{"title":"SignaGen Transfection Reagents","description":"\u003cp\u003eDNA and siRNA transfection reagents from SignaGen Laboratories, including cell-type pre-optimized GenJet™ and GenMute™ formulations, in vivo reagents, and matching buffers. Use the filters to narrow by nucleic acid type and application.\u003c\/p\u003e","products":[{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-3ll-cells-bht2200005","title":"GenJet™ In Vitro DNA Transfection Reagent for 3LL Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e3LL cells grew out of a spontaneous lung carcinoma that appeared in a C57BL\/6 mouse, and Dr. Margaret R. Lewis at the Wistar Institute gave the first description of it. Ever since, the line has served as a syngeneic murine tumor model, and it remains in broad use for lung cancer biology, tumor metastasis, immuno-oncology, and therapeutic development. For laboratories working with it, GenJet™ DNA In Vitro Transfection Reagent for 3LL Cells arrives with conditions already tuned to push DNA into 3LL (Lewis Lung Carcinoma) cells at high efficiency.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003e3LL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under the recommended conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-LLC.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for 3LL Cells – GFP transfection in 3LL cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eRepresentative Image Showing the Transfection Efficiency of GenJet™ Reagent in 3LL Cells. 3LL (Lewis Lung Carcinoma) cells were cultured according to ATCC-recommended conditions and transfected with the pEGFP-N3 expression plasmid using GenJet™ reagent. Transfection efficiency was evaluated 24 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898792813,"sku":"SL100489-3LL-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902102381,"sku":"SL100489-3LL-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902135149,"sku":"SL100489-3LL-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902167917,"sku":"SL100489-3LL-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-LLC_5b344c49-8fa2-463f-8d9a-045363b8e1b6.jpg?v=1790175948"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-bhk-21-cells-bht2200008","title":"GenJet™ In Vitro DNA Transfection Reagent for BHK-21 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eKidneys of newborn Syrian hamsters supplied the origin of BHK-21 (Baby Hamster Kidney-21), a continuous, fibroblast-like line that virology, recombinant protein expression, and vaccine development all draw on heavily. Vigorous growth paired with ready susceptibility to viral infection has carried BHK-21 to the front rank of mammalian cell lines used in producing viral vaccines and biologics, and where bioprocessing runs at large scale, suspension-adapted derivatives of the line are the usual choice. GenJet™ DNA In Vitro Transfection Reagent for BHK-21 Cells comes with conditions tuned to that line, so plasmid DNA transfection in BHK-21 cells proceeds efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eBHK-21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898760045,"sku":"SL100489-BHK-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902298989,"sku":"SL100489-BHK-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902331757,"sku":"SL100489-BHK-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902364525,"sku":"SL100489-BHK-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"calfectin-mammalian-cell-transfection-reagent-bht2200002","title":"CalFectin™ Mammalian Cell Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eCalFectin™ Mammalian DNA Transfection Reagent is a refined, pre-optimized calcium phosphate–based transfection reagent built on a novel chemistry. It carries a significantly lower calcium concentration than traditional calcium phosphate methods, which markedly reduces cytotoxicity while transfection efficiency is not compromised. The reagent works highly effectively for both transient and long-term stable transfection across most adherent mammalian cell lines.\u003c\/p\u003e\n\u003cp\u003eAccording to SignaGen, CalFectin™ outperforms liposome- and polymer-based transfection reagents, with superior performance and higher efficiency in a wide range of cell types, among them HepG2, HEK293, CHO, HeLa, COS, NIH\/3T3, LNCaP, C6, PC12, and primary cultured cells. A single 1.0 mL vial of CalFectin™ Reagent covers roughly 666 transfections in 24-well plates or 333 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh efficiency: components optimized for high transfection efficiencies\u003c\/li\u003e\n\u003cli\u003eSimple procedure: complete reaction in one tube\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-CalFectvsL2K-1.jpg?v=1790173423\" alt=\"CalFectin™ Mammalian Cell Transfection Reagent – GFP-tagged 14 kb DNA transfection in HEK293T cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison study showing transfection efficiency of CalFectin™ Reagent vs. a leading product, Lipofectamine 2000 on HEK293T cells. HEK293T cells were transfected with GFP-tagged DNA (total 14 kb) by CalFectin™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-CalFectvsHD-3.jpg?v=1790173423\" alt=\"CalFectin™ Mammalian Cell Transfection Reagent – GFP transfection in HepG2 cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison study showing the transfection efficiency of CalFectin™ Reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by CalFectin™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eStable or transient transfection of eukaryotic cells at high efficiency\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898858349,"sku":"SL100478-0P1ML","price":20.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901315949,"sku":"SL100478-0P5ML","price":68.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901348717,"sku":"SL100478-1P0ML","price":118.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901381485,"sku":"SL100478-1P0MLX5","price":560.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-CalFectin-vs-L2K_jpg_6e01a95f-0d8c-40a2-984e-757609d4e24b.webp?v=1790175945"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-cho-cells-bht2200011","title":"GenJet™ In Vitro DNA Transfection Reagent for CHO Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eChinese Hamster Ovary (CHO) cells sit among the most heavily used mammalian lines for recombinant protein expression, antibody production, and biopharmaceutical manufacturing — a position resting on robust growth characteristics together with the capacity for complex post-translational modifications. GenJet™ DNA In Vitro Transfection Reagent for CHO Cells reaches the bench with conditions already tuned, so DNA enters CHO cells at high efficiency.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under the recommended conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-CHO.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for CHO Cells – GFP transfection in CHO cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative Image Showing the Transfection Efficiency of GenJet™ Reagent in CHO Cells. CHO cells were cultured according to ATCC-recommended conditions and transfected with the pEGFP-N3 expression plasmid using GenJet™ reagent. Transfection efficiency was evaluated 24 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898891117,"sku":"SL100489-CHO-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902593901,"sku":"SL100489-CHO-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902626669,"sku":"SL100489-CHO-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902659437,"sku":"SL100489-CHO-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-CHO_bf2843d9-abdf-4677-b951-df422ee7812d.jpg?v=1790175947"},{"product_id":"powerfect-transfection-buffer-5x-bht2200059","title":"PowerFect™ Transfection Buffer (5x)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePowerFect™ Transfection Buffer was developed for use with PowerFect™ siRNA \u0026amp; DNA Transfection Reagent to give optimal transfection efficiency and maximum knockdown of gene expression. The buffer serves to dilute siRNA\/DNA and PowerFect™ Transfection Reagent so that the most efficient transfection complex forms. Supplied as a 5x concentrated stock solution, PowerFect™ Transfection Buffer (5x), 8.0 mL, makes 40 mL of working solution.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eBuffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at RT. If stored properly, the product is stable for 24 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003ePowerFect™ Transfection Buffer, 8.0 mL\/vial, to make 40 mL of working solution (1x ), sufficient for 1000 transfections in 24-well plate\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis transfection buffer is used to prepare transfection complexes with the matching SignaGen transfection reagent, for both DNA and siRNA delivery. Use it to dilute the nucleic acid and the reagent as described in the protocol.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"1.0 mL","offer_id":54042898923885,"sku":"SL100574-1P0ML","price":5.0,"currency_code":"USD","in_stock":true},{"title":"4.0 mL","offer_id":54042906919277,"sku":"SL100574-4P0ML","price":20.0,"currency_code":"USD","in_stock":true},{"title":"8.0 mL","offer_id":54042906952045,"sku":"SL100574-8P0ML","price":39.0,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-keratinocytes-bht2200020","title":"GenJet™ In Vitro DNA Transfection Reagent for Keratinocytes","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eKeratinocytes are difficult targets: nucleic acid uptake is limited, and the cells are sensitive to transfection-induced cytotoxicity. Proprietary formulation and optimization let GenJet™ reagent work around both obstacles, reaching high transfection efficiency with excellent cell viability kept intact, and as many as 50% GFP-positive cells under optimized conditions. GenJet™ DNA In Vitro Transfection Reagent for Keratinocytes is tuned in advance for primary and immortalized keratinocytes alike, whether human or mouse.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eKeratinocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 600 transfections in 24-well plates or 300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898956653,"sku":"SL100489-KERA-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902200685,"sku":"SL100489-KERA-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902233453,"sku":"SL100489-KERA-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902266221,"sku":"SL100489-KERA-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-hela-cells-bht2200016","title":"GenJet™ In Vitro DNA Transfection Reagent for HeLa Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eTaken from a human cervical adenocarcinoma, HeLa cells (ATCC® CCL-2.2™) rank among the most heavily used lines in biomedical research, a standing that follows from robust growth together with high transfectability. GenJet™ DNA In Vitro Transfection Reagent for HeLa Cells arrives with conditions already tuned, so DNA reaches HeLa cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHeLa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 600–1200 transfections in 24-well plates or 300–600 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-Hela.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HeLa Cells – GFP transfection in HeLa cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative fluorescence microscopy image demonstrating the transfection efficiency of GenJet™ Reagent in HeLa cells (ATCC® CCL-2.2™). Cells were cultured under ATCC-recommended growth conditions and transfected with the pEGFP-N3 reporter plasmid using GenJet™ Reagent. Transgene expression was assessed 48 hours post-transfection by monitoring GFP fluorescence, which served as an indicator of DNA delivery and gene expression efficiency.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898989421,"sku":"SL100489-HELA-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902004077,"sku":"SL100489-HELA-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902036845,"sku":"SL100489-HELA-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902069613,"sku":"SL100489-HELA-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-Hela_52caa854-f60e-4191-bf65-290af5323697.jpg?v=1790175946"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-l929-cells-bht2200021","title":"GenJet™ In Vitro DNA Transfection Reagent for L929 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eSubcutaneous connective tissue was the original source of L929, a mouse fibroblast-like line that laboratories commonly use for studies of cytotoxicity, apoptosis, inflammation, and cytokine signaling. Its marked sensitivity to cell death driven by tumor necrosis factor-alpha (TNF-α) also makes it a standard tool in TNF bioassays and related research applications. GenJet™ DNA In Vitro Transfection Reagent for L929 Cells comes tuned for efficient DNA transfection of L929 cells.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eL929\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899022189,"sku":"SL100489-L929-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903085421,"sku":"SL100489-L929-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903118189,"sku":"SL100489-L929-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903150957,"sku":"SL100489-L929-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"lipojet-in-vitro-transfection-kit-ver-ii-bht2200001","title":"LipoJet™ In Vitro Transfection Kit (Ver. II)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNovel fluorinated cationic lipids form the basis of LipoJet™ Transfection Kit, which is built on SignaGen's innovative, proprietary lipid-conjugation technology. SignaGen presents the kit as the most powerful gene delivery tool available and at the same time a very gentle one, holding significant advantages over competing lipid transfection reagents on the market, and as the more cost-effective option next to leading products there, always giving higher transfection efficiency with less cytotoxicity. Its applications span plasmid DNA, siRNA, or the two together, across most mammalian cell types.