of TIGIT signaling. TIGIT is an actively pursued immune checkpoint, and such reporters are widely used to characterize checkpoint-blockade antibodies and other immunotherapy candidates.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe TIGIT\/NFAT Reporter Lentivirus is a two-vial immunotherapy reporter system for studying TIGIT signaling. Vial 1 constitutively expresses the human TIGIT receptor with an antibiotic selection marker, while Vial 2 carries tandem NFAT response elements driving a dual reporter combining secreted Gaussia luciferase (GLuc) and a fluorescent protein (GFP or RFP). Sequential transduction and selection generate a dual-stable effector cell line that responds quantitatively to receptor engagement. Secreted GLuc accumulates in conditioned media for kinetic, lysis-free sampling, while the fluorescent reporter supports microscopy and flow cytometry. Applications include studying immune suppression mechanisms, evaluating checkpoint inhibitor therapies, and characterizing T cell activation. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human TIGIT under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251625189741,"sku":"TRV-0032-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_f8e17f8b-5b2a-4a66-bb7e-22a1456f4544.jpg?v=1782157779"},{"product_id":"h-m-wnt16-shrna-lentivirus-bhv19400208","title":"h\/m WNT16 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eWNT16 (Wnt Family Member 16) is a secreted glycoprotein of the Wnt family of signaling ligands that act through Frizzled receptors to regulate both canonical (β-catenin-dependent) and non-canonical Wnt pathways. WNT16 is a notable regulator of bone mass and skeletal homeostasis, influencing cortical bone thickness, osteoblast and osteoclast activity, and fracture susceptibility, and has been identified in genome-wide studies of bone mineral density. Wnt signaling more broadly controls cell proliferation, differentiation, and tissue patterning, and its dysregulation contributes to skeletal disorders and cancer, making WNT16 a relevant target for bone biology and oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m WNT16 shRNA Lentivirus provides high-titer lentiviral particles for stable knockdown of human and mouse WNT16. The shRNA is delivered from a third-generation, self-inactivating backbone with expression from a U6 Pol III promoter, alongside a constitutively expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and an antibiotic selection marker. VSV-G pseudotyping enables broad tropism, including primary, suspension, and cryopreserved cells. The shRNA is validated to achieve at least 70% knockdown using a fluorescence-based assay. The set comprises lentivirus produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. It is used to study WNT16 loss of function in Wnt signaling, bone biology, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting WNT16 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251625484653,"sku":"LSV-0063-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53362029330797,"sku":"LSV-0063-1S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hm-WNT16-shRNA-Figure.jpg?v=1776934211"},{"product_id":"tcf-lef-reporter-lentivirus-bhv19400010","title":"TCF\/LEF Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTCF\/LEF transcription factors, including TCF7, LEF1, and TCF7L2, are the nuclear effectors of the canonical Wnt signaling pathway. In the absence of Wnt ligand, beta-catenin is continuously degraded and TCF\/LEF factors repress target genes. When Wnt signaling is active, beta-catenin is stabilized, accumulates in the nucleus, and partners with TCF\/LEF proteins at Wnt response elements to activate transcription. This pathway governs cell proliferation, fate determination, stem cell maintenance, and tissue patterning during development and homeostasis. Aberrant activation of Wnt\/beta-catenin signaling, often through mutations affecting beta-catenin or its regulators, is a major driver of colorectal and other cancers, making this pathway a central focus of developmental biology and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe TCF\/LEF Reporter Lentivirus is a TOPFlash-style transcription factor reporter system that provides a fluorescent or luminescent readout of Wnt\/beta-catenin pathway activation in mammalian cells. The construct places a reporter gene (d2GFP, GFP, RFP, firefly luciferase, or Renilla luciferase) under the control of tandem Wnt response elements coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise. Reporter activity is driven primarily by LEF1, with contributions from TCF1 and context-dependent TCF4. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying Wnt\/beta-catenin signaling in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Wnt\/b-catenin pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625386349,"sku":"LTV-0011-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310902698349,"sku":"LTV-0011-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310902731117,"sku":"LTV-0011-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310902763885,"sku":"LTV-0011-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310902796653,"sku":"LTV-0011-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310902829421,"sku":"LTV-0011-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Selection-Puromycin \/ Puromycin \/ 5x10^6","offer_id":53310902862189,"sku":"LTV-0011-1D2","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Selection-Puromycin \/ Puromycin \/ 2x10^6","offer_id":53310902894957,"sku":"LTV-0011-1SMT","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TOPFlash-HEK-reporter-2.jpg?v=1776934150"},{"product_id":"fluorescence-and-luciferase-dual-reporter-lentivirus-bhv19400181","title":"Fluorescence and Luciferase Dual Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTracking the location, abundance, and behavior of defined cell populations is fundamental to cancer and immunology research. Bioluminescence imaging with luciferase enables sensitive, longitudinal monitoring of cell number in living animals, while fluorescent proteins allow single-cell identification by microscopy and flow cytometry. Combining both modalities in the same cell provides complementary readouts: luciferase for whole-body imaging of tumor growth and metastasis, and fluorescence for ex vivo histology, FACS-based isolation, and analysis of tumor-infiltrating immune cells. Stable, division-resistant labeling is essential for accurate longitudinal preclinical studies of tumor biology and the tumor microenvironment.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Fluorescence and Luciferase Dual Reporter Lentivirus constitutively co-expresses a luminescent reporter (firefly or secreted Gaussia luciferase) and a fluorescent protein (GFP or RFP) from a single transcript, using self-cleaving peptides for independent, equimolar protein production. Expression is driven by a CMV, PGK, or EF1α promoter, and a selection marker (puromycin or blasticidin) supports stable cell line generation. Stable integration ensures persistent dual-reporter expression, enabling longitudinal bioluminescence imaging of tumor growth and metastasis in vivo and parallel fluorescence-based identification, gating, or FACS sorting of tumor cells for flow cytometry, scRNA-seq, and proteomics. Multiple vector layouts optimize for stable line establishment or primary-cell labeling. Supplied as ultra-purified, high-titer lentiviral particles suitable for in vitro and in vivo use, including transduction of primary and cryopreserved cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentivirus constitutively co-expresses a luciferase reporter and a fluorescent protein (GFP, RFP, Luc) from a strong CMV or EF1α promoter, using a self-cleaving P2A peptide to ensure equimolar, independent production of both reporters. A selection marker (Blasticidin, Puromycin) is co-encoded for stable cell line generation.\u003c\/p\u003e\n\u003cp\u003eThe stable lentiviral integration ensures persistent, division-stable reporter expression in the target cell line, enabling longitudinal bioluminescence imaging (IVIS) of tumor burden in mouse models and parallel fluorescence-based readouts for ex vivo analysis. The dual-modality design provides built-in redundancy — luciferase for high-sensitivity whole-body imaging and fluorescence for histology, flow cytometry, and FACS — within a single stably engineered cell line.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-Luc-GFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53251625812333,"sku":"LFV-0001-01","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-RFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53362028544365,"sku":"LFV-0001-02","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-GFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028577133,"sku":"LFV-0001-03","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-RFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028609901,"sku":"LFV-0001-04","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GFP-Puro-Luc \/ Puromycin \/ 3.5x10^6","offer_id":53362028642669,"sku":"LFV-0001-05","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-RFP-Puro-Luc \/ Puromycin \/ 3.5x10^6","offer_id":53362028675437,"sku":"LFV-0001-06","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GFP-BSD-Luc \/ Blasticidin \/ 3.5x10^6","offer_id":53362028708205,"sku":"LFV-0001-07","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-RFP-BSD-Luc \/ Blasticidin \/ 3.5x10^6","offer_id":53362028740973,"sku":"LFV-0001-08","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-GFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53362028773741,"sku":"LFV-0001-09","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-RFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028806509,"sku":"LFV-0001-10","price":595.0,"currency_code":"USD","in_stock":true},{"title":"PGK-GFP-P2A-Luc \/ N\/A \/ 5x10^6","offer_id":53362028839277,"sku":"LFV-0001-11","price":595.0,"currency_code":"USD","in_stock":true},{"title":"PGK-RFP-P2A-Luc \/ N\/A \/ 5x10^6","offer_id":53362028872045,"sku":"LFV-0001-12","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-P2A-GFP \/ N\/A \/ 5x10^6","offer_id":53362028904813,"sku":"LFV-0001-13","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-P2A-RFP \/ N\/A \/ 5x10^6","offer_id":53362028937581,"sku":"LFV-0001-14","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-RFP-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362028970349,"sku":"LFV-0001-16","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-GFP-Puro \/ Puromycin \/ 5x10^6","offer_id":53362029003117,"sku":"LFV-0001-15","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-RFP-Puro \/ Puromycin \/ 5x10^6","offer_id":53362029035885,"sku":"LFV-0001-18","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-GFP-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362029068653,"sku":"LFV-0001-17","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-rFLuc-RFP-BSD \/ Blasticidin \/ 2x10^6","offer_id":53362029101421,"sku":"LFV-0001-19","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-ZsG-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362029134189,"sku":"LFV-0001-20","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LucFluo-Fig1-JPG-3.jpg?v=1776934330"},{"product_id":"crma-adenovirus-ad-cmv-crma-bhv21600014","title":"CrmA Adenovirus (Ad-CMV-CrmA)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-CrmA is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the CrmA gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e CrmA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eCytokine response modifier A\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying Caspase Inhibitors.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1032-crma-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286487720301,"sku":"1032","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"apc-adenovirus-ad-cbr-bhv21600044","title":"APC Adenovirus (Ad-CBR)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CBR is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the CBR gene with a eGFP epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e CBR (eGFP tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAdenomatous polyposis coli gene (APC) is a tumor suppressor gene located on chromosome 5q2. It is inactivated in most colorectal tumors. APC is a cytoplasmic protein that can bind to and promote the degradation of b-catenin. In most colorectal tumors, APC is inactivated. This increases the interaction between b-catenin and Tcf family of transcription factors, leading to increase transcription of genes such as c-Myc.\u003c\/p\u003e\u003cp\u003eThis adenovirus contains APC mini-me fragment.\u003c\/p\u003e\u003cp\u003eShih IM., et al., Cancer Res. 2000 Mar 15;60(6):1671-6.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying TGF-β \/ WNT.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1283-apc-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286487851373,"sku":"1283","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"akt1-adenovirus-ad-cmv-akt1-wt-bhv21600004","title":"Akt1 Adenovirus (Ad-CMV-Akt1 (wt))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Akt1 (wt) is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Akt1 (wt) gene with a HA epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Akt1 (wt) (HA tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAn HA tag was fused in frame to the N termini of the mouse Akt1 coding sequences.\u003c\/p\u003e\u003cp\u003eAkt1\/PKB is activated in response to many extracellular signals and play key roles in regulating diverse cellular processes, including cell proliferation, differentiation, apoptosis, angiogenesis and glycogen synthesis etc. Akt1 is activated by the phosphorylation of the T loop of their kinase domain by PDK1 and by phosphorylation of a residue located C-terminal to the kinase domain in the hydrophobic motif.\u003c\/p\u003e\u003cp\u003eThe Akt family of kinases includes Akt1, Akt2 and Akt3.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying PI3K\/AKT Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1022-akt1-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286487916909,"sku":"1022","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"akt2-myr-adenovirus-ad-cmv-akt2-myr-bhv21600005","title":"Akt2 (Myr) Adenovirus (Ad-CMV-Akt2 (Myr))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Akt2 (Myr) is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Akt2 (Myr) gene with a HA epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Akt2 (Myr) (HA tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eHA-Akt2 is constructed by fusing the HA tag in frame to the N termini of the coding sequence of wild type Akt2. To construct Myr-HA-Akt2, the c-Src myristoylation sequence was fused in frame to the N terminus of the HA-Akt2 (wild-type) coding sequence. Myr-HA-Akt2 is a constitutively active mutant of Akt2, and it is also referred as Akt2(ca).