{"title":"Angiogenesis \u0026 Tumor Vasculature — Vectors \u0026 Viruses","description":null,"products":[{"product_id":"cell-cycle-reporter-lentivirus-bhv19400048","title":"Cell Cycle Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCell cycle progression through the G1, S, G2, and M phases is tightly coordinated to ensure faithful DNA replication and division. A key mechanism of phase control is regulated protein degradation: the licensing factor CDT1 accumulates in G1 and is degraded as cells enter S phase, while Geminin accumulates during S, G2, and M and is degraded at the end of mitosis. The fluorescent ubiquitination-based cell cycle indicator (FUCCI) exploits these reciprocal oscillations by fusing fluorescent proteins to fragments of CDT1 and Geminin, allowing real-time visualization of cell cycle phase in living cells. This approach is widely used in studies of proliferation, differentiation, stem cell biology, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Cell Cycle Reporter Lentivirus delivers a FUCCI-based system for visualizing cell cycle progression in living cells without fixation. Human CDT1 (amino acids 30-120), which accumulates in G1, is fused to RFP, and human Geminin (amino acids 1-110), which accumulates in G2\/M, is fused to GFP. A CMV promoter drives RFP-CDT1, GFP-Geminin, and a drug selection marker as a single transcript, with self-cleaving T2A and P2A sequences yielding three separate proteins for balanced reporter expression.\u003c\/p\u003e\n\u003cp\u003eThis arrangement enables precise monitoring of all cell cycle phases in stable or transiently transduced cells. Supplied as VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product suits a wide range of mammalian cells, including primary, thawed, and pluripotent stem cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, GFP, hCDT1-RFP + hGemn-GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Cell cycle (phases) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615326573,"sku":"LTV-0052-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251635347821,"sku":"LTV-0052-2S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FUCCI-Puro-Fig.1.jpg?v=1776934330"},{"product_id":"p53-reporter-lentivirus-bhv19400007","title":"P53 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTP53 encodes p53, a tumor suppressor transcription factor often described as the guardian of the genome. In response to cellular stresses such as DNA damage, oncogene activation, and hypoxia, p53 is stabilized and binds specific response elements to activate genes that drive cell cycle arrest, DNA repair, senescence, and apoptosis. Through this program, p53 prevents the propagation of damaged or potentially malignant cells. TP53 is the most frequently mutated gene in human cancer, and loss of p53 function compromises these protective responses. Because of its central role, p53 transcriptional activity is widely monitored in studies of genotoxic stress, apoptosis, and tumor suppression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe P53 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of p53 activity in human or mouse cells. Tandem consensus p53 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, or Renilla luciferase), so that p53 activation produces a quantitative signal indicative of p53 and apoptosis pathway engagement in transduced cells. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study genotoxic stress responses, apoptosis, and tumor suppressor signaling, and to screen modulators of p53 activity. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Apoptosis\/p53 signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615621485,"sku":"LTV-0008-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893982061,"sku":"LTV-0008-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310894014829,"sku":"LTV-0008-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894047597,"sku":"LTV-0008-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310894080365,"sku":"LTV-0008-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894113133,"sku":"LTV-0008-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_b476d082-2641-436d-80a2-b5c8f467b270.jpg?v=1776934151"},{"product_id":"foxc2-ets-reporter-lentivirus-bhv19400227","title":"FOXC2\/ETS Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXC2 is a forkhead box transcription factor essential for developmental patterning and tissue maintenance, including lymphatic vessel formation and the development of the lungs, eyes, kidneys, urinary tract, and cardiovascular system. ETS family transcription factors, such as the endothelial regulator ETV2, control endothelial and mesodermal gene programs. FOXC2 and ETS factors can act together at composite regulatory elements, where their synergistic binding drives target genes involved in vascular and lymphatic development. This cooperative FOXC2-ETS activity is important for endothelial differentiation and has relevance to angiogenesis, lymphangiogenesis, and developmental disorders, making the combined transcriptional output an informative readout in vascular biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXC2\/ETS Reporter Lentivirus is a transcription-factor reporter system that measures the combined activity of the forkhead factor FOXC2 and the ETS family member ETV2. The construct uses tandem repeats of FOXC2 binding elements derived from the MEF2 enhancer to detect their synergistic transcriptional activity and the activation of related signaling pathways. A fluorescent or luminescent reporter, chosen from options including GFP, RFP, mCherry, BFP2, and luciferase configurations, is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying endothelial and lymphatic development and FOXC2-ETS cooperation. