{"title":"Protein Quality Control — Cells","description":null,"products":[{"product_id":"adrm1-stable-knockout-hct116-clone-b11-cell-line-bhc10900954","title":"ADRM1 Stable Knockout HCT116 (Clone B11) Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eADRM1\u003c\/em\u003e (Adhesion Regulating Molecule 1, also known as RPN13) has been disrupted in the HCT116 human colorectal carcinoma background. The knockout was generated through CRISPR-Cas9–directed targeting of exon 4, replaced with a puromycin resistance cassette and pBluescriptII. This isogenic model supports investigation of proteasome biology, ubiquitin-mediated protein degradation, and cancer-related signaling. Cells grow as adherent, polygonal monolayers and are supplied frozen (BSL-II). An isogenic control cell line is available separately (Cat. No. T8240).\u003c\/p\u003e\n\n\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eADRM1\u003c\/em\u003e (Adhesion Regulating Molecule 1; also ADRM1\/RPN13)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–directed knock-in targeting exon 4; exon 4 was replaced with a puromycin resistance cassette and pBluescriptII backbone to enable selection of successfully transformed cells\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSelection marker:\u003c\/strong\u003e Puromycin resistance; 0.4 µg\/ml puromycin used for selection (add only after first passage post-thaw)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HCT116 — human colorectal carcinoma; adherent, polygonal; derived from a 48-year-old Caucasian male donor\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIsogenic control:\u003c\/strong\u003e Available separately as Cat. No. T8240\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eGene Background\u003c\/h2\u003e\n\u003cp\u003eADRM1 (Adhesion Regulating Molecule 1), also referred to as RPN13 or ADRM1\/RPN13, is a component of the 26S proteasome lid complex and functions as a ubiquitin receptor. It recruits the deubiquitinating enzyme UCHL5 (ubiquitin carboxyl-terminal hydrolase L5\/UCH37) to the proteasome, facilitating substrate processing and ubiquitin chain editing. ADRM1 is known to undergo O-linked glycosylation, which may influence its interactions within the proteasome complex. Dysregulation of ADRM1 expression has been reported in several cancer types, including ovarian and colorectal carcinomas, where elevated expression has been associated with tumor progression. These characteristics make ADRM1 a subject of interest for cancer biology and drug target studies.\u003c\/p\u003e\n\n\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\n\u003cdl\u003e\n  \u003cdt\u003eRecommended vessels\u003c\/dt\u003e\n\u003cdd\u003ePriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422)\u003c\/dd\u003e\n  \u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose (TM500) + 10% FBS (do not heat-inactivate) + 1% Penicillin\/Streptomycin Solution (G255)\u003c\/dd\u003e\n  \u003cdt\u003eCulture conditions\u003c\/dt\u003e\n\u003cdd\u003e37.0°C, 5% CO₂\u003c\/dd\u003e\n  \u003cdt\u003eSelection\u003c\/dt\u003e\n\u003cdd\u003e0.4 µg\/ml Puromycin (G264); add only after first passage post-thaw\u003c\/dd\u003e\n  \u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, polygonal\u003c\/dd\u003e\n  \u003cdt\u003eSeeding density\u003c\/dt\u003e\n\u003cdd\u003e30,000–50,000 cells\/cm²\u003c\/dd\u003e\n  \u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n  \u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eColon (colorectal carcinoma)\u003c\/dd\u003e\n  \u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n  \u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003cp\u003eKnockout is confirmed by the presence of frameshift-inducing INDELs, verified by Sanger Sequencing.\u003c\/p\u003e\n\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProteasome biology:\u003c\/strong\u003e Investigate the contribution of ADRM1 to 26S proteasome assembly, substrate recognition, and ubiquitin chain processing in a colorectal carcinoma model.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDrug target validation:\u003c\/strong\u003e Assess the effect of proteasome inhibitors (e.g., bortezomib, carfilzomib) or ADRM1-targeted agents in ADRM1 KO vs. HCT116 parental and isogenic control (T8240).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eUbiquitin pathway analysis:\u003c\/strong\u003e Profile global ubiquitination levels and specific substrate degradation using co-immunoprecipitation and mass spectrometry approaches.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCarcinogenesis research:\u003c\/strong\u003e Study the role of ADRM1 dysregulation in colorectal cancer cell proliferation, survival, and drug sensitivity.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eTranscriptomic profiling:\u003c\/strong\u003e Identify downstream transcriptional consequences of ADRM1 loss using RNA-seq comparing KO, parental, and isogenic control lines.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eImportant Notes\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSelection drug timing:\u003c\/strong\u003e Add puromycin (0.4 µg\/ml) to culture medium only after the first passage post-thaw to allow cells to recover from freeze-thaw conditions.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIsogenic control:\u003c\/strong\u003e A matched control cell line (Cat. No. T8240) is available for paired experimental designs requiring isogenic comparison.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor\/source:\u003c\/strong\u003e HCT116 parental cells are derived from a 48-year-old Caucasian male with colorectal carcinoma.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eViability warranty:\u003c\/strong\u003e Cell viability upon thaw is warranted for 30 days following shipment when handled according to abm's cell handling guidelines.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c!-- Sources (internal):\n- ADRM1 gene entry | UniProt (Q16186 human) | https:\/\/www.uniprot.org\/uniprot\/Q16186\n- ADRM1 gene record | NCBI Gene ID: 11047 | https:\/\/www.ncbi.nlm.nih.gov\/gene\/11047\n- Jiang RT et al. (2017) Early and consistent overexpression of ADRM1 in ovarian high-grade serous carcinoma. J Ovarian Res | https:\/\/doi.org\/10.1186\/s13048-017-0347-y\n- Hamazaki J et al. (2006) A Rpn10-like adaptor protein links ubiquitin and proteasome. Mol Cell | https:\/\/doi.org\/10.1016\/j.molcel.2006.06.007\n- Lam YA et al. (2002) Editing of ubiquitin conjugates by an isopeptidase in the 26S proteasome. Science | https:\/\/doi.org\/10.1126\/science.1071745\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180707963245,"sku":"T6162","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/KeKl9l4DfZRrNjKgUm2XyJ0yKduAjeeqiEXWArT9.png?v=1774960834"},{"product_id":"cul7-crispr-knockout-293t-cell-line-bhc10906669","title":"Cul7 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eCUL7\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of CUL7 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eCUL7\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eCUL7\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL7\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=CUL7+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eCUL7\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eCUL7\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by CUL7 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eCUL7\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: CUL7 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL7\n- UniProt: CUL7 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=CUL7\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181030203757,"sku":"T3004403","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/2LVGa9oiw76ObvkPU3r5FJKoyPnbE15eP15Z2tnQ.png?v=1774974295"},{"product_id":"cul1-crispr-knockout-293t-cell-line-bhc10906663","title":"CUL1 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eCUL1\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of CUL1 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eCUL1\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eCUL1\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL1\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=CUL1+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eCUL1\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eCUL1\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by