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C for LipoJet™ Reagent and RT for LipoJet™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLipoJet™ Reagent, 1.0 ml sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate)\u003c\/li\u003e\n\u003cli\u003eLipoJet™ Transfection Buffer (5x), 8.0 mL to make 40 mL of working solution (1x )\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eVersatile: DNA transfection, siRNA transfection and combined DNA\/siRNA co-transfection\u003c\/li\u003e\n\u003cli\u003ePowerful: outstanding efficiency in adherent cells\u003c\/li\u003e\n\u003cli\u003eExtremely low toxicity: better cell viability following transfection\u003c\/li\u003e\n\u003cli\u003eEconomical: more transfections per unit of reagent\u003c\/li\u003e\n\u003cli\u003eEasy to use: single-tube reaction, compatible with serum\/antibiotics\u003c\/li\u003e\n\u003cli\u003eSuited to high-throughput work\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_Reagent.jpg?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP transfection vs other reagents in HeLa, Cos-7, NIH-3T3, CHO and HEK293\" loading=\"lazy\"\u003e\u003cfigcaption\u003eAn efficiency comparison of LipoJet™ reagent vs. brand-name products to transfect HeLa, Cos-7, NIH-3T3, CHO, and HEK293 (left panel) and Cos-7 (right panel). pEGFP-N3 cDNA (0.5 µg\/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in the presence of serum (10% FBS) and antibiotics as recommended by the manufacturers. The transfection efficiency was analyzed via FACS 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_Cell_Viability.jpg?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – HeLa cell viability vs other reagents by MTT assay and phase contrast\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA toxicity comparison of LipoJet™ reagent vs. brand-name products to transfect HeLa cells. pEGFP-N3 cDNA (1.0 µg\/well of 24-well plate) was transfected to HeLa cells per the standard transfection protocols in the presence of serum (10% FBS) and antibiotics as recommended by the manufacturers. The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_L2K_PolyJet_293T.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP transfection in HEK293T cells vs Lipofectamine 2000 and PolyJet™\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg\/well, 0.25 µg\/well and 0.5 µg\/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™\/DNA (µl\/µg) ratio=2), L2K (middle panel at L2K\/DNA (µl\/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™\/DNA (µl\/µg) ratio=3) respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_L2K_PolyJet_HELA.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP transfection in HeLa cells vs Lipofectamine 2000 and PolyJet™\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HeLa cells. pEGFP-N3 cDNA (0.125 µg\/well, 0.25 µg\/well and 0.5 µg\/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™\/DNA (µl\/µg) ratio=2), L2K (middle panel at L2K\/DNA (µl\/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™\/DNA (µl\/µg) ratio=3) respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_HEK_GFP_siRNA.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP silencing by DNA\/siRNA co-transfection in HEK293 cells\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_Hela_GFP_siRNA.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP silencing by DNA\/siRNA co-transfection in HeLa cells\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_SAOS_GFP_siRNA.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP silencing by DNA\/siRNA co-transfection in SaoS-2 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExceptional gene silencing of LipoJet™ reagent during DNA\/siRNA co-transfection on mammalian cells. Transfection of GFP cDNA (left panel, 0.25 µg per well of 24-well plate) and co-transfection of GFP cDNA ( 0.25 µg per well of 24-well plate) and GFP targeted siRNA (final 10 nM, right panel) with LipoJet™ reagent leads to exceptional gene silencing on HEK293 (upper panel), Hela (middle panel) and SaoS-2 (lower panel) cells respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_HUVEC_mCherry-FITC-siRNA.jpg?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – mCherry DNA and FITC-siRNA co-transfection in HUVEC\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExceptional DNA\/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) and FITC-conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) and nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection. The pictures were given by Dr. Pan Kong of USC as a courtesy.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_HEK293_EG5_siRNA.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – KIF11 (EG5) siRNA knockdown causing cell rounding in HEK293 cells\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_Hela_EG5_siRNA.gif?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – KIF11 (EG5) siRNA knockdown causing cell rounding in HeLa cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExcellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and HeLa (lower panel) cells with LipoJet™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and HeLa cells, leading to more than 80% of “round-up” phenotype of HEK293 and HeLa cells 24h post-transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of “rounded-up” HEK293 and HeLa cells was visualized 24h post-transfection with a Nikon microscope.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_hESCs.jpg?v=1790173423\" alt=\"LipoJet™ In Vitro Transfection Kit (Ver. II) – GFP transfection in human embryonic stem cells (hESCs), DIC and fluorescence\" loading=\"lazy\"\u003e\u003cfigcaption\u003eAn image showing the exceptional transfection efficiency of LipoJet™ Transfection Kit on Human embryonic stem cells (hESCs). The hESCs grown in E8 medium on Geltrexvs (left panel, DIC imaging) were transfected with pEF1α-GFP. The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope 24 hours post-transfection. The above pictures were provided by Dr. Marina Pryzhkova of Johns Hopkins University as a courtesy\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch3\u003eTransfection Efficiency by Cell Line\u003c\/h3\u003e\n\u003ctable class=\"bhp-spec-table bhp-data-table\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCell Line\u003c\/th\u003e\n\u003cth\u003eDNA Transfection Efficiency\u003c\/th\u003e\n\u003cth\u003esiRNA Transfection Efficiency\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e293, 293T\u003c\/td\u003e\n\u003ctd\u003e85%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3T3, NIH 3T3\u003c\/td\u003e\n\u003ctd\u003e65%\u003c\/td\u003e\n\u003ctd\u003e90% at 20 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3T3-L1\u003c\/td\u003e\n\u003ctd\u003e30%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eA549\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003e90% at 10 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eB16-F10\u003c\/td\u003e\n\u003ctd\u003e75%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBNLCL.2\u003c\/td\u003e\n\u003ctd\u003e55%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC2C12\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003e90% at 10 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC3H\/10T1\/2\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCaco-2\u003c\/td\u003e\n\u003ctd\u003e20%\u003c\/td\u003e\n\u003ctd\u003e80 % at 20 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRat primary cardiomyocytes\u003c\/td\u003e\n\u003ctd\u003e10%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCOS1\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCOS7\u003c\/td\u003e\n\u003ctd\u003e75%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman bladder carcinomal cell\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emES\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eiMEF\u003c\/td\u003e\n\u003ctd\u003e55%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHBEC\u003c\/td\u003e\n\u003ctd\u003e45%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHCT116\u003c\/td\u003e\n\u003ctd\u003e75%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHela\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHepG2\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHT-29\u003c\/td\u003e\n\u003ctd\u003e45%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuH-7\u003c\/td\u003e\n\u003ctd\u003e45%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLNCaP\u003c\/td\u003e\n\u003ctd\u003e45%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMC3T3-E1\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMCF10A\u003c\/td\u003e\n\u003ctd\u003e40%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMCF7\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMDA-MB-231\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMDCK\u003c\/td\u003e\n\u003ctd\u003e20%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMEF\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e85% at 30 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman primary melanocytes\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e85% at 30 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman primary melanoma\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman immortalized microglial\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emIMCD-3\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMRC-5\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eN2A\u003c\/td\u003e\n\u003ctd\u003e68%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePC3\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRAW 264.7\u003c\/td\u003e\n\u003ctd\u003e45%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPE\u003c\/td\u003e\n\u003ctd\u003e30%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSH-SY-5Y\u003c\/td\u003e\n\u003ctd\u003e75%\u003c\/td\u003e\n\u003ctd\u003e90% at 25 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSK-OV-3\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eU-2OS\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVERO\u003c\/td\u003e\n\u003ctd\u003e40%\u003c\/td\u003e\n\u003ctd\u003e–\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ehESCs\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003ctd\u003e90% at 40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899054957,"sku":"SL100468-0P1ML","price":40.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901086573,"sku":"SL100468-0P5ML","price":208.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901119341,"sku":"SL100468-1P0ML","price":388.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901152109,"sku":"SL100468-1P0MLX5","price":1843.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-LipoJet_L2K_PolyJet_293T_1e127633-5463-48d7-9175-5f831548b0d2.gif?v=1790175945"},{"product_id":"pepmute-plus-sirna-transfection-reagent-bht2200056","title":"PepMute™ Plus siRNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eAn upgrade on PepMute™ Reagent (Cat # SL100566), PepMute™ Plus siRNA \u0026amp; DNA Transfection Reagent owes its improvement to pre-screened hydrophobic amino acids crosslinked onto the peptide backbone. Every amine group along that 36–amino-acid backbone has been modified with hydrophobic amino acids, drawing on SignaGen's proprietary pH-Dependent Conformational Change (PDCC) technology. Self-assembly while the transfection complex forms follows from this design, yielding what SignaGen calls a highly versatile and powerful gene delivery platform.\u003c\/p\u003e\n\u003cp\u003eAt 1 nM siRNA, silencing efficiency exceeds 95% across mammalian cell types of many kinds. Single DNA, single siRNA, and co-transfection of DNA together with siRNA have each been validated as efficient and reproducible in various mammalian cells.\u003c\/p\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_Plus.jpg?v=1790173498\" alt=\"PepMute™ Plus siRNA Transfection Reagent – cartoon illustrating how the reagent was developed\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 1. A cartoon showing PepMute™ Plus siRNA Transfection Reagent was developed\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C for PepMute™ Plus Reagent and RT for PepMute™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePepMute™ Plus Transfection Reagent, 1.0 mL, sufficient for ~1000 reactions based on transfecting 2.5 pmols siRNA in 24-well plate\u003c\/li\u003e\n\u003cli\u003ePepMute™ Plus Transfection Buffer(5x), formulated for maximal transfection efficiency, 8.0 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEffective knockdown (95%) using just 0.5 pmol siRNA (1.0 nM siRNA in a 24-well plate)\u003c\/li\u003e\n\u003cli\u003eFormulated for mammalian cells that are hard to transfect\u003c\/li\u003e\n\u003cli\u003eEasy, single-tube reaction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_RL.jpg?v=1790173498\" alt=\"PepMute™ Plus siRNA Transfection Reagent – Renilla luciferase siRNA knockdown in HEK293 vs Dharmafect™ 4 and L2K\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 2. Knockdown efficacy comparison of PepMute™ Plus Transfection Reagent (upper panel) vs. Dharmafect™ 4 siRNA Transfection Reagent (middle panel) and Lipofectamine™ 2000 (lower panel) on HEK293 cells. siRNA targeting renilla luciferase at different final concentrations ranging from 0.5 to 20 nM was co-transfected with the renilla luciferase gene (0.5 µg of pRL-CMV DNA per well) by the above three transfection reagents per manufacturer’s protocols into HEK293 cells growing on a 24-well plate. Renilla luciferase activity was determined 24h after post-transfection with the renilla luciferase determination system (Promega). The luminescence was measured from 5.0 µl of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity was expressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The error bars represent the standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells. While PepMute™ Plus and Dharmafect™ 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, lipofectamine™ 2000 gave good knockdown only after 30 nM (data not shown).