{"title":"StemImmune","description":"\u003cp\u003eAll StemImmune LLC products: human cell-expressed recombinant cytokines and growth factors for stem cell, hematopoiesis and immunology research.\u003c\/p\u003e","products":[{"product_id":"recombinant-human-activin-a-protein-bhp22200001","title":"Recombinant Human Activin A Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human Activin A Protein\u003c\/strong\u003e is a tag-free, disulfide-linked homodimer of the inhibin beta A subunit (gene \u003cem\u003eINHBA\u003c\/em\u003e), expressed in \u003cstrong\u003eHEK293 human cells\u003c\/strong\u003e. It is manufactured in an animal-free, xeno-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eActivin A is commonly used in human iPSC and ESC differentiation protocols, for primitive-streak and mesendoderm induction, definitive endoderm specification and the pancreatic and hepatic programs that follow. Expression in human cells supports mammalian folding and post-translational processing of the dimeric ligand. Each lot is tested for purity, endotoxin and biological activity before release, and the product has been reported in peer-reviewed studies on chemical reprogramming, pluripotent stem-cell culture and directed differentiation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: HEK293 human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eActivin A belongs to the TGF-β superfamily. It signals through heteromeric receptor complexes made of type II Activin receptors (mainly ACVR2A and ACVR2B) and the type I receptor ALK4 (ACVR1B). Receptor activation leads to phosphorylation of SMAD2 and SMAD3, which associate with SMAD4 and regulate context-dependent transcription in the nucleus.\u003c\/p\u003e\n\u003cp\u003eActivin signaling takes part in embryonic patterning, pluripotency, germ-layer specification, tissue morphogenesis, reproductive biology, inflammation, fibrosis, hematopoiesis and tissue repair. In pluripotent stem-cell work, the lineage that results is shaped by how much Activin A is added and for how long, by the starting cell state and medium, and by crosstalk with WNT, BMP, FGF and PI3K\/AKT signaling, so dose and timing need to be optimized for each cell line and target lineage.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eActivin A is mainly used in the early stages of directed differentiation of human pluripotent stem cells. The table summarizes where it is typically placed in a workflow; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of Activin A\u003c\/th\u003e\n\u003cth\u003eTypical stage\u003c\/th\u003e\n\u003cth\u003eOther signals often used\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDefinitive endoderm\u003c\/td\u003e\n\u003ctd\u003eDrives early endodermal programs\u003c\/td\u003e\n\u003ctd\u003eEarly differentiation, after pluripotent-cell preparation\u003c\/td\u003e\n\u003ctd\u003eWNT modulation; FGF\/PI3K control depending on the protocol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMesendoderm \/ primitive streak\u003c\/td\u003e\n\u003ctd\u003eSupports early germ-layer and primitive-streak-like transitions\u003c\/td\u003e\n\u003ctd\u003eVery early differentiation\u003c\/td\u003e\n\u003ctd\u003eWNT activation; BMP or FGF depending on the target lineage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePancreatic differentiation\u003c\/td\u003e\n\u003ctd\u003eEstablishes definitive endoderm\u003c\/td\u003e\n\u003ctd\u003eUpstream of pancreatic progenitor specification\u003c\/td\u003e\n\u003ctd\u003eRetinoid signaling, FGF, BMP modulation and pathway-specific small molecules\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHepatic differentiation\u003c\/td\u003e\n\u003ctd\u003eSupports early definitive-endoderm formation\u003c\/td\u003e\n\u003ctd\u003eBefore hepatic specification and maturation\u003c\/td\u003e\n\u003ctd\u003eBMP, FGF, HGF, OSM and maturation supplements\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoid research\u003c\/td\u003e\n\u003ctd\u003eSupports selected early developmental stages\u003c\/td\u003e\n\u003ctd\u003eDepends on organoid type and stage\u003c\/td\u003e\n\u003ctd\u003eWNT, BMP, FGF, EGF and tissue-specific signals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChemical reprogramming\u003c\/td\u003e\n\u003ctd\u003eSupports selected pluripotent-state or reprogramming conditions\u003c\/td\u003e\n\u003ctd\u003eProtocol-specific\u003c\/td\u003e\n\u003ctd\u003eDefined small-molecule combinations and other growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePluripotent stem-cell culture\u003c\/td\u003e\n\u003ctd\u003eActivin\/Nodal pathway support in some defined culture systems\u003c\/td\u003e\n\u003ctd\u003ePlatform-dependent; the requirement varies between systems\u003c\/td\u003e\n\u003ctd\u003eDefined basal medium and matrix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the stage Activin A is used for (pluripotency support, mesendoderm, definitive endoderm or a later step) and note the basal medium, WNT\/BMP\/FGF conditions and small molecules used with it.\u003c\/li\u003e\n\u003cli\u003eOptimize concentration and exposure time for each cell line. Published concentrations vary widely between applications, and exposure beyond the intended window can shift the resulting cell population.\u003c\/li\u003e\n\u003cli\u003eKey variables include starting-cell quality, seeding density at induction, medium and supplements, matrix or coating, and WNT, FGF, BMP and PI3K\/AKT activity.\u003c\/li\u003e\n\u003cli\u003eFor endoderm work, confirm the result with morphology and markers such as SOX17 and FOXA2, and check that the cells can progress to the intended downstream lineage.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eActivin A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eINHBA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHEK293 human cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eDisulfide-linked homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β superfamily\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene symbol\u003c\/th\u003e\n\u003ctd\u003eINHBA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt\u003c\/th\u003e\n\u003ctd\u003eP08476\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free manufacturing format\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 25 kDa. Refer to the lot-specific Certificate of Analysis (CoA) and SDS-PAGE profile for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized, carrier-protein free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInhibition of murine MPC-11 plasmacytoma cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEC50\u003c\/th\u003e\n\u003ctd\u003e6.68–10.02 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Stem Cell Culture, 3D cell culture, Cell Reprogramming, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by inhibition of murine MPC-11 plasmacytoma cell proliferation. The typical EC50 is 6.68–10.02 ng\/mL. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and avoid aggressive vortexing.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641413534061,"sku":"HST-A-0010","price":175.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641425265005,"sku":"HST-A-0100","price":1219.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/activina-500x500.webp?v=1791572710"},{"product_id":"recombinant-human-bmp-2-protein-bhp22200002","title":"Recombinant Human BMP-2 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human BMP-2 Protein\u003c\/strong\u003e is a full-length, non-glycosylated homodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and extremely low endotoxin (\u0026lt;0.5 EU\/µg).\u003c\/p\u003e\n\u003cp\u003eBMP-2 is a strong inducer of bone and cartilage formation. Researchers use it to drive mesenchymal stem cell (MSC) differentiation toward bone and cartilage, in tissue-engineering and biomaterial studies, in bone-repair research models and in work on bone disease mechanisms. Activity is confirmed by induction of alkaline phosphatase in ATDC-5 cells.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eBone morphogenetic protein 2 (BMP-2) belongs to the transforming growth factor β family, which includes more than 20 structurally related bone growth factors. Expression is highest in lung, spleen and colon; lower but significant levels occur in many other tissues, among them heart, brain, liver and kidney. Its main known role is the induction of bone and cartilage formation.\u003c\/p\u003e\n\u003cp\u003eMature human BMP-2 is usually a homodimer of two 114-amino-acid monomers. The two chains are joined by a disulfide bridge, and each monomer carries three intrachain disulfide bridges arranged in a cystine-knot motif.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eBMP-2 is used across bone and cartilage research, from stem-cell differentiation to biomaterial studies.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBone and cartilage formation: induction of osteogenic and chondrogenic programs in tissue-engineering and regenerative-biology research.\u003c\/li\u003e\n\u003cli\u003eStem cell differentiation: driving mesenchymal stem cells (MSCs) toward bone and cartilage lineages.\u003c\/li\u003e\n\u003cli\u003eBone repair models: research on bone healing and fracture repair.\u003c\/li\u003e\n\u003cli\u003eTissue engineering: incorporation into biomaterials to build experimental bone and cartilage grafts.\u003c\/li\u003e\n\u003cli\u003eDisease and target research: a tool for studying bone diseases and exploring potential therapeutic targets.\u003c\/li\u003e\n\u003cli\u003eBasic research: mechanisms of bone and cartilage development and regeneration.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eBMP-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eBone morphogenetic protein 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e30–38 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of alkaline phosphatase production in ATDC-5 mouse chondrogenic cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Differentiation Assay, Tissue Engineering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of BMP-2 to induce alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eChen D, Zhao M, Mundy GR (2004) Bone morphogenetic proteins. Growth Factors 22:233-241. \u003ca href=\"https:\/\/doi.org\/10.1080\/08977190412331279890\" target=\"_blank\" rel=\"noopener\"\u003e10.1080\/08977190412331279890\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWozney JM, Rosen V, Celeste AJ, et al. (1988) Novel regulators of bone formation: molecular clones and activities. Science 242:1528-1534. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.3201241\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.3201241\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641413828973,"sku":"HST-B2-0010","price":171.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641425690989,"sku":"HST-B2-0100","price":1197.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/BMP2-500x500.png?v=1791572717"},{"product_id":"recombinant-human-bmp-4-protein-bhp22200003","title":"Recombinant Human BMP-4 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human BMP-4 Protein\u003c\/strong\u003e is a tag-free, disulfide-linked homodimer of bone morphogenetic protein 4, expressed in \u003cstrong\u003eHEK293 human cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eBMP-4 is a standard tool for steering early lineage decisions in human iPSC and ESC differentiation: primitive-streak and mesoderm induction, followed by cardiovascular, endothelial, hemogenic-endothelium and hematopoietic programs. It is also used in organoid and developmental-biology models and in bone, cartilage and tissue-engineering research. Production in human cells supports mammalian folding and processing of the dimer, every lot is checked for purity, endotoxin and biological activity, and the protein has been reported in peer-reviewed work on chemical reprogramming, pluripotent stem-cell differentiation and hematopoietic development.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: HEK293 human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eBone Morphogenetic Protein 4 is a secreted member of the transforming growth factor beta (TGF-β) superfamily. BMPs were first described through their effects on bone formation, but BMP-4 acts much more widely, in embryonic patterning, germ-layer specification, tissue morphogenesis and cell-fate control.\u003c\/p\u003e\n\u003cp\u003eBMP-4 binds heteromeric complexes of type I and type II BMP receptors. Receptor activation phosphorylates the receptor-regulated SMAD1, SMAD5 and SMAD9, which pair with SMAD4 and move into the nucleus to control context-dependent gene expression. BMP-4 signaling can also engage non-SMAD pathways and cross-talk with WNT, Activin\/Nodal, FGF and VEGF networks.\u003c\/p\u003e\n\u003cp\u003eIn pluripotent stem-cell systems BMP-4 is usually added early to promote primitive-streak-like and mesodermal states; later pathway modulation then directs these cells toward cardiovascular, hematopoietic, endothelial, skeletal, hepatic or other fates. Because the outcome depends on concentration, timing, medium, cell state and companion signals, BMP-4 is best treated as a context-dependent developmental cue, with dose and exposure optimized for each cell line, basal medium and target lineage.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eBMP-4 is mainly used at the start of directed differentiation, where it sets up primitive-streak-like and mesodermal states that later signals turn into specific lineages. The table summarizes typical placements and the factors the manufacturer lists alongside BMP-4; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of BMP-4\u003c\/th\u003e\n\u003cth\u003eRepresentative factors\u003c\/th\u003e\n\u003cth\u003eExample outputs\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrimitive-streak induction\u003c\/td\u003e\n\u003ctd\u003eStarts primitive-streak-like programs in the first differentiation stage\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + Activin A + CHIR99021\u003c\/td\u003e\n\u003ctd\u003eMesoderm-competent intermediates from iPSCs\/ESCs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMesoderm specification\u003c\/td\u003e\n\u003ctd\u003eBuilds the mesoderm foundation for downstream lineages\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + VEGF + FGF2\u003c\/td\u003e\n\u003ctd\u003eCardiovascular, hematopoietic, MSC and skeletal precursors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCardiovascular differentiation\u003c\/td\u003e\n\u003ctd\u003eCardiac mesoderm induction and endothelial specification\u003c\/td\u003e\n\u003ctd\u003eVEGF165, FGF2, WNT modulation\u003c\/td\u003e\n\u003ctd\u003eCardiomyocytes, endothelial cells, vascular smooth muscle cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHemogenic endothelium\u003c\/td\u003e\n\u003ctd\u003eSupports mesoderm and hemogenic endothelium before endothelial-to-hematopoietic transition\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + VEGF + SCF + FLT3 Ligand\u003c\/td\u003e\n\u003ctd\u003eHemogenic endothelial populations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHSPC generation\u003c\/td\u003e\n\u003ctd\u003eEarly hematopoietic specification\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + SCF + TPO + FLT3 Ligand\u003c\/td\u003e\n\u003ctd\u003eHematopoietic progenitors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBone and cartilage\u003c\/td\u003e\n\u003ctd\u003eRegulates osteogenesis and chondrogenesis\u003c\/td\u003e\n\u003ctd\u003eBMP signaling, TGF-β family\u003c\/td\u003e\n\u003ctd\u003eMSC osteogenic and chondrogenic differentiation, bone and cartilage repair models\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoids and developmental models\u003c\/td\u003e\n\u003ctd\u003eEmbryonic patterning signal\u003c\/td\u003e\n\u003ctd\u003eStage-specific BMP, WNT, Activin and FGF\u003c\/td\u003e\n\u003ctd\u003eGastruloids, embryoid bodies, kidney, liver and heart organoids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTreat BMP-4 as a context-dependent signal: optimize concentration and exposure time for each cell line, basal medium and target lineage, starting from a validated protocol and a dose-response study.\u003c\/li\u003e\n\u003cli\u003eLineage outcome depends on the starting cell state, cytokine combination, timing and culture conditions; BMP-4 acts together with WNT, Activin\/Nodal, FGF, VEGF and Notch signaling.\u003c\/li\u003e\n\u003cli\u003eBMP-4 and the BMP antagonist Noggin can be used at different stages to switch BMP signaling on and later off in a controlled workflow.\u003c\/li\u003e\n\u003cli\u003eFor organoid work (kidney, liver, lung and others), set dose and timing according to the developmental logic of the specific system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eBMP-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eBone Morphogenetic Protein 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHEK293 human cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eDisulfide-linked homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β superfamily\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free manufacturing format\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized, carrier-protein free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of alkaline phosphatase activity in ATDC-5 mouse chondrogenic cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEC50\u003c\/th\u003e\n\u003ctd\u003eApproximately 5 ng\/mL (representative ATDC-5 assay)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, 3D cell culture, Cell Reprogramming, Tissue Engineering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eActivity is determined in ATDC-5 mouse chondrogenic cells, using alkaline phosphatase induction as the readout; in a representative assay the EC50 was approximately 5 ng\/mL. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently; do not vortex aggressively.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eShore EM, Xu M, Shah PB, et al. (1998) The human bone morphogenetic protein 4 (BMP-4) gene: molecular structure and transcriptional regulation. Calcif Tissue Int 63:221-229. \u003ca href=\"https:\/\/doi.org\/10.1007\/s002239900518\" target=\"_blank\" rel=\"noopener\"\u003e10.1007\/s002239900518\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGoldman DC, Hackenmiller R, Nakayama T, et al. (2006) Mutation of an upstream cleavage site in the BMP4 prodomain leads to tissue-specific loss of activity. Development 133:1933-1942. \u003ca href=\"https:\/\/doi.org\/10.1242\/dev.02368\" target=\"_blank\" rel=\"noopener\"\u003e10.1242\/dev.02368\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePerry JM, Harandi OF, Porayette P, et al. (2009) Maintenance of the BMP4-dependent stress erythropoiesis pathway in the murine spleen requires hedgehog signaling. Blood 113:911-918. \u003ca href=\"https:\/\/doi.org\/10.1182\/blood-2008-03-147892\" target=\"_blank\" rel=\"noopener\"\u003e10.1182\/blood-2008-03-147892\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eLian X, Hsiao C, Wilson G, et al. (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A 109:E1848-E1857. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.1200250109\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.1200250109\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641413927277,"sku":"HST-B4-0010","price":215.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641425887597,"sku":"HST-B4-0100","price":1536.