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXC2 \u0026amp; ETS family members synergistic activity transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616244077,"sku":"LTV-0118-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362029789549,"sku":"LTV-0118-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362029822317,"sku":"LTV-0118-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362029855085,"sku":"LTV-0118-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362029887853,"sku":"LTV-0118-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362029920621,"sku":"LTV-0118-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXC2-ETV2-TAG-PURO-FIG1-1.jpg?v=1776934147"},{"product_id":"c-myc-reporter-lentivirus-bhv19400006","title":"c-MYC Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ec-MYC is a basic helix-loop-helix leucine zipper transcription factor and a master regulator of cell growth, proliferation, metabolism, and ribosome biogenesis. By dimerizing with MAX and binding E-box elements in target promoters, c-MYC activates and represses large gene networks that coordinate the cell cycle and biosynthetic capacity. Its expression is tightly controlled in normal cells, but c-MYC is one of the most commonly deregulated oncogenes in human cancer. Aberrant c-MYC activity drives uncontrolled proliferation, metabolic reprogramming, and tumor progression, making it a central focus of oncology and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe c-MYC Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of c-MYC transcriptional activity in human or mouse cells. Reporter expression is controlled by c-MYC-responsive elements, so signal intensity reflects endogenous c-MYC activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study c-MYC-driven proliferation, metabolism, and transformation, and to screen modulators of c-MYC pathway activity. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the cMYC transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616866669,"sku":"LTV-0007-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896537965,"sku":"LTV-0007-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896570733,"sku":"LTV-0007-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896603501,"sku":"LTV-0007-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896636269,"sku":"LTV-0007-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896669037,"sku":"LTV-0007-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0007-Fig1-MYC-TAG-Puro-1.jpg?v=1776934330"},{"product_id":"h-m-hif2a-shrna-lentivirus-bhv19400124","title":"h\/m HIF2a shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that drives cellular adaptation to low oxygen. Under normoxia it is hydroxylated and degraded; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a gene set partly distinct from HIF-1α. Aberrant HIF-2α activity contributes to clear cell renal carcinoma and other tumors, where it promotes growth and vascularization, making EPAS1 an important target in cancer and hypoxia research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m HIF2a shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse EPAS1 (HIF2a) for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of hypoxia signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting EPAS1 (NCBI Accession: NM_001430) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251617259885,"sku":"LSV-0005-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311258427757,"sku":"LSV-0005-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311258460525,"sku":"LSV-0005-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311258493293,"sku":"LSV-0005-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311258526061,"sku":"LSV-0005-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311258558829,"sku":"LSV-0005-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311258591597,"sku":"LSV-0005-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311258624365,"sku":"LSV-0005-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311258657133,"sku":"LSV-0005-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311258689901,"sku":"LSV-0005-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0005-hm-HIF2a-sh-2-sh3.jpg?v=1776934211"},{"product_id":"hif1a-reporter-lentivirus-bhv19400005","title":"HIF1a Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 1 alpha (HIF-1α), encoded by HIF1A, is an oxygen-sensitive transcription factor that orchestrates the cellular response to low oxygen. Under normoxia, HIF-1α is hydroxylated and rapidly degraded; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate genes controlling glycolysis, angiogenesis, and erythropoiesis. HIF-1α drives metabolic adaptation in hypoxic tissues and tumors, where it promotes survival, vascularization, and aggressive behavior. HIF-1α is a major focus of oncology, angiogenesis, and hypoxia research, and is mechanistically distinct from the related factor HIF-2α.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF1a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-1α activity. The construct is preferentially activated by HIF-1α rather than HIF-2α, supporting discrimination between the two factors. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring hypoxia pathway activation and screening HIF-1α modulators in oncology and angiogenesis research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia\/HIF pathway (HIF-1a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617292653,"sku":"LTV-0006-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310894866797,"sku":"LTV-0006-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894899565,"sku":"LTV-0006-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310894932333,"sku":"LTV-0006-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310894965101,"sku":"LTV-0006-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894997869,"sku":"LTV-0006-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HRE-TAL-Puro-IVIS.jpg?v=1776934147"},{"product_id":"hif2a-reporter-lentivirus-bhv19400025","title":"HIF2a Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that mediates cellular adaptation to low oxygen. Under normoxia, HIF-2α is hydroxylated and targeted for degradation; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a tissue distribution and gene set partly distinct from HIF-1α. Dysregulated HIF-2α activity contributes to clear cell renal carcinoma and other tumors, making it an important target in oncology, angiogenesis, and hypoxia research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF2a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-2α (EPAS1) activity in transduced cells. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Purified by PEG precipitation and sucrose gradient centrifugation, the particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring hypoxia pathway activation, screening HIF-2α modulators, and studying angiogenesis and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia\/HIF pathway (HIF-2a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617358189,"sku":"LTV-0029-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310891426157,"sku":"LTV-0029-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310891458925,"sku":"LTV-0029-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891491693,"sku":"LTV-0029-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310891524461,"sku":"LTV-0029-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310891557229,"sku":"LTV-0029-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891589997,"sku":"LTV-0029-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_78b405ca-f48e-425b-a811-ae56b18e0bdd.jpg?v=1776934275"},{"product_id":"foxm1-reporter-lentivirus-bhv19400055","title":"FOXM1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXM1 is a member of the Forkhead box family of transcription factors, which share a conserved DNA-binding domain but differ in flanking sequences that confer target specificity. FOXM1 is a master regulator of cell cycle progression, controlling gene expression required for the G2 and M phases, including genes for mitotic entry and chromosome segregation. FOXM1 activity is tied to proliferation, DNA damage repair, and cellular senescence. It is overexpressed in many cancers, where it drives uncontrolled proliferation and tumor progression, making FOXM1 a prominent target for cancer therapy and a focus of oncology and developmental biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXM1 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of FOXM1 DNA-binding elements designed to preferentially read out FOXM1 activity over other Forkhead transcription factors. The elements drive a reporter gene downstream of a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXM1 transcriptional activity and associated cell cycle signaling. Reporter options include EGFP, GFP, mCherry, RFP, d2GFP, and firefly luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying cell cycle regulation and cancer biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the FOXM1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617423725,"sku":"LTV-0059-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893097325,"sku":"LTV-0059-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310893130093,"sku":"LTV-0059-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893162861,"sku":"LTV-0059-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310893195629,"sku":"LTV-0059-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310893228397,"sku":"LTV-0059-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0059-FOXM1-Reporter-Fig1.jpg?v=1776934148"},{"product_id":"e2f-reporter-lentivirus-bhv19400022","title":"E2F Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eE2F transcription factors are central regulators of the cell cycle, controlling the transition from G1 to S phase. By binding consensus E2F DNA elements, activator E2Fs such as E2F1 induce genes required for DNA replication and proliferation, including cyclins A and E. E2F activity is governed by the retinoblastoma protein (Rb): in G0 and early G1, hypophosphorylated Rb binds E2F and represses its target genes, while cyclin-dependent kinases phosphorylate Rb to release active E2F. This Rb-E2F axis is one of the most frequently disrupted pathways in cancer, and E2F overexpression promotes tumor development in tissues such as breast, lung, and liver, making E2F activity an important oncology research readout.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe E2F Reporter Lentivirus places a reporter gene under the control of tandem consensus E2F DNA-binding elements, providing a sensitive readout of E2F transcription factor activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring cell cycle progression and Rb-E2F pathway activity, studying proliferation in cancer models, and screening cell cycle modulators.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the E2F transcription factors transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617882477,"sku":"LTV-0026-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310900863341,"sku":"LTV-0026-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310900896109,"sku":"LTV-0026-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310900928877,"sku":"LTV-0026-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310900961645,"sku":"LTV-0026-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310900994413,"sku":"LTV-0026-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310901027181,"sku":"LTV-0026-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/E2F-Reporter-Fig.-2.jpg?v=1776934143"},{"product_id":"erg-reporter-lentivirus-bhv19400090","title":"ERG Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eERG (ETS-related gene) is a transcription factor of the ETS family that recognizes a core GGAA DNA-binding motif with preferences for specific flanking sequences. ERG regulates embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis, and is an important regulator of endothelial and hematopoietic cell function. ERG often cooperates with the AP-1 transcription factor complex for optimal transcriptional activity. Chromosomal translocations involving ERG generate oncogenic fusion proteins, including TMPRSS2-ERG in prostate cancer, EWS-ERG in Ewing sarcoma, and FUS-ERG in acute myeloid leukemia, making ERG transcriptional activity an important readout in oncology and vascular biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ERG Reporter Lentivirus places a reporter gene under the control of consensus ERG DNA-binding elements that include an AP-1-like motif, providing a sensitive readout of ERG transcriptional activity in human and mouse cells. A broad range of reporter options is available, including fluorescent (GFP, EGFP, d2GFP, BFP2, mCherry, RFP), luminescent (firefly, Renilla, and Gaussia luciferase), and combined formats, with blasticidin, hygromycin, puromycin, or zeocin selection.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry. Applications include monitoring ERG activity in cancer and endothelial models.