\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_GFP.jpg?v=1790173499\" alt=\"PepMute™ Plus siRNA Transfection Reagent – GFP siRNA knockdown of co-transfected GFP in HepG2 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 3. PepMute™ Plus Transfection Reagent knocked down GFP gene expression in HepG2 cells. GFP cDNA, pEGFP-N3, was co-transfected with a siRNA targeting the GFP gene (final 5.0 nM, right panel) and a sham siRNA (final 5.0 nM, left panel) into HepG2 cells by PepMute™ Plus Transfection Reagent. GFP fluorescence was visualized 24h post-transfection with a Nikon T2000 fluorescence microscope. Quantitative analysis showed that GFP siRNA at 5.0 nM delivered by PepMute™ Plus Transfection Reagent knocked down 96% co-transfected GFP expression in HepG2 cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899087725,"sku":"SL100571-0P1ML","price":40.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905477485,"sku":"SL100571-0P5ML","price":206.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905510253,"sku":"SL100571-1P0ML","price":398.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905543021,"sku":"SL100571-1P0MLX5","price":1890.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_GFP_27ab3365-8123-412d-8a66-63e5e1f7d77a.jpg?v=1790175948"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mda-mb231-cells-bht2200023","title":"GenJet™ In Vitro DNA Transfection Reagent for MDA-MB231 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eMDA-MB-231 traces back to the pleural effusion of a 51-year-old female patient whose breast cancer had metastasized; the resulting line is classed as a human breast adenocarcinoma. Under the microscope the cells look epithelial-like and spindle-shaped, and researchers turn to them routinely as an in vitro stand-in for aggressive, highly invasive triple-negative breast cancer (TNBC). What defines the line is vigorous migration and invasion, a high metastatic potential, and growth that does not depend on anchorage.\u003c\/p\u003e\n\u003cp\u003eThose same traits make it a valuable system for probing tumor progression, epithelial–mesenchymal transition (EMT), cancer cell invasion, metastasis, and the evaluation of novel therapeutic strategies. Rapid proliferation and the line's unique biological characteristics do mean transfection conditions frequently have to be tuned so that gene delivery stays efficient and the cells stay viable. GenJet™ DNA In Vitro Transfection Reagent for MDA-MB-231 Cells ships pre-optimized for exactly that, moving plasmid DNA into MDA-MB-231 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMDA-MB231\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under the recommended conditions, GenJet™ reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300 transfections in 24-well plates or 150 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-MB231.jpg?v=1790173498\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for MDA-MB231 Cells – GFP transfection in MDA-MB-231 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection efficiency of GenJet™ Transfection Reagent in MDA-MB-231 cells. MDA-MB-231 cells were cultured under ATCC-recommended growth conditions and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was assessed 24 hours post-transfection by evaluating GFP expression using fluorescence microscopy.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899120493,"sku":"SL100489-MB231-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904822125,"sku":"SL100489-MB231-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904854893,"sku":"SL100489-MB231-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904887661,"sku":"SL100489-MB231-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-MB231_d276963c-9f82-4a8a-aa52-14463f07d3ad.jpg?v=1790175946"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-c6-glioma-cells-bht2200010","title":"GenJet™ In Vitro DNA Transfection Reagent for C6 Glioma Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eChemically induced rat glioma gave rise to the C6 line, now among the most heavily used in vitro systems in neurobiology, neuro-oncology, and glioma research. Set against primary neural cells, C6 offers clear benefits: simple culture, robust growth, and ready expansion to large cell numbers. Conventional non-viral gene delivery methods, though, often struggle with C6 cells.\u003c\/p\u003e\n\u003cp\u003eGenJet™ DNA In Vitro Transfection Reagent for C6 Glioma Cells was tuned to get past that hurdle, and under optimized conditions it moves plasmid DNA into C6 glioma cells at transfection efficiencies above 60%, while cell viabilities stay over 90%.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eC6 Glioma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899186029,"sku":"SL100489-C6-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903478637,"sku":"SL100489-C6-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903511405,"sku":"SL100489-C6-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903544173,"sku":"SL100489-C6-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-raw-264-7-cells-bht2200050","title":"GenMute™ siRNA Transfection Reagent for RAW 264.7 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eEfficient siRNA delivery into RAW 264.7 cells is the purpose GenMute™ siRNA Transfection Reagent for RAW 264.7 Cells was optimized around. The line itself is macrophage-like and murine, obtained from BALB\/c mice and transformed using Abelson murine leukemia virus. Inflammation, macrophage biology, host–pathogen interactions, and signal transduction are common subjects of study in it.\u003c\/p\u003e\n\u003cp\u003eOnce transfected, the cells still suit routine immunocytochemistry protocols aimed at protein expression and protein–protein interactions.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eRAW 264.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003e90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent (1.0 mL): Sufficient for approximately 833 transfections based on delivery of 15 pmol siRNA or miRNA mimics per well in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5×, 8.0 mL): Optimized for maximal transfection efficiency; yields 40 mL of 1× working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899153261,"sku":"SL100568-RAW-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906329453,"sku":"SL100568-RAW-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906362221,"sku":"SL100568-RAW-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906394989,"sku":"SL100568-RAW-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-neuro-2a-cells-bht2200047","title":"GenMute™ siRNA Transfection Reagent for Neuro-2A Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNeuro-2A (N2A) cells — a mouse neuroblastoma line serving broadly as an in vitro model for neuronal differentiation, neurodevelopment, neurotoxicity, and signal transduction studies — are what the pre-optimized GenMute™ siRNA Transfection Reagent for Neuro-2A Cells addresses, delivering siRNA into them efficiently. Transfection efficiency across N2A cultures can vary when conventional reagents are used, the more so once the cells take on a neuron-like differentiated state. This formulation sets out to raise cellular uptake and endosomal release of siRNA without compromising viability or normal cellular morphology.\u003c\/p\u003e\n\u003cp\u003eOptimal gene knockdown depends on following the transfection conditions recommended for Neuro-2A cells: cell density, siRNA amount, and incubation parameters.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eNeuro-2A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of the cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e10 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899251565,"sku":"SL100568-N2A-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904068461,"sku":"SL100568-N2A-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904101229,"sku":"SL100568-N2A-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904133997,"sku":"SL100568-N2A-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mefs-bht2200027","title":"GenJet™ In Vitro DNA Transfection Reagent for MEFs","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eFeeder layers that support the growth and maintenance of undifferentiated embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are commonly built from mouse embryonic fibroblasts (MEFs). Isolated from mouse embryos, these primary fibroblasts are normally put to work at early passage numbers, and before serving as feeders they are usually arrested by irradiation or by mitomycin C treatment so that proliferation stops while their supportive functions carry on. Primary cells of this kind have a reputation for being hard to transfect.\u003c\/p\u003e\n\u003cp\u003eGenJet™ DNA In Vitro Transfection Reagent for Mouse Embryonic Fibroblasts (MEFs) arrives pre-optimized and conditioned for plasmid DNA delivery into MEFs, and SignaGen credits its proprietary transfection chemistry plus an optimized protocol with taking the reagent as high as 85% GFP-positive cells under optimized conditions.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMEFs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. Under recommended storage conditions, the product is stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300 transfections in 24-well plates or 150 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_MEF.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for MEFs – GFP transfection in mouse embryonic fibroblasts (MEFs)\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection efficiency of GenJet™ Transfection Reagent in Mouse Embryonic Fibroblasts (MEFs). MEFs were cultured under recommended growth conditions and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 24 hours post-transfection by assessing GFP expression using fluorescence microscopy.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899284333,"sku":"SL100489-MEF-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903183725,"sku":"SL100489-MEF-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903216493,"sku":"SL100489-MEF-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903249261,"sku":"SL100489-MEF-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_MEF_c18eda95-4180-4dcb-93d2-f8ccc8498f17.jpg?v=1790175947"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-siha-cells-bht2200032","title":"GenJet™ In Vitro DNA Transfection Reagent for SiHa Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eIntegrated human papillomavirus type 16 (HPV-16) DNA sits in the genome of SiHa, a human cervical squamous cell carcinoma line that is a common choice for studying cervical cancer biology, viral oncogenesis, gene regulation, signal transduction, and anticancer therapeutics. GenJet™ DNA In Vitro Transfection Reagent for SiHa Cells is tuned in advance to transfect DNA into this line efficiently, its formulation worked up to give high efficiency while SiHa cultures keep excellent viability. The protocol below was refined to push efficiency to its maximum in SiHa cells, and one milliliter of GenJet™ reagent stretches to roughly 300–600 transfections using 24-well plates, or 50–100 using 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eSiHa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899218797,"sku":"SL100489-SiHa-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903871853,"sku":"SL100489-SiHa-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903904621,"sku":"SL100489-SiHa-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903937389,"sku":"SL100489-SiHa-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-u2os-cells-bht2200053","title":"GenMute™ siRNA Transfection Reagent for U2OS Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eU2OS cells receive siRNA efficiently with GenMute™ siRNA Transfection Reagent for U2OS Cells, a formulation optimized for this line. Established in 1964, U2OS came originally from the tibial bone of a female patient aged 15 and is of human osteosarcoma origin. Its adherent, epithelial-like morphology accompanies broad use in gene function work, DNA damage response, cell cycle regulation, and cancer biology.\u003c\/p\u003e\n\u003cp\u003eReceptors for insulin-like growth factor I (IGF-I) and II (IGF-II) are expressed by U2OS cells, and the line is well characterized for growing and transfecting consistently in vitro.