CUL1 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eCUL1\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: CUL1 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL1\n- UniProt: CUL1 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=CUL1\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181030302061,"sku":"T3004397","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/58LYGWC5b51i0cSaXpNPRIkeo5Ze7bMSyCQRsnx6.png?v=1774974296"},{"product_id":"cul5-crispr-knockout-293t-cell-line-bhc10906668","title":"CUL5 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eCUL5\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of CUL5 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eCUL5\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eCUL5\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL5\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=CUL5+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eCUL5\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eCUL5\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by CUL5 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eCUL5\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: CUL5 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL5\n- UniProt: CUL5 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=CUL5\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181030695277,"sku":"T3004402","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AUNf5z07il1kBiiAxK52rzv0TojiuejcdcyxuKeF.png?v=1774974295"},{"product_id":"cul3-crispr-knockout-293t-cell-line-bhc10906665","title":"CUL3 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eCUL3\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of CUL3 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eCUL3\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eCUL3\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL3\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=CUL3+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eCUL3\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eCUL3\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by CUL3 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eCUL3\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: CUL3 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL3\n- UniProt: CUL3 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=CUL3\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181030728045,"sku":"T3004399","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/5EhnNiQGW5n28VSosn1LNV3wnNAyuRxLVbh1aA4U.png?v=1774974295"},{"product_id":"cul9-crispr-knockout-293t-cell-line-bhc10906670","title":"CUL9 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eCUL9\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of CUL9 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eCUL9\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eCUL9\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL9\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=CUL9+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eCUL9\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eCUL9\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by CUL9 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eCUL9\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: CUL9 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL9\n- UniProt: CUL9 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=CUL9\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181031743853,"sku":"T3004404","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/07DyrjoMxDpDAhq6qY9AW1HB1TvKo6yiPwW8NDSo.png?v=1774974297"},{"product_id":"cul2-crispr-knockout-293t-cell-line-bhc10906664","title":"CUL2 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eCUL2\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of CUL2 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eCUL2\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eCUL2\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL2\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=CUL2+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eCUL2\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eCUL2\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by CUL2 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eCUL2\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: CUL2 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CUL2\n- UniProt: CUL2 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=CUL2\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181031973229,"sku":"T3004398","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TPm5YJS74xsmsw4Qe0oUWDb3J5MXrRA0I6SGYcZX.png?v=1774974295"},{"product_id":"dnajb1-crispr-knockout-293t-cell-line-bhc10907353","title":"DNAJB1 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB1\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB1 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB1\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB1\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB1\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB1+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB1\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB1\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB1 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB1\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB1 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB1\n- UniProt: DNAJB1 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB1\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181052879213,"sku":"T3005087","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/6K5urIgPgbSXCppH5xsGG8qhXZctKEAWG2QYrEZ8.png?v=1774974354"},{"product_id":"dnajb3-crispr-knockout-293t-cell-line-bhc10907359","title":"DNAJB3 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB3\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB3 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB3\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB3\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB3\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB3+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB3\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB3\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB3 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB3\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB3 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB3\n- UniProt: DNAJB3 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB3\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053206893,"sku":"T3005093","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Xp5FMjd1SXLWWFQmupQKnWPX2hHwFq49Ls4xKOmC.png?v=1774974352"},{"product_id":"dnajb12-crispr-knockout-293t-cell-line-bhc10907355","title":"DNAJB12 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB12\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB12 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB12\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB12\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB12\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB12+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB12\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB12\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB12 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB12\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB12 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB12\n- UniProt: DNAJB12 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB12\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053272429,"sku":"T3005089","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1BxOyfYY7uo59mU3TJ5QmYr6SjYxMnSddKG6aOtW.png?v=1774974351"},{"product_id":"dnajb4-crispr-knockout-293t-cell-line-bhc10907360","title":"DNAJB4 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB4\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB4 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB4\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB4\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB4\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB4+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB4\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB4\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB4 