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the ERG transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251618406765,"sku":"LTV-0099-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251632431469,"sku":"LTV-0099-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53251632464237,"sku":"LTV-0099-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53251632497005,"sku":"LTV-0099-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53251632529773,"sku":"LTV-0099-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53251632562541,"sku":"LTV-0099-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Puromycin \/ 5x10^6","offer_id":53251632595309,"sku":"LTV-0099-15S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Blasticidin \/ 5x10^6","offer_id":53251632628077,"sku":"LTV-0099-16S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ERG-TAG-Puro-Thumbnail-1.jpg?v=1776934145"},{"product_id":"h-m-hif1a-shrna-lentivirus-bhv19400123","title":"h\/m HIF1a shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHIF1A encodes hypoxia-inducible factor 1-alpha, the oxygen-sensitive subunit of the HIF-1 transcription factor that coordinates the cellular response to low oxygen. Under normal oxygen levels, prolyl hydroxylases modify HIF-1α, marking it for von Hippel-Lindau-mediated degradation. Under hypoxia, this degradation is suppressed, allowing HIF-1α to dimerize with HIF-1β and activate genes that promote angiogenesis, glycolysis, erythropoiesis, and metabolic adaptation. HIF-1 signaling is essential for development and ischemic tissue responses, and it is frequently hyperactivated in solid tumors, where it drives angiogenesis, metabolic reprogramming, and resistance to therapy. HIF-1α is therefore a key focus of cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m HIF1a shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse HIF1A for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of HIF1A using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of hypoxia signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting HIF1A (NCBI Accession: NM_001530) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"None \/ Blasticidin \/ 2x10^6","offer_id":53251618505069,"sku":"LSV-0004-T2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 2x10^6","offer_id":53311255085421,"sku":"LSV-0004-T1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311255118189,"sku":"LSV-0004-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311255150957,"sku":"LSV-0004-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255183725,"sku":"LSV-0004-4-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255216493,"sku":"LSV-0004-4-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311255249261,"sku":"LSV-0004-4-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311255282029,"sku":"LSV-0004-4-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255314797,"sku":"LSV-0004-4-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255347565,"sku":"LSV-0004-4-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255380333,"sku":"LSV-0004-4-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255413101,"sku":"LSV-0004-4-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0004-hm-HIF1a-sh-Fig1.jpg?v=1776934150"},{"product_id":"hif1a-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400096","title":"HIF1A Wild Type \u0026 Mutant ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHIF1A encodes hypoxia-inducible factor 1-alpha, the oxygen-sensitive subunit of the HIF-1 transcription factor that orchestrates the cellular response to low oxygen. Under normoxia, prolyl hydroxylases modify conserved proline residues in HIF-1α, marking it for von Hippel-Lindau-mediated ubiquitination and proteasomal degradation. Under hypoxia, this degradation is blocked, allowing HIF-1α to dimerize with HIF-1β and activate genes governing angiogenesis, glycolysis, erythropoiesis, and metabolic adaptation. HIF-1 signaling is central to development, ischemic disease, and tumor biology, where it drives angiogenesis and metabolic reprogramming. Mutating the regulatory proline and asparagine residues (P402A\/P577A\/N813A) yields a stabilized protein that remains active under normoxic conditions.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF1A Wild Type \u0026amp; Mutant ORF cDNA Lentivirus delivers the human or mouse HIF1A open reading frame for stable overexpression in mammalian cells, available in wild-type form and as a normoxia-stable mutant (P402A\/P577A\/N813A) resistant to oxygen-mediated degradation. The ORF is fused to a C-terminal V5 tag and linked via self-cleaving peptides to a fluorescent reporter (GFP or RFP) or luciferase and a drug-selection marker (puromycin or blasticidin); expression is driven by a CMV or EF1a promoter, with PGK driving reporter and selection cassettes in some constructs. The particles efficiently transduce difficult-to-transfect cells, including primary and thawed cultures, supporting stable cell line generation for studies of hypoxia signaling, angiogenesis, and tumor metabolism. ORF integrity is verified by sequencing and expression confirmed by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of HIF1A (NCBI Accession: NM_001530, NM_010431) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-HIF1A-V5 \/ GFP\/none \/ 2x10^6","offer_id":53251619094893,"sku":"LCV-0001-H8","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ RFP\/Blasticidin \/ 2x10^6","offer_id":53311259672941,"sku":"LCV-0001-H6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ GFP\/none \/ 2x10^6","offer_id":53311259705709,"sku":"LCV-0001-H5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ none\/Blasticidin \/ 2x10^6","offer_id":53311259738477,"sku":"LCV-0001-H7","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a-V5 \/ GFP\/none \/ 2x10^6","offer_id":53311259771245,"sku":"LCV-0001-M5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ GFP\/none \/ 2x10^6","offer_id":53311259804013,"sku":"LCV-0001-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ RFP\/none \/ 2x10^6","offer_id":53311259836781,"sku":"LCV-0001-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ none\/Puromycin \/ 2x10^6","offer_id":53311259869549,"sku":"LCV-0001-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ none\/Blasticidin \/ 2x10^6","offer_id":53311259902317,"sku":"LCV-0001-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"n-myc-reporter-lentivirus-bhv19400228","title":"N-MYC Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eN-MYC (MYCN) is a basic helix-loop-helix-leucine-zipper transcription factor of the MYC family. It heterodimerizes with its partner protein MAX and binds E-box DNA sequences to activate transcription of genes that drive cell growth, proliferation, ribosome biogenesis, and metabolism. During development, N-MYC is expressed in a lineage-specific manner and is essential for central nervous system formation and the proliferation of neural progenitor cells. Amplification or deregulated expression of MYCN is a well-established oncogenic event in