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bmp4-500x500.webp?v=1791572721"},{"product_id":"recombinant-human-bmp-7-protein-bhp22200004","title":"Recombinant Human BMP-7 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human BMP-7 Protein\u003c\/strong\u003e (osteogenic protein 1, OP1) is a tag-free, full-length human BMP-7 expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a non-glycosylated homodimer. It is made in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eBMP-7 is used to study bone and cartilage formation, including differentiation of mesenchymal stem cells (MSCs) toward bone and cartilage, fracture-repair and tissue-engineering models, kidney development and bone disease. Its activity is confirmed by induction of alkaline phosphatase in the mouse chondrogenic cell line ATDC-5.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eBMP-7, also called osteogenic protein 1 (OP1), belongs to the transforming growth factor β (TGF-β) family, a group of more than 20 structurally related bone growth factors. It has an important role in cartilage and bone formation and also contributes to calcium regulation and kidney development.\u003c\/p\u003e\n\u003cp\u003eMature human BMP-7 is usually a homodimer of two antiparallel monomers of 139 amino acids. The monomers are linked by a disulfide bridge, and each one carries three intrachain disulfide bridges arranged in a cysteine-knot motif.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human BMP-7 is a research tool for bone, cartilage and stem-cell biology.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBone and cartilage formation studies, including tissue-engineering and regenerative-medicine models.\u003c\/li\u003e\n\u003cli\u003eDifferentiation of mesenchymal stem cells (MSCs) toward bone and cartilage.\u003c\/li\u003e\n\u003cli\u003eFracture-repair and bone-healing models.\u003c\/li\u003e\n\u003cli\u003eKidney development and function research.\u003c\/li\u003e\n\u003cli\u003eIncorporation into biomaterials to build bone and cartilage grafts for research.\u003c\/li\u003e\n\u003cli\u003eStudies of bone disease mechanisms and drug-target discovery.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eBMP-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eOP1 (osteogenic protein 1)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e30–38 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of alkaline phosphatase production in the ATDC-5 cell line (mouse chondrogenic cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Tissue Engineering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to induce alkaline phosphatase production in ATDC-5 cells, a mouse chondrogenic cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eWang RN, Green J, Wang Z, et al. (2014) Bone Morphogenetic Protein (BMP) signaling in development and human diseases. Genes Dis 1:87-105. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.gendis.2014.07.005\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.gendis.2014.07.005\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChen D, Zhao M, Mundy GR (2004) Bone morphogenetic proteins. Growth Factors 22:233-241. \u003ca href=\"https:\/\/doi.org\/10.1080\/08977190412331279890\" target=\"_blank\" rel=\"noopener\"\u003e10.1080\/08977190412331279890\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eCeleste AJ, Iannazzi JA, Taylor RC, et al. (1990) Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A 87:9843-9847. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.87.24.9843\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.87.24.9843\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641417531757,"sku":"HST-B7-0010","price":108.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426215277,"sku":"HST-B7-0100","price":769.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/BMP7-500x500.png?v=1791572728"},{"product_id":"recombinant-human-dc-cocktail-bhp22200005","title":"Recombinant Human DC Cocktail","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Cytokine Cocktail\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human DC Cocktail\u003c\/strong\u003e is a lyophilized supplement that combines recombinant human GM-CSF and IL-4, both expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e, at concentrations optimized for dendritic cell culture. It is tag-free, animal-free and xeno-free, lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eThe cocktail is formulated to drive differentiation of monocytes into dendritic cells (DCs). The resulting DCs serve immunology research on antigen presentation and T cell activation, DC-based vaccine development, cancer-immunotherapy models in which DCs present tumor antigens, and studies of DC involvement in autoimmune disease.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eDendritic cells are key antigen-presenting cells of the immune system and are central to studies of immune responses, antigen presentation and T cell activation. Because they present antigens very effectively, DCs are also used as a platform in vaccine and cancer-immunotherapy research.\u003c\/p\u003e\n\u003cp\u003eThis product supplies GM-CSF and IL-4 together in one vial. The concentrations of both cytokines have been optimized by the manufacturer for dendritic cell culture, and the reconstituted cocktail is added directly to dendritic cell basic medium.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eThe DC Cocktail is designed for generating dendritic cells from monocytes for immunology research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDifferentiation of monocytes into dendritic cells.\u003c\/li\u003e\n\u003cli\u003eImmunology research on immune responses, antigen presentation and T cell activation.\u003c\/li\u003e\n\u003cli\u003eDC-based vaccine development, using DCs as antigen-presenting platforms.\u003c\/li\u003e\n\u003cli\u003eCancer-immunotherapy models in which DCs present tumor antigens to stimulate anti-tumor responses.\u003c\/li\u003e\n\u003cli\u003eStudies of the role of DCs in autoimmune disease.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eGM-CSF, IL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity confirmation\u003c\/th\u003e\n\u003ctd\u003eSDS-PAGE with Coomassie blue staining\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation (human erythroleukemic indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eComponents\u003c\/th\u003e\n\u003ctd\u003eRecombinant human GM-CSF and recombinant human IL-4, at concentrations optimized for dendritic cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Differentiation Assay, Antigen Presentation Assay, T Cell Activation Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the cocktail to stimulate proliferation of human TF-1 cells, an erythroleukemic indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute the contents in 200 µL sterile PBS or basic medium.\u003c\/li\u003e\n\u003cli\u003eAdd the reconstituted cocktail to an appropriate volume of dendritic cell basic medium.\u003c\/li\u003e\n\u003cli\u003eMedium supplemented with the DC cocktail can be stored at 4°C for one month.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"1 vial (for 100 mL media)","offer_id":67641417597293,"sku":"HCT-G4-0100","price":201.0,"currency_code":"USD","in_stock":true},{"title":"1 vial (for 500 mL media)","offer_id":67641426379117,"sku":"HCT-G4-0500","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DC-500x500.png?v=1791572732"},{"product_id":"heat-stable-recombinant-human-fgf-basic-protein-bhp22200006","title":"Heat-Stable Recombinant Human FGF-basic Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHeat-Stable Recombinant Human FGF-basic Protein\u003c\/strong\u003e (FGF2, bFGF) is a low-molecular-weight, monomeric FGF2 expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is designed to give more stable FGF2 signaling during extended cell culture, and is supplied tag-free, animal-free and xeno-free, lyophilized from a 0.2 µm-filtered PBS solution without carrier protein.\u003c\/p\u003e\n\u003cp\u003ePurity is \u0026gt;95% by SDS-PAGE and endotoxin is \u0026lt;0.5 EU\/µg. Typical research uses include human iPSC\/ESC maintenance and expansion, FGF-responsive progenitor expansion, organoid culture and FGFR signaling studies.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Heat-stable · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eFGF2 binds heparin and acts through fibroblast growth factor receptors (FGFRs), with cell-surface heparan sulfate as a co-receptor. The common low-molecular-weight human form has 154 amino acids and binds several FGFR splice isoforms, including FGFR1c, FGFR2c, FGFR3c and FGFR4. Downstream pathways include RAS–MAPK\/ERK, PI3K–AKT and PLCγ.\u003c\/p\u003e\n\u003cp\u003eNative FGF2 is sensitive to prolonged incubation under cell-culture conditions, so stabilized FGF2 variants were developed to keep signaling more constant between medium changes. \"Heat-stable\" refers to this culture stability; it does not mean the protein is validated for sterilization, heat processing or therapeutic use. Stabilized FGF2 variants are not interchangeable at the same dose, so the concentration should be optimized for each cell line, medium and matrix.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eFGF2 is used wherever cells depend on FGF signaling for self-renewal, expansion or survival, and is also a common ligand for FGFR–ERK and PI3K–AKT signaling studies. A heat-stable form may help keep available FGF2 more even between feeds in longer culture workflows.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of FGF2\u003c\/th\u003e\n\u003cth\u003eCompanion signals and conditions\u003c\/th\u003e\n\u003cth\u003eCommon readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eiPSC\/ESC maintenance\u003c\/td\u003e\n\u003ctd\u003eSelf-renewal, proliferation and survival\u003c\/td\u003e\n\u003ctd\u003eTGF-β\/Activin support, defined matrix and basal medium\u003c\/td\u003e\n\u003ctd\u003eOCT4, SOX2, NANOG, morphology, growth rate, karyotype\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeural progenitor expansion\u003c\/td\u003e\n\u003ctd\u003eMitogenic and survival signal\u003c\/td\u003e\n\u003ctd\u003eEGF and stage-specific pathway modulation\u003c\/td\u003e\n\u003ctd\u003eSOX1, PAX6, NESTIN; expansion and differentiation capacity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMesenchymal \/ stromal culture\u003c\/td\u003e\n\u003ctd\u003eProliferation and phenotype support\u003c\/td\u003e\n\u003ctd\u003eSerum-free or defined basal medium; matrix-dependent\u003c\/td\u003e\n\u003ctd\u003eGrowth kinetics, morphology, identity markers, functional assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndothelial research\u003c\/td\u003e\n\u003ctd\u003eProliferation and angiogenic signaling\u003c\/td\u003e\n\u003ctd\u003eVEGF-165, extracellular matrix, flow or mechanical cues\u003c\/td\u003e\n\u003ctd\u003eCD31, VE-cadherin, KDR; migration, tube or sprouting assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoid culture\u003c\/td\u003e\n\u003ctd\u003eStage-specific expansion or patterning\u003c\/td\u003e\n\u003ctd\u003eEGF, WNT, BMP\/Noggin, Activin and tissue-specific signals\u003c\/td\u003e\n\u003ctd\u003eOrganoid-forming efficiency, size, lineage markers, function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDecide the role of FGF2 in the experiment (maintenance, expansion, survival, patterning or a signaling assay), start from a validated concentration, then run a dose response in the chosen cell line.\u003c\/li\u003e\n\u003cli\u003eValidate any change in feeding interval in the complete medium. \"Heat-stable\" alone does not establish a reduced or weekend-free feeding schedule.\u003c\/li\u003e\n\u003cli\u003eHeparin, heparan sulfate, matrix and other proteoglycans affect FGF2 stability and FGFR signaling, so keep these conditions consistent between experiments.\u003c\/li\u003e\n\u003cli\u003eWhen replacing a conventional FGF2, compare equivalent molar concentrations and check both short-term signaling and long-term phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eFGF-basic (heat-stable FGF2; also known as FGF2 \/ bFGF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eFGF2; bFGF; FGF-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein form\u003c\/th\u003e\n\u003ctd\u003eLow-molecular-weight FGF2; monomeric recombinant protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene symbol\u003c\/th\u003e\n\u003ctd\u003eFGF2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free, xeno-free and tag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 17 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of 3T3 mouse fibroblast cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Culture, Cell Proliferation, 3D cell culture, Angiogenesis Assay, Cell Signaling Studies, Stem Cell Differentiation, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of 3T3 mouse fibroblast cells. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently; do not vortex aggressively.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eOrnitz DM, Xu J, Colvin JS, et al. (1996) Receptor specificity of the fibroblast growth factor family. J Biol Chem 271:15292-15297. \u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.271.25.15292\" target=\"_blank\" rel=\"noopener\"\u003e10.1074\/jbc.271.25.15292\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChen G, Gulbranson DR, Yu P, et al. (2012) Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30:623-630. \u003ca href=\"https:\/\/doi.org\/10.1002\/stem.1021\" target=\"_blank\" rel=\"noopener\"\u003e10.1002\/stem.1021\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eOnuma Y, Higuchi K, Aiki Y, et al. (2015) A stable chimeric fibroblast growth factor (FGF) can successfully replace basic FGF in human pluripotent stem cell culture. PLoS One 10:e0118931. \u003ca href=\"https:\/\/doi.org\/10.1371\/journal.pone.0118931\" target=\"_blank\" rel=\"noopener\"\u003e10.1371\/journal.pone.0118931\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKoledova Z, Sumbal J, Rabata A, et al. (2019) Fibroblast Growth Factor 2 Protein Stability Provides Decreased Dependence on Heparin for Induction of FGFR Signaling and Alters ERK Signaling Dynamics. Front Cell Dev Biol 7:331. \u003ca href=\"https:\/\/doi.org\/10.3389\/fcell.2019.00331\" target=\"_blank\" rel=\"noopener\"\u003e10.3389\/fcell.2019.00331\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKim S, Kang GH, Lim KM, et al. (2023) Thermostable Human Basic Fibroblast Growth Factor (TS-bFGF) Engineered with a Disulfide Bond Demonstrates Superior Culture Outcomes in Human Pluripotent Stem Cell. Biology (Basel) 12:888. \u003ca href=\"https:\/\/doi.org\/10.3390\/biology12060888\" target=\"_blank\" rel=\"noopener\"\u003e10.3390\/biology12060888\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKuo HH, Gao X, DeKeyser JM, et al. (2020) Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports 14:256-270. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.stemcr.2019.12.007\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.stemcr.2019.12.007\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641418219885,"sku":"HST-HS2-0010","price":90.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426542957,"sku":"HST-HS2-0100","price":628.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hs_f2-500x500.webp?v=1791572736"},{"product_id":"recombinant-human-fgf-7-kgf-protein-bhp22200007","title":"Recombinant Human FGF-7 (KGF) Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human FGF-7 (KGF) Protein\u003c\/strong\u003e is keratinocyte growth factor expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated homodimer. It is tag-free, animal-free and xeno-free, and is lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eFGF-7 is an epithelium-selective growth factor that acts through FGFR2b. Typical research uses are keratinocyte and epithelial proliferation, organoid culture and staged endoderm differentiation, branching morphogenesis and epithelial repair models. Expression in human cells supports mammalian processing of the protein, and three peer-reviewed studies report use of this FGF-7.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eFGF-7, also called keratinocyte growth factor (KGF), is a fibroblast growth factor made mainly by mesenchymal cells. It acts as a paracrine signal on neighboring epithelial cells, primarily through the FGFR2b receptor isoform, and activates MAPK, PI3K–AKT and related pathways that support proliferation and survival.\u003c\/p\u003e\n\u003cp\u003eFGF-7 research spans keratinocyte biology and epithelial repair, branching morphogenesis, development of the lung and pancreas, and organoid and staged endoderm differentiation models. The response depends on FGFR2b expression, developmental stage, matrix and companion signals.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eFGF-7 is used wherever FGFR2b-positive epithelial cells need a paracrine growth or survival signal. The table shows typical placements; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of FGF-7\u003c\/th\u003e\n\u003cth\u003eCompanion context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEpithelial proliferation\u003c\/td\u003e\n\u003ctd\u003eDirect FGFR2b stimulation\u003c\/td\u003e\n\u003ctd\u003eDefined serum context\u003c\/td\u003e\n\u003ctd\u003eCell count, EdU\/Ki-67, pERK and viability\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePancreatic \/ endoderm differentiation\u003c\/td\u003e\n\u003ctd\u003eStage-specific support of epithelial progenitors\u003c\/td\u003e\n\u003ctd\u003eActivin A, retinoic acid and other protocol factors\u003c\/td\u003e\n\u003ctd\u003ePDX1, NKX6.1, organoid morphology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBranching morphogenesis\u003c\/td\u003e\n\u003ctd\u003eParacrine epithelial growth signal\u003c\/td\u003e\n\u003ctd\u003eMatrix, with FGF10\/EGF controls\u003c\/td\u003e\n\u003ctd\u003eBranch number, epithelial markers and FGFR2b\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRepair models\u003c\/td\u003e\n\u003ctd\u003eEpithelial survival and proliferation\u003c\/td\u003e\n\u003ctd\u003eInjury model and time course\u003c\/td\u003e\n\u003ctd\u003eClosure, apoptosis, proliferation and barrier function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the responsive cell stage and confirm FGFR2b expression or pathway competence before adding FGF-7.\u003c\/li\u003e\n\u003cli\u003eBegin with conditions from a validated protocol and titrate FGF-7 in the actual cells, medium and endpoint; no numerical EC50 is given for this product.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors such as EGF, FGF-10 or Activin A, since signal order and duration can change the outcome.\u003c\/li\u003e\n\u003cli\u003eInclude no-factor, vehicle and pathway-response controls, and measure phenotype and function rather than cell number alone.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eFGF-7 (also known as KGF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eKGF; keratinocyte growth factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eFibroblast growth factor (FGF) family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ Xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e17–30 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of 4MBr-5 monkey epithelial cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Proliferation, 3D cell culture, Cell Migration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by stimulation of 4MBr-5 monkey epithelial cell proliferation.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare aliquots.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eFinch PW, Rubin JS, Miki T, et al. (1989) Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth. Science 245:752-755. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.2475908\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.2475908\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinch PW, Rubin JS (2004) Keratinocyte growth factor\/fibroblast growth factor 7, a homeostatic factor with therapeutic potential for epithelial protection and repair. Adv Cancer Res 91:69-136. \u003ca href=\"https:\/\/doi.org\/10.1016\/S0065-230X(04)91003-2\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/S0065-230X(04)91003-2\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641418285421,"sku":"HST-F7-0010","price":112.