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB4\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB4 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB4\n- UniProt: DNAJB4 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB4\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053403501,"sku":"T3005094","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/9qVN8Jo5IVFbb3jl6C91qpxOMtQ9yJvgvaBoLkyy.png?v=1774974353"},{"product_id":"dnajb14-crispr-knockout-293t-cell-line-bhc10907357","title":"DNAJB14 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB14\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB14 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB14\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB14\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB14\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB14+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB14\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB14\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB14 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB14\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB14 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB14\n- UniProt: DNAJB14 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB14\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053370733,"sku":"T3005091","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Xv9ApEz7igbySSDYKihHwxbeDgaxVGW0OOKxSojE.png?v=1774974351"},{"product_id":"dnajb6-crispr-knockout-293t-cell-line-bhc10907362","title":"DNAJB6 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB6\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB6 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB6\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB6\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB6\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB6+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB6\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB6\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB6 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB6\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB6 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB6\n- UniProt: DNAJB6 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB6\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053469037,"sku":"T3005096","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/xJSeXX1KmMuuDvmlIqFZHDRxWjUGOZijxkVtldCO.png?v=1774974354"},{"product_id":"dnajb13-crispr-knockout-293t-cell-line-bhc10907356","title":"DNAJB13 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB13\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB13 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB13\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB13\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB13\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB13+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB13\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB13\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB13 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB13\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB13 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB13\n- UniProt: DNAJB13 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB13\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053501805,"sku":"T3005090","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/71LUgk1msHxU36EsxgNXMYZ1kq54KJOPu4WCRWl8.png?v=1774974352"},{"product_id":"dnajb11-crispr-knockout-293t-cell-line-bhc10907354","title":"DNAJB11 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB11\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB11 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB11\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB11\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB11\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB11+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB11\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB11\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB11 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB11\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB11 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB11\n- UniProt: DNAJB11 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB11\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053796717,"sku":"T3005088","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ZtIwHox4zy28J4JLTI5FciuKf2aoqxjBC7XPeNPL.png?v=1774974355"},{"product_id":"dnajb8-crispr-knockout-293t-cell-line-bhc10907364","title":"DNAJB8 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB8\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB8 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB8\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB8\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB8\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB8+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB8\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB8\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB8 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB8\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB8 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB8\n- UniProt: DNAJB8 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB8\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053862253,"sku":"T3005098","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/OrrvGolvSbO6ZyaGP0vZmzbLDw1BkG8RilIhQiyY.png?v=1774974353"},{"product_id":"dnajb9-crispr-knockout-293t-cell-line-bhc10907365","title":"DNAJB9 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB9\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB9 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB9\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB9\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB9\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB9+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB9\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB9\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB9 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB9\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB9 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB9\n- UniProt: DNAJB9 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB9\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053895021,"sku":"T3005099","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/kiLKJEdWgCMGDk6WnDMdcG7knTI8J5DEudVr1igh.png?v=1774974353"},{"product_id":"dnajb7-crispr-knockout-293t-cell-line-bhc10907363","title":"DNAJB7 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB7\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB7 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB7\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB7\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB7\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB7+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB7\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB7\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB7 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB7\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB7 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB7\n- UniProt: DNAJB7 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB7\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181053993325,"sku":"T3005097","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bH2dL51xwmSsYnjuUL6OjGxpO3Be42tWSyWSOF2I.png?v=1774974352"},{"product_id":"dnajb5-crispr-knockout-293t-cell-line-bhc10907361","title":"DNAJB5 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB5\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB5 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB5\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB5\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB5\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB5+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB5\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB5\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB5 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB5\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB5 