{"title":"Protein Quality Control — Vectors \u0026 Viruses","description":null,"products":[{"product_id":"atf6-reporter-lentivirus-bhv19400092","title":"ATF6 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eActivating transcription factor 6 (ATF6) is an ER membrane-bound bZIP transcription factor and one of the three main sensors of the unfolded protein response. When unfolded proteins accumulate in the endoplasmic reticulum, ATF6 traffics to the Golgi, where it is cleaved to release an active nuclear fragment. This fragment binds ER stress-response elements to induce ER chaperones and folding enzymes such as GRP78, as well as transcription factors including XBP1. The ATF6 DNA-binding sequence is related to but distinct from ATF1\/CREB elements. ATF6 signaling is essential for adapting to ER stress and is implicated in cancer, metabolic disease, and protein-misfolding disorders.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ATF6 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of consensus ATF6 DNA-binding elements upstream of a minimal promoter and a reporter gene, giving a sensitive fluorescent or luminescent readout of ATF6 transcriptional activity. The element responds specifically to active ATF6 and is strongly induced by ER stress stimuli such as tunicamycin. A wide range of reporters is available, including EGFP, mCherry, BFP2, d2GFP, firefly luciferase, Gaussia luciferase, and Renilla luciferase, with selection markers including blasticidin, hygromycin, puromycin, and zeocin. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying ER stress and the unfolded protein response.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the ATF6 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615850861,"sku":"LTV-0101-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251634037101,"sku":"LTV-0101-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53251634069869,"sku":"LTV-0101-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53251634102637,"sku":"LTV-0101-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53251634135405,"sku":"LTV-0101-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53251634168173,"sku":"LTV-0101-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Puromycin \/ 5x10^6","offer_id":53251634200941,"sku":"LTV-0101-15S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP2 \/ Blasticidin \/ 5x10^6","offer_id":53251634233709,"sku":"LTV-0101-16S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-general-2_aab39aa6-5d35-4e8d-ad95-dba95b136483.jpg?v=1776934516"},{"product_id":"aare-reporter-lentivirus-bhv19400033","title":"AARE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe amino acid response element (AARE) mediates transcription during the amino acid response, a branch of the integrated stress response that adapts cells to nutrient limitation. When amino acids are scarce, uncharged tRNAs activate the kinase GCN2, which phosphorylates the translation factor eIF2α. This selectively enhances translation of the transcription factor ATF4, which binds AARE sequences to induce genes for amino acid biosynthesis, transport, and metabolic adaptation. Prolonged amino acid or glucose deprivation can also engage ER stress signaling through the AARE. This nutrient-sensing pathway is central to cellular responses to metabolic stress and is relevant to cancer metabolism and drug development.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AARE Reporter Lentivirus is a transcription factor reporter system that uses tandem repeats of the amino acid response element from the human asparagine synthase promoter, containing the nutrient-sensing response elements NSRE-1 and NSRE-2, to drive a reporter gene. This gives a sensitive fluorescent or luminescent readout of the eIF2α\/ATF4 amino acid response pathway following amino acid or glucose deprivation. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying metabolic stress and integrated stress response signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Amino acid response (eIF2a\/ATF4) pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616047469,"sku":"LTV-0037-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310902501741,"sku":"LTV-0037-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310902534509,"sku":"LTV-0037-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310902567277,"sku":"LTV-0037-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310902600045,"sku":"LTV-0037-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310902632813,"sku":"LTV-0037-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310902665581,"sku":"LTV-0037-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_234b25e7-0049-4d1e-81d7-435ed15f8fb2.jpg?v=1776934331"},{"product_id":"esre-reporter-lentivirus-bhv19400031","title":"ESRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ER stress-response element (ESRE) is a DNA sequence that mediates transcriptional induction during the unfolded protein response (UPR), the cell's adaptive program for restoring endoplasmic reticulum protein-folding capacity. ESRE-driven transcription is controlled by the bZIP transcription factors ATF6 and XBP1. Upon ER stress, ATF6 is cleaved to release an active nuclear fragment, while IRE1-mediated splicing of XBP1 mRNA produces the potent activator XBP1s. Together they induce chaperones and folding enzymes that relieve ER stress. The UPR is central to secretory cell function and is implicated in cancer, metabolic disease, and neurodegeneration, making ESRE-driven reporters valuable for studying protein homeostasis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ESRE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem ER stress-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of ATF6 and XBP1 activity in transduced cells. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring the unfolded protein response, studying the IRE1\/XBP1 and ATF6 branches of ER stress signaling, and screening modulators of protein homeostasis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the ER stress response transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616768365,"sku":"LTV-0035-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310897095021,"sku":"LTV-0035-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310897127789,"sku":"LTV-0035-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897160557,"sku":"LTV-0035-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310897193325,"sku":"LTV-0035-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310897226093,"sku":"LTV-0035-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897258861,"sku":"LTV-0035-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_ab1e4f4f-55db-4002-be75-82fbed2b325a.jpg?v=1776934330"},{"product_id":"atf4-reporter-lentivirus-bhv19400044","title":"ATF4 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eATF4 (activating transcription factor 4) is a basic leucine zipper transcription factor and a master effector of the integrated stress response. In response to amino acid deprivation, endoplasmic reticulum stress, oxidative stress, and other insults, stress-sensing kinases phosphorylate the translation initiation factor eIF2-alpha, which selectively promotes ATF4 translation. ATF4 then binds C\/EBP-ATF response elements (CARE) and induces genes for amino acid transport and synthesis, including aminoacyl-tRNA synthetases, as well as genes governing redox balance, autophagy, and cell fate. Through these activities ATF4 coordinates adaptation to stress and is implicated in cancer metabolism, neurodegeneration, and metabolic disease.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ATF4 Reporter Lentivirus places a reporter gene under the control of tandem C\/EBP-ATF response elements (CARE), providing a sensitive readout of ATF4 transcriptional activity and the amino acid stress response in human and mouse cells. Reporter options include fluorescent (GFP, RFP, EGFP, mCherry) and luminescent (firefly and Renilla luciferase) formats, with puromycin or blasticidin selection for stable cell line establishment. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring the integrated stress response and screening compounds that modulate ATF4 signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the ATF4\/Amino acid response transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617456493,"sku":"LTV-0048-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310904369517,"sku":"LTV-0048-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310904402285,"sku":"LTV-0048-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310904435053,"sku":"LTV-0048-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310904467821,"sku":"LTV-0048-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310904500589,"sku":"LTV-0048-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310904533357,"sku":"LTV-0048-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_23cf581a-568f-444f-ba6f-408765ce39cb.jpg?v=1776934148"},{"product_id":"clear-reporter-lentivirus-bhv19400053","title":"CLEAR Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CLEAR (Coordinated Lysosomal Enhancement and Regulation) gene network is a transcriptional program that governs lysosomal biogenesis, autophagy, and vesicular trafficking. Its master regulator is TFEB (transcription factor EB), a basic helix-loop-helix leucine-zipper transcription factor that binds CLEAR elements in the promoters of genes encoding lysosomal enzymes, lysosomal structural proteins, and core autophagy machinery. TFEB activity is controlled by the mTORC1-AKT axis: under nutrient-rich conditions mTORC1 phosphorylates TFEB and retains it in the cytoplasm, whereas starvation, lysosomal stress, or mTOR inhibition trigger TFEB dephosphorylation and nuclear translocation. Dysregulated TFEB signaling is implicated in lysosomal storage disorders, neurodegeneration, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CLEAR Reporter Lentivirus is a lentiviral transcription-factor reporter system that gives a sensitive fluorescent (GFP or RFP) or luminescent (firefly luciferase) readout of CLEAR gene network activity, reflecting TFEB transcriptional output. Tandem repeats of modified CLEAR elements drive reporter expression upon TFEB activation, enabling detection of autophagy-lysosome pathway induction by stressors, nutrient deprivation, and mTOR-AKT inhibition. The particles are purified by PEG precipitation and sucrose gradient centrifugation, and a constitutively expressed drug-selection marker (puromycin or blasticidin) supports rapid establishment of stable reporter cell lines. The system efficiently transduces difficult-to-transfect cells, including primary and cryopreserved cultures, and supports readout by fluorescence microscopy, flow