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426674029,"sku":"HST-F7-0100","price":779.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FGF7-500x500.png?v=1791572743"},{"product_id":"recombinant-human-vegf-121-protein-bhp22200008","title":"Recombinant Human VEGF-121 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human VEGF-121 Protein\u003c\/strong\u003e is a tag-free, full-length human VEGF-121 expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated homodimer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eVEGF-121 is a research tool for angiogenesis and vascular biology, including blood-vessel formation, wound-healing and tissue-engineering models that need a vascular network, and tumor angiogenesis in cancer research. Its activity is confirmed by stimulation of HUVEC proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eVascular endothelial growth factor (VEGF) is a potent growth and angiogenic cytokine of the PDGF family, defined by eight conserved cysteine residues and a cystine-knot structure. VEGF drives angiogenesis, vasculogenesis and endothelial cell growth: it induces endothelial cell proliferation, promotes migration, inhibits apoptosis and increases blood-vessel permeability.\u003c\/p\u003e\n\u003cp\u003eHuman cells express several alternatively spliced VEGF isoforms. VEGF-121 is the only isoform without the basic heparin-binding region, so it is freely diffusible. It also induces proliferation of lymphatic endothelial cells, and up-regulation of VEGF-121 may promote lymphangiogenesis.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human VEGF-121 is used across angiogenesis, vascular-biology and cancer research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAngiogenesis studies of blood-vessel formation.\u003c\/li\u003e\n\u003cli\u003eWound-healing models that depend on blood-vessel growth and tissue repair.\u003c\/li\u003e\n\u003cli\u003eTissue-engineering work aimed at building a robust vascular network.\u003c\/li\u003e\n\u003cli\u003eTumor-angiogenesis research in cancer biology.\u003c\/li\u003e\n\u003cli\u003eDrug-discovery studies related to ischemic disease and tissue regeneration.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eVEGF-121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003ePDGF family (VEGF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e37 kDa homodimer, 50 kDa homotrimer. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of HUVEC (human umbilical vein endothelial cell) proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Angiogenesis Assay, Cell Proliferation, Tissue Engineering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of HUVECs (human umbilical vein endothelial cells).\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eLeung DW, Cachianes G, Kuang WJ, et al. (1989) Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306-1309. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.2479986\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.2479986\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRobinson CJ, Stringer SE (2001) The splice variants of vascular endothelial growth factor (VEGF) and their receptors. J Cell Sci 114:853-865. \u003ca href=\"https:\/\/doi.org\/10.1242\/jcs.114.5.853\" target=\"_blank\" rel=\"noopener\"\u003e10.1242\/jcs.114.5.853\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eTischer E, Mitchell R, Hartman T, et al. (1991) The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J Biol Chem 266:11947-11954. \u003ca href=\"https:\/\/doi.org\/10.1016\/S0021-9258(18)99049-6\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/S0021-9258(18)99049-6\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eByrne AM, Bouchier-Hayes DJ, Harmey JH (2005) Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J Cell Mol Med 9:777-794. \u003ca href=\"https:\/\/doi.org\/10.1111\/j.1582-4934.2005.tb00379.x\" target=\"_blank\" rel=\"noopener\"\u003e10.1111\/j.1582-4934.2005.tb00379.x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641418744173,"sku":"HVG-VF1-0010","price":101.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426739565,"sku":"HVG-VF1-0100","price":808.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VEGF121-500x500.png?v=1791572750"},{"product_id":"recombinant-human-vegf-165-protein-bhp22200009","title":"Recombinant Human VEGF-165 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human VEGF-165 Protein\u003c\/strong\u003e (VEGF-A165) is made in \u003cstrong\u003ehuman cells\u003c\/strong\u003e; each molecule is a glycosylated homodimer of two 165-amino-acid chains joined by disulfide bonds. It is tag-free, animal-free and xeno-free, lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eVEGF-165 is used for endothelial-cell culture, HUVEC proliferation and angiogenesis assays, endothelial and hemogenic-endothelium differentiation of human iPSCs and ESCs, vascular organoids, tissue engineering and tumor-angiogenesis research. Activity is confirmed by HUVEC proliferation with an EC50 of 7.6–11.4 ng\/mL, every lot is quality-control tested, and the protein has been reported in eight peer-reviewed studies.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eVascular Endothelial Growth Factor A (VEGF-A), also called vascular permeability factor, is a key regulator of blood-vessel development and endothelial-cell function. It belongs to the platelet-derived growth factor family and carries the conserved cystine-knot structure of that family. Alternative splicing of the human VEGFA gene produces several isoforms; VEGF-165 is the predominant isoform and one of the most biologically active, combining receptor binding with extracellular-matrix and neuropilin interactions.\u003c\/p\u003e\n\u003cp\u003eThe main receptor for VEGF-165 is VEGFR-2\/KDR; VEGFR-1\/Flt-1 and co-receptors such as neuropilin-1 also contribute. It promotes endothelial proliferation, migration and survival, vascular permeability, angiogenesis and vasculogenesis. The response depends on cell state, receptor expression, concentration, exposure time, matrix and accompanying developmental signals, so dose and timing should be optimized for each cell line, basal medium and objective.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eVEGF-165 is used across endothelial culture, angiogenesis assays and staged hPSC differentiation, where it is usually added after mesoderm induction. The table summarizes typical uses with the starting ranges the manufacturer suggests; ranges are starting points for optimization, not universal protocols.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of VEGF-165\u003c\/th\u003e\n\u003cth\u003eExample models and readouts\u003c\/th\u003e\n\u003cth\u003eSuggested starting range\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndothelial-cell culture\u003c\/td\u003e\n\u003ctd\u003eProliferation, survival and VEGFR-2\/KDR signaling\u003c\/td\u003e\n\u003ctd\u003eHUVEC and primary endothelial cells, endothelial progenitor expansion\u003c\/td\u003e\n\u003ctd\u003e5–25 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAngiogenesis and vasculogenesis assays\u003c\/td\u003e\n\u003ctd\u003eMigration, sprouting and network formation\u003c\/td\u003e\n\u003ctd\u003eTube-formation, spheroid-sprouting, migration and wound-closure assays\u003c\/td\u003e\n\u003ctd\u003e10–50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ehPSC endothelial differentiation\u003c\/td\u003e\n\u003ctd\u003eVascular-progenitor and endothelial specification after mesoderm induction\u003c\/td\u003e\n\u003ctd\u003eVEGFR-2\/KDR-positive progenitors, CD31+\/CD144+ endothelial cells\u003c\/td\u003e\n\u003ctd\u003e25–100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHemogenic endothelium\u003c\/td\u003e\n\u003ctd\u003eVascular specification before endothelial-to-hematopoietic transition\u003c\/td\u003e\n\u003ctd\u003eCD34+\/CD144+ hemogenic endothelium, early HSPCs\u003c\/td\u003e\n\u003ctd\u003e25–50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVascular organoids and 3D models\u003c\/td\u003e\n\u003ctd\u003eEndothelial specification and vessel-like network formation\u003c\/td\u003e\n\u003ctd\u003eVascular organoids, hydrogels, organoid vascularization\u003c\/td\u003e\n\u003ctd\u003e25–100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTissue engineering\u003c\/td\u003e\n\u003ctd\u003eVascularization of engineered tissues\u003c\/td\u003e\n\u003ctd\u003eScaffolds, hydrogels, endothelial co-cultures, wound-healing models\u003c\/td\u003e\n\u003ctd\u003eNot specified; optimize per system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTumor angiogenesis\u003c\/td\u003e\n\u003ctd\u003eVEGF-dependent vascular responses in the tumor microenvironment\u003c\/td\u003e\n\u003ctd\u003eVEGFR-2 phosphorylation, ERK\/MAPK and PI3K\/AKT, permeability\u003c\/td\u003e\n\u003ctd\u003eNot specified; optimize per system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTreat the suggested ranges as starting points; to repeat a published method, use the concentration, timing, basal medium and companion factors reported there.\u003c\/li\u003e\n\u003cli\u003eIn hPSC differentiation the timing of VEGF exposure matters as much as the concentration, because responsiveness changes with developmental stage.\u003c\/li\u003e\n\u003cli\u003eCompanion factors depend on stage and lineage and may include BMP-4, Activin A, FGF-basic\/FGF2, EGF, SCF, FLT3 Ligand, TPO, IL-3 and IL-6.\u003c\/li\u003e\n\u003cli\u003eOutcome also depends on cell state, extracellular matrix and culture conditions, so keep these constant when comparing conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eVEGF-165\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eVEGF-A165\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated, disulfide-linked homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003ePDGF family (cystine-knot growth factor)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene symbol\u003c\/th\u003e\n\u003ctd\u003eVEGFA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-Free \/ Xeno-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 39–45 kDa as a glycosylated homodimer. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of HUVEC proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEC50\u003c\/th\u003e\n\u003ctd\u003e7.6–11.4 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSuggested concentration\u003c\/th\u003e\n\u003ctd\u003eEndothelial-cell culture: 5–25 ng\/mL; migration or tube-formation assays: 10–50 ng\/mL; hPSC endothelial differentiation: 25–100 ng\/mL; hemogenic-endothelium differentiation: 25–50 ng\/mL; vascular organoid or 3D culture: 25–100 ng\/mL (starting points for optimization)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Angiogenesis Assay, Cell Proliferation, Cell Migration, Hematopoietic Differentiation, 3D cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eActivity is determined as HUVEC proliferation in response to VEGF-165, giving an EC50 of 7.6–11.4 ng\/mL in the manufacturer's assay; the EC50 can shift with cell type, culture conditions, assay format and protocol. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently until dissolved; do not vortex vigorously.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots and avoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003cli\u003eChoose the final working concentration according to cell type, culture system, application and protocol.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eLeung DW, Cachianes G, Kuang WJ, et al. (1989) Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306-1309. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.2479986\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.2479986\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eTischer E, Mitchell R, Hartman T, et al. (1991) The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J Biol Chem 266:11947-11954. \u003ca href=\"https:\/\/doi.org\/10.1016\/S0021-9258(18)99049-6\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/S0021-9258(18)99049-6\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNeufeld G, Cohen T, Gengrinovitch S, et al. (1999) Vascular endothelial growth factor (VEGF) and its receptors. FASEB J 13:9-22. \u003ca href=\"https:\/\/doi.org\/10.1096\/fasebj.13.1.9\" target=\"_blank\" rel=\"noopener\"\u003e10.1096\/fasebj.13.1.9\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eByrne AM, Bouchier-Hayes DJ, Harmey JH (2005) Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J Cell Mol Med 9:777-794. \u003ca href=\"https:\/\/doi.org\/10.1111\/j.1582-4934.2005.tb00379.x\" target=\"_blank\" rel=\"noopener\"\u003e10.1111\/j.1582-4934.2005.tb00379.x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641418940781,"sku":"HVG-VF5-0010","price":150.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641427001709,"sku":"HVG-VF5-0100","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VEGF165-500x500.png?v=1791572754"},{"product_id":"recombinant-human-flt3-ligand-protein-bhp22200010","title":"Recombinant Human FLT3 Ligand Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Cytokine\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human FLT3 Ligand Protein\u003c\/strong\u003e (FLT3L) is a hematopoietic cytokine expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated monomer. It is tag-free, animal-free and xeno-free, and is lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eFLT3 Ligand is used in hematopoietic stem\/progenitor-cell culture, usually together with SCF and TPO, in dendritic-cell development and in staged differentiation of immune cells from pluripotent stem cells, as well as in FLT3\/CD135 signaling studies. Expression in human cells supports mammalian folding and glycosylation, and five peer-reviewed studies identify this FLT3 Ligand or its catalog number.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eFLT3 Ligand is a glycoprotein cytokine produced by several stromal and immune-cell populations. The soluble form is released from a membrane-associated precursor and binds the receptor tyrosine kinase FLT3\/CD135 on early hematopoietic progenitors.\u003c\/p\u003e\n\u003cp\u003eReceptor activation engages PI3K–AKT, RAS–MAPK and STAT signaling, and the result depends on progenitor state and companion cytokines. FLT3L acts most clearly on early progenitors and dendritic-cell lineages, and is studied with SCF and TPO in HSPC culture and with stage-specific signals in dendritic, lymphoid and other immune-cell differentiation.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eFLT3 Ligand is mostly used in combination with other cytokines to support FLT3-positive early progenitors and dendritic-cell lineages. The table shows typical placements; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of FLT3 Ligand\u003c\/th\u003e\n\u003cth\u003eCompanion context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCord-blood or adult CD34+ HSPC culture\u003c\/td\u003e\n\u003ctd\u003eSurvival and expansion of early progenitors\u003c\/td\u003e\n\u003ctd\u003eSCF and TPO, plus protocol-dependent signals\u003c\/td\u003e\n\u003ctd\u003eCell count, viability, CD34\/CD38\/CD90, CFU and engraftment assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ehPSC hematopoietic differentiation\u003c\/td\u003e\n\u003ctd\u003eSupport after hemogenic\/endothelial stages\u003c\/td\u003e\n\u003ctd\u003eSCF, TPO, IL-3, IL-7 and\/or Notch context\u003c\/td\u003e\n\u003ctd\u003eCD43, CD45, CD34, lineage markers and functional assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDendritic-cell research\u003c\/td\u003e\n\u003ctd\u003eExpansion of FLT3-responsive progenitors\u003c\/td\u003e\n\u003ctd\u003eLineage-specific maturation signals\u003c\/td\u003e\n\u003ctd\u003eDC subset markers, antigen presentation and cytokine output\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFLT3 signaling assay\u003c\/td\u003e\n\u003ctd\u003eReceptor stimulation\u003c\/td\u003e\n\u003ctd\u003eDefined serum-reduced or basal system\u003c\/td\u003e\n\u003ctd\u003ePhospho-FLT3, ERK, AKT, STAT5 and proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the developmental or immune-cell stage and confirm that the target population expresses FLT3\/CD135 or depends on the pathway.\u003c\/li\u003e\n\u003cli\u003eTake the starting dose from published or in-house qualified work and titrate it for each cell source, basal medium and readout; no universal working concentration or EC50 applies across systems.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion cytokines, since signal order, duration and pathway interactions shape the outcome.\u003c\/li\u003e\n\u003cli\u003eInclude no-factor, vehicle and pathway-response controls, and assess identity, viability, signaling and function rather than cell number alone.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eFLT3 Ligand\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eFLT3L; Flt3 Ligand; FLT3-Ligand\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eHematopoietic cytokine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ Xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e24–30 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eProliferation of human OCI-AML5 cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Cell Proliferation, Colony-Forming Unit Assay, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is shown by dose-dependent stimulation of proliferation in human OCI-AML5 cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eHannum C, Culpepper J, Campbell D, et al. (1994) Ligand for FLT3\/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature 368:643-648. \u003ca href=\"https:\/\/doi.org\/10.1038\/368643a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/368643a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSavvides SN, Boone T, Andrew Karplus P (2000) Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots. Nat Struct Biol 7:486-491. \u003ca href=\"https:\/\/doi.org\/10.1038\/75896\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/75896\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419202925,"sku":"HHM-FT-0010","price":101.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641427034477,"sku":"HHM-FT-0100","price":808.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FLT3ligand1-500x500.png?v=1791572762"},{"product_id":"recombinant-human-epo-protein-bhp22200011","title":"Recombinant Human EPO Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Glycoprotein Hormone\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human EPO Protein\u003c\/strong\u003e (erythropoietin) is expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated monomer. It is tag-free, animal-free and xeno-free, and is lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eEPO is used to support survival and maturation of EPOR-responsive erythroid progenitors, to study stage-specific erythroid commitment from CD34+ or pluripotent-cell-derived progenitors, to measure JAK2\/STAT5, PI3K–AKT and MAPK responses, and in TF-1 proliferation and comparative potency assays. Expression in human cells supports mammalian glycosylation of the protein.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eErythropoietin is a glycoprotein hormone made mainly by the kidney when oxygen is low. On erythroid progenitors it binds the erythropoietin receptor (EPOR); downstream of EPOR\/JAK2, the STAT5, PI3K–AKT and MAPK pathways promote cell survival, proliferation and differentiation.\u003c\/p\u003e\n\u003cp\u003eEPO acts mainly on committed erythroid progenitors, especially CFU-E and early erythroblasts, while SCF and other signals act at earlier stages. In the laboratory it is used to study erythropoiesis, erythroid progenitor biology, hemoglobinization and EPOR signaling.