\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying PI3K\/AKT Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1023-akt2-myr-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286487949677,"sku":"1023","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"akt1-dn-adenovirus-ad-cmv-akt1-dn-bhv21600003","title":"Akt1 (dn) Adenovirus (Ad-CMV-Akt1 (dn))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Akt1 (dn) is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Akt1 (dn) gene with a HA epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Akt1 (dn) (HA tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAn HA tag was fused in frame to the N termini of the coding sequence of mouse Akt1(T308A, S473A) mutant, which is also known as Akt1(AA).\u003c\/p\u003e\u003cp\u003eAkt1\/PKB is activated in response to many extracellular signals and play key roles in regulating diverse cellular processes, including cell proliferation, differentiation, apoptosis, angiogenesis and glycogen synthesis etc. Akt1 is activated by the phosphorylation of the T loop of their kinase domain by PDK1 and by phosphorylation of a residue located C-terminal to the kinase domain in the hydrophobic motif.\u003c\/p\u003e\u003cp\u003eThe Akt family of kinases includes Akt1, Akt2 and Akt3.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying PI3K\/AKT Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1021-akt1-dn-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286487982445,"sku":"1021","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"akt1myr-adenovirus-ad-cmv-akt1-myr-bhv21600002","title":"Akt1(Myr) Adenovirus (Ad-CMV-Akt1 (Myr))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Akt1 (Myr) is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Akt1 (Myr) gene with a HA epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Akt1 (Myr) (HA tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eHA-Akt1 is constructed by fusing the HA tag in frame to the N termini of the coding sequence of wild type Akt1. To construct Myr-HA-Akt1, the c-Src myristoylation sequence was fused in frame to the N terminus of the HA-Akt (wild-type) coding sequence. Myr-HA-Akt1 is a constitutively active mutant of Akt1, and it is also referred as Akt(ca).\u003c\/p\u003e\u003cp\u003eAkt1\/PKB is activated in response to many extracellular signals and play key roles in regulating diverse cellular processes, including cell proliferation, differentiation, apoptosis, angiogenesis and glycogen synthesis etc. Akt1 is activated by the phosphorylation of the T loop of their kinase domain by PDK1 and by phosphorylation of a residue located C-terminal to the kinase domain in the hydrophobic motif.\u003c\/p\u003e\u003cp\u003eThe Akt family of kinases includes Akt1, Akt2 and Akt3.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying PI3K\/AKT Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1020-akt1myr-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488146285,"sku":"1020","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"c-ras-adenovirus-ad-cmv-c-ras-bhv21600012","title":"c-Ras Adenovirus (Ad-CMV-c-Ras)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-c-Ras is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the c-Ras gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e c-Ras.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003ec-Ras is delivered to target cells via adenoviral transduction. Adenoviral delivery results in episomal (non-integrating) expression with rapid onset (typically detectable by 24 hours, peaking at 48–72 hours).\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying RAS\/RAF\/MEK\/ERK Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1030-c-ras-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488211821,"sku":"1030","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"akt2-adenovirus-ad-cmv-akt2-wt-bhv21600006","title":"Akt2 Adenovirus (Ad-CMV-Akt2 (wt))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Akt2 (wt) is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Akt2 (wt) gene with a HA epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Akt2 (wt) (HA tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe Akt family of kinases includes Akt1, Akt2 and Akt3.\u003c\/p\u003e\u003cp\u003eAn HA tag was fused in frame to the N termini of the mouse Akt2 coding sequences.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying PI3K\/AKT Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1024-akt2-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488277357,"sku":"1024","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"ras-n17-adenovirus-ad-cmv-ras-n17-bhv21600013","title":"Ras (N17) Adenovirus (Ad-CMV-Ras (N17))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Ras (N17) is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Ras (N17) gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Ras (N17).