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eU2OS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Culture Medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Confluency on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA Concentration\u003c\/th\u003e\n\u003ctd\u003e5 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 15 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make a 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899317101,"sku":"SL100568-U2OS-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906820973,"sku":"SL100568-U2OS-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906853741,"sku":"SL100568-U2OS-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906886509,"sku":"SL100568-U2OS-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-c2c12-cells-bht2200009","title":"GenJet™ In Vitro DNA Transfection Reagent for C2C12 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eYaffe and Saxel (1977) generated the C2C12 line by serially passaging, under selection, myoblasts taken from the thigh muscle of C3H mice after a crush injury. Because these cells still differentiate into multinucleated myotubes, they serve broadly as an in vitro system for skeletal muscle development, myogenic differentiation, muscle-specific gene regulation, myosin phosphorylation pathways, and androgen receptor (AR) signaling. GenJet™ DNA In Vitro Transfection Reagent for C2C12 Cells ships with conditions already tuned for C2C12 myoblasts, so plasmid DNA is delivered efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eC2C12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. Under recommended storage conditions, the product is stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899349869,"sku":"SL100489-C2C12-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903282029,"sku":"SL100489-C2C12-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903314797,"sku":"SL100489-C2C12-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903347565,"sku":"SL100489-C2C12-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mcf10a-cells-bht2200024","title":"GenJet™ In Vitro DNA Transfection Reagent for MCF10A Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eMCF10A is an immortalized human mammary epithelial line that is not tumorigenic and holds on to much of what characterizes normal mammary epithelium. Its cells carry epithelial markers — sialomucins, cytokeratins, and milk fat globule antigens among them. Placed in collagen matrices they grow in three dimensions; at confluence they throw up dome-like structures, a sign of their capacity to differentiate along epithelial lines.\u003c\/p\u003e\n\u003cp\u003eNeither terminal differentiation nor cellular senescence has been seen in them so far under standard culture conditions. Insulin, glucocorticoids, cholera toxin, and epidermal growth factor (EGF) all elicit a response from the line, while electron microscopy of cell ultrastructure points to luminal ductal epithelial character rather than a myoepithelial one. GenJet™ DNA In Vitro Transfection Reagent for MCF10A Cells is tuned in advance to move plasmid DNA into this non-tumorigenic line efficiently: the formulation rests on SignaGen's proprietary transfection booster conjugation technology and, under optimized conditions, reaches up to 50% GFP-positive cells.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMCF10A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. Under recommended storage conditions, the product is stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899382637,"sku":"SL100489-MCF10A-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902495597,"sku":"SL100489-MCF10A-0P5ML","price":118.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902528365,"sku":"SL100489-MCF10A-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902561133,"sku":"SL100489-MCF10A-1P0MLX5","price":1036.0,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-mcf-7-cells-bht2200045","title":"GenMute™ siRNA Transfection Reagent for MCF-7 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eMCF-7, a human breast adenocarcinoma cell line in wide circulation, is the target of the pre-optimized GenMute™ siRNA Transfection Reagent for MCF-7 Cells, which moves siRNA into these cells efficiently. Isolated in 1970 from a Caucasian woman aged 69, the line holds on to much of the character of differentiated mammary epithelium — hormone responsiveness and estrogen receptor (ER) expression among those traits. Investigators turn to it for estrogen signaling, breast cancer biology, proliferation, apoptosis, and how cells answer therapy.\u003c\/p\u003e\n\u003cp\u003eOncogenic pathways such as Wnt7B are expressed here too, and the cells react to cytokines like tumor necrosis factor-α (TNF-α). Low cytotoxicity alongside high transfection efficiency makes dependable gene silencing and in vitro functional studies possible.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMCF-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Culture Medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Confluency on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA Concentration\u003c\/th\u003e\n\u003ctd\u003e10 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make a 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899480941,"sku":"SL100568-MCF7-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904265069,"sku":"SL100568-MCF7-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904297837,"sku":"SL100568-MCF7-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904330605,"sku":"SL100568-MCF7-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-hela-cells-bht2200040","title":"GenMute™ siRNA Transfection Reagent for HeLa Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eFew human cervical cancer cell lines see as much biomedical research use as HeLa, which turns up throughout work on gene function, cancer biology, signal transduction, virology, and drug discovery — yet general-purpose reagents can struggle to give consistent knockdown without toxicity. GenMute™ siRNA Transfection Reagent for HeLa Cells answers that with conditions tuned specifically in advance for efficient siRNA delivery into these cells. Engineering of the formulation targets maximal cellular uptake and intracellular release of siRNA, with cell viability and normal morphology preserved.\u003c\/p\u003e\n\u003cp\u003eTransfection efficiency runs high and cytotoxicity low, so gene silencing comes out robust and reproducible for downstream in vitro applications: pathway analysis, target validation, functional assays.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHeLa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell confluency on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e10.0 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899448173,"sku":"SL100568-HELA-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905117037,"sku":"SL100568-HELA-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905149805,"sku":"SL100568-HELA-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905182573,"sku":"SL100568-HELA-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"pepjet-in-vivo-dna-transfection-reagent-bht2200035","title":"PepJet™ In Vivo DNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eAt the heart of PepJet™ In Vivo DNA Transfection Reagent sits a synthetic 28-amino-acid peptide, picked out originally by screening as a potent nucleic acid delivery vehicle and then refined with sequence alignment, molecular modeling, and computational analyses that took their cue from naturally occurring viral protein architectures. Chemical synthesis, structural refinement, and repeated rounds of in vitro and in vivo validation followed, all aimed at wringing the most gene transfer efficiency out of it; what resulted is a next-generation cationic peptide–based delivery system engineered for highly efficient plasmid DNA transfection in vivo. A proprietary shielding peptide is covalently built into the platform for better systemic stability and biological performance: it cuts down nonspecific interactions with serum proteins and other biological components once the material is given systemically, so PepJet™\/DNA complexes form, hold together, and distribute better.\u003c\/p\u003e\n\u003cp\u003eAnimal studies using systemic administration have shown efficient plasmid DNA delivery and robust transgene expression across several organs — lung, liver, spleen, and kidney.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eIn Vivo Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vivo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, PepJet™ DNA In Vivo Transfection Reagent is stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepJet_In_Vivo.webp?v=1790173498\" alt=\"PepJet™ In Vivo DNA Transfection Reagent – Renilla luciferase in mouse lung, liver, spleen and kidney after systemic delivery\" loading=\"lazy\"\u003e\u003cfigcaption\u003eThe in vivo transfection efficiency of PepJet™ reagent was evaluated in mice using a plasmid encoding Renilla luciferase as a reporter gene. Following systemic administration of PepJet™\/DNA complexes, transgene expression levels were quantified in various organs to assess tissue distribution and gene delivery efficiency. PepJet™ mediated robust Renilla luciferase expression in the lung, liver, spleen, and kidney, demonstrating its effectiveness as a systemic plasmid DNA delivery platform.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eSuitable for in vivo gene delivery applications including gene therapy research, in vivo reporter assays, and preclinical studies. Consult the product datasheet for recommended dosing and administration routes.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899415405,"sku":"SL100501-0P1ML","price":29.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904559981,"sku":"SL100501-0P5ML","price":146.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904592749,"sku":"SL100501-1P0ML","price":278.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904625517,"sku":"SL100501-1P0MLX5","price":1320.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepJet_In_Vivo_cadb97c7-edda-4c65-bd26-0ee74feddc5b.webp?v=1790175948"},{"product_id":"genmute-sirna-transfection-reagent-for-mefs-bht2200046","title":"GenMute™ siRNA Transfection Reagent for MEFs","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eEfficient siRNA delivery into mouse embryonic fibroblasts (MEFs) is what GenMute™ siRNA Transfection Reagent for MEFs was optimized to achieve. A frequent role for MEFs is as feeder cells, sustaining undifferentiated mouse or human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Taken from mouse embryos and usually kept at early passages, they contribute the growth factors and extracellular support such cultures require.\u003c\/p\u003e\n\u003cp\u003eIrradiation, or treatment with mitomycin C, arrests their growth ahead of feeder-layer use, halting division yet leaving the supportive functions intact.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMEFs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of the cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1xGenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899513709,"sku":"SL100568-MEF-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906526061,"sku":"SL100568-MEF-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906558829,"sku":"SL100568-MEF-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906591597,"sku":"SL100568-MEF-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-lncap-cells-bht2200022","title":"GenJet™ In Vitro DNA Transfection Reagent for LNCaP Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eDerived from a lymph node metastasis, LNCaP is an androgen-responsive human prostate adenocarcinoma cell line that has become a staple of prostate cancer research, androgen receptor signaling studies, and drug discovery. Morphologically the cells look epithelial, and in culture they tend to appear both as aggregates and as single adherent cells. GenJet™ DNA In Vitro Transfection Reagent for LNCaP Cells arrives with conditions already tuned for this line, so DNA transfection runs efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eLNCaP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_LNCAP.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for LNCaP Cells – GFP transfection in LNCaP cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection efficiency of GenJet™ Transfection Reagent (Ver. II), pre-optimized for plasmid DNA delivery into LNCaP cells. LNCaP cells were cultured under ATCC-recommended growth conditions and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 48 hours post-transfection by assessing GFP expression via fluorescence microscopy.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899612013,"sku":"SL100489-LNCaP-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901414253,"sku":"SL100489-LNCaP-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901447021,"sku":"SL100489-LNCaP-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901479789,"sku":"SL100489-LNCaP-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_LNCAP_88560016-72b6-48b9-bce9-303ee348291c.jpg?v=1790175947"},{"product_id":"lipojet-transfection-buffer-5x-bht2200037","title":"LipoJet™ Transfection Buffer (5x)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eLipoJet™ Transfection Buffer was formulated for use with LipoJet™ siRNA \u0026amp; DNA Transfection Reagent, supporting optimal transfection efficiency and maximum knockdown of gene expression. The buffer is used to dilute siRNA\/DNA and LipoJet™ Transfection Reagent so that the most efficient transfection complex can form. LipoJet™ Transfection Buffer(5x), 8.0 mL, is supplied as a 5x concentrated stock solution that yields 40 mL of working solution.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eBuffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at RT. If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eLipoJet™ Transfection Buffer, 8.0 mL\/vial, to make 40 mL of working solution (1x ), sufficient for 1000 transfections in 24-well plate\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis transfection buffer is used to prepare transfection complexes with the matching SignaGen transfection reagent, for both DNA and siRNA delivery. Use it to dilute the nucleic acid and the reagent as described in the protocol.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"1.0 mL","offer_id":54042899546477,"sku":"SL100567-1P0ML","price":5.0,"currency_code":"USD","in_stock":true},{"title":"4.0 mL","offer_id":54042905969005,"sku":"SL100567-4P0ML","price":20.0,"currency_code":"USD","in_stock":true},{"title":"8.0 mL","offer_id":54042906001773,"sku":"SL100567-8P0ML","price":39.0,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-ver-ii-bht2200004","title":"GenJet™ In Vitro DNA Transfection Reagent (Ver. II)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eGenJet™ In Vitro DNA Transfection Reagent (Ver. II) upgrades the original GenJet™ In Vitro DNA Transfection Reagent through a reformulated chemistry. That chemistry liberates DNA-condensing groups at 100%, where the earlier version reached 80%, and transfection efficiency climbs 3–4 fold as a result, with cell viability left intact.