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eEPO is used mainly at committed erythroid stages and in EPOR signaling and potency assays. The table shows typical placements; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of EPO\u003c\/th\u003e\n\u003cth\u003eCompanion context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCD34+ erythroid differentiation\u003c\/td\u003e\n\u003ctd\u003eSupport of committed erythroid stages\u003c\/td\u003e\n\u003ctd\u003eSCF and stage-specific cytokine withdrawal\u003c\/td\u003e\n\u003ctd\u003eCD71, glycophorin A, hemoglobinization and enucleation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ehPSC-derived erythropoiesis\u003c\/td\u003e\n\u003ctd\u003eStage-specific erythroid maturation\u003c\/td\u003e\n\u003ctd\u003eMesoderm and hematopoietic induction factors at earlier stages\u003c\/td\u003e\n\u003ctd\u003eCD43, CD45, CD235a, globin expression\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEPOR signaling assay\u003c\/td\u003e\n\u003ctd\u003eAcute receptor activation\u003c\/td\u003e\n\u003ctd\u003eSerum-reduced conditions and time course\u003c\/td\u003e\n\u003ctd\u003epJAK2, pSTAT5, pAKT and target genes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTF-1 potency assay\u003c\/td\u003e\n\u003ctd\u003eDose-dependent proliferation\u003c\/td\u003e\n\u003ctd\u003eDefined assay medium\u003c\/td\u003e\n\u003ctd\u003eProliferation, viability and relative potency\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the responsive cell stage and confirm EPOR expression or pathway competence.\u003c\/li\u003e\n\u003cli\u003eBegin with conditions from a validated protocol and titrate EPO in the actual cells, medium and endpoint; no numerical EC50 or universal working concentration is given for this product.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors such as SCF, IL-3 and TPO, since signal order and duration can change lineage or functional outcomes.\u003c\/li\u003e\n\u003cli\u003eInclude no-factor, vehicle and pathway-response controls, and measure phenotype and function rather than cell number alone.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eEPO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eErythropoietin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eGlycoprotein hormone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ Xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e36 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Cell Proliferation, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by stimulation of human TF-1 cell proliferation, with a concentration-dependent response.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare aliquots.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eJelkmann W, et al. (2007) Erythropoietin after a century of research: younger than ever. Eur J Haematol 78:183-205. \u003ca href=\"https:\/\/doi.org\/10.1111\/j.1600-0609.2007.00818.x\" target=\"_blank\" rel=\"noopener\"\u003e10.1111\/j.1600-0609.2007.00818.x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eXavier-Ferrucio J, et al. (2018) Concise Review: Bipotent Megakaryocytic-Erythroid Progenitors: Concepts and Controversies. Stem Cells 36:1138-1145. \u003ca href=\"https:\/\/doi.org\/10.1002\/stem.2834\" target=\"_blank\" rel=\"noopener\"\u003e10.1002\/stem.2834\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eDulmovits BM, et al. (2017) Characterization, regulation, and targeting of erythroid progenitors in normal and disordered human erythropoiesis. Curr Opin Hematol 24:159-166. \u003ca href=\"https:\/\/doi.org\/10.1097\/MOH.0000000000000328\" target=\"_blank\" rel=\"noopener\"\u003e10.1097\/MOH.0000000000000328\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419268461,"sku":"HHM-EP-0010","price":166.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641427165549,"sku":"HHM-EP-0100","price":948.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EPO-500x500.png?v=1791572768"},{"product_id":"recombinant-human-scf-protein-bhp22200012","title":"Recombinant Human SCF Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human SCF Protein\u003c\/strong\u003e is a tag-free, full-length human SCF expressed in engineered \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated monomer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eSCF is used in hematopoiesis research, including culture and expansion of hematopoietic stem cells, and in studies of immune-cell development, mast cell biology and tissue repair. Its activity is confirmed by dose-dependent stimulation of TF-1 cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eSCF is a monomeric glycoprotein of 35 to 45 kDa produced by many cell types, including melanocytes, hepatocytes and bone cells. It promotes mast cell development and the survival and proliferation of melanocytes, and has a central role in hematopoiesis, particularly in the development of hematopoietic stem cells.\u003c\/p\u003e\n\u003cp\u003eSCF has been linked to disease processes involving tissue remodeling and fibrosis, and it acts synergistically with other cytokines, including EPO and GM-CSF.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human SCF is a growth factor for hematopoiesis and immune-cell research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCulture and expansion of hematopoietic stem cells.\u003c\/li\u003e\n\u003cli\u003eHematopoiesis research, particularly development of hematopoietic stem cells.\u003c\/li\u003e\n\u003cli\u003eStudies of immune-cell development and function, including mast cell development.\u003c\/li\u003e\n\u003cli\u003eTissue repair and regeneration research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eSCF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eEngineered human cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e35–45 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eDose-dependent stimulation of human TF-1 cell proliferation (human erythroleukemic indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Stem Cell Culture, Cell Proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is determined by dose-dependent stimulation of the proliferation of human TF-1 cells, an erythroleukemic indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eKitamura T, Tange T, Terasawa T, et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol 140:323-334. \u003ca href=\"https:\/\/doi.org\/10.1002\/jcp.1041400219\" target=\"_blank\" rel=\"noopener\"\u003e10.1002\/jcp.1041400219\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eAli S, Ali S (2007) Role of c-kit\/SCF in cause and treatment of gastrointestinal stromal tumors (GIST). Gene 401:38-45. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.gene.2007.06.017\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.gene.2007.06.017\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419596141,"sku":"HHM-SF-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641427329389,"sku":"HHM-SF-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SCF-500x500.png?v=1791572775"},{"product_id":"recombinant-human-tpo-protein-bhp22200013","title":"Recombinant Human TPO Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Cytokine\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human TPO Protein\u003c\/strong\u003e (thrombopoietin) is expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated monomer. It is tag-free, animal-free and xeno-free, and is lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eTPO is used for HSPC survival and expansion in SCF\/FLT3L-containing systems, for megakaryocytic commitment and maturation, for proplatelet and platelet-biogenesis studies and for MPL signaling research. Expression in human cells supports glycosylation of the full-length protein, activity is confirmed in Mo7e cells, and four peer-reviewed studies report use of this TPO.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThrombopoietin is a heavily glycosylated cytokine made mainly by the liver. It is the principal ligand of the MPL receptor on hematopoietic stem\/progenitor cells and megakaryocytic progenitors, where it activates JAK2\/STAT5, MAPK and PI3K signaling.\u003c\/p\u003e\n\u003cp\u003eIn culture, TPO helps maintain and expand HSPCs and promotes the growth, commitment and maturation of megakaryocytes toward platelet formation. What it does at a given point depends on the developmental stage, the cytokines used with it and the culture format.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eTPO is used from early HSPC culture through megakaryocyte maturation, usually with SCF and FLT3 Ligand at early stages. The table shows typical placements; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of TPO\u003c\/th\u003e\n\u003cth\u003eCompanion context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCord-blood CD34+ culture\u003c\/td\u003e\n\u003ctd\u003eStem\/progenitor maintenance\u003c\/td\u003e\n\u003ctd\u003eSCF and FLT3L, plus protocol-specific factors\u003c\/td\u003e\n\u003ctd\u003eExpansion, CD34\/CD38\/CD90, CFU, engraftment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ehPSC hematopoietic differentiation\u003c\/td\u003e\n\u003ctd\u003ePost-hemogenic progenitor support\u003c\/td\u003e\n\u003ctd\u003eSCF, FLT3L and lineage-stage cytokines\u003c\/td\u003e\n\u003ctd\u003eCD43, CD45, CD34, multilineage potential\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMegakaryocyte differentiation\u003c\/td\u003e\n\u003ctd\u003eCommitment and maturation\u003c\/td\u003e\n\u003ctd\u003eSCF early; later cytokine withdrawal by protocol\u003c\/td\u003e\n\u003ctd\u003eCD41, CD42b, polyploidy, proplatelets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMo7e bioassay\u003c\/td\u003e\n\u003ctd\u003eDose-dependent proliferation\u003c\/td\u003e\n\u003ctd\u003eDefined assay medium\u003c\/td\u003e\n\u003ctd\u003eProliferation and relative potency\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eConfirm the responsive cell stage and MPL receptor context.\u003c\/li\u003e\n\u003cli\u003eTake conditions from published or in-house validated work and titrate TPO for the cell source and endpoint.\u003c\/li\u003e\n\u003cli\u003eControl timing, medium, matrix and companion factors.\u003c\/li\u003e\n\u003cli\u003eInclude vehicle\/no-factor and pathway-response controls, and measure phenotype and function rather than cell number alone.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eTPO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eThrombopoietin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eHematopoietic cytokine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ Xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e80–85 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human Mo7e cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEC50\u003c\/th\u003e\n\u003ctd\u003e0.36–0.53 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Cell Proliferation, Colony-Forming Unit Assay, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eActivity is determined as proliferation of human Mo7e cells in response to TPO, giving an EC50 of 0.36–0.53 ng\/mL in the manufacturer's assay.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003ede Sauvage FJ, Hass PE, Spencer SD, et al. (1994) Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand. Nature 369:533-538. \u003ca href=\"https:\/\/doi.org\/10.1038\/369533a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/369533a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eLok S, Kaushansky K, Holly RD, et al. (1994) Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo. Nature 369:565-568. \u003ca href=\"https:\/\/doi.org\/10.1038\/369565a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/369565a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419628909,"sku":"HHM-TP-0010","price":124.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641428574573,"sku":"HHM-TP-0100","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TPO-500x500.png?v=1791572780"},{"product_id":"recombinant-human-hgf-protein-bhp22200014","title":"Recombinant Human HGF Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human HGF Protein\u003c\/strong\u003e is a tag-free, full-length human HGF expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated monomer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eHGF is used in research on liver regeneration, wound healing through cell proliferation and migration, tumor growth and metastasis, and differentiation of stem cells into hepatocytes and other cell types. Its activity is confirmed by stimulation of 4MBr-5 monkey epithelial cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eHGF is a pleiotropic protein of the plasminogen subfamily of S1 peptidases. Produced by mesenchymal cells, it strongly stimulates the proliferation of mature hepatocytes and also acts as a growth factor for many other tissues and cell types. HGF signals through MET, a transmembrane tyrosine kinase receptor.\u003c\/p\u003e\n\u003cp\u003eHuman HGF is secreted as an inactive single-chain propeptide of 728 amino acids (aa). It regulates proliferation, cell scattering, morphogenesis and survival, and is a key mitogen in liver regeneration, especially after partial hepatectomy and other liver injury.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human HGF is a growth factor for regeneration, proliferation and differentiation research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eLiver regeneration research, based on the strong mitogenic effect of HGF on hepatocytes.\u003c\/li\u003e\n\u003cli\u003eWound-healing models driven by cell proliferation and migration.\u003c\/li\u003e\n\u003cli\u003eCancer research on tumor growth and metastasis involving HGF.\u003c\/li\u003e\n\u003cli\u003eDifferentiation of stem cells into hepatocytes and other cell types.\u003c\/li\u003e\n\u003cli\u003eDrug-discovery studies in liver disease and cancer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eHGF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003ePlasminogen subfamily of S1 peptidases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e70 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of proliferation of the monkey epithelial cell line 4MBr-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Proliferation, Cell Migration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of 4MBr-5, a monkey epithelial cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eDi Renzo MF, Narsimhan RP, Olivero M, et al. (1991) Expression of the Met\/HGF receptor in normal and neoplastic human tissues. Oncogene 6:1997-2003.\u003c\/li\u003e\n\u003cli\u003eHammond DE, Carter S, Clague MJ (2004) Met receptor dynamics and signalling. Curr Top Microbiol Immunol 286:21-44. \u003ca href=\"https:\/\/doi.org\/10.1007\/978-3-540-69494-6_2\" target=\"_blank\" rel=\"noopener\"\u003e10.1007\/978-3-540-69494-6_2\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eBottaro DP, Rubin JS, Faletto DL, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science 251:802-804. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.1846706\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.1846706\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419891053,"sku":"HST-HF-0010","price":232.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641428640109,"sku":"HST-HF-0100","price":1036.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HGF-500x500.png?v=1791572786"},{"product_id":"recombinant-human-noggin-protein-bhp22200015","title":"Recombinant Human Noggin Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · BMP Antagonist\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human Noggin Protein\u003c\/strong\u003e is a full-length, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. Noggin is a secreted antagonist of bone morphogenetic proteins (BMPs). The protein is made in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eResearchers use Noggin to block extracellular BMP signaling during neural induction and neural progenitor work, to keep a low-BMP environment in intestinal, gastric, neural and other organoid systems, and to study BMP-dependent patterning of neural, mesodermal and skeletal tissues. It also serves as a defined antagonist for titrating BMP-2\/BMP-4 responses in signaling assays. Function is confirmed in a cell-based BMP-4 antagonism assay.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eNoggin is a secreted cystine-knot protein that binds selected BMPs, most notably BMP-2 and BMP-4, and prevents them from reaching their type I and type II receptors. Less BMP–receptor engagement means less SMAD1\/5\/8 activation, which shifts cell-fate decisions in a way that depends strongly on developmental stage and context.\u003c\/p\u003e\n\u003cp\u003eIn vivo, Noggin has key roles in neural patterning, skeletal development and the formation of cartilage and joints. In culture it is not a universal differentiation factor: the outcome depends on the endogenous BMP level, Noggin concentration, matrix, cell state and companion pathways such as FGF, EGF, WNT and Activin\/Nodal.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eNoggin is used wherever BMP signaling needs to be reduced at a defined stage, from neural induction to organoid expansion. The table shows where it is typically placed in a workflow; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of Noggin\u003c\/th\u003e\n\u003cth\u003eCompanion signals and context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeural induction\u003c\/td\u003e\n\u003ctd\u003eLowers BMP-dependent signals toward non-neural fates\u003c\/td\u003e\n\u003ctd\u003eOften combined with TGF-β\/Activin\/Nodal inhibition in dual-SMAD protocols\u003c\/td\u003e\n\u003ctd\u003ePAX6, SOX1, NESTIN, neural rosettes and lineage competence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoid expansion\u003c\/td\u003e\n\u003ctd\u003eKeeps a tissue-specific low-BMP niche\u003c\/td\u003e\n\u003ctd\u003eEGF, FGF, WNT\/R-spondin or tissue-specific factors\u003c\/td\u003e\n\u003ctd\u003eOrganoid-forming efficiency, morphology, lineage markers and function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBMP signaling assay\u003c\/td\u003e\n\u003ctd\u003eExtracellular antagonist\u003c\/td\u003e\n\u003ctd\u003eBMP-2 or BMP-4 dose-response control\u003c\/td\u003e\n\u003ctd\u003epSMAD1\/5\/8, ID genes, alkaline phosphatase and reporter activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkeletal\/chondrogenic research\u003c\/td\u003e\n\u003ctd\u003eModulates BMP-driven differentiation\u003c\/td\u003e\n\u003ctd\u003eMatrix and stage-specific chondrogenic signals\u003c\/td\u003e\n\u003ctd\u003eALP, SOX9, COL2A1, mineralization and morphology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the developmental stage and confirm that the target cells depend on BMP signaling at that stage.\u003c\/li\u003e\n\u003cli\u003eStart from a validated or internally qualified concentration, then run a dose response for the cell source, basal medium and readout.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors, since signal order and duration change the outcome; include no-factor, vehicle and BMP-response controls.\u003c\/li\u003e\n\u003cli\u003eAssess identity, viability, signaling and function rather than cell number alone. No universal working concentration or EC50 applies without lot- or protocol-specific evidence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eNoggin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eSecreted cystine-knot BMP antagonist\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e65 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInhibition of BMP-4-induced alkaline phosphatase in ATDC-5 cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, 3D cell culture, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of Noggin to inhibit BMP-4-induced alkaline phosphatase production in ATDC-5 mouse chondrogenic cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eGroppe J, et al. (2002) Structural basis of BMP signalling inhibition by the cystine knot protein Noggin. Nature 420:636-42. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature01245\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature01245\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eBrunet LJ, et al. (1998) Noggin, cartilage morphogenesis, and joint formation in the mammalian skeleton. Science 280:1455-7. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.280.5368.1455\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.280.5368.1455\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChambers SM, et al. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27:275-80. \u003ca href=\"https:\/\/doi.org\/10.1038\/nbt.1529\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nbt.1529\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSato T, Vries RG, Snippert HJ, et al. (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262-265. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature07935\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature07935\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMcCracken KW, Catá EM, Crawford CM, et al. (2014) Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature 516:400-404. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature13863\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature13863\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419956589,"sku":"HST-NG-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641429066093,"sku":"HST-NG-0100","price":758.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/noggin-500x500.png?v=1791572791"},{"product_id":"recombinant-human-osm-protein-bhp22200016","title":"Recombinant Human OSM Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · IL-6 Family Cytokine\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human OSM Protein\u003c\/strong\u003e (oncostatin M) is a tag-free, full-length human OSM expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e as a glycosylated protein. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eOSM is used to study inflammation and immune responses, hematopoiesis including myeloid differentiation, neuroprotection and neuronal survival, and cancer biology, where it can promote or suppress tumor growth depending on context. Its activity is confirmed by stimulation of TF-1 cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eOncostatin M (OSM) is a 30 kDa secreted cytokine of the interleukin 6 (IL-6) family. It inhibits the proliferation of several tumor cell lines, yet stimulates proliferation of AIDS-KS cells.\u003c\/p\u003e\n\u003cp\u003eOSM regulates cytokine production by endothelial cells, including IL-6, G-CSF and GM-CSF, and has important roles in inflammation, neuroprotection, hematopoiesis, metabolism and development.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human OSM is a versatile cytokine for immunology, hematopoiesis and cancer-biology research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInflammation research, including regulation of other inflammatory cytokines.\u003c\/li\u003e\n\u003cli\u003eHematopoiesis studies, particularly differentiation of myeloid cells.\u003c\/li\u003e\n\u003cli\u003eNeuroprotection and neuronal-survival research.\u003c\/li\u003e\n\u003cli\u003eCancer biology, where OSM can promote or suppress tumor growth depending on context.\u003c\/li\u003e\n\u003cli\u003eDrug-discovery studies in inflammatory disease and cancer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eOSM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eOncostatin M\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eIL-6 family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e30 kDa homotrimer. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation (human erythroleukemic indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of human TF-1 cells, an erythroleukemic indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eMalik N, Kallestad JC, Gunderson NL, et al. (1989) Molecular cloning, sequence analysis, and functional expression of a novel growth regulator, oncostatin M. Mol Cell Biol 9:2847-2853. \u003ca href=\"https:\/\/doi.org\/10.1128\/mcb.9.7.2847-2853.1989\" target=\"_blank\" rel=\"noopener\"\u003e10.1128\/mcb.9.7.2847-2853.1989\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRose TM, Bruce AG (1991) Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc Natl Acad Sci U S A 88:8641-8645. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.88.19.8641\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.88.19.8641\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641420022125,"sku":"HST-O-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641429950829,"sku":"HST-O-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/OSM-500x500.png?v=1791572797"},{"product_id":"recombinant-human-tgf-beta1-protein-bhp22200017","title":"Recombinant Human TGF beta1 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human TGF beta1 Protein\u003c\/strong\u003e (TGF-β1) is the mature, non-glycosylated homodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is manufactured in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eTGF-β1 is used to study regulatory T-cell differentiation, tolerance and immune suppression, fibroblast activation and matrix deposition, epithelial–mesenchymal transition (EMT) and TGFBR–SMAD2\/3 signaling. Activity is confirmed in a cell-based HT-2 assay, and two peer-reviewed studies report the use of this TGF-β1.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eTGF-β1 is the most abundant TGF-β isoform in mammals and is secreted as part of a latent complex that must be activated before it can signal. The active homodimer binds TGFBR2, which recruits and activates TGFBR1\/ALK5. This leads to phosphorylation of SMAD2 and SMAD3, which partner with SMAD4 to regulate transcription.\u003c\/p\u003e\n\u003cp\u003eTGF-β1 also engages non-SMAD pathways, including MAPK and PI3K, and its effects depend on cell identity and microenvironment. It is studied in immune tolerance, regulatory T-cell biology, fibrosis, EMT, extracellular-matrix regulation, myogenesis and stem\/progenitor differentiation.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eTGF-β1 is used where SMAD2\/3 signaling drives immune regulation, matrix programs or epithelial plasticity. The table summarizes typical workflow placement; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of TGF-β1\u003c\/th\u003e\n\u003cth\u003eCompanion signals and context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRegulatory T-cell differentiation\u003c\/td\u003e\n\u003ctd\u003eTGF-β-dependent FOXP3 induction\u003c\/td\u003e\n\u003ctd\u003eIL-2 and TCR stimulation\u003c\/td\u003e\n\u003ctd\u003eFOXP3, CD25, suppressive function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFibroblast activation\u003c\/td\u003e\n\u003ctd\u003eDrives the myofibroblast and matrix program\u003c\/td\u003e\n\u003ctd\u003eDefined serum context\u003c\/td\u003e\n\u003ctd\u003eα-SMA, COL1A1, fibronectin and contraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEMT model\u003c\/td\u003e\n\u003ctd\u003eInduces epithelial plasticity\u003c\/td\u003e\n\u003ctd\u003eTime course and pathway inhibitors\u003c\/td\u003e\n\u003ctd\u003eE-cadherin, vimentin, migration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSMAD signaling assay\u003c\/td\u003e\n\u003ctd\u003eAcute receptor activation\u003c\/td\u003e\n\u003ctd\u003eALK5 inhibitor control\u003c\/td\u003e\n\u003ctd\u003epSMAD2\/3, nuclear translocation, target genes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the responsive cell stage and confirm that the cells express the relevant receptors.\u003c\/li\u003e\n\u003cli\u003eBegin with a validated protocol, then run a dose response for the actual cell source, medium and endpoint.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors, since signal order and duration can change lineage or functional outcomes; include no-factor, vehicle and pathway-response controls.\u003c\/li\u003e\n\u003cli\u003eMeasure phenotype and function rather than cell number alone. No EC50 or universal working concentration applies without lot- or protocol-specific evidence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eTGF beta1 (also known as TGF-β1)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eTGF-β1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β superfamily\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e25 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in 10 mM sodium acetate without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInhibition of IL-4-induced proliferation in mouse HT-2 cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Signaling Studies, Epithelial-Mesenchymal Transition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is evaluated by the ability of TGF-β1 to inhibit IL-4-induced proliferation of mouse HT-2 cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eMassagué J (2012) TGFβ signalling in context. Nat Rev Mol Cell Biol 13:616-630. \u003ca href=\"https:\/\/doi.org\/10.1038\/nrm3434\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nrm3434\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eOshimori N, Fuchs E (2012) The harmonies played by TGF-β in stem cell biology. Cell Stem Cell 11:751-764. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.stem.2012.11.001\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.stem.2012.11.001\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641420513645,"sku":"HST-TB1-0010","price":134.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641430606189,"sku":"HST-TB1-0100","price":938.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/tgfb1-500x500.webp?v=1791572801"},{"product_id":"recombinant-human-gm-csf-protein-bhp22200018","title":"Recombinant Human GM-CSF Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Cytokine\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human GM-CSF Protein\u003c\/strong\u003e is a full-length, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is manufactured in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eGM-CSF is widely used with IL-4 to generate immature monocyte-derived dendritic cells, and in studies of myeloid progenitor survival, proliferation and differentiation, antigen presentation, cytokine production and GM-CSF receptor signaling. Expression in human cells supports mammalian folding and glycosylation. Activity is confirmed by TF-1 cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eGM-CSF (CSF2) is a four-helix-bundle cytokine produced by activated T cells, macrophages, endothelial cells and stromal cells. Binding to its receptor complex triggers JAK2\/STAT5, MAPK and PI3K signaling, which supports survival, proliferation and functional maturation of myeloid progenitors and mature myeloid cells.\u003c\/p\u003e\n\u003cp\u003eIn vitro, GM-CSF is a standard factor for monocyte-derived dendritic-cell generation, often together with IL-4, and for studying granulocyte and macrophage lineage responses. Results depend on cell source, cytokine dose, exposure time and companion signals.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eGM-CSF supports responsive myeloid populations, from progenitors to monocyte-derived dendritic cells. The table summarizes where it is typically placed in a workflow; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of GM-CSF\u003c\/th\u003e\n\u003cth\u003eCompanion signals and context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMonocyte-derived dendritic cells\u003c\/td\u003e\n\u003ctd\u003eSupports early differentiation\u003c\/td\u003e\n\u003ctd\u003eIL-4; later a maturation cocktail\u003c\/td\u003e\n\u003ctd\u003eCD11c, HLA-DR, CD80\/CD86, morphology and antigen presentation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMyeloid progenitor culture\u003c\/td\u003e\n\u003ctd\u003eSurvival and lineage support\u003c\/td\u003e\n\u003ctd\u003eSCF, IL-3 or lineage-specific factors\u003c\/td\u003e\n\u003ctd\u003eCell count, viability, CFU, CD14\/CD15\/CD33\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTF-1 bioassay\u003c\/td\u003e\n\u003ctd\u003eReceptor-dependent proliferation\u003c\/td\u003e\n\u003ctd\u003eDefined low-serum assay conditions\u003c\/td\u003e\n\u003ctd\u003eDose response, viability and proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMyeloid signaling\u003c\/td\u003e\n\u003ctd\u003eAcute receptor activation\u003c\/td\u003e\n\u003ctd\u003eTime-course and pathway controls\u003c\/td\u003e\n\u003ctd\u003epSTAT5, pERK, pAKT and target genes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the responsive cell stage and confirm that the cells express the GM-CSF receptor.\u003c\/li\u003e\n\u003cli\u003eBegin with a validated protocol, then run a dose response for the actual cell source, medium and endpoint.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors such as IL-4, since signal order and duration can change lineage or functional outcomes; include no-factor, vehicle and pathway-response controls.\u003c\/li\u003e\n\u003cli\u003eMeasure phenotype and function rather than cell number alone. No EC50 or universal working concentration applies without lot- or protocol-specific evidence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eGM-CSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eCSF2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eFour-helix-bundle cytokine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e15–36 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Antigen Presentation Assay, Cell Proliferation, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by dose-dependent stimulation of human TF-1 cell proliferation.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eWang X, et al. (2018) Dendritic Cells Are Critical for the Activation and Expansion of Vδ2+ T Cells After Allogeneic Hematopoietic Transplantation. Front Immunol 9:2528. \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2018.02528\" target=\"_blank\" rel=\"noopener\"\u003e10.3389\/fimmu.2018.02528\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWong GG, Witek JS, Temple PA, et al. (1985) Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228:810-815. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.3923623\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.3923623\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003evan de Laar L, Coffer PJ, Woltman AM (2012) Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy. Blood 119:3383-3393. \u003ca href=\"https:\/\/doi.org\/10.1182\/blood-2011-11-370130\" target=\"_blank\" rel=\"noopener\"\u003e10.1182\/blood-2011-11-370130\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eZhan Y, Lew AM, Chopin M (2019) The Pleiotropic Effects of the GM-CSF Rheostat on Myeloid Cell Differentiation and Function: More Than a Numbers Game. Front Immunol 10:2679. \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2019.02679\" target=\"_blank\" rel=\"noopener\"\u003e10.3389\/fimmu.2019.02679\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641421496685,"sku":"HHM-GM-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641430737261,"sku":"HHM-GM-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GMCSF-500x500.png?v=1791572807"},{"product_id":"recombinant-human-g-csf-protein-bhp22200019","title":"Recombinant Human G-CSF Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Colony-Stimulating Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human G-CSF Protein\u003c\/strong\u003e (granulocyte colony-stimulating factor) is a tag-free, full-length human G-CSF expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated monomer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eG-CSF is a research tool for hematopoiesis and stem-cell biology, including hematopoietic stem cell mobilization, production of granulocytes and monocytes-macrophages, and immune-cell development. Its activity is confirmed by stimulation of murine M-NFS-60 cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eGranulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor of the CSF family. It is expressed mainly by the monocyte\/macrophage lineage and also by cells of mesodermal origin, such as vascular endothelial cells, fibroblasts and mesothelial cells.\u003c\/p\u003e\n\u003cp\u003eIn hematopoiesis, G-CSF controls the production, differentiation and function of two related white-cell populations, the granulocytes and the monocytes-macrophages. It also mobilizes hematopoietic stem cells from the bone marrow into the peripheral blood.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human G-CSF is used in hematopoiesis and stem-cell research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHematopoietic stem cell mobilization studies.\u003c\/li\u003e\n\u003cli\u003eHematopoiesis research on production and differentiation of granulocytes and monocytes-macrophages.\u003c\/li\u003e\n\u003cli\u003eStem-cell biology research.\u003c\/li\u003e\n\u003cli\u003eStudies of immune-cell development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eG-CSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eGranulocyte colony-stimulating factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eCSF family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e21–25 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of murine M-NFS-60 cell proliferation (mouse myeloid leukemia indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of murine M-NFS-60 cells, a mouse myeloid leukemia indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eNagata S, Tsuchiya M, Asano S, et al. (1986) Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature 319:415-418. \u003ca href=\"https:\/\/doi.org\/10.1038\/319415a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/319415a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eShirafuji N, Asano S, Matsuda S, et al. (1989) A new bioassay for human granulocyte colony-stimulating factor (hG-CSF) using murine myeloblastic NFS-60 cells as targets and estimation of its levels in sera from normal healthy persons and patients with infectious and hematological disorders. Exp Hematol 17:116-119.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641421594989,"sku":"HHM-GC-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641431327085,"sku":"HHM-GC-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GCSF-500x500.png?v=1791572814"},{"product_id":"recombinant-human-m-csf-protein-bhp22200020","title":"Recombinant Human M-CSF Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Colony-Stimulating Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human M-CSF Protein\u003c\/strong\u003e (macrophage colony-stimulating factor) is a tag-free, full-length human M-CSF expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated homodimer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eM-CSF is used to study hematopoiesis, particularly proliferation, differentiation and survival of monocytes, macrophages and bone-marrow progenitors, as well as innate immune responses and tissue repair processes such as wound healing and bone remodeling. Its activity is confirmed by stimulation of murine M-NFS-60 cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eMacrophage colony-stimulating factor (M-CSF) is a hematopoietic factor produced by many cell types, including lymphocytes, fibroblasts, endothelial cells, myoblasts, monocytes and osteoblasts.\u003c\/p\u003e\n\u003cp\u003eIt controls the proliferation, differentiation and survival of monocytes, macrophages and bone-marrow progenitor cells, and it takes part in regulating innate immunity and in tissue repair and bone remodeling.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human M-CSF is a growth factor for monocyte, macrophage and immunology research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHematopoiesis research on proliferation and differentiation of monocytes and macrophages.