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB5\n- UniProt: DNAJB5 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB5\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181054189933,"sku":"T3005095","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/W9pEIJoiq73JpV03HoWUTB9QqOLqHRVGyOe0GPT1.png?v=1774974352"},{"product_id":"dnajb2-crispr-knockout-293t-cell-line-bhc10907358","title":"DNAJB2 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDNAJB2\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DNAJB2 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDNAJB2\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDNAJB2\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB2\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB2+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDNAJB2\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDNAJB2\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DNAJB2 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDNAJB2\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DNAJB2 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB2\n- UniProt: DNAJB2 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DNAJB2\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181054681453,"sku":"T3005092","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/zfmZELor6VeLSvI2OmllQ7WC8wPnU388LaP5QRln.png?v=1774974354"},{"product_id":"dusp11-crispr-knockout-293t-cell-line-bhc10907557","title":"DUSP11 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP11\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP11 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP11\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP11\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP11\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP11+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP11\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP11\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP11 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP11\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP11 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP11\n- UniProt: DUSP11 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP11\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181059989869,"sku":"T3005291","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/p0w1fRtY3kNipyZ9OyQUTgWy9CQetfjgzUSDsCb5.png?v=1774974368"},{"product_id":"dusp13-crispr-knockout-293t-cell-line-bhc10907559","title":"DUSP13 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP13\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP13 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP13\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP13\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP13\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP13+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP13\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP13\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP13 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP13\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP13 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP13\n- UniProt: DUSP13 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP13\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060186477,"sku":"T3005293","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/9a1zrugmAwkTEpd7Yyye5xir5YW3Dn9m1UflWGW2.png?v=1774974370"},{"product_id":"dusp14-crispr-knockout-293t-cell-line-bhc10907560","title":"DUSP14 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP14\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP14 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP14\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP14\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP14\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP14+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP14\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP14\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP14 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP14\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP14 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP14\n- UniProt: DUSP14 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP14\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060219245,"sku":"T3005294","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pyemcHT6x6k42KsONlnHOydxk13JHsFmJUdCxU30.png?v=1774974369"},{"product_id":"dusp22-crispr-knockout-293t-cell-line-bhc10907567","title":"DUSP22 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP22\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP22 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP22\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP22\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP22\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP22+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP22\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP22\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP22 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP22\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP22 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP22\n- UniProt: DUSP22 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP22\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060284781,"sku":"T3005301","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/UaPtxUg8CvJKwoG73uEMjZX0wIXlzfwu6dV4FyuP.png?v=1774974369"},{"product_id":"dusp21-crispr-knockout-293t-cell-line-bhc10907566","title":"DUSP21 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP21\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP21 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP21\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP21\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP21\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP21+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP21\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP21\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP21 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP21\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP21 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP21\n- UniProt: DUSP21 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP21\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060317549,"sku":"T3005300","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/dV5unT5sVw6kCuMIeQceQQRn0iGPa54jjeHsQThb.png?v=1774974369"},{"product_id":"dusp19-crispr-knockout-293t-cell-line-bhc10907564","title":"DUSP19 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP19\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP19 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP19\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP19\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP19\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP19+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP19\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP19\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP19 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP19\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP19 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP19\n- UniProt: DUSP19 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP19\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060350317,"sku":"T3005298","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/0nnOnIL9KmCG8RXPniU1YnVrCmwJYRIPH7cJxLzA.png?v=1774974369"},{"product_id":"dusp3-crispr-knockout-293t-cell-line-bhc10907572","title":"DUSP3 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP3\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP3 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP3\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP3\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP3\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP3+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP3\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP3\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP3 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP3\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP3 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP3\n- UniProt: DUSP3 