cytometry, or luminometry for autophagy and protein-homeostasis studies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the CLEAR gene network transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251618963821,"sku":"LTV-0057-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893621613,"sku":"LTV-0057-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310893654381,"sku":"LTV-0057-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893687149,"sku":"LTV-0057-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310893719917,"sku":"LTV-0057-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310893752685,"sku":"LTV-0057-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893785453,"sku":"LTV-0057-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0057-CLEAR-Reporter-fig1.jpg?v=1776934147"},{"product_id":"hse-reporter-lentivirus-bhv19400008","title":"HSE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe heat shock element (HSE) is a conserved promoter motif that mediates the heat shock response, the cell's protective program against proteotoxic stress. Heat, oxidative stress, and protein-damaging conditions activate heat shock factor 1 (HSF1), which trimerizes, accumulates in the nucleus, and binds HSEs to induce molecular chaperones such as HSP70 and HSP90. These chaperones refold or clear misfolded proteins and restore proteostasis. HSF1 also influences cell survival, metabolism, and stress adaptation, and its activity is frequently elevated in cancer to support malignant proteostasis. HSE-driven reporters provide a direct readout of HSF1 activation and heat shock response signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HSE Reporter Lentivirus places a reporter gene under the control of tandem heat shock elements, providing a sensitive readout of HSF1 activation and heat shock response pathway activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with puromycin or blasticidin selection for rapid generation of stable reporter cell lines. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring proteotoxic stress responses, studying proteostasis in cancer, and screening compounds that modulate HSF1 activity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Heat shock response pathway (HSF-1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251619062125,"sku":"LTV-0009-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310891884909,"sku":"LTV-0009-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310891917677,"sku":"LTV-0009-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891950445,"sku":"LTV-0009-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310891983213,"sku":"LTV-0009-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892015981,"sku":"LTV-0009-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_2c5d26ae-5462-4d2e-a1cc-4a37a55815fd.jpg?v=1776934271"},{"product_id":"upre-reporter-lentivirus-bhv19400032","title":"UPRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe unfolded protein response (UPR) is an adaptive signaling network that maintains protein-folding homeostasis in the endoplasmic reticulum. When misfolded proteins accumulate, three ER sensors are activated: IRE1 (ERN1), which splices XBP1 mRNA to generate the active transcription factor sXBP1; ATF6, which is cleaved to release a transcriptionally active fragment; and PERK. The unfolded protein response element (UPRE) is a promoter motif bound by sXBP1 and ATF6 to induce genes encoding chaperones and components of ER-associated degradation. Sustained or unresolved ER stress contributes to metabolic disease, neurodegeneration, and cancer, making UPRE-dependent transcription a key readout of cellular stress signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe UPRE Reporter Lentivirus is a transcription-factor reporter system that detects unfolded protein response activity in human and mouse cells. The construct uses tandem repeats of the UPR element, containing DNA-binding motifs for sXBP1 and ATF6, to drive a fluorescent or luminescent reporter (firefly luciferase, Renilla luciferase, GFP, or RFP). A constitutively expressed selection marker supports stable polyclonal cell line generation, with readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe system is suited to monitoring ER stress, studying IRE1-XBP1 and ATF6 signaling, and screening compounds that modulate the UPR. Particles are purified by PEG precipitation and sucrose gradient centrifugation, enabling effective transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Unfolded protein response (UPR) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625877869,"sku":"LTV-0036-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896144749,"sku":"LTV-0036-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896177517,"sku":"LTV-0036-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896210285,"sku":"LTV-0036-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896243053,"sku":"LTV-0036-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896275821,"sku":"LTV-0036-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896308589,"sku":"LTV-0036-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_0c83e104-b89b-4f37-8b60-4ca6b58449ac.jpg?v=1776934147"}],"url":"https:\/\/www.ebiohippo.com\/collections\/rtc-cell-biology-protein-quality-control-vectors-viruses.oembed","provider":"BioHippo","version":"1.0","type":"link"}