\u003c\/li\u003e\n\u003cli\u003eStudies of innate immune responses.\u003c\/li\u003e\n\u003cli\u003eTissue repair and regeneration research, including wound healing and bone remodeling.\u003c\/li\u003e\n\u003cli\u003eDrug-discovery studies in inflammatory disease and tissue regeneration.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eM-CSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eMacrophage colony-stimulating factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e30–40 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of murine M-NFS-60 cell proliferation (mouse myeloid leukemia indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Differentiation Assay, Cell Proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of murine M-NFS-60 cells, a mouse myeloid leukemia indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eKawasaki ES, Ladner MB, Wang AM, et al. (1985) Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1). Science 230:291-296. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.2996129\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.2996129\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eStein J, Borzillo GV, Rettenmier CW (1990) Direct stimulation of cells expressing receptors for macrophage colony-stimulating factor (CSF-1) by a plasma membrane-bound precursor of human CSF-1. Blood 76:1308-1314.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641421824365,"sku":"HHM-MC-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641431425389,"sku":"HHM-MC-0100","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MSCF-500x500.png?v=1791572817"},{"product_id":"recombinant-human-tnf-alpha-protein-bhp22200021","title":"Recombinant Human TNF alpha Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Cytokine\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human TNF alpha Protein\u003c\/strong\u003e (TNF-α) is a tag-free, full-length human TNF-α expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated homotrimer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eTNF-α is a potent pro-inflammatory cytokine used to study inflammation, activation of immune cells and cell death, particularly of tumor cells. Its activity is confirmed by cytotoxicity toward L-929 cells in the presence of actinomycin D.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eTumor necrosis factor alpha (TNF-α) signals through the receptors TNFRSF1A (TNFR1) and TNFRSF1B. It is secreted mainly by macrophages and is also produced by monocytes, keratinocytes and various tumor cells.\u003c\/p\u003e\n\u003cp\u003eTNF-α induces fever, either directly or through release of interleukin-1, and has been linked to cachexia. Under certain conditions it can stimulate cell proliferation and induce differentiation, and it can impair regulatory T cell (Treg) function in rheumatoid arthritis through FOXP3 dephosphorylation.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human TNF-α is a pro-inflammatory cytokine for immunology and cell-death research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInflammation research on pro-inflammatory signaling.\u003c\/li\u003e\n\u003cli\u003eImmune-response studies, including activation of immune cells.\u003c\/li\u003e\n\u003cli\u003eCell-death research, particularly in tumor cells.\u003c\/li\u003e\n\u003cli\u003eDrug-discovery studies in inflammatory disease and cancer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eTNF alpha\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eTNF-α; TNFα\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated homotrimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e17 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eCytotoxicity in L-929 cells in the presence of actinomycin D\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cytotoxicity Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by cytotoxicity in L-929 cells in the presence of actinomycin D.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003ePennica D, Nedwin GE, Hayflick JS, et al. (1984) Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature 312:724-729. \u003ca href=\"https:\/\/doi.org\/10.1038\/312724a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/312724a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNie H, Zheng Y, Li R, et al. (2013) Phosphorylation of FOXP3 controls regulatory T cell function and is inhibited by TNF-α in rheumatoid arthritis. Nat Med 19:322-328. \u003ca href=\"https:\/\/doi.org\/10.1038\/nm.3085\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nm.3085\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFriedmann E, Hauben E, Maylandt K, et al. (2006) SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production. Nat Cell Biol 8:843-848. \u003ca href=\"https:\/\/doi.org\/10.1038\/ncb1440\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/ncb1440\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641421889901,"sku":"HIM-TNa-0010","price":90.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641431916909,"sku":"HIM-TNa-0100","price":644.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TNFa-500x500.png?v=1791572822"},{"product_id":"recombinant-human-ifn-gamma-protein-bhp22200022","title":"Recombinant Human IFN gamma Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Type II Interferon\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IFN gamma Protein\u003c\/strong\u003e (IFN-γ) is a tag-free, full-length human IFN-γ expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated dimer. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIFN-γ is used in research on innate and adaptive immunity, immunomodulation and immune-cell activation, cell signaling and immunotherapy. It is particularly suited to activating human CIK cells together with IL-2. Its activity is shown by STAT1 phosphorylation in human lung microvascular endothelial cells.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterferon gamma (IFN-γ) is a soluble, dimeric cytokine of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells, NKT cells and NK cells.\u003c\/p\u003e\n\u003cp\u003eBy acting on antigen-presenting cells and on B and T lymphocytes that recognize antigen, IFN-γ shapes the antigen-specific stages of the immune response. It is critical for innate and adaptive immunity against viral, protozoal and bacterial infections.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human IFN-γ is an immunomodulatory cytokine for immunology and cell-signaling research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunomodulation studies, including activation of immune cells.\u003c\/li\u003e\n\u003cli\u003eActivation of human CIK cells in combination with IL-2.\u003c\/li\u003e\n\u003cli\u003eResearch on immune responses and cell signaling pathways.\u003c\/li\u003e\n\u003cli\u003eImmunotherapy research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIFN gamma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eIFN-γ; IFNγ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated dimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eType II interferon\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e21–25 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of STAT1 phosphorylation (Tyr701) in human lung microvascular endothelial cells (HLMVECs), detected by Western blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Signaling Studies, T Cell Activation Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is shown by stimulation of STAT1 phosphorylation in human lung microvascular endothelial cells (HLMVECs). Cells were serum-starved overnight in EBM-2 medium containing 2% FBS, treated with IFN-γ at 0.5 to 500 ng\/mL for 5 minutes, and phosphorylated STAT1 (Tyr701) was detected by Western blot with a phospho-STAT1 antibody.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eGray PW, Goeddel DV (1982) Structure of the human immune interferon gene. Nature 298:859-863. \u003ca href=\"https:\/\/doi.org\/10.1038\/298859a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/298859a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eJiang J, Wu C, Lu B (2013) Cytokine-induced killer cells promote antitumor immunity. J Transl Med 11:83. \u003ca href=\"https:\/\/doi.org\/10.1186\/1479-5876-11-83\" target=\"_blank\" rel=\"noopener\"\u003e10.1186\/1479-5876-11-83\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"100 ug","offer_id":67641422086509,"sku":"HST-IFg-0100","price":170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/INFg-500x500.png?v=1791572825"},{"product_id":"recombinant-human-il-1-beta-protein-bhp22200023","title":"Recombinant Human IL-1 beta Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Interleukin\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-1 beta Protein\u003c\/strong\u003e (IL-1β) is a tag-free, full-length human IL-1β expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e as a glycosylated protein. It is produced in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-1β is a potent pro-inflammatory cytokine used to study inflammation, activation of immune cells such as T cells and macrophages, tissue repair, cell signaling pathways and the pathogenesis of inflammatory disease. Its activity is confirmed by stimulation of mouse D10S cell proliferation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-1 comprises two pleiotropic cytokines, IL-1α and IL-1β, which bind the same receptor and have similar, if not identical, biological properties. IL-1β is a pro-inflammatory cytokine produced by many cell types, including monocytes, tissue macrophages, keratinocytes and other epithelial cells.\u003c\/p\u003e\n\u003cp\u003eTheir activities include stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human IL-1β is a pro-inflammatory cytokine for immunology and inflammation research.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInflammation research, including the pathogenesis of inflammatory disease.\u003c\/li\u003e\n\u003cli\u003eImmune-response studies, including activation of T cells and macrophages.\u003c\/li\u003e\n\u003cli\u003eTissue repair and regeneration research.\u003c\/li\u003e\n\u003cli\u003eStudies of cell signaling pathways.\u003c\/li\u003e\n\u003cli\u003eDrug-discovery studies in inflammatory disease.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-1 beta\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eIL-1β\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e18–25 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of mouse D10S cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of mouse D10S cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBensi G, Raugei G, Palla E, et al. (1987) Human interleukin-1 beta gene. Gene 52:95-101. \u003ca href=\"https:\/\/doi.org\/10.1016\/0378-1119(87)90398-2\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/0378-1119(87)90398-2\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eVan Damme J, De Ley M, Opdenakker G, et al. (1985) Homogeneous interferon-inducing 22K factor is related to endogenous pyrogen and interleukin-1. Nature 314:266-268. \u003ca href=\"https:\/\/doi.org\/10.1038\/314266a0\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/314266a0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKornman KS (2006) Interleukin 1 genetics, inflammatory mechanisms, and nutrigenetic opportunities to modulate diseases of aging. Am J Clin Nutr 83:475S-483S. \u003ca href=\"https:\/\/doi.org\/10.1093\/ajcn\/83.2.475S\" target=\"_blank\" rel=\"noopener\"\u003e10.1093\/ajcn\/83.2.475S\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641422152045,"sku":"HIM-IB1-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641432473965,"sku":"HIM-IB1-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL1b-500x500.png?v=1791572829"},{"product_id":"recombinant-human-il-2-protein-bhp22200024","title":"Recombinant Human IL-2 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Interleukin\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-2 Protein\u003c\/strong\u003e is a full-length, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is manufactured in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-2 is used to support proliferation and survival of activated T cells, to expand NK cells for functional and cytotoxicity assays, to expand Vδ2+ and other γδ T-cell populations, and to study IL-2 receptor, STAT5 and survival signaling. Activity is confirmed by CTLL-2 cell proliferation, and two peer-reviewed studies identify this IL-2 or its catalog number.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterleukin-2 is a glycosylated, short-range cytokine produced mainly by activated T cells. It drives proliferation and survival of activated T cells, can stimulate NK cells and also contributes to the maintenance of regulatory T cells.\u003c\/p\u003e\n\u003cp\u003eIL-2 signals through IL-2 receptor complexes and activates JAK1\/JAK3–STAT5 together with PI3K–AKT and MAPK pathways. Receptor composition and IL-2 concentration strongly shape which cells respond, and outcomes also depend on activation state, culture density, companion cytokines such as IL-7, IL-15 and IL-21, and exposure time.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-2 supports the expansion and survival of several lymphocyte populations and is a standard ligand for IL-2 receptor signaling studies. The table summarizes typical workflow placement; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of IL-2\u003c\/th\u003e\n\u003cth\u003eCompanion signals and context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eActivated conventional T cells\u003c\/td\u003e\n\u003ctd\u003eProliferation and survival\u003c\/td\u003e\n\u003ctd\u003eTCR\/CD3-CD28 or antigen activation\u003c\/td\u003e\n\u003ctd\u003eExpansion, viability, phenotype, cytokines and cytotoxicity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNK-cell culture\u003c\/td\u003e\n\u003ctd\u003eGrowth and activation support\u003c\/td\u003e\n\u003ctd\u003eIL-15 and\/or IL-21 depending on protocol\u003c\/td\u003e\n\u003ctd\u003eCD56\/CD16 phenotype, degranulation, killing and cytokines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eγδ T-cell culture\u003c\/td\u003e\n\u003ctd\u003eExpansion after phosphoantigen-related activation\u003c\/td\u003e\n\u003ctd\u003eZoledronate\/pamidronate or IPP in protocol-specific systems\u003c\/td\u003e\n\u003ctd\u003eVδ2 frequency, proliferation, cytokines and tumor-cell killing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRegulatory T-cell research\u003c\/td\u003e\n\u003ctd\u003eContext-dependent survival and STAT5 support\u003c\/td\u003e\n\u003ctd\u003eDefined TCR stimulation and low-dose experimental design\u003c\/td\u003e\n\u003ctd\u003eFOXP3, CD25, suppressive function and pSTAT5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the immune-cell stage and confirm that the target population expresses the relevant IL-2 receptor components.\u003c\/li\u003e\n\u003cli\u003eStart from a peer-reviewed or internally qualified concentration, then run a dose response for the cell source, basal medium and readout.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion cytokines, since effects depend on signal order, duration and pathway interactions; include no-factor, vehicle and pathway-response controls.\u003c\/li\u003e\n\u003cli\u003eAssess identity, viability, signaling and function rather than cell number alone. No EC50 or universal working concentration applies without lot- or protocol-specific evidence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eInterleukin-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eInterleukin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 15 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eDose-dependent proliferation of mouse CTLL-2 cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, T Cell Activation Assay, Cell Proliferation, Cytotoxicity Assay, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eActivity is determined in a CTLL-2 assay, in which the protein drives proliferation of these mouse cytotoxic T cells in a dose-dependent way.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eMalek TR (2008) The biology of interleukin-2. Annu Rev Immunol 26:453-479. \u003ca href=\"https:\/\/doi.org\/10.1146\/annurev.immunol.26.021607.090357\" target=\"_blank\" rel=\"noopener\"\u003e10.1146\/annurev.immunol.26.021607.090357\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eBoyman O, Sprent J (2012) The role of interleukin-2 during homeostasis and activation of the immune system. Nat Rev Immunol 12:180-190. \u003ca href=\"https:\/\/doi.org\/10.1038\/nri3156\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nri3156\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWaldmann TA (2006) The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat Rev Immunol 6:595-601. \u003ca href=\"https:\/\/doi.org\/10.1038\/nri1901\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nri1901\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641422217581,"sku":"HCT-I2-0010","price":102.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641432572269,"sku":"HCT-I2-0100","price":407.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL2-500x500.png?v=1791572833"},{"product_id":"recombinant-human-il-3-protein-bhp22200025","title":"Recombinant Human IL-3 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Hematopoietic Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-3 Protein\u003c\/strong\u003e is a tag-free, non-glycosylated homodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-3 is used to study hematopoiesis and myeloid-lineage development, to culture and expand hematopoietic stem cells, and in research on immune-cell development and hematopoietic reconstitution after bone marrow transplantation. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterleukin-3 (IL-3) is a secreted protein from the group of hematopoietic growth factors and supports the natural immune response to disease. It drives multipotent hematopoietic stem cells toward myeloid rather than lymphoid progenitors (lymphoid differentiation is driven by IL-7). Together with cytokines such as EPO, GM-CSF, TPO and IL-6, it promotes proliferation of cells across the myeloid lineage, including erythrocytes, megakaryocytes, granulocytes, monocytes and dendritic cells.\u003c\/p\u003e\n\u003cp\u003eIL-3 also contributes to embryo implantation and placental development; lower IL-3 levels have been found in pregnant women with antiphospholipid syndrome (APS) and in animal models of APS. In addition, IL-3 takes part in the response of the organism to different types of stress.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-3 is used mainly in hematopoiesis, immunology and stem-cell research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHematopoiesis: stimulates proliferation and differentiation of hematopoietic stem cells into several blood-cell lineages.\u003c\/li\u003e\n\u003cli\u003eStem cell research: culture and expansion of hematopoietic stem cells.\u003c\/li\u003e\n\u003cli\u003eImmunology: regulation of the immune response, including development and function of immune cells.\u003c\/li\u003e\n\u003cli\u003eTransplantation research: studies of hematopoietic reconstitution after bone marrow transplantation.\u003c\/li\u003e\n\u003cli\u003eMyelopoiesis research: proliferation of myeloid-lineage cells together with other cytokines.