\u003c\/p\u003e\n\u003cp\u003eCoverage per 1.0 mL vial of GenJet™ (Ver. II) Reagent runs to about 667 transfections at 24-well scale, or 333 at 6-well scale.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under recommended conditions, the product remains stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e3–4 fold higher transfection efficiency, depending on cell type\u003c\/li\u003e\n\u003cli\u003ePreferred option for hard-to-transfect cells\u003c\/li\u003e\n\u003cli\u003eImproved transfection efficiency with serum and antibiotics present, for most cell types\u003c\/li\u003e\n\u003cli\u003eSupports exceptionally high levels of recombinant protein expression\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – luciferase efficiency and MTT toxicity vs original GenJet™\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of transfection efficiency (left panel) and toxicity (right panel) GenJet™ Ver. II with the old version of GenJet™ on commonly used mammalian cells. phRL-CMV encoding Renilla Luciferase was introduced into cells in the presence of serum and antibiotics. The Renilla Luciferase activity was determined with the Renilla Luciferase Assay System (Promega). For cytotoxicity, the MTT assay was used to determine cell viability, with the untransfected control set at 100%.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HartoCell2.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – nuclear GFP in primary rat aortic smooth muscle cells vs L2K\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of the transfection efficiency of GenJet™ (Ver. II) vs. Lipofectamine 2000 on primary rat aortic smooth muscle cells. Primary rat aortic smooth muscle cells were prepared and transfected with a nuclear-targeted GFP cDNA by GenJet™ (right panel) and Lipofectamine 2000 (L2K, left panel), respectively. The transfection efficiency was checked under a Nikon Eclipse 2000 fluorescence microscope 48 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-2.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – GFP transfection vs original GenJet™ in 293T and HeLa cells\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-3II.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – GFP transfection vs original GenJet™ in 293T and HeLa cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eUpper Panel: Transfection efficiency comparison of GenJet™ Ver. II with the old version of GenJet™ on 293T cells. pEGFP-N3 was transfected into 293T cells at 90% confluency in the presence of serum and antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48 hours post-transfection Lower panel: Transfection efficiency comparison of GenJet™ Ver. II with the old version of GenJet™ on Hela cells. pEGFP-N3 was transfected into Hela cells at 90% in the presence of serum and antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899579245,"sku":"SL100489-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901709165,"sku":"SL100489-0P5ML","price":96.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901741933,"sku":"SL100489-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901774701,"sku":"SL100489-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-2_3ff4b1c8-4715-4da3-82a4-dbc788b3c529.jpg?v=1790175949"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-b16-f10-cells-bht2200007","title":"GenJet™ In Vitro DNA Transfection Reagent for B16-F10 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe B16-F10 line is a highly metastatic murine melanoma, derived from the B16 melanoma that was first established in C57BL\/6 mice, and laboratories turn to it routinely as an experimental model in melanoma biology, tumor metastasis, cancer immunology, and work on novel anticancer therapies. Several melanoma-associated antigens are expressed by these cells — tyrosinase and the tyrosinase-related proteins TRP-1 and TRP-2 among them, alongside other tumor-associated antigens — which makes the line a valuable platform for evaluating cancer vaccines, immunotherapies, and gene-based therapeutic approaches. GenJet™ DNA In Vitro Transfection Reagent for B16-F10 Cells comes tuned to move plasmid DNA into B16-F10 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eB16-F10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899644781,"sku":"SL100489-B16-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902790509,"sku":"SL100489-B16-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902823277,"sku":"SL100489-B16-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902856045,"sku":"SL100489-B16-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-cos-cells-bht2200012","title":"GenJet™ In Vitro DNA Transfection Reagent for COS Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eImmortalized from African green monkey kidney, COS cells are fibroblast-like and constitutively express the SV40 large T antigen; plasmids carrying the SV40 origin of replication can therefore replicate episomally inside them. That property explains their broad use for transient gene expression, recombinant protein production, and molecular, biochemical, and cell biology studies of many kinds. Of the derivatives of the original COS cell line, COS-1 and COS-7 are the two seen most often, and GenJet™ DNA In Vitro Transfection Reagent for COS Cells comes tuned for both, delivering DNA into COS-1 and COS-7 cells at high efficiency.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eCOS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under the recommended conditions, the product is stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-COS1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for COS Cells – GFP transfection in COS-1 cells\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-COS7.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for COS Cells – GFP transfection in COS-7 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eTwo Representative Images Showing the Transfection Efficiency of GenJet™ Reagent in COS-1 (Upper Panel) and COS-7 (Lower Panel) Cells. COS-1 and COS-7 cells were cultured according to ATCC-recommended conditions and transfected with the pEGFP-N3 expression plasmid using GenJet™ reagent. GFP expression was evaluated 24 hours post-transfection to assess transfection efficiency.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899677549,"sku":"SL100489-COS-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902397293,"sku":"SL100489-COS-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902430061,"sku":"SL100489-COS-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902462829,"sku":"SL100489-COS-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-COS1_58dc4a32-9fd3-49e6-9f8e-cff04ea52f39.jpg?v=1790175945"},{"product_id":"genmute-sirna-transfection-reagent-for-sh-sy5y-cells-bht2200052","title":"GenMute™ siRNA Transfection Reagent for SH-SY5Y Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eSH-SY5Y human neuroblastoma cells receive siRNA efficiently through GenMute™ siRNA Transfection Reagent for SH-SY5Y Cells, which was optimized for this line. Its broad use as an in vitro neuronal model rests on the option of holding the cells undifferentiated or pushing them toward a neuron-like phenotype with agents such as brain-derived neurotrophic factor (BDNF) or retinoic acid. Drug discovery, neurotoxicity, neurodegenerative diseases, synaptic function, and neuronal development are among the questions pursued with them.\u003c\/p\u003e\n\u003cp\u003eBoth proliferating and differentiated SH-SY5Y cultures keep viability high under the optimized formulation while robust gene knockdown is achieved.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eSH-SY5Y\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003e86%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899710317,"sku":"SL100568-SY5Y-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906231149,"sku":"SL100568-SY5Y-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906263917,"sku":"SL100568-SY5Y-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906296685,"sku":"SL100568-SY5Y-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-insulinoma-cells-bht2200042","title":"GenMute™ siRNA Transfection Reagent for Insulinoma Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eβ cells of the pancreas normally put out insulin when blood glucose climbs, then ease off once glucose settles back to baseline. INS cells behave differently: these tumor-derived pancreatic β-cell lines, a common tool for studying insulin secretion and β-cell biology, frequently show insulin secretion that glucose levels fail to hold in check, so release keeps going and glucose concentrations fall abnormally low. GenMute™ siRNA Transfection Reagent for Insulinoma (INS) Cells arrives tuned in advance to deliver siRNA into INS cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eInsulinoma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 12 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eRPMI 1640 with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 Hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899775853,"sku":"SL100568-INS-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904461677,"sku":"SL100568-INS-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904494445,"sku":"SL100568-INS-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904527213,"sku":"SL100568-INS-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-plus-in-vivo-dna-transfection-reagent-bht2200034","title":"GenJet™ Plus In Vivo DNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eGenJet™ Plus In Vivo DNA Transfection Reagent grew out of the original GenJet™ DNA Transfection Reagent: an advanced non-viral gene delivery system, further refined by working in several proprietary cationic and functional peptides. Those additions, SignaGen says, sharply raise how densely DNA-binding and condensing moieties are packed, which in turn strengthens plasmid DNA complexation and stability and lifts delivery efficiency in vivo. Bolted on covalently, a shielding peptide holds down nonspecific interactions with serum components and other biological barriers, so circulation through the body and transfection performance in vivo both improve.\u003c\/p\u003e\n\u003cp\u003eFollowing systemic administration, GenJet™ Plus has been shown to drive robust transgene expression, with Renilla luciferase activity registering high in several organs — lung, liver, spleen, and kidney.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eIn Vivo Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vivo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 2–8°C. Under the recommended storage conditions, GenJet™ Plus DNA In Vivo Transfection Reagent remains stable for a minimum of 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-Vivo-1.webp?v=1790173498\" alt=\"GenJet™ Plus In Vivo DNA Transfection Reagent – Renilla luciferase expression in mouse organs after systemic delivery\" loading=\"lazy\"\u003e\u003cfigcaption\u003eThe in vivo transfection efficiency of GenJet™ Plus reagent was evaluated in mice using a plasmid encoding Renilla luciferase as a reporter gene. Following systemic administration, transgene expression was quantified in multiple organs and compared with alternative delivery approaches. GenJet™ Plus consistently demonstrated efficient plasmid DNA delivery and strong transgene expression across a broad range of tissues, confirming its suitability for systemic in vivo gene transfer applications.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eSuitable for in vivo gene delivery applications including gene therapy research, in vivo reporter assays, and preclinical studies. Consult the product datasheet for recommended dosing and administration routes.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899743085,"sku":"SL100500-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906427757,"sku":"SL100500-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906460525,"sku":"SL100500-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906493293,"sku":"SL100500-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-Vivo-1_b039bd24-3232-49b1-ba50-a4659d34b87d.webp?v=1790175949"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-pc-3-cells-bht2200031","title":"GenJet™ In Vitro DNA Transfection Reagent for PC-3 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePC-3, a human prostate adenocarcinoma line taken from a bone metastasis, is a common choice for studies of prostate cancer progression, metastasis, signal transduction, and therapeutic development. Transfecting these cells efficiently is possible, but they often react badly to the cytotoxicity that comes with conventional lipid-based transfection reagents — viability drops and experimental outcomes suffer. GenJet™ DNA In Vitro Transfection Reagent for PC-3 Cells is tuned in advance for efficient DNA transfection of the line; SignaGen attributes to its proprietary formulation and optimization the pairing of highly efficient plasmid DNA delivery with minimal transfection-associated cytotoxicity.