aggressive childhood tumors, most notably neuroblastoma and medulloblastoma. Reporter systems with E-box response elements provide a quantitative readout of N-MYC transcriptional output for studies of development and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe N-MYC Reporter Lentivirus is a transcription-factor reporter system that detects N-MYC-driven transcriptional activity in mammalian cells. The construct uses tandem consensus E-box motifs with optimized flanking sequences to read out N-MYC activity selectively, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines and flexible detection by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and are suited to difficult-to-transfect cells, supporting research on N-MYC in CNS development, lineage specification, and oncogenesis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the N-MYC transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"N-MYC-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251622699373,"sku":"LTV-0120-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087100781,"sku":"LTV-0120-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362087133549,"sku":"LTV-0120-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362087166317,"sku":"LTV-0120-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087199085,"sku":"LTV-0120-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362087231853,"sku":"LTV-0120-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362087264621,"sku":"LTV-0120-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362087297389,"sku":"LTV-0120-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362087330157,"sku":"LTV-0120-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362087362925,"sku":"LTV-0120-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"N-MYC-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362087395693,"sku":"LTV-0120-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/N-MYC-Reporter-Figure.jpg?v=1776934149"},{"product_id":"h-m-nrp1-shrna-lentivirus-bhv19400169","title":"h\/m NRP1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuropilin 1 (NRP1) is a single-pass transmembrane glycoprotein that functions as a co-receptor for multiple ligand families, including class 3 semaphorins and members of the VEGF family. By partnering with plexins and VEGF receptors, NRP1 modulates axon guidance, vascular development, and angiogenesis. NRP1 is also expressed on immune cells, where it influences regulatory T cell function and dendritic cell activity. In cancer, NRP1 promotes tumor angiogenesis, cell migration, and invasion, and contributes to an immunosuppressive microenvironment. These roles make NRP1 a focus of research in vascular biology, neurodevelopment, immunology, and oncology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m NRP1 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse NRP1 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% NRP1 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option supplies a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of angiogenesis, axon guidance, immune regulation, and tumor progression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting NRP1 (NCBI Accession: NM_003873.7) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251622961517,"sku":"LSV-0053-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311268061549,"sku":"LSV-0053-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311268094317,"sku":"LSV-0053-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311268127085,"sku":"LSV-0053-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311268159853,"sku":"LSV-0053-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311268192621,"sku":"LSV-0053-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311268225389,"sku":"LSV-0053-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311268258157,"sku":"LSV-0053-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311268290925,"sku":"LSV-0053-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311268323693,"sku":"LSV-0053-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0053-hm-NRP1-sh-FIG1.jpg?v=1776934279"},{"product_id":"pdgfd-orf-cdna-lentivirus-bhv19400098","title":"PDGFD ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGF-D (platelet-derived growth factor D) is a member of the PDGF family of growth factors. It is secreted as a latent protein that requires proteolytic removal of its CUB domain to release the active growth-factor domain, which then signals primarily through the PDGF receptor beta. PDGF-D signaling stimulates the proliferation, migration, and survival of mesenchymal cells such as fibroblasts and smooth muscle cells, and contributes to angiogenesis, wound healing, and tissue remodeling. Aberrant PDGF-D activity has been linked to fibrosis, atherosclerosis, and tumor progression, where it can promote a supportive microenvironment, making PDGF-D a target of interest in cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PDGFD ORF cDNA Lentivirus delivers the open reading frame of human PDGF-D (transcript variant 1) for stable overexpression in mammalian cells. A CMV promoter drives expression of PDGF-D fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and a drug selection marker (puromycin or blasticidin) separated from the cDNA by self-cleaving peptides for independent translation. In some configurations a PGK promoter drives the reporter and selection marker.\u003c\/p\u003e\n\u003cp\u003eThe vectors are optimized for high expression and reliable genome integration, enabling long-term stable cell lines. ORF cloning accuracy is confirmed by sequencing and protein expression validated by transient transfection. Particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of PDGFD (NCBI Accession: NM_025208) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Puromycin, Blasticidin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-PDGFD-V5\/GFP \/ RFP \/ 5x10^6","offer_id":53251623059821,"sku":"LCV-0003-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-V5\/RFP \/ RFP \/ 5x10^6","offer_id":53311264424301,"sku":"LCV-0003-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-Flag\/Puro \/ N\/A \/ 5x10^6","offer_id":53311264457069,"sku":"LCV-0003-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-Flag\/BSD \/ N\/A \/ 5x10^6","offer_id":53311264489837,"sku":"LCV-0003-4S","price":595.0,"currency_code":"USD","in_stock":true}]},{"product_id":"human-pdgfb-shrna-lentivirus-bhv19400158","title":"Human