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP3\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060383085,"sku":"T3005306","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/UHHJBbW08p1rrq3Vz9YlzJvbIdI3qCixgPNRXiWb.png?v=1774974372"},{"product_id":"dusp18-crispr-knockout-293t-cell-line-bhc10907563","title":"DUSP18 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP18\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP18 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP18\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP18\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP18\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP18+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP18\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP18\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP18 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP18\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP18 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP18\n- UniProt: DUSP18 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP18\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060415853,"sku":"T3005297","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/XWYL78FGVDrdRxvfwG0cveUZYQfDiUWsnVlEKDbP.png?v=1774974369"},{"product_id":"dusp5-crispr-knockout-293t-cell-line-bhc10907574","title":"DUSP5 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP5\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP5 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP5\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP5\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP5\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP5+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP5\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP5\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP5 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP5\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP5 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP5\n- UniProt: DUSP5 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP5\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060448621,"sku":"T3005308","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AUWN30GzQ49RtIfWqQY3NzpTbOgq54ihu1IAkMAs.png?v=1774974369"},{"product_id":"dusp16-crispr-knockout-293t-cell-line-bhc10907562","title":"DUSP16 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP16\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP16 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP16\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP16\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP16\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP16+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP16\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP16\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP16 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP16\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP16 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP16\n- UniProt: DUSP16 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP16\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060481389,"sku":"T3005296","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/U8FN7I48BJ0zRW0R1aqiKEDehfZjtzdrwe1Fs6T7.png?v=1774974368"},{"product_id":"dusp1-crispr-knockout-293t-cell-line-bhc10907555","title":"DUSP1 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP1\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP1 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP1\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP1\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP1\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP1+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP1\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP1\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP1 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP1\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP1 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP1\n- UniProt: DUSP1 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP1\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060514157,"sku":"T3005289","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IiXPoYeeZv3LQFSYYVbFsg1W5v4nqvw2uEx1Gnbu.png?v=1774974368"},{"product_id":"dusp26-crispr-knockout-293t-cell-line-bhc10907569","title":"DUSP26 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP26\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP26 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP26\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP26\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP26\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP26+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP26\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP26\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP26 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP26\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP26 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP26\n- UniProt: DUSP26 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP26\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060677997,"sku":"T3005303","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SCbysgjUhKYorNskWyqGlsbafzw3J1JXFRQyZM8e.png?v=1774974369"},{"product_id":"dusp6-crispr-knockout-293t-cell-line-bhc10907575","title":"DUSP6 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP6\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP6 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP6\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP6\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP6\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP6+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP6\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP6\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP6 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP6\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP6 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP6\n- UniProt: DUSP6 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP6\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060579693,"sku":"T3005309","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/L37fGugfCTBaHV5SNFgUeAMMPFz2Phyoc9c9Tmdi.png?v=1774974370"},{"product_id":"dusp10-crispr-knockout-293t-cell-line-bhc10907556","title":"DUSP10 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP10, -2\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP10, -2 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP10, -2\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP10, -2\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP10,%20-2\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP10,%20-2+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP10, -2\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP10, -2\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP10, -2 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP10, -2\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP10, -2 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP10, -2\n- UniProt: DUSP10, -2 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP10, -2\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060710765,"sku":"T3005290","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/qFmjQHY1HPW1q6Tz7BFCAzbJfG8TleJ3YUsFrGKe.png?v=1774974368"},{"product_id":"dusp28-crispr-knockout-293t-cell-line-bhc10907571","title":"DUSP28 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP28\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP28 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP28\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP28\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP28\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP28+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP28\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP28\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP28 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP28\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP28 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP28\n- UniProt: DUSP28 