\u003c\/p\u003e\n\u003cp\u003eUp to 60% GFP-positive cells is attainable under optimized conditions, with viability staying excellent. The protocol below was refined to reach maximal efficiency in PC-3 cells. One milliliter of GenJet™ reagent stretches to roughly 300–600 transfections in 24-well plates, or 50–100 in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003ePC-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899841389,"sku":"SL100489-PC3-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903970157,"sku":"SL100489-PC3-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904002925,"sku":"SL100489-PC3-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904035693,"sku":"SL100489-PC3-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-kit-for-huvec-bht2200019","title":"GenJet™ In Vitro DNA Transfection Kit for HUVEC","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eHuman Umbilical Vein Endothelial Cells (HUVECs) are primary endothelial cells that researchers rely on across angiogenesis, vascular biology, endothelial signaling, inflammation, and cardiovascular disease work. Being primary in origin, and being sensitive to the cytotoxicity that accompanies transfection, HUVECs are usually regarded as hard targets for conventional non-viral gene delivery methods. GenJet™ DNA In Vitro Transfection Reagent for HUVECs was tuned to that problem: paired with GenJet™ Transfection Buffer, the specialized formulation raises DNA uptake and transgene expression while holding transfection-related cytotoxicity down, and optimized conditions can reach up to 60% transfection efficiency with excellent cell viability preserved.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHUVEC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 500 transfections in 24-well plates or 250 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899874157,"sku":"SL100489-HUVEC-0P1ML","price":40.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903576941,"sku":"SL100489-HUVEC-0P5ML","price":206.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903609709,"sku":"SL100489-HUVEC-1P0ML","price":398.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903642477,"sku":"SL100489-HUVEC-1P0MLX5","price":1890.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-sirna-transfection-reagent-for-jurkat-cells-bht2200043","title":"GenMute™ siRNA Transfection Reagent for Jurkat Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eJurkat cells — an immortalized human T-lymphocyte cell line that immunology and cancer research draw on heavily — are the target of GenMute™ siRNA Transfection Reagent for Jurkat Cells, which arrives pre-optimized so that siRNA reaches them efficiently. Much of that research treats the line as a key model: acute T-cell leukemia, signaling through the T-cell receptor, apoptosis, and how chemokine receptors tied to viral entry, HIV among them, are expressed. Interleukin-2 (IL-2) production and immune activation pathways are studied in them as well.\u003c\/p\u003e\n\u003cp\u003eWithin this suspension format the reagent pairs high transfection efficiency with minimal cytotoxicity, a fit for gene silencing work, pathway analysis, and functional in vitro assays.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eJurkat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Culture Medium\u003c\/th\u003e\n\u003ctd\u003e1640 Medium with 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell confluency on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA Concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 10 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make a 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899808621,"sku":"SL100568-JURKAT-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042906132845,"sku":"SL100568-JURKAT-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042906165613,"sku":"SL100568-JURKAT-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042906198381,"sku":"SL100568-JURKAT-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-bht2200003","title":"GenJet™ In Vitro DNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eA unique chemistry sits behind GenJet™ DNA In Vitro Transfection Reagent: cationic lipids are covalently cross-linked to polymers, and SignaGen credits that design for exceptional performance and clearly distinguishable advantages next to conventional transfection reagents. The product is powerful and reliable, reaching high transfection efficiency while cytotoxicity stays minimal and is frequently undetectable. Gene delivery has been demonstrated across many established lines and primary cells — HEK293, 293T, 293E, CHO, COS-1, HeLa and NIH\/3T3 among them, plus the insect lines Sf9 and Sf21 and numerous further eukaryotic cell types.\u003c\/p\u003e\n\u003cp\u003eEach 1.0 mL vial of GenJet™ Reagent handles roughly 666 transfections at 24-well scale, or 333 at 6-well scale.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under recommended conditions, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSupports exceptionally high viral titers\u003c\/li\u003e\n\u003cli\u003eHighly effective with large DNA constructs, up to 56 kb\u003c\/li\u003e\n\u003cli\u003eWorks equally well for single transfection or co-transfection of multiple DNA constructs\u003c\/li\u003e\n\u003cli\u003eEnables exceptionally high levels of recombinant protein expression\u003c\/li\u003e\n\u003cli\u003eWell suited to hard-to-transfect cell types\u003c\/li\u003e\n\u003cli\u003eCost-effective transfection option\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-LongDNA.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – 14 kb GFP-tagged DNA transfection in HEK293 cells vs FuGene™ 6\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExceptional transfection efficiency of extremely long DNA by GenJet™ DNA In Vitro Transfection Reagent. HEK293 cells were transfected with GFP-tagged DNA (total 14 kb) by GenJet™ (upper panel) and FuGene™ 6 (lower panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-Lentivirus.gif?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – lentivirus titers from 293F cells vs Lipofectamine 2000 by FACS\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExceptionally high titers of lentivirus were generated from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter virus supernatant from 293F cells was used to counter-infect 293F cells, which were then passed through FACS. The numbers in the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ and L2K were quantified to be 7.9×106 and 3.1×106 tu\/mL, respectively\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet3.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – GFP transfection in primary murine skin fibroblasts, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-FuGene1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – primary murine skin fibroblasts transfected with FuGene™ HD for comparison\" loading=\"lazy\"\u003e\u003cfigcaption\u003ePrimary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and the most popular brand product FuGene™ HD (lower panel). The cells were visualized by a Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-KE.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – GFP transfection in primary murine keratinocytes, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-FuGene-KE.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – primary murine keratinocytes transfected with FuGene™ HD for comparison\" loading=\"lazy\"\u003e\u003cfigcaption\u003ePrimary Murine Keratinocytes transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and one of the most popular brand products, FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-Toxicity1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – cytotoxicity vs L2K™ in primary murine skin fibroblasts, DIC imaging\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents\/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium, followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch3\u003eTransfection Efficiency by Cell Line\u003c\/h3\u003e\n\u003ctable class=\"bhp-spec-table bhp-data-table\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCell Lines\u003c\/th\u003e\n\u003cth\u003eTransfection Efficiency (% GFP)\u003c\/th\u003e\n\u003cth\u003eCell Lines\u003c\/th\u003e\n\u003cth\u003eTransfection Efficiency (% GFP)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e3LL\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003ctd\u003eK562\u003c\/td\u003e\n\u003ctd\u003e22%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eB16-F10\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003ctd\u003eL929\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBAEC\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003ctd\u003eMCF-7\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBHK-21\u003c\/td\u003e\n\u003ctd\u003e86%\u003c\/td\u003e\n\u003ctd\u003eMDCK\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCa Ski\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003ctd\u003eNeuro2A\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCaCo-2\u003c\/td\u003e\n\u003ctd\u003e32%\u003c\/td\u003e\n\u003ctd\u003eNIH 3T3\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003ctd\u003e83%\u003c\/td\u003e\n\u003ctd\u003ePC12\u003c\/td\u003e\n\u003ctd\u003e34%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHCS-2\/8\u003c\/td\u003e\n\u003ctd\u003e42%\u003c\/td\u003e\n\u003ctd\u003eSH-SY5Y\u003c\/td\u003e\n\u003ctd\u003e14%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHEK-293\u003c\/td\u003e\n\u003ctd\u003e80-90%\u003c\/td\u003e\n\u003ctd\u003eSiHa\u003c\/td\u003e\n\u003ctd\u003e62%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeLa\u003c\/td\u003e\n\u003ctd\u003e81%\u003c\/td\u003e\n\u003ctd\u003eSKOV3\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHLMEC\u003c\/td\u003e\n\u003ctd\u003e50-65%\u003c\/td\u003e\n\u003ctd\u003eHUVEC\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH-MVEC\u003c\/td\u003e\n\u003ctd\u003e49%\u003c\/td\u003e\n\u003ctd\u003eIGROV1\u003c\/td\u003e\n\u003ctd\u003e21%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuh-7D12\u003c\/td\u003e\n\u003ctd\u003e22%\u003c\/td\u003e\n\u003ctd\u003eJurkat\u003c\/td\u003e\n\u003ctd\u003e5%-15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATT20\u003c\/td\u003e\n\u003ctd\u003e38%\u003c\/td\u003e\n\u003ctd\u003e6CSFMEo\u003c\/td\u003e\n\u003ctd\u003e73%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSK-N-SH\u003c\/td\u003e\n\u003ctd\u003e20%\u003c\/td\u003e\n\u003ctd\u003eWEHI 231\u003c\/td\u003e\n\u003ctd\u003e25%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMcArdle 7777\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003ctd\u003eSAOS-2\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHep3D\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003ctd\u003eSN56\u003c\/td\u003e\n\u003ctd\u003e69%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSHEP\u003c\/td\u003e\n\u003ctd\u003e71%\u003c\/td\u003e\n\u003ctd\u003eMC3T3-E1\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMDA-MB-231\u003c\/td\u003e\n\u003ctd\u003e38%\u003c\/td\u003e\n\u003ctd\u003eBT474\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3T3-442A\u003c\/td\u003e\n\u003ctd\u003e10%\u003c\/td\u003e\n\u003ctd\u003eC2C12\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCOS-7\u003c\/td\u003e\n\u003ctd\u003e60-70%\u003c\/td\u003e\n\u003ctd\u003ePrimary melanocyte\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCV-1\u003c\/td\u003e\n\u003ctd\u003e53%\u003c\/td\u003e\n\u003ctd\u003eHela-S3\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eD 407\u003c\/td\u003e\n\u003ctd\u003e62%\u003c\/td\u003e\n\u003ctd\u003eA549\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDHD Pro.b\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003ctd\u003eHep G2\u003c\/td\u003e\n\u003ctd\u003e58%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLS180\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEfficient DNA delivery into mammalian and insect cells\u003c\/li\u003e\n\u003cli\u003eDramatic enhancement of viral infection, up to 200-fold, including replication-deficient adenovirus, in hard-to-transfect cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899906925,"sku":"SL100488-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901512557,"sku":"SL100488-0P5ML","price":88.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901545325,"sku":"SL100488-1P0ML","price":166.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901578093,"sku":"SL100488-1P0MLX5","price":788.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-LongDNA_b817c0d2-cf9c-44af-8652-3445b6ba166c.jpg?v=1790175948"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-u2os-cells-bht2200030","title":"GenJet™ In Vitro DNA Transfection Reagent for U2OS Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eU2OS began with tibial tumor tissue taken from a 15-year-old female patient; the human osteosarcoma line that resulted now ranks among the most heavily used cell models in cancer biology and molecular genetics. The cells attach and adopt an epithelial-like shape, and they turn up throughout work on cell cycle regulation, DNA damage response, genome stability, signal transduction, gene expression, and cancer therapeutics. p53 function in the line remains wild-type, which is what makes it so useful for digging into tumor suppression mechanisms and cellular stress responses. GenJet™ DNA In Vitro Transfection Reagent for U2OS Cells arrives with its conditions already tuned, so DNA transfection of U2OS cells runs efficiently; the protocol below was refined to reach maximal efficiency in these cells while viability stays excellent.\u003c\/p\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent stretches to roughly 300–600 transfections in 24-well plates, or 150–300 in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eU2OS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-OS_png.webp?v=1790173498\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for U2OS Cells – GFP transfection in U2OS cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA Representative Image Demonstrating the Transfection Efficiency of GenJet™ Reagent in U2OS Cells. U2OS cells were cultured according to ATCC-recommended growth conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 48 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899939693,"sku":"SL100489-OS-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904658285,"sku":"SL100489-OS-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904691053,"sku":"SL100489-OS-1P0ML","price":189.