PDGFB shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGFB encodes the platelet-derived growth factor subunit B, a polypeptide that assembles into PDGF-BB homodimers and PDGF-AB heterodimers. Secreted PDGF ligands signal through PDGF receptor tyrosine kinases to stimulate the proliferation, migration, and survival of mesenchymal cells such as fibroblasts, smooth muscle cells, and pericytes. PDGF-B signaling is essential for blood vessel maturation and pericyte recruitment, wound healing, and connective tissue formation. Dysregulated PDGFB expression contributes to fibrosis, vascular disease, and tumor growth, where it promotes angiogenesis and an activated tumor stroma, making PDGFB an important target in cancer and cardiovascular research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PDGFB shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human platelet-derived growth factor subunit B (PDGFB). The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of PDGF-B signaling in cell proliferation, angiogenesis, fibrosis, and tumor stroma biology. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting PDGFB (NCBI Accession: NM_002608.4) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623256429,"sku":"LSV-0042-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311258722669,"sku":"LSV-0042-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311258755437,"sku":"LSV-0042-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311258788205,"sku":"LSV-0042-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311258820973,"sku":"LSV-0042-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311258853741,"sku":"LSV-0042-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311258886509,"sku":"LSV-0042-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311258919277,"sku":"LSV-0042-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311258952045,"sku":"LSV-0042-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311258984813,"sku":"LSV-0042-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0042-h-PDGFB-sh-fig1.jpg?v=1776934279"},{"product_id":"h-m-nrp2-shrna-lentivirus-bhv19400161","title":"h\/m NRP2 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuropilin 2 (NRP2) is a single-pass transmembrane glycoprotein that functions as a co-receptor for class 3 semaphorins and for members of the vascular endothelial growth factor (VEGF) family. By modulating semaphorin and VEGF signaling, NRP2 regulates axon guidance, lymphatic and vascular development, and cell migration. NRP2 is also expressed on immune cells and tumor cells, where it influences cell adhesion, motility, and survival. Aberrant NRP2 expression contributes to tumor progression, lymphangiogenesis, and metastasis in several cancers, making it an actively investigated target in cancer biology, angiogenesis research, and neurodevelopmental studies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m NRP2 shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human and mouse Neuropilin 2. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of NRP2 in tumor progression, lymphangiogenesis, cell migration, and semaphorin\/VEGF signaling. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting NRP2 (NCBI Accession: NM_201266.2) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623354733,"sku":"LSV-0045-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311265571181,"sku":"LSV-0045-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311265603949,"sku":"LSV-0045-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311265636717,"sku":"LSV-0045-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311265669485,"sku":"LSV-0045-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311265702253,"sku":"LSV-0045-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311265735021,"sku":"LSV-0045-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311265767789,"sku":"LSV-0045-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311265800557,"sku":"LSV-0045-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311265833325,"sku":"LSV-0045-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0045-hm-NRP2-sh-fig1-11-22.jpg?v=1776934279"},{"product_id":"human-pdgfd-shrna-lentivirus-bhv19400136","title":"Human PDGFD shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGFD encodes platelet-derived growth factor D, a member of the PDGF family of growth factors. PDGF-D is secreted as a latent dimer that is proteolytically activated and signals primarily through the PDGF receptor beta to drive proliferation, migration, and survival of mesenchymal cells, including fibroblasts and vascular smooth muscle cells. PDGF-D signaling contributes to angiogenesis, tissue remodeling, and fibrosis. Elevated PDGF-D expression promotes tumor growth, epithelial-mesenchymal transition, and metastasis in several cancers, making it a target of interest in cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PDGFD shRNA Lentivirus delivers a validated short hairpin RNA targeting human PDGFD for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of PDGF-D signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting PDGFD (NCBI Accession: NM_025208) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623879021,"sku":"LSV-0021-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311256559981,"sku":"LSV-0021-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311256592749,"sku":"LSV-0021-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311256625517,"sku":"LSV-0021-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311256658285,"sku":"LSV-0021-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311256691053,"sku":"LSV-0021-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311256723821,"sku":"LSV-0021-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311256756589,"sku":"LSV-0021-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311256789357,"sku":"LSV-0021-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311256822125,"sku":"LSV-0021-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0021-h-PDGFD-sh-fig1.jpg?v=1776934151"},{"product_id":"stat3-reporter-lentivirus-bhv19400003","title":"STAT3 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSTAT3 (signal transducer and activator of transcription 3) is a latent cytoplasmic transcription factor and a central effector of the JAK\/STAT signaling pathway. In response to cytokines such as IL-6 and growth factors, receptor-associated Janus kinases phosphorylate STAT3, which then