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP28\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060645229,"sku":"T3005305","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Xk1igkmp0IJr5bCWVZyWPrxEy7aRLuptREdAiUKz.png?v=1774974369"},{"product_id":"dusp8-crispr-knockout-293t-cell-line-bhc10907577","title":"DUSP8 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP8\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP8 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP8\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP8\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP8\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP8+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP8\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP8\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP8 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP8\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP8 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP8\n- UniProt: DUSP8 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP8\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181060841837,"sku":"T3005311","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/w6xBn6NyV7pBhjSzYsuLt5F1LxQRn1YVZMTTyrBK.png?v=1774974370"},{"product_id":"dusp15-crispr-knockout-293t-cell-line-bhc10907561","title":"DUSP15 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP15\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP15 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP15\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP15\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP15\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP15+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP15\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP15\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP15 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP15\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP15 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP15\n- UniProt: DUSP15 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP15\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061005677,"sku":"T3005295","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/T4KJBTV0CY5UzXfItyE9SGd214cFcmYVpgoeDiDk.png?v=1774974368"},{"product_id":"dusp27-crispr-knockout-293t-cell-line-bhc10907570","title":"DUSP27 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP27\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP27 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP27\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP27\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP27\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP27+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP27\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP27\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP27 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP27\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP27 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP27\n- UniProt: DUSP27 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP27\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061071213,"sku":"T3005304","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/zzoJfG1i15i01BNoKyJJuOJ11MhzFMsj5AxyYfvw.png?v=1774974369"},{"product_id":"dusp9-crispr-knockout-293t-cell-line-bhc10907578","title":"DUSP9 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP9\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP9 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP9\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP9\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP9\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP9+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP9\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP9\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP9 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP9\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP9 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP9\n- UniProt: DUSP9 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP9\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061103981,"sku":"T3005312","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/v04OpxuXDztKuoKVToa2CCam2JZ8xB5q97hWsnRO.png?v=1774974369"},{"product_id":"dusp23-crispr-knockout-293t-cell-line-bhc10907568","title":"DUSP23 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP23\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP23 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP23\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP23\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP23\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP23+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP23\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP23\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP23 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP23\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP23 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP23\n- UniProt: DUSP23 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP23\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061202285,"sku":"T3005302","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mZewGKFIOfUBOxWBzeXIosLOd3BcsIWMW9oa7IAx.png?v=1774974369"},{"product_id":"dusp4-crispr-knockout-293t-cell-line-bhc10907573","title":"DUSP4 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP4\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP4 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP4\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP4\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP4\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP4+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP4\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP4\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP4 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP4\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP4 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP4\n- UniProt: DUSP4 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP4\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061235053,"sku":"T3005307","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/6GG5W0ftpuZAtFkclSdJro2cKt2gaRgLmUXWQ7Bv.png?v=1774974369"},{"product_id":"dusp2-crispr-knockout-293t-cell-line-bhc10907565","title":"DUSP2 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP2\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP2 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP2\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP2\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP2\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP2+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP2\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP2\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP2 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP2\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP2 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP2\n- UniProt: DUSP2 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP2\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061398893,"sku":"T3005299","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/iAog5ZgIN8vJw0CboRFnM6Ek9PulOUysCh5sQiUX.png?v=1774974368"},{"product_id":"dusp7-crispr-knockout-293t-cell-line-bhc10907576","title":"DUSP7 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP7\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP7 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP7\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP7\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP7\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP7+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP7\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP7\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP7 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP7\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP7 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP7\n- UniProt: DUSP7 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP7\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061661037,"sku":"T3005310","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YtJlLPKl5ZwnTEHtFKTV2HIZt6udvNwppLsKVptw.png?v=1774974370"},{"product_id":"dusp12-crispr-knockout-293t-cell-line-bhc10907558","title":"DUSP12 