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eInterleukin-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eHematopoietic growth factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e19 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation (human erythroleukemic indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Cell Proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of human TF-1 cells, a human erythroleukemic indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eYang YC, Ciarletta AB, Temple PA, et al. (1986) Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3. Cell 47:3-10. \u003ca href=\"https:\/\/doi.org\/10.1016\/0092-8674(86)90360-0\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/0092-8674(86)90360-0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMartinez-Moczygemba M, Huston DP (2003) Biology of common beta receptor-signaling cytokines: IL-3, IL-5, and GM-CSF. J Allergy Clin Immunol 112:653-665.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641422971245,"sku":"HCT-I3-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641433358701,"sku":"HCT-I3-0100","price":809.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL3-500x500.png?v=1791572840"},{"product_id":"recombinant-human-il-4-protein-bhp22200026","title":"Recombinant Human IL-4 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · IL-4 \/ IL-13 Family\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-4 Protein\u003c\/strong\u003e is a tag-free, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-4 is a strong immunomodulatory cytokine used to study the shaping of immune responses, allergic inflammation and asthma, and autoimmune disease, where it can promote or suppress responses depending on context. It is also used in drug-discovery research on inflammatory and autoimmune disease. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterleukin 4 (IL-4) is a 14–19 kDa glycosylated monomer of the IL-4 \/ IL-13 family. It is produced mainly by Th2-biased CD4+ T cells, mast cells, basophils and eosinophils.\u003c\/p\u003e\n\u003cp\u003eIL-4 has many roles: it contributes to several steps of B-cell activation and also acts on other cell types. It co-stimulates DNA synthesis, induces class II MHC expression on resting B cells, increases both secretion and surface expression of IgE and IgG1, and regulates expression of the low-affinity IgE Fc receptor (CD23) on lymphocytes and monocytes.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-4 is used mainly in immunology, inflammation and allergy research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunomodulation: studies of how IL-4 shapes the immune response.\u003c\/li\u003e\n\u003cli\u003eAllergic inflammation: research on the development of allergic inflammation and asthma.\u003c\/li\u003e\n\u003cli\u003eAutoimmune disease: IL-4 can promote or suppress autoimmune responses depending on context.\u003c\/li\u003e\n\u003cli\u003eDrug discovery: IL-4 as a target in inflammatory and autoimmune disease research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eInterleukin 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eIL-4 \/ IL-13 family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e14–19 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation (human erythroleukemic indicator cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of human TF-1 cells, a human erythroleukemic indicator cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\u003cli\u003eWang X, et al. (2018) Dendritic Cells Are Critical for the Activation and Expansion of Vδ2+ T Cells After Allogeneic Hematopoietic Transplantation. Front Immunol 9:2528. \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2018.02528\" target=\"_blank\" rel=\"noopener\"\u003e10.3389\/fimmu.2018.02528\u003c\/a\u003e\n\u003c\/li\u003e\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641423036781,"sku":"HCT-I4-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641433424237,"sku":"HCT-I4-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL4-500x500.png?v=1791572844"},{"product_id":"recombinant-human-il-6-protein-bhp22200027","title":"Recombinant Human IL-6 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Interleukin\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-6 Protein\u003c\/strong\u003e is a full-length, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is manufactured in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eTypical research uses include JAK\/STAT3 activation through IL-6R and gp130, the late stages of B-cell maturation into antibody-secreting cells, chosen hematopoietic and myeloid differentiation protocols, and models of acute-phase and inflammatory responses. Expression in human cells supports mammalian glycosylation, activity is confirmed by TF-1 cell proliferation, and one peer-reviewed study reports the use of this IL-6.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-6 is a pleiotropic, glycosylated cytokine produced by immune and stromal cell types. It binds membrane-bound or soluble IL-6R, forms a complex with gp130 and activates JAK\/STAT3, MAPK and PI3K pathways.\u003c\/p\u003e\n\u003cp\u003eIts roles span B-cell maturation and other immune-cell differentiation steps, the acute-phase response, hematopoietic progenitor biology and inflammatory programs that differ between tissues. Because it can act through both classical signaling and trans-signaling, experiments should distinguish the two; the outcome depends on IL-6R and soluble receptor availability, gp130, cell state, dose and exposure time.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-6 is used in signaling, immune-cell and hematopoietic research, and as a defined stimulus for TF-1 bioassays. The table summarizes typical workflow placement; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of IL-6\u003c\/th\u003e\n\u003cth\u003eCompanion signals and context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTF-1 bioassay\u003c\/td\u003e\n\u003ctd\u003eDose-dependent proliferation\u003c\/td\u003e\n\u003ctd\u003eDefined assay medium\u003c\/td\u003e\n\u003ctd\u003eProliferation and relative potency\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eB-cell study\u003c\/td\u003e\n\u003ctd\u003eDifferentiation support\u003c\/td\u003e\n\u003ctd\u003eActivation and companion cytokines\u003c\/td\u003e\n\u003ctd\u003eCD38, CD138, immunoglobulin secretion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHepatocyte response\u003c\/td\u003e\n\u003ctd\u003eAcute-phase signaling\u003c\/td\u003e\n\u003ctd\u003eTime course and receptor context\u003c\/td\u003e\n\u003ctd\u003epSTAT3, CRP, SAA genes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMyeloid\/DC workflow\u003c\/td\u003e\n\u003ctd\u003eMaturation-associated signal\u003c\/td\u003e\n\u003ctd\u003eGM-CSF\/IL-4 and maturation cocktail\u003c\/td\u003e\n\u003ctd\u003ePhenotype, cytokines, antigen presentation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eConfirm the responsive cell stage and receptor context, including membrane IL-6R versus soluble IL-6R.\u003c\/li\u003e\n\u003cli\u003eUse a published or internally validated protocol as the starting point and optimize the dose range in the chosen model.\u003c\/li\u003e\n\u003cli\u003eControl timing, medium, matrix and companion factors, and include vehicle\/no-factor and pathway-response controls.\u003c\/li\u003e\n\u003cli\u003eMeasure phenotype and function rather than cell number alone. No EC50 or universal working concentration is published.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eInterleukin-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eInterleukin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e26–30 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of human TF-1 cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Signaling Studies, Cell Proliferation, Hematopoietic Differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is evaluated by stimulation of human TF-1 cell proliferation.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eYasukawa K, Hirano T, Watanabe Y, et al. (1987) Structure and expression of human B cell stimulatory factor-2 (BSF-2\/IL-6) gene. EMBO J 6:2939-2945. \u003ca href=\"https:\/\/doi.org\/10.1002\/j.1460-2075.1987.tb02598.x\" target=\"_blank\" rel=\"noopener\"\u003e10.1002\/j.1460-2075.1987.tb02598.x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eTanaka T, Narazaki M, Kishimoto T (2014) IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol 6:a016295. \u003ca href=\"https:\/\/doi.org\/10.1101\/cshperspect.a016295\" target=\"_blank\" rel=\"noopener\"\u003e10.1101\/cshperspect.a016295\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eHunter CA, Jones SA (2015) IL-6 as a keystone cytokine in health and disease. Nat Immunol 16:448-457. \u003ca href=\"https:\/\/doi.org\/10.1038\/ni.3153\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/ni.3153\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641423429997,"sku":"HIM-I6-0010","price":108.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641434308973,"sku":"HIM-I6-0100","price":809.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL6_3-500x500.png?v=1791572848"},{"product_id":"recombinant-human-il-7-protein-bhp22200028","title":"Recombinant Human IL-7 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Hematopoietic Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-7 Protein\u003c\/strong\u003e is a tag-free, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-7 is used in research on hematopoiesis and lymphoid development, on the development and maintenance of T and B cells, and for culture and expansion of T cells, including expansion for adoptive cell therapy research, as well as in cancer and antiviral immunology research and studies of IL-7 as a vaccine adjuvant. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-7 is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. It drives multipotent (pluripotent) hematopoietic stem cells toward lymphoid progenitor cells, and it has been studied in immune reconstitution after allogeneic stem cell transplantation.\u003c\/p\u003e\n\u003cp\u003eThe recombinant human protein is a glycosylated monomer that appears at 9, 25 and 30 kDa under reducing conditions.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-7 is used mainly in hematopoiesis, immunology and T-cell research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHematopoiesis: proliferation and differentiation of hematopoietic stem cells into lymphoid progenitor cells.\u003c\/li\u003e\n\u003cli\u003eImmunology: development and maintenance of T cells and B cells.\u003c\/li\u003e\n\u003cli\u003eT-cell culture: culture and expansion of T cells, including expansion for adoptive cell therapy research.\u003c\/li\u003e\n\u003cli\u003eImmunotherapy research: IL-7 in anti-tumor and antiviral immune responses.\u003c\/li\u003e\n\u003cli\u003eVaccinology: studies of IL-7 as a vaccine adjuvant.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eHematopoietic growth factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e9–30 kDa (9, 25 and 30 kDa under reducing conditions). Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of murine 2E8 cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, Cell Proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of murine 2E8 cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSnyder KM, Mackall CL, Fry TJ (2006) IL-7 in allogeneic transplant: clinical promise and potential pitfalls. Leuk Lymphoma 47:1222-1228. \u003ca href=\"https:\/\/doi.org\/10.1080\/10428190600555876\" target=\"_blank\" rel=\"noopener\"\u003e10.1080\/10428190600555876\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eHeufler C, Topar G, Grasseger A, et al. (1993) Interleukin 7 is produced by murine and human keratinocytes. J Exp Med 178:1109-1114. \u003ca href=\"https:\/\/doi.org\/10.1084\/jem.178.3.1109\" target=\"_blank\" rel=\"noopener\"\u003e10.1084\/jem.178.3.1109\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641423495533,"sku":"HCT-I7-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641434374509,"sku":"HCT-I7-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL7-500x500.png?v=1791572855"},{"product_id":"recombinant-human-il-10-protein-bhp22200029","title":"Recombinant Human IL-10 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · IL-10 Family\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-10 Protein\u003c\/strong\u003e is a tag-free, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-10 is a strong anti-inflammatory cytokine used to study immune regulation and inflammation, including models of inflammatory bowel disease, rheumatoid arthritis and transplant tolerance, and in cancer immunology research. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterleukin 10 (IL-10) belongs to the IL-10 family of α-helical cytokines. This immunosuppressive cytokine is produced by macrophages, monocytes, T cells, B cells, keratinocytes and other mammalian cell types.\u003c\/p\u003e\n\u003cp\u003eIL-10 suppresses expression of several proinflammatory cytokines, such as IFN-γ, IL-2, IL-3, TNF and GM-CSF. Like IL-4, it strengthens humoral immune responses while dampening cell-mediated immune reactions.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-10 is used mainly in research on immune regulation and inflammation; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunomodulation: IL-10 as an anti-inflammatory cytokine that suppresses the immune response.\u003c\/li\u003e\n\u003cli\u003eAutoimmune disease research: models of inflammatory bowel disease and rheumatoid arthritis.\u003c\/li\u003e\n\u003cli\u003eTransplantation research: models of transplant tolerance.\u003c\/li\u003e\n\u003cli\u003eCancer immunology research: IL-10 in immune regulation of tumors.\u003c\/li\u003e\n\u003cli\u003eBasic research: regulation of the immune response and inflammation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eInterleukin 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eIL-10 family of α-helical cytokines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e17 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of MC\/9-2 cell proliferation (mouse mast cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of MC\/9-2 cells, a mouse mast cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eGesser B, Leffers H, Jinquan T, et al. (1997) Identification of functional domains on human interleukin 10. Proc Natl Acad Sci U S A 94:14620-14625. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.94.26.14620\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.94.26.14620\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSabat R, Grütz G, Warszawska K, et al. (2010) Biology of interleukin-10. Cytokine Growth Factor Rev 21:331-344. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.cytogfr.2010.09.002\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.cytogfr.2010.09.002\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641423692141,"sku":"HCT-I10-0010","price":215.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641434702189,"sku":"HCT-I10-0100","price":1339.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL10-500x500.png?v=1791572859"},{"product_id":"recombinant-human-il-12-protein-bhp22200030","title":"Recombinant Human IL-12 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Interleukin\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-12 Protein\u003c\/strong\u003e is a tag-free, glycosylated heterodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-12 is a strong immunostimulatory cytokine used in cancer immunotherapy and vaccine-adjuvant research and to study immune responses and signaling pathways, particularly T-cell activation and differentiation. Publications cited by the manufacturer cover immunotherapy, T cells, NK cells, DC-CIK and CAR-T cell expansion. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-12 (p70) consists of two disulfide-linked subunits, p35 and p40, each encoded by its own gene. It is produced mainly by macrophages and B lymphocytes.\u003c\/p\u003e\n\u003cp\u003eIL-12 acts as a growth factor for activated T and NK cells, increases the lytic activity of NK and lymphokine-activated killer (LAK) cells, and stimulates IFN-γ production by resting PBMC.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-12 is used mainly in immunotherapy and immune-response research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunotherapy research: IL-12 in anti-tumor and antiviral immune responses.\u003c\/li\u003e\n\u003cli\u003eCancer immunology research: IL-12-driven immune responses against tumor cells.\u003c\/li\u003e\n\u003cli\u003eVaccine research: studies of IL-12 as a vaccine adjuvant.\u003c\/li\u003e\n\u003cli\u003eBasic research: immune responses and cell signaling, particularly T-cell activation and differentiation.\u003c\/li\u003e\n\u003cli\u003eImmune-cell expansion: cited publications cover T cells, NK cells, DC-CIK and CAR-T cell expansion.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-12 (p70)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated heterodimer (p35 and p40 subunits)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eHeterodimeric interleukin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e57 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of PHA-transformed human peripheral blood lymphoblast proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, T Cell Activation Assay, Cell Proliferation, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of PHA-transformed human peripheral blood lymphoblasts.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eOppmann B, Lesley R, Blom B, et al. (2000) Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 13:715-725. \u003ca href=\"https:\/\/doi.org\/10.1016\/s1074-7613(00)00070-4\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/s1074-7613(00)00070-4\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGubler U, Chua AO, Schoenhaut DS, et al. (1991) Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc Natl Acad Sci U S A 88:4143-4147. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.88.10.4143\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.88.10.4143\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641423855981,"sku":"HCT-I12-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641435029869,"sku":"HCT-I12-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL12-500x500.png?v=1791572862"},{"product_id":"recombinant-human-il-15-protein-bhp22200031","title":"Recombinant Human IL-15 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Four α-Helix Bundle Cytokine\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-15 Protein\u003c\/strong\u003e is a tag-free, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-15 is a strong immunomodulatory cytokine used to expand and differentiate immune cells such as T cells and NK cells, and in research on cancer immunotherapy, immune responses to viral infection, vaccine adjuvants and immune modulation in autoimmune disease. Publications cited by the manufacturer cover immunotherapy, T cells, NK cells, DC-CIK and CAR-T cell expansion. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterleukin-15 (IL-15) is a T-cell growth factor of the four α-helix bundle cytokine family, which also includes G-CSF, GM-CSF, IL-2, IL-4, IL-7 and IL-9.\u003c\/p\u003e\n\u003cp\u003eIL-15 stimulates proliferation and activation of natural killer (NK) cells and T cells, and the number of CD8+ memory T cells is controlled by a balance between IL-15 and IL-2. It has an important role in both innate and adaptive immunity.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-15 is used mainly in immunotherapy and immune-cell research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunotherapy research: IL-15 in anti-tumor and antiviral immune responses.\u003c\/li\u003e\n\u003cli\u003eImmune-cell expansion: expansion and differentiation of T cells and NK cells.\u003c\/li\u003e\n\u003cli\u003eVaccinology: studies of IL-15 as a vaccine adjuvant.\u003c\/li\u003e\n\u003cli\u003eAutoimmune disease research: modulation of the immune response in autoimmune disease.\u003c\/li\u003e\n\u003cli\u003eBasic research: immune-cell development and function.