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904723821,"sku":"SL100489-OS-1P0MLX5","price":897.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-OS_png_40bb99ad-9172-4776-a203-ced43d374250.webp?v=1790175947"},{"product_id":"genmute-sirna-transfection-reagent-for-primary-macrophages-bht2200048","title":"GenMute™ siRNA Transfection Reagent for Primary Macrophages","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePrimary macrophages take up siRNA efficiently with GenMute™ siRNA Transfection Reagent for PMG, optimized for exactly that purpose. Circulating monocytes give rise to these tissue-resident white blood cells, whose roles sit at the center of innate as well as adaptive immunity. Their work as professional phagocytes involves engulfing and breaking down foreign particles, pathogens, and cellular debris, alongside secretion of the cytokines that steer immune responses.\u003c\/p\u003e\n\u003cp\u003eA characteristic marker set — CD14, CD11b, F4\/80 (mouse) or EMR1 (human), Lysozyme M, MAC-1\/MAC-3, and CD68 — allows their identification through flow cytometry or immunohistochemical staining.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003ePrimary Macrophages\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900169069,"sku":"SL100568-PMG-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905018733,"sku":"SL100568-PMG-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905051501,"sku":"SL100568-PMG-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905084269,"sku":"SL100568-PMG-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-huh-7-cells-bht2200018","title":"GenJet™ In Vitro DNA Transfection Reagent for HuH-7 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eHuH-7, a human hepatocellular carcinoma line, turns up often in liver biology, virology, and gene expression studies. Transfecting it is generally harder than transfecting HEK293-derived cells; proprietary peptide-conjugation technology built into GenJet™ reagent addresses that, driving plasmid DNA in at high efficiency and reaching as much as 70% GFP-positive cells under optimized conditions. GenJet™ DNA In Vitro Transfection Reagent for HuH-7 Cells therefore ships pre-tuned for transfection of human HuH-7 cells.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHuH-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HUH7.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HuH-7 Cells – GFP transfection vs L2K and FuGENE® HD; serum\/antibiotic effect\" loading=\"lazy\"\u003e\u003cfigcaption\u003eGenJet™ reagent is specifically optimized for HuH-7 cells and demonstrates superior transfection performance compared with Lipofectamine™ 2000 (L2K) and FuGENE® HD. HuH-7 cells were cultured according to recommended conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 48 hours post-transfection. Right Panel: Comparison of transfection efficiency among GenJet™, Lipofectamine™ 2000 (L2K), and FuGENE® HD in HuH-7 cells. Cells were transfected with pEGFP-N3 according to each manufacturer’s recommended protocol. The percentage of GFP-positive cells and relative fluorescence intensity were quantified by flow cytometry (FACS) 48 hours post-transfection. Left Panel: The presence of serum and antibiotics enhances GenJet™-mediated transfection efficiency in HuH-7 cells. HuH-7 cells cultured on collagen-coated dishes were transfected under three different conditions: (1) serum- and antibiotic-free medium; (2) medium containing 10% serum and antibiotics, with medium replacement 5 hours post-transfection; and (3) medium containing 10% serum and antibiotics without medium replacement. Transfection efficiency was assessed 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HUH.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HuH-7 Cells – GFP transfection in HuH-7 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative fluorescence microscopy image demonstrating the transfection efficiency of the pre-optimized GenJet™ Reagent in HuH-7 cells. HuH-7 cells were cultured under recommended growth conditions on collagen type I–coated culture dishes and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ Reagent. Transgene expression was assessed 24 hours post-transfection by fluorescence imaging with a Nikon Eclipse 2000 microscope. GFP fluorescence served as an indicator of plasmid DNA delivery and gene expression efficiency.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899972461,"sku":"SL100489-HUH-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901905773,"sku":"SL100489-HUH-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901938541,"sku":"SL100489-HUH-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901971309,"sku":"SL100489-HUH-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HUH7_e8132042-86e0-41e7-9924-01f6a6c50a60.jpg?v=1790175947"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mcf-7-cells-bht2200025","title":"GenJet™ In Vitro DNA Transfection Reagent for MCF-7 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eDating to 1970, MCF-7 was established from the pleural effusion of a patient with metastatic breast cancer and is now a thoroughly characterized human breast adenocarcinoma line. It is estrogen receptor-positive (ER+), holds on to much of what marks differentiated mammary epithelium, and turns up constantly in work on breast cancer biology, hormone signaling, gene regulation, and anticancer drug development. For this line, GenJet™ DNA In Vitro Transfection Reagent for MCF-7 Cells comes with its conditions already tuned, so DNA transfection runs efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMCF-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_MCF7.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for MCF-7 Cells – GFP transfection in MCF-7 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection performance of GenJet™ reagent optimized for MCF-7 cells. MCF-7 A2 cells were cultured according to ATCC-recommended growth conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 36 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900005229,"sku":"SL100489-MCF7-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903773549,"sku":"SL100489-MCF7-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903806317,"sku":"SL100489-MCF7-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903839085,"sku":"SL100489-MCF7-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_MCF7_0bd65dd9-5792-43d2-a684-0abf29881294.jpg?v=1790175947"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-hepg2-cells-bht2200017","title":"GenJet™ In Vitro DNA Transfection Reagent for HepG2 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eHepG2, a well-characterized human hepatocellular carcinoma line, was taken from liver tissue of a 15-year-old male donor whose hepatocellular carcinoma was differentiated. Epithelial morphology and the retention of many liver-specific functions have pushed the line to the front rank of in vitro models for hepatic metabolism, gene regulation, drug discovery, toxicology, viral infection, and recombinant protein expression. Plasma proteins synthesized and secreted by HepG2 cells include albumin, transferrin, α1-antitrypsin, α2-macroglobulin, and plasminogen, and the cells answer a variety of hormonal and metabolic stimuli.\u003c\/p\u003e\n\u003cp\u003eRobust growth characteristics plus adaptability to large-scale culture systems have widened their use further across biomedical and pharmaceutical research. GenJet™ DNA In Vitro Transfection Reagent for HepG2 Cells comes tuned to the line, delivering plasmid DNA into HepG2 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHepG2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HepG2.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HepG2 Cells – HepG2 GFP transfection vs L2K and Amaxa®; serum\/antibiotic effect\" loading=\"lazy\"\u003e\u003cfigcaption\u003eGenJet™ Reagent is specifically optimized for HepG2 cells (ATCC® HB-8065™) and demonstrates superior transfection performance compared with Lipofectamine™ 2000 (L2K) and the Amaxa® electroporation system. HepG2 cells were cultured according to ATCC-recommended conditions on collagen type I–coated culture dishes and transfected with the GFP reporter plasmid pEGFP-N3. Transfection efficiency was evaluated 48 hours post-transfection. Right Panel: Comparison of transfection efficiencies achieved with GenJet™, Lipofectamine™ 2000 (L2K), and the Amaxa® electroporation system in HepG2 cells. A 4.7-kb GFP expression plasmid (pEGFP-N3) was delivered into HepG2 cells cultured on collagen-coated dishes according to each manufacturer’s recommended protocol. Transfection efficiency, expressed as the percentage of GFP-positive cells, and GFP fluorescence intensity were quantified by flow cytometry (FACS) 48 hours post-transfection. Left Panel: Effect of serum and antibiotics on GenJet™-mediated transfection in HepG2 cells. Cells cultured on collagen-coated dishes were transfected under three different conditions: (1) serum- and antibiotic-free medium; (2) medium containing 10% serum and antibiotics, followed by medium replacement 5 hours post-transfection; and (3) medium containing 10% serum and antibiotics without medium replacement following transfection. The presence of serum and antibiotics was found to enhance GenJet™-mediated gene delivery and transgene expression in HepG2 cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-HepG2-GFP-betaGal.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HepG2 Cells – GFP and β-galactosidase expression in HepG2 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eRepresentative images demonstrating the transfection performance of the pre-optimized GenJet™ DNA Transfection Reagent in HepG2 cells (ATCC® HB-8065™). HepG2 cells were cultured under ATCC-recommended conditions on collagen type I–coated culture dishes and transfected with either the GFP reporter plasmid pEGFP-N3 (4.7 kb; right panel) or the β-galactosidase expression plasmid pSV-β-galactosidase (6.9 kb; left panel). Transgene expression was evaluated 48 hours post-transfection by confocal fluorescence microscopy using a Zeiss LSM 510 system for GFP detection and by β-galactosidase staining for assessment of β-galactosidase expression. The results demonstrate efficient delivery and expression of both reporter genes in HepG2 cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900136301,"sku":"SL100489-HEPG2-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902888813,"sku":"SL100489-HEPG2-0P5ML","price":99.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902921581,"sku":"SL100489-HEPG2-1P0ML","price":189.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902954349,"sku":"SL100489-HEPG2-1P0MLX5","price":897.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HepG2_75264513-94f9-4382-b9bc-8039793d2417.jpg?v=1790175947"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-nmumg-cells-bht2200029","title":"GenJet™ In Vitro DNA Transfection Reagent for NMuMG Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNMuMG (Normal Murine Mammary Gland) cells make up a non-transformed mouse mammary epithelial line that turns up often in work on epithelial biology, cell adhesion, growth factor signaling, epithelial-to-mesenchymal transition (EMT), and cellular differentiation. Among the cell-surface proteoglycans they display are the syndecans, and the line is a valuable system for asking how cell proliferation, extracellular matrix interactions, and growth factor-mediated signaling pathways are regulated. Since these are normal epithelial cells, the cytotoxicity that comes with transfection can hit them harder than it hits many transformed lines.\u003c\/p\u003e\n\u003cp\u003eGenJet™ DNA In Vitro Transfection Reagent for NMuMG Cells is tuned in advance for efficient DNA transfection of this line, the formulation worked up specifically to push efficiency as high as it will go while viability stays excellent. The protocol below was likewise refined for maximal transfection efficiency in NMuMG cells. One milliliter of GenJet™ reagent stretches to roughly 300–600 transfections in 24-well plates, or 150–300 in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eNMuMG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-NMUMG.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for NMuMG Cells – GFP transfection in NMuMG cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA Representative Image Demonstrating the Transfection Efficiency of GenJet™ Reagent in NMuMG Cells. NMuMG cells were cultured according to ATCC-recommended growth conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 24 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900070765,"sku":"SL100489-NMuMG-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901217645,"sku":"SL100489-NMuMG-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901250413,"sku":"SL100489-NMuMG-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901283181,"sku":"SL100489-NMuMG-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-NMUMG_8db30df5-33c8-495b-855e-d4527f6bc4c5.jpg?v=1790175948"},{"product_id":"genmute-sirna-transfection-reagent-for-smooth-muscle-cells-bht2200051","title":"GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePrimary and cultured smooth muscle cells take up siRNA efficiently with GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells (SMC), which comes pre-optimized for them. Residing in hollow-organ and blood vessel walls, smooth muscle cells carry essential roles in setting vascular tone, blood pressure, and organ contractility. Their low proliferation rate and their sensitivity to cytotoxic reagents nonetheless leave SMCs hard to transfect by conventional means.\u003c\/p\u003e\n\u003cp\u003eHigh uptake of siRNA together with minimal toxicity opens up gene silencing work spanning vascular biology, cardiovascular disease, airway function, and in vitro smooth muscle physiology.