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eRas genes encode 21 kDa guanine nucleotide-binding proteins, including H-, K- and N-Ras. H-Ras was first identified as oncogene, mutated Ras genes have been found in many human tumors. Like all GTPases, Ras act as molecular switch to control downstream cellular events. The interconversion of the inactive GDP-bound form into the active GTP-bound form is regulated by guanine nucleotide exchange factors, whereas inactivation of the GTP-bound form is stimulated by GTPase-activating proteins (GAPs). Ras in its active GTP bound form binds to Raf, resulting in activation of MAP kinase cascade. The provided recombinant adenovirus contains dominant negative form of human H-Ras (T17N).\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying RAS\/RAF\/MEK\/ERK Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1031-ras-n17-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488310125,"sku":"1031","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"c-myc-adenovirus-ad-c-myc-bhv21600046","title":"c-Myc Adenovirus (Ad-c-Myc)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-c-Myc is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the c-Myc gene with a eGFP epitope tag under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e c-Myc (eGFP tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003ec-Myc is a very strong proto-oncogene and it is very often found to be upregulated in many types of cancers. It is a mammalian transcription factor belonging to the bHLH (basic Helix Loop Helix)-Leucine Zipper family. The Myc family of cellular oncogenes, which includes c-Myc, N-Myc, L-Myc, S-Myc and B-Myc, represents nuclear transcription factors that play a significant role in cellular proliferation, differentiation, apoptosis and transformation. Members of this gene family are activated upon various mitogenic signals such as Wnt, Shh and EGF. They form heteromeric complexes with other interacting partners, such as members of the Max and Mad families, to activate transcription. Alternatively, c-Myc can also inhibit the DNA binding protein Miz-1 to repress a second set of target genes. Mnt and Mlx regulate Myc activity by forming heterodimers with Max or Mad, respectively, to suppress Myc-induced transcriptional activation.\u003c\/p\u003e\u003cp\u003eThis adenovirus contain human c-Myc. It was constructed using AdEasy system.\u003c\/p\u003e\u003cp\u003eReference: Hermeking H., et al., Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2229-34.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying MYC Family.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1285-c-myc-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488441197,"sku":"1285","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fas-ligand-adenovirus-ad-cmv-fasl-bhv21600011","title":"Fas Ligand Adenovirus (Ad-CMV-FasL)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-FasL is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the FasL gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e FasL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThis catalog number has been changed to #1594. Please go to the product page for cat#1594 for detail.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying Death Receptors.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1029-fas-ligand-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488506733,"sku":"1029","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fas-ligand-tet-on-adenovirus-ad-tet-on-fasl-bhv21600037","title":"Fas Ligand (tet-on) Adenovirus (Ad-Tet-on-FasL)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-Tet-on-FasL is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the on-FasL gene under the Tet-on promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (Tet-on):\u003c\/strong\u003e a doxycycline-inducible promoter system requiring tetracycline transactivator co-expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e on-FasL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe FasL transgene is under the control of a tetracycline-regulated promoter.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying Death Receptors.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1200-fas-ligand-tet-on-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488539501,"sku":"1200","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"brca1-adenovirus-ad-brca1-bhv21600054","title":"BRCA1 Adenovirus (Ad-BRCA1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-BRCA1 is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the BRCA1 gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e BRCA1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe breast cancer susceptibility gene, BRCA1, which maps to chromosome 17q is expressed in numerous tissues, including breast and ovary. A second breast cancer susceptibility gene, BRCA2, located on chromosome 13q12-13, also confers a high incidence of breast and ovarian cancer. Mutations within the BRCA1 gene may account for at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer.\u003c\/p\u003e\u003cp\u003eThis adenovirus expresses the human BRCA1 transcript variant 5 (NM_007299).  