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eInterleukin-15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eFour α-helix bundle cytokine family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e14–15 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of CTLL-2 cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Proliferation, T Cell Activation Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of CTLL-2 cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSteel JC, Waldmann TA, Morris JC (2012) Interleukin-15 biology and its therapeutic implications in cancer. Trends Pharmacol Sci 33:35-41. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.tips.2011.09.004\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.tips.2011.09.004\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eDi Sabatino A, Calarota SA, Vidali F, et al. (2011) Role of IL-15 in immune-mediated and infectious diseases. Cytokine Growth Factor Rev 22:19-33. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.cytogfr.2010.09.003\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.cytogfr.2010.09.003\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641423954285,"sku":"HCT-I15-0010","price":134.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641435128173,"sku":"HCT-I15-0100","price":1072.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL15-500x500.png?v=1791572867"},{"product_id":"recombinant-human-il-17-il-17a-protein-bhp22200032","title":"Recombinant Human IL-17 (IL-17A) Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · IL-17 Family\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-17 (IL-17A) Protein\u003c\/strong\u003e is a tag-free, glycosylated protein expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-17A is a strong pro-inflammatory cytokine used to study inflammation and immune responses, the development of autoimmune diseases such as rheumatoid arthritis and psoriasis, and its effects on tumor growth and metastasis. It is also used in drug-discovery research on inflammatory disease. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-17 is a family of structurally related cytokines that share a highly conserved C-terminal region but differ in their N-terminal regions and in their biological roles. IL-17 (IL-17A) is secreted by activated T cells, including Th17 cells, as a homodimer of two 136-amino-acid chains.\u003c\/p\u003e\n\u003cp\u003eIL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines and increases surface expression of ICAM1 (intercellular adhesion molecule 1) on fibroblasts.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-17A is used mainly in inflammation, autoimmunity and cancer research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInflammation: IL-17A as a strong pro-inflammatory cytokine in the inflammatory response.\u003c\/li\u003e\n\u003cli\u003eAutoimmune disease: development of rheumatoid arthritis, psoriasis and related conditions.\u003c\/li\u003e\n\u003cli\u003eCancer research: effects of IL-17A on tumor growth and metastasis.\u003c\/li\u003e\n\u003cli\u003eDrug discovery: IL-17A as a target in inflammatory disease research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-17A (also known as IL-17)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eIL-17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated (manufacturer lists a monomer; the description states a homodimer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eIL-17 family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e30–35 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of IL-6 expression in NHDF adult fibroblasts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to induce IL-6 expression in NHDF adult fibroblasts.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBettelli E, Korn T, Oukka M, et al. (2008) Induction and effector functions of T(H)17 cells. Nature 453:1051-1057. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature07036\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature07036\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eCua DJ, Tato CM (2010) Innate IL-17-producing cells: the sentinels of the immune system. Nat Rev Immunol 10:479-489. \u003ca href=\"https:\/\/doi.org\/10.1038\/nri2800\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nri2800\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFossiez F, Djossou O, Chomarat P, et al. (1996) T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines. J Exp Med 183:2593-2603. \u003ca href=\"https:\/\/doi.org\/10.1084\/jem.183.6.2593\" target=\"_blank\" rel=\"noopener\"\u003e10.1084\/jem.183.6.2593\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641424118125,"sku":"HIM-I17a-0010","price":85.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641435586925,"sku":"HIM-I17a-0100","price":747.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL17-500x500.png?v=1791572871"},{"product_id":"recombinant-human-il-21-protein-bhp22200033","title":"Recombinant Human IL-21 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Interleukin\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-21 Protein\u003c\/strong\u003e is a tag-free, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-21 is a strong immunomodulatory cytokine used in research on immune responses and cell signaling, cancer immunotherapy, immune responses to viral infection, vaccine adjuvants and immune modulation in autoimmune disease. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-21 is a typical family I cytokine with broad pleiotropic effects. It is produced mainly by T follicular helper cells, Th17 cells and natural killer T cells after antigen stimulation. IL-21 generally strengthens antigen-specific responses by controlling activation, proliferation, differentiation, cytotoxicity and survival of its target immune cells, and it promotes the anti-tumor activity of CD8+ T cells and NK cells.\u003c\/p\u003e\n\u003cp\u003eIL-21 acts through IL-21R, a receptor that also contains the common gamma chain found in the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. Binding activates the tyrosine kinases JAK1 and JAK3, followed by the transcription factors STAT1 and STAT3. The recombinant human protein is described as a 15.4 kDa protein of 132 amino acid residues.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-21 is used mainly in immunotherapy and immune-signaling research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunotherapy research: IL-21 in anti-tumor and antiviral immune responses.\u003c\/li\u003e\n\u003cli\u003eCancer immunology research: anti-tumor activity of CD8+ T cells and NK cells.\u003c\/li\u003e\n\u003cli\u003eAutoimmune disease research: immune modulation in autoimmune disease.\u003c\/li\u003e\n\u003cli\u003eVaccinology: studies of IL-21 as a vaccine adjuvant.\u003c\/li\u003e\n\u003cli\u003eBasic research: immune responses and cell signaling pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eFamily I cytokine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e~15 kDa (15.4 kDa, 132 amino acid residues). Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eCell proliferation assay using human N1186 T cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eED50\u003c\/th\u003e\n\u003ctd\u003eAbout 0.5–5 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured in a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5–5 ng\/mL.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eParrish-Novak J, Dillon SR, Nelson A, et al. (2000) Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature 408:57-63. \u003ca href=\"https:\/\/doi.org\/10.1038\/35040504\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/35040504\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSpolski R, Leonard WJ (2008) Interleukin-21: basic biology and implications for cancer and autoimmunity. Annu Rev Immunol 26:57-79. \u003ca href=\"https:\/\/doi.org\/10.1146\/annurev.immunol.26.021607.090316\" target=\"_blank\" rel=\"noopener\"\u003e10.1146\/annurev.immunol.26.021607.090316\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMehta DS, Wurster AL, Grusby MJ (2004) Biology of IL-21 and the IL-21 receptor. Immunol Rev 202:84-95. \u003ca href=\"https:\/\/doi.org\/10.1111\/j.0105-2896.2004.00201.x\" target=\"_blank\" rel=\"noopener\"\u003e10.1111\/j.0105-2896.2004.00201.x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641424511341,"sku":"HCT-I21-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641435750765,"sku":"HCT-I21-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL21_1-500x500.png?v=1791572875"},{"product_id":"recombinant-human-il-23-protein-bhp22200034","title":"Recombinant Human IL-23 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Interleukin\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-23 Protein\u003c\/strong\u003e is a tag-free, glycosylated heterodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-23 is used in immunology research on the regulation of immune responses, particularly T-cell differentiation and activation, in research on autoimmune diseases such as psoriasis and inflammatory bowel disease, and in studies of its influence on tumor growth and metastasis. It is also used in drug-discovery research on autoimmune disease. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-23 is a heterodimeric cytokine made of a unique p19 subunit and a p40 subunit that it shares with IL-12. It is secreted by activated dendritic cells and macrophages.\u003c\/p\u003e\n\u003cp\u003eIL-23 binds a heterodimeric receptor complex of IL12RB1 and IL23R and activates the JAK-STAT signaling cascade. It acts mainly on memory T cells rather than naive T cells and increases production of proinflammatory cytokines such as IFNγ and IL-17. Through these effects IL-23 drives autoimmune inflammation, may contribute to autoimmune inflammatory diseases and may be important in tumorigenesis.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-23 is used mainly in immunology and autoimmune-disease research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eImmunology research: regulation of immune responses, particularly T-cell differentiation and activation.\u003c\/li\u003e\n\u003cli\u003eAutoimmune disease research: the role of IL-23 in psoriasis and inflammatory bowel disease.\u003c\/li\u003e\n\u003cli\u003eCancer research: influence of IL-23 on tumor growth and metastasis.\u003c\/li\u003e\n\u003cli\u003eDrug discovery: IL-23 as a target in autoimmune disease research.\u003c\/li\u003e\n\u003cli\u003eBasic research: mechanisms of immune response, inflammation and autoimmunity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-23\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated heterodimer (p19 and p40 subunits)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eHeterodimeric interleukin (p40 subunit shared with IL-12)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e55 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of IL-17 expression in murine splenocytes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, T Cell Activation Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to induce IL-17 expression in murine splenocytes.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eOppmann B, Lesley R, Blom B, et al. (2000) Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 13:715-725. \u003ca href=\"https:\/\/doi.org\/10.1016\/s1074-7613(00)00070-4\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/s1074-7613(00)00070-4\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eLupardus PJ, Garcia KC (2008) The structure of interleukin-23 reveals the molecular basis of p40 subunit sharing with interleukin-12. J Mol Biol 382:931-941. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.jmb.2008.07.051\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.jmb.2008.07.051\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eAggarwal S, Ghilardi N, Xie MH, et al. (2003) Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17. J Biol Chem 278:1910-1914. \u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.M207577200\" target=\"_blank\" rel=\"noopener\"\u003e10.1074\/jbc.M207577200\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641424806253,"sku":"HCT-I23-0010","price":143.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641436963181,"sku":"HCT-I23-0100","price":1035.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL23-500x500.png?v=1791572879"},{"product_id":"recombinant-human-il-28a-protein-bhp22200035","title":"Recombinant Human IL-28A Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Type III Interferon\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-28A Protein\u003c\/strong\u003e is a tag-free, non-glycosylated homodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-28A (IFN-λ2) is a strong antiviral cytokine used to study antiviral immunity and immune regulation, including antiviral studies with hepatitis viruses such as hepatitis C virus (HCV) and hepatitis B virus (HBV), immune modulation and antitumor activity research. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-28A, also called interferon λ2 (IFNλ2), belongs to the type III interferon family, which is distinct from the type I IFNs. Its expression is induced by viral infection or double-stranded RNA.\u003c\/p\u003e\n\u003cp\u003eIL-28A is a ligand for the heterodimeric class II cytokine receptor formed by IL10RB and IFNLR1. Receptor engagement activates JAK\/STAT signaling and the expression of IFN-stimulated genes (ISGs), which establish the antiviral state. IL-28A has antiviral, antitumor and immunomodulatory activities and plays a key role in antiviral host defense, mainly in epithelial tissues.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-28A is used mainly in antiviral-immunity and immune-regulation research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAntiviral activity: inhibition of virus replication in studies with hepatitis C virus (HCV) and hepatitis B virus (HBV).\u003c\/li\u003e\n\u003cli\u003eImmunomodulation: modulation of the immune response, with possible relevance to autoimmune disease.\u003c\/li\u003e\n\u003cli\u003eAntitumor activity research: antitumor effects of type III interferons.\u003c\/li\u003e\n\u003cli\u003eBasic research: antiviral immunity and immune regulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-28A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eIFN-λ2; Interferon λ2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eType III interferon family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e24 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eAntiviral activity in A549 cells (human lung adenocarcinoma epithelial cell line) infected with encephalomyocarditis (EMC) virus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Virus studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the antiviral effect of the protein in A549 cells (a human lung adenocarcinoma epithelial cell line) infected with encephalomyocarditis (EMC) virus.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eDonnelly RP, Kotenko SV (2010) Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res 30:555-564. \u003ca href=\"https:\/\/doi.org\/10.1089\/jir.2010.0078\" target=\"_blank\" rel=\"noopener\"\u003e10.1089\/jir.2010.0078\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSheppard P, Kindsvogel W, Xu W, et al. (2003) IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat Immunol 4:63-68. \u003ca href=\"https:\/\/doi.org\/10.1038\/ni873\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/ni873\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641424970093,"sku":"HIN-I28a-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641437127021,"sku":"HIN-I28a-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL28A-500x500.png?v=1791572883"},{"product_id":"recombinant-human-il-29-protein-bhp22200036","title":"Recombinant Human IL-29 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Type III Interferon\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human IL-29 Protein\u003c\/strong\u003e is a tag-free, non-glycosylated homodimer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eIL-29 (IFN-λ1) is a strong antiviral cytokine used to study antiviral immunity and immune regulation, including antiviral studies with hepatitis viruses such as hepatitis C virus (HCV) and hepatitis B virus (HBV), immune modulation and antitumor activity research. The manufacturer expresses this human protein in human cells, with extremely low endotoxin and high purity.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eIL-29 belongs to the type III interferon family, which is distinct from the type I IFNs. Its expression is induced by viral infection or double-stranded RNA.\u003c\/p\u003e\n\u003cp\u003eIL-29 is a ligand for the heterodimeric class II cytokine receptor formed by IL10RB and IFNLR1. Receptor engagement activates JAK\/STAT signaling and the expression of IFN-stimulated genes (ISGs), which establish the antiviral state. IL-29 has antiviral, antitumour and immunomodulatory activities.\u003c\/p\u003e\n\u003cp\u003eIL-29 acts mainly on epithelial cells, because its receptor subunit IFNLR1 is expressed specifically on that cell type. It also has an immunomodulatory effect through up-regulation of MHC class I antigen expression.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eIL-29 is used mainly in antiviral-immunity and immune-regulation research; the main research areas are listed below.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAntiviral activity: inhibition of virus replication in studies with hepatitis C virus (HCV) and hepatitis B virus (HBV).\u003c\/li\u003e\n\u003cli\u003eImmunomodulation: modulation of the immune response, with possible relevance to autoimmune disease.\u003c\/li\u003e\n\u003cli\u003eAntitumor activity research: antitumor effects of type III interferons.\u003c\/li\u003e\n\u003cli\u003eBasic research: antiviral immunity and immune regulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIL-29\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eIFN-λ1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eType III interferon family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e29–35 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eAntiviral activity in A549 cells (human lung adenocarcinoma epithelial cell line) infected with encephalomyocarditis (EMC) virus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Virus studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the antiviral effect of the protein in A549 cells (a human lung adenocarcinoma epithelial cell line) infected with encephalomyocarditis (EMC) virus.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted protein under sterile conditions at 2°C to 8°C for up to 1 month, or at −20°C to −80°C for up to 3 months.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSheppard P, Kindsvogel W, Xu W, et al. (2003) IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat Immunol 4:63-68. \u003ca href=\"https:\/\/doi.org\/10.1038\/ni873\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/ni873\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eDonnelly RP, Kotenko SV (2010) Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res 30:555-564. \u003ca href=\"https:\/\/doi.org\/10.1089\/jir.2010.0078\" target=\"_blank\" rel=\"noopener\"\u003e10.1089\/jir.2010.0078\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKotenko SV, Gallagher G, Baurin VV, et al. (2003) IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex. Nat Immunol 4:69-77. \u003ca href=\"https:\/\/doi.org\/10.1038\/ni875\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/ni875\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641425035629,"sku":"HIN-I29-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641437618541,"sku":"HIN-I29-0100","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IL29-500x500.png?v=1791572887"}],"url":"https:\/\/www.ebiohippo.com\/collections\/stemimmune.oembed","provider":"BioHippo","version":"1.0","type":"link"}