dimerizes, translocates to the nucleus, and binds specific response elements to drive transcription. STAT3 target genes regulate cell proliferation, survival, angiogenesis, and immune responses. Persistent STAT3 activation is a common feature of many cancers and inflammatory conditions, where it promotes tumor growth and immune evasion. Because of this, STAT3 is an extensively studied node in cytokine signaling and oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe STAT3 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of STAT3 activity in human or mouse cells. Tandem consensus STAT3 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, Renilla luciferase, or SEAP), enabling quantitative monitoring of JAK\/STAT pathway activation in transduced cells. Constitutively expressed fluorescent reporters and antibiotic selection markers (puromycin or blasticidin) support tracking of transduced cells and establishment of stable reporter cell lines. The system is used to study cytokine signaling, screen pathway modulators, and characterize STAT3 activity in cancer and inflammation research. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the STAT3 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed)) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251624468845,"sku":"LTV-0003-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310892867949,"sku":"LTV-0003-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892900717,"sku":"LTV-0003-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310892933485,"sku":"LTV-0003-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310892966253,"sku":"LTV-0003-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Puromycin \/ 2x10^6","offer_id":53310892999021,"sku":"LTV-0003-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Blasticidin \/ 2x10^6","offer_id":53310893031789,"sku":"LTV-0003-8S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893064557,"sku":"LTV-0003-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0003-STAT3-Reporter-A549-stable-cell-line.jpg?v=1776934331"},{"product_id":"fluorescence-and-luciferase-dual-reporter-lentivirus-bhv19400181","title":"Fluorescence and Luciferase Dual Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTracking the location, abundance, and behavior of defined cell populations is fundamental to cancer and immunology research. Bioluminescence imaging with luciferase enables sensitive, longitudinal monitoring of cell number in living animals, while fluorescent proteins allow single-cell identification by microscopy and flow cytometry. Combining both modalities in the same cell provides complementary readouts: luciferase for whole-body imaging of tumor growth and metastasis, and fluorescence for ex vivo histology, FACS-based isolation, and analysis of tumor-infiltrating immune cells. Stable, division-resistant labeling is essential for accurate longitudinal preclinical studies of tumor biology and the tumor microenvironment.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Fluorescence and Luciferase Dual Reporter Lentivirus constitutively co-expresses a luminescent reporter (firefly or secreted Gaussia luciferase) and a fluorescent protein (GFP or RFP) from a single transcript, using self-cleaving peptides for independent, equimolar protein production. Expression is driven by a CMV, PGK, or EF1α promoter, and a selection marker (puromycin or blasticidin) supports stable cell line generation. Stable integration ensures persistent dual-reporter expression, enabling longitudinal bioluminescence imaging of tumor growth and metastasis in vivo and parallel fluorescence-based identification, gating, or FACS sorting of tumor cells for flow cytometry, scRNA-seq, and proteomics. Multiple vector layouts optimize for stable line establishment or primary-cell labeling. Supplied as ultra-purified, high-titer lentiviral particles suitable for in vitro and in vivo use, including transduction of primary and cryopreserved cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentivirus constitutively co-expresses a luciferase reporter and a fluorescent protein (GFP, RFP, Luc) from a strong CMV or EF1α promoter, using a self-cleaving P2A peptide to ensure equimolar, independent production of both reporters. A selection marker (Blasticidin, Puromycin) is co-encoded for stable cell line generation.\u003c\/p\u003e\n\u003cp\u003eThe stable lentiviral integration ensures persistent, division-stable reporter expression in the target cell line, enabling longitudinal bioluminescence imaging (IVIS) of tumor burden in mouse models and parallel fluorescence-based readouts for ex vivo analysis. The dual-modality design provides built-in redundancy — luciferase for high-sensitivity whole-body imaging and fluorescence for histology, flow cytometry, and FACS — within a single stably engineered cell line.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-Luc-GFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53251625812333,"sku":"LFV-0001-01","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-RFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53362028544365,"sku":"LFV-0001-02","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-GFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028577133,"sku":"LFV-0001-03","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-RFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028609901,"sku":"LFV-0001-04","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GFP-Puro-Luc \/ Puromycin \/ 3.5x10^6","offer_id":53362028642669,"sku":"LFV-0001-05","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-RFP-Puro-Luc \/ Puromycin \/ 3.5x10^6","offer_id":53362028675437,"sku":"LFV-0001-06","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GFP-BSD-Luc \/ Blasticidin \/ 3.5x10^6","offer_id":53362028708205,"sku":"LFV-0001-07","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-RFP-BSD-Luc \/ Blasticidin \/ 3.5x10^6","offer_id":53362028740973,"sku":"LFV-0001-08","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-GFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53362028773741,"sku":"LFV-0001-09","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-RFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028806509,"sku":"LFV-0001-10","price":595.0,"currency_code":"USD","in_stock":true},{"title":"PGK-GFP-P2A-Luc \/ N\/A \/ 5x10^6","offer_id":53362028839277,"sku":"LFV-0001-11","price":595.0,"currency_code":"USD","in_stock":true},{"title":"PGK-RFP-P2A-Luc \/ N\/A \/ 5x10^6","offer_id":53362028872045,"sku":"LFV-0001-12","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-P2A-GFP \/ N\/A \/ 5x10^6","offer_id":53362028904813,"sku":"LFV-0001-13","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-P2A-RFP \/ N\/A \/ 5x10^6","offer_id":53362028937581,"sku":"LFV-0001-14","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-RFP-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362028970349,"sku":"LFV-0001-16","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-GFP-Puro \/ Puromycin \/ 5x10^6","offer_id":53362029003117,"sku":"LFV-0001-15","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-RFP-Puro \/ Puromycin \/ 5x10^6","offer_id":53362029035885,"sku":"LFV-0001-18","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-GFP-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362029068653,"sku":"LFV-0001-17","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-rFLuc-RFP-BSD \/ Blasticidin \/ 2x10^6","offer_id":53362029101421,"sku":"LFV-0001-19","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-ZsG-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362029134189,"sku":"LFV-0001-20","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LucFluo-Fig1-JPG-3.jpg?v=1776934330"},{"product_id":"vegf-vpf-receptor-adenovirus-ad-vegf-r1-bhv21600135","title":"VEGF\/VPF receptor Adenovirus (Ad-VEGF-R1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-VEGF-R1 is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the VEGF-R1 gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e VEGF-R1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThree cell membrane receptor tyrosine kinases Flt-1 (also designated VEGF-R1), Flk-1 (also designated VEGF-R2 or KDR) and Flt-4 (putatively involved in the growth of endothelial cells) are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domains. Two members of this receptor class, Flt-1 and Flk-1, represent high affinity receptors for vascular endothelial growth factor (VEGF). On the basis of structural similarity to Flt-1 and Flk-1, Flt-4 may represent a third receptor for either VEGF or a VEGF-related ligand. Flt-3\/ Flk-2 and its human homolog, STK-1, are expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues.\u003c\/p\u003e\u003cp\u003eThis is an adenovirus expressing human sFlt1.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying VEGF Family.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1437-vegf-vpf-receptor-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286489751917,"sku":"1437","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"vascular-endothelial-growth-factor-d-adenovirus-ad-vegf-d-bhv21600116","title":"vascular endothelial growth factor D Adenovirus (Ad-VEGF-D)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-VEGF-D is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the VEGF-D gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e VEGF-D.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include FGF, PDGF, vascular endothelial growth factor (VEGF), VEGF-B, VEGF-C, VEGF-D (or FIGF), EG-VEGF and placenta growth factor (PlGF). VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying VEGF Family.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1418-vascular-endothelial-growth-factor-d-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286492275053,"sku":"1418","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"vascular-endothelial-growth-factor-b-isoform-vegf-b186-adenovirus-ad-vegf-b-bhv21600245","title":"Vascular endothelial growth factor B (isoform Vegf-b186) Adenovirus (Ad-VEGF-B)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-VEGF-B is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the VEGF-B gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e VEGF-B.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include FGF, PDGF, vascular endothelial growth factor (VEGF), VEGF-B, VEGF-C, VEGF-D (or FIGF), EG-VEGF and placenta growth factor (PlGF). This adenovirus contains a human VEGF-B186.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying VEGF Family.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1550-vascular-endothelial-growth-factor-b-isoform-vegf-b186-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286495650157,"sku":"1550","price":475.0,"currency_code":"USD","in_stock":true}]},{"product_id":"vegf-receptor-2-adenovirus-ad-vegf-r2-bhv21600309","title":"VEGF Receptor 2 Adenovirus (Ad-VEGF-R2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd-VEGF-R2 is a replication-defective recombinant human adenovirus type 5 (Ad5) expressing the VEGF-R2 gene under the CMV promoter. The vector backbone has E1 and E3 deleted, rendering it non-replicative and accommodating the transgene cassette.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e VEGF-R2.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThree cell membrane receptor tyrosine kinases Flt-1 (also designated VEGF-R1), Flk-1 (also designated VEGF-R2 or KDR) and Flt-4 (putatively involved in the growth of endothelial cells) are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domains. Two members of this receptor class, Flt-1 and Flk-1, represent high affinity receptors for vascular endothelial growth factor (VEGF). On the basis of structural similarity to Flt-1 and Flk-1, Flt-4 may represent a third receptor for either VEGF or a VEGF-related ligand. Flt-3\/ Flk-2 and its human homolog, STK-1, are expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUsed in oncology research to model gain-of-function or loss-of-function alterations in tumor-relevant pathways.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery enables high-efficiency transduction of cancer cell lines and primary tumor cells.\u003c\/li\u003e\n\u003cli\u003eDecision-relevant for researchers studying VEGF Family.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePathway activation studies in cell lines and primary cells.\u003c\/li\u003e\n\u003cli\u003eGain-of-function phenotyping in disease-relevant cell models.\u003c\/li\u003e\n\u003cli\u003eRescue experiments paired with shRNA knockdown of the same target.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1621-vegf-receptor-2-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286498009453,"sku":"1621","price":690.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.ebiohippo.com\/collections\/rtc-cancer-angiogenesis-tumor-vasculature-vectors-viruses.oembed","provider":"BioHippo","version":"1.0","type":"link"}