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eDUSP12\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of DUSP12 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eDUSP12\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eDUSP12\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP12\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=DUSP12+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eDUSP12\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eDUSP12\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by DUSP12 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eDUSP12\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: DUSP12 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUSP12\n- UniProt: DUSP12 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=DUSP12\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53181061726573,"sku":"T3005292","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/4Al7PCxhmtClamdnojjZLLwLWxJIB7XLVhPvUThm.png?v=1774974374"},{"product_id":"fbxw4-crispr-knockout-293t-cell-line-bhc10908699","title":"FBXW4 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eFBXW4\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of FBXW4 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eFBXW4\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eFBXW4\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW4\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=FBXW4+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eFBXW4\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eFBXW4\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by FBXW4 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eFBXW4\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: FBXW4 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW4\n- UniProt: FBXW4 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=FBXW4\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53186371256685,"sku":"T3006433","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hAOCUyqh2NLPftZNVncw3dLWg7dxuFcSgFUxoBIE.png?v=1775109222"},{"product_id":"fbxw7-crispr-knockout-293t-cell-line-bhc10908701","title":"FBXW7 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eFBXW7\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of FBXW7 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eFBXW7\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eFBXW7\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW7\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=FBXW7+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eFBXW7\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eFBXW7\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by FBXW7 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eFBXW7\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: FBXW7 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW7\n- UniProt: FBXW7 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=FBXW7\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53186371420525,"sku":"T3006435","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YVjQMxUKofzeIwnHkATkaYHN0yzyvZI9EWvJVMyM.png?v=1775109221"},{"product_id":"fbxw9-crispr-knockout-293t-cell-line-bhc10908703","title":"FBXW9 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eFBXW9\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of FBXW9 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eFBXW9\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eFBXW9\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW9\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=FBXW9+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eFBXW9\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eFBXW9\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by FBXW9 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eFBXW9\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: FBXW9 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW9\n- UniProt: FBXW9 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=FBXW9\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53186371518829,"sku":"T3006437","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/G1rkLOdX2niAlGZfRgZuxjQNaB9HB9bqVpvBgst8.png?v=1775109224"},{"product_id":"fbxw5-crispr-knockout-293t-cell-line-bhc10908700","title":"FBXW5 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eFBXW5\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of FBXW5 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eFBXW5\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eFBXW5\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW5\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=FBXW5+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eFBXW5\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eFBXW5\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by FBXW5 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eFBXW5\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: FBXW5 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW5\n- UniProt: FBXW5 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=FBXW5\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53186371617133,"sku":"T3006434","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/iSpeENfi2Y6FhYBKrQbepvgtCvJw7cnIOpZhS9Pd.png?v=1775109223"},{"product_id":"fbxw12-crispr-knockout-293t-cell-line-bhc10908697","title":"FBXW12 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eFBXW12\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of FBXW12 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eFBXW12\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eFBXW12\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW12\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=FBXW12+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eFBXW12\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eFBXW12\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by FBXW12 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eFBXW12\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: FBXW12 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW12\n- UniProt: FBXW12 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=FBXW12\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53186371813741,"sku":"T3006431","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YoHySNWMBIO8AEAiIxUiGj1SJNCZqWgguEbw4qrq.png?v=1775109222"},{"product_id":"fbxw2-crispr-knockout-293t-cell-line-bhc10908698","title":"FBXW2 CRISPR Knockout 293T Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eFBXW2\u003c\/em\u003e has been disrupted in the HEK293T (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of FBXW2 in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eFBXW2\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; presence of frameshift-inducing indels, confirmed by sanger sequencing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e HEK293T — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eFBXW2\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW2\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=FBXW2+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eFBXW2\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003eDMEM, High Glucose + 10% FBS + 1% Penicillin\/Streptomycin, 37°C, 5% CO₂\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Presence of frameshift-inducing INDELs, confirmed by Sanger Sequencing.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eFBXW2\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental HEK293T line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by FBXW2 using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eFBXW2\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental HEK293T cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: FBXW2 | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW2\n- UniProt: FBXW2 | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=FBXW2\n- abm product page | abmgood.com\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53186371944813,"sku":"T3006432","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HY6zjfLDECAvjdp1xGiSvjYjISxNpbyajGyX7ELX.png?v=1775109224"}],"url":"https:\/\/www.ebiohippo.com\/collections\/rtc-cell-biology-protein-quality-control-cells.oembed?page=8","provider":"BioHippo","version":"1.0","type":"link"}