This variant differs in the 5′ UTR, uses two alternate in-frame splice sites in the central coding region, and lacks an alternate exon in the 3′ coding region that results in a frameshift, compared to variant 1. So this variant is shorter and has a distinct C-terminus, compared to BRCA1 isoform 1.\u003c\/p\u003e\u003cp\u003ePlease contact us if you need adenoviruses expressing other isoforms of human BRCA1.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying DNA Repair.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1351-brca1-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488572269,"sku":"1351","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"rb-adenovirus-ad-cmv-rb-bhv21600018","title":"Rb Adenovirus (Ad-CMV-Rb)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-Rb is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the Rb gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e Rb.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe retinoblastoma susceptibility protein Rb is a 110 kDa phosphorylated protein. Like p53, Rb p110 is an anti-oncogene that is subject to inactivation by either mutation or by binding to certain DNA tumor virus-encoded proteins. Rb binds to and regulates transcription factors involved in cell cycle regulation, including the E2F family. Two Rb related proteins, p107 and p130, also function to regulate specific members of the E2F transcription factor family. Binding and inactivation of E2F proteins by Rb is regulated by cyclin dependent kinase-mediated phosphorylation\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying Rb \/ Cell Cycle.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1043-rb-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488605037,"sku":"1043","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"p53-adenovirus-ad-p53-gfp-bhv21600040","title":"p53 Adenovirus (Ad-p53-GFP)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-p53-GFP is a replication-defective recombinant Ad5 adenovirus expressing the GFP reporter gene under the p53 promoter. Reporter adenoviruses are commonly used to benchmark transduction efficiency, titrate MOI in new cell or animal models, and as transduction-load controls for paired over-expression vectors.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (p53):\u003c\/strong\u003e a p53-responsive promoter element.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e GFP (eGFP tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe widely studied p53 tumor suppressor gene contains mutations in over 50% of human cancers. p53 protein expression is low in normal cells but increases in response to DNA damage and cellular distress signals. Overexpression of the p53 transcription factor can induce either cell cycle arrest or apoptosis through transcriptional regulation of several genes, including the cell cycle inhibitor p21, DNA repair gene GADD45 and the apoptotic inducer Bax. p53 also induces apoptosis by means of a direct signaling pathway involving the expression of p53AIP1. p53 directly binds to and acts on several cellular proteins involved in various pathways, including c-Abl, basal transcription factor TFIIH and WT1. p53 can be functionally inactivated by mutation, binding to DNA tumor virus encoded proteins, such as SV40 large T antigen, Adenovirus E1B and papilloma virus E6 proteins, or through its interaction with MDM2.\u003c\/p\u003e\u003cp\u003eThis adenovirus express both human p53 and GFP.\u003c\/p\u003e\u003cp\u003eReference: Yu J., et al., PNAS (1999) 96: 14517; Chan TA., et al., Genes Dev (00) 14:1584\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying p53 Pathway.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eTransduction-efficiency benchmarking in new cell lines or animal models.\u003c\/li\u003e\n\u003cli\u003eMOI titration as a positive control before switching to a transgene of interest.\u003c\/li\u003e\n\u003cli\u003ePromoter-activity readout for transcriptional studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1260-p53-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488899949,"sku":"1260","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"p21-cdkn1a-adenovirus-ad-cmv-p21-bhv21600016","title":"p21\/CDKN1A Adenovirus (Ad-CMV-p21)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-CMV-p21 is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the p21 gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e p21.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003ep21\/CDKN1A is a cyclin-dependent kinase inhibitor. It is a key effector of cellular senescence downstream of the tumor suppressor p53. p21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of p21\/CDKN1A is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying CDK Inhibitors.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1041-p21-cdkn1a-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286488965485,"sku":"1041","price":475.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.ebiohippo.com\/collections\/rt-cancer-vectors-viruses.oembed?page=2","provider":"BioHippo","version":"1.0","type":"link"}