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eSmooth Muscle Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900103533,"sku":"SL100568-SMC-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905575789,"sku":"SL100568-SMC-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905608557,"sku":"SL100568-SMC-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905641325,"sku":"SL100568-SMC-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genmute-transfection-buffer-5x-bht2200057","title":"GenMute™ Transfection Buffer (5x)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eGenMute™ Transfection Buffer was developed for use with GenMute™ siRNA \u0026amp; DNA Transfection Reagent to give optimal transfection efficiency and maximum knockdown of gene expression. The buffer serves to dilute siRNA\/DNA and GenMute™ Transfection Reagent so that the most efficient transfection complex forms. Supplied as a 5x concentrated stock solution, GenMute™ Transfection Buffer (5x), 8.0 mL, makes 40 mL of working solution.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eBuffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at RT. If stored properly, the product is stable for 24 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eGenMute™ Transfection Buffer, 8.0 mL\/vial, to make 40 mL of working solution (1x ), sufficient for 1000 transfections in 24-well plate\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis transfection buffer is used to prepare transfection complexes with the matching SignaGen transfection reagent, for both DNA and siRNA delivery. Use it to dilute the nucleic acid and the reagent as described in the protocol.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"1.0 mL","offer_id":54042900037997,"sku":"SL100572-1P0ML","price":5.0,"currency_code":"USD","in_stock":true},{"title":"4.0 mL","offer_id":54042904756589,"sku":"SL100572-4P0ML","price":20.0,"currency_code":"USD","in_stock":true},{"title":"8.0 mL","offer_id":54042904789357,"sku":"SL100572-8P0ML","price":39.0,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mdck-cells-bht2200026","title":"GenJet™ In Vitro DNA Transfection Reagent for MDCK Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe MDCK (Madin–Darby Canine Kidney) line was started from kidney tissue of an adult female cocker spaniel that appeared normal, and it now serves as a standard model system in virology, epithelial cell biology, and membrane transport studies. Quite a range of viruses will infect these cells, vesicular stomatitis virus, vaccinia virus and Coxsackie viruses among them, along with selected reoviruses and adenoviruses. GenJet™ DNA In Vitro Transfection Reagent for MDCK Cells comes with its conditions tuned ahead of time, so plasmid DNA moves into MDCK cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMDCK\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300 transfections in 24-well plates or 150 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-MDCK.jpg?v=1790173498\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for MDCK Cells – GFP transfection in MDCK cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA Picture Showing the Transfection Efficiency of GenJet™ Reagent in MDCK Cells. MDCK cells were cultured according to ATCC-recommended conditions and transfected with the pEGFP-N3 plasmid using GenJet™ Reagent. Transfection efficiency was assessed 24 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900201837,"sku":"SL100489-MDCK-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902987117,"sku":"SL100489-MDCK-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903019885,"sku":"SL100489-MDCK-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903052653,"sku":"SL100489-MDCK-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-MDCK_fa67850f-635c-406d-bd7b-9e392bfbfb59.jpg?v=1790175944"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-d407-cells-bht2200014","title":"GenJet™ In Vitro DNA Transfection Reagent for D407 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eD407 is a human retinal pigment epithelial (RPE) cell line that laboratories commonly choose as an in vitro model for retinal biology, ocular diseases, gene regulation, and therapeutic gene delivery. Retinal pigment epithelial cells are critical to retinal homeostasis and photoreceptor function, so D407 gives researchers a valuable system for probing retinal physiology along with the molecular mechanisms behind retinal degeneration and other ocular disorders. Conditions in GenJet™ DNA In Vitro Transfection Reagent for D407 Cells are tuned to that line, so plasmid DNA transfection in D407 cells runs efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eD407\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900234605,"sku":"SL100489-D407-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901807469,"sku":"SL100489-D407-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901840237,"sku":"SL100489-D407-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901873005,"sku":"SL100489-D407-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}]},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-3t3-cells-bht2200006","title":"GenJet™ In Vitro DNA Transfection Reagent for 3T3 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eGeorge Todaro and Howard Green built the NIH 3T3 line out of Swiss mouse embryo tissue. This well-characterized mouse embryonic fibroblast line has since become one of the most-used fibroblast models across cell biology, molecular biology, and gene expression work — a standing it owes to robust growth characteristics together with reproducible biological responses. GenJet™ DNA In Vitro Transfection Reagent for NIH 3T3 Cells carries conditions tuned to that line specifically, so plasmid DNA enters NIH 3T3 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003e3T3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-3T3.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for 3T3 Cells – GFP transfection in NIH-3T3 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection efficiency of GenJet™ Reagent in NIH-3T3 cells. NIH-3T3 cells were cultured under ATCC-recommended conditions and transfected with the pEGFP-N3 expression plasmid using GenJet™ Reagent. GFP expression was assessed 24 hours post-transfection to evaluate transfection efficiency.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900267373,"sku":"SL100489-3T3-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901610861,"sku":"SL100489-3T3-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901643629,"sku":"SL100489-3T3-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901676397,"sku":"SL100489-3T3-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-3T3_dc12ec38-73a0-420e-93d5-bf58a93d18c6.jpg?v=1790175947"},{"product_id":"powerfect-in-vitro-sirna-transfection-reagent-ver-ii-bht2200054","title":"PowerFect™ In Vitro siRNA Transfection Reagent (Ver. II)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eBuilt on an enhanced liposome formulation, Powerfect™ siRNA Transfection Reagent targets in vitro RNA delivery at high efficiency.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e– Powerfect™ Reagent: Store at 4 °C. – Powerfect™ Transfection Buffer (5×): Store at room temperature. When stored properly, both components remain stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePowerfect™ siRNA Transfection Reagent (1.0 mL): Sufficient for approximately 1,330 transfections in 24-well plates using 5 pmol siRNA per well\u003c\/li\u003e\n\u003cli\u003ePowerfect™ Transfection Buffer (5×, 8.0 mL): Optimized for maximal transfection efficiency\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900300141,"sku":"SL100569-0P1ML","price":40.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905772397,"sku":"SL100569-0P5ML","price":196.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905805165,"sku":"SL100569-1P0ML","price":396.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905837933,"sku":"SL100569-1P0MLX5","price":1752.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PowerFect-Reagent-A-Liposome-Based-Transfection-Reagent_a72801be-22e8-4c1f-a957-1bc9e38661bb.png?v=1790175949"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-cv-1-cells-bht2200013","title":"GenJet™ In Vitro DNA Transfection Reagent for CV-1 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eCV-1, a fibroblast-like line, was established in 1964 from the kidney of an adult African green monkey (Chlorocebus aethiops, formerly Cercopithecus aethiops). Over the decades that followed it became a fixture of virology, molecular biology, and gene expression studies, and it is especially associated with simian virus 40 (SV40) research, with recombinant DNA technology, and with the building of transient gene expression systems. Adherent growth, vigorous proliferation, and ready susceptibility to a variety of viral infections make CV-1 cells a valuable model for studying viral replication, host–pathogen interactions, and transgene expression.\u003c\/p\u003e\n\u003cp\u003eGenJet™ DNA In Vitro Transfection Reagent for CV-1 Cells arrives tuned to this line, moving plasmid DNA into CV-1 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eCV-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-CV1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for CV-1 Cells – GFP transfection in CV-1 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative fluorescence image demonstrating the transfection efficiency of GenJet™ Reagent in CV-1 cells. CV-1 cells were cultured under ATCC-recommended growth conditions and transfected with the pEGFP-N3 reporter plasmid using GenJet™ Reagent. Transfection efficiency was evaluated 24 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900332909,"sku":"SL100489-CV1-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903675245,"sku":"SL100489-CV1-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903708013,"sku":"SL100489-CV1-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903740781,"sku":"SL100489-CV1-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-CV1_069a419f-c9e1-475d-ab1e-8becc81dbc3e.jpg?v=1790175948"},{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-epithelial-cells-bht2200015","title":"GenJet™ In Vitro DNA Transfection Reagent for Epithelial Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eA proprietary peptide ligand that targets epithelial cells is built into GenJet™ DNA In Vitro Transfection Reagent for Epithelial Cells, and it strengthens the reagent's interaction with epithelial cell surfaces. DNA complexes are consequently taken up better by the cells, and SignaGen reports transfection efficiency superior to that of conventional non-targeted formulations. Tuning covers a broad range of epithelial cell types, so plasmid DNA arrives efficiently, and the specialized design sustains robust gene expression without sacrificing high cell viability — a good fit for work on gene function, signal transduction, protein expression, and cellular engineering in epithelial cells.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eEpithelial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 600–1200 transfections in 24-well plates or 300–600 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_EPI.gif?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for Epithelial Cells – colonic epithelial cell GFP transfection vs other reagents\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of transfection efficiency in normal human colonic epithelial cells demonstrating the superior performance of the pre-optimized GenJet™ (Ver. II) Reagent. Transfections were carried out according to each manufacturer’s recommended protocol. Briefly, 0.5 µg of the GFP expression plasmid pEGFP-N3 was delivered per well in a 24-well plate using different transfection reagents. Transfection efficiency was evaluated 24 hours post-transfection by quantifying GFP-positive cells under a Nikon Eclipse 2000 fluorescence microscope.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900365677,"sku":"SL100489-EPI-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902692205,"sku":"SL100489-EPI-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902724973,"sku":"SL100489-EPI-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902757741,"sku":"SL100489-EPI-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_EPI_dd07ee3a-7d3e-4edd-9fd3-3240ae214ae2.gif?v=1790175947"},{"product_id":"genmute-sirna-transfection-reagent-for-c2c12-cells-bht2200039","title":"GenMute™ siRNA Transfection Reagent for C2C12 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eYaffe and Saxel (1977) built the C2C12 line by serially passaging, under selection, myoblasts that had been isolated from the thigh muscle of C3H mice after a crush injury. The resulting cells still proliferate as myoblasts and, given the right conditions, fuse into multinucleated myotubes — which is why they are a common in vitro model for skeletal muscle development, myogenesis, muscle metabolism, and signaling pathways, with myosin regulation and androgen receptor (AR) expression among the topics studied. GenMute™ siRNA Transfection Reagent for C2C12 Cells comes tuned ahead of time to move siRNA into C2C12 myoblasts efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eC2C12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of the cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900398445,"sku":"SL100568-C2C12-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905215341,"sku":"SL100568-C2C12-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905248109,"sku":"SL100568-C2C12-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905280877,"sku":"SL100568-C2C12-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/www.ebiohippo.com\/collections\/signagen-transfection-reagents.oembed","provider":"BioHippo","version":"1.0","type":"link"}