{"title":"Trophoblast Cell Lines","description":"\u003cp\u003eHuman trophoblast and placental cell lines for studying placentation, syncytialization, trophoblast invasion, and placental disease. Includes the most widely used choriocarcinoma and trophoblast models — BeWo, JEG-3, JAR, and HTR-8\/SVneo — the hTERT-immortalized Swan-71 line, engineered Cas9 and CRISPR-knockout derivatives for functional studies, and primary human villous trophoblasts. Use these models alongside organoid culture and placental marker ELISA kits in our Placental \u0026amp; Trophoblast Research collection.\u003c\/p\u003e","products":[{"product_id":"bewo-cell-bhc11100033","title":"BEWO cell","description":"BeWo cells, a cell line derived from malignant gestational choriocarcinoma of the fetal male placenta, have become a widely used in vitro model for studying the placenta. \nThe cell-cell fusion during the human trophoblast syncytialization phase during placental development is one of the most significant yet least understood events. Due to the difficulty of studying this process in a placenta in vivo, BeWo cells are utilized as a cell culture model to simulate in vivo syncytialization of the placental villous trophoblast.\nThese cells exhibit an epithelial-like phenotype and are adherent. The b30 subclone of BeWo cells is particularly useful for studying nutrient uptake and transport due to its dense growth on permeable membranes.\nCK 7 and E-cadherin are molecular markers that are expressed by BeWo cells. VE-cadherin is found in BeWo cells and is enhanced upon treatment with forskolin. The cells also express keratin and are positive for G6PD, B isoenzyme. The karyotype of BeWo cells is modal number = 86, with a range of 71 to 178, and the stemline number is hypotetraploid. \nThe karyotype is relatively stable within the stemline number. BeWo cells secrete various hormones, including human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen), and steroid hormones as estrone, estriol, and estradiol. \nHowever, the levels of β-hCG and estradiol secreted by BeWo cells are lower than those secreted by other choriocarcinoma-derived cell lines such as JEG-3. Upon Forskolin treatment, the secretion of β-hCG in BeWo cells increases to a level similar to that observed in the other choriocarcinoma-derived cell lines. Furthermore, Forskolin treatment also increases the progesterone levels secreted by BeWo cells.\nIn summary, BeWo cells are a widely used in vitro model for studying placental development and the human trophoblast syncytialization process. They exhibit an epithelial-like phenotype, express various molecular markers, and secrete multiple hormones, including hCG, placental lactogen, and steroid hormones. Overall, BeWo cells are a valuable tool for investigating the complex processes involved in placental development.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100033\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950198157677,"sku":"300123","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bewo-_281_29_1920x1920_f975a6b5-8edd-4f11-8b59-cda5f21e8d0b.jpg?v=1769068955"},{"product_id":"htr-8-svneo-cell-bhc11101405","title":"HTR-8\/SVneo cell","description":"HTR-8\/SVneo is a human trophoblast cell line derived from the chorionic villi of a first-trimester placenta, specifically from a 6-to-12-week-old embryo. These cells were immortalized by transfecting them with the gene encoding the simian virus 40 (SV40) large T antigen, which extends their lifespan while maintaining characteristics typical of extravillous invasive trophoblasts. This cell line expresses several key markers associated with extravillous trophoblasts, including insulin-like growth factor II (IGF-II), NDOG-5, proliferating cell nuclear antigen (PCNA), and a range of integrins (α1, α3, α5, αv, and β1 subunits, along with the αvβ3\/β5 vitronectin receptor). It is negative for macrophage marker 63\/D3, endothelial cell marker factor VIII, and α6 and β4 integrin subunits, confirming its trophoblast lineage and distinguishing it from other cell types such as macrophages and endothelial cells.\n\nHTR-8\/SVneo cells are widely used as a model to study trophoblast invasion and placental biology, particularly the epithelial-to-mesenchymal transition (EMT), which is crucial for trophoblasts' invasive behavior during placental development. Research has shown that these cells exhibit a mixed population of epithelial and mesenchymal phenotypes, with the ability to undergo EMT under standard culture conditions. This transition is mediated by TGF-β signaling, which promotes the mesenchymal phenotype, as evidenced by the upregulation of mesenchymal markers such as vimentin and the downregulation of epithelial markers like E-cadherin. This makes HTR-8\/SVneo a valuable in vitro model for studying the molecular mechanisms underlying EMT in trophoblasts and its implications in both normal placental development and pregnancy-related disorders.\n\nStudies have further demonstrated that HTR-8\/SVneo cells can form spheroids, which predominantly express epithelial markers. When these spheroids are re-plated in 2D culture, the cells exhibit a shift towards a mesenchymal phenotype, indicating an ongoing EMT process. This cell line's unique properties, including its responsiveness to TGF-β and its mixed epithelial-mesenchymal nature, provide critical insights into the complex cellular dynamics of trophoblast invasion and the regulation of placental development, offering a robust platform for investigating pregnancy-related pathologies such as pre-eclampsia and intrauterine growth restriction.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101405\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950212116845,"sku":"305221","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HTR-8SVneo-_281_29_1920x1920_6964aa39-58ff-4a93-a458-3bc800305f22.jpg?v=1769069109"},{"product_id":"jar-cell-bhc11100379","title":"JAR cell","description":"The JAR cell line is a human choriocarcinoma cell line derived from trophoblastic cells of placental origin. This cell line is widely utilized in cancer research, particularly in studies related to gestational trophoblastic diseases and placental development. JAR cells exhibit characteristics typical of choriocarcinoma, including high levels of human chorionic gonadotropin (hCG) production, which makes them a valuable model for studying hormone regulation, placental biology, and the mechanisms underlying trophoblastic tumorigenesis.\n\nJAR cells are known for their invasive properties and ability to proliferate rapidly, which mirrors the aggressive nature of choriocarcinomas in vivo. These cells are also used to investigate the interaction between trophoblastic cells and the maternal immune system, providing insights into immune evasion mechanisms. Additionally, JAR cells have been employed in studies of drug resistance and chemosensitivity, aiding in the development of therapeutic strategies against trophoblastic cancers. As a cell line derived from human tumors, JAR cells are strictly for in vitro research and are not suitable for any in vivo or therapeutic applications.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100379\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950212837741,"sku":"300221","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/JAR_20P1_2020x01_20150825_ch00_1920x1920_d8eda7a1-4a82-4d8a-af4f-3a975334786a.png?v=1769069117"},{"product_id":"jeg-3-cell-bhc11100617","title":"JEG-3 cell","description":"The JEG-3 cell line is derived from a human choriocarcinoma, a type of cancer that originates from trophoblastic cells in the placenta. These cells exhibit properties characteristic of trophoblasts, including the ability to produce hormones such as human chorionic gonadotropin (hCG), which is crucial for pregnancy maintenance. JEG-3 cells are epithelial in nature and are often utilized in research focused on placental function, cancer biology, and endocrine signaling.\n\nJEG-3 cells are known for their aggressive growth characteristics and capacity to invade surrounding tissues, making them a valuable model for studying the mechanisms of trophoblastic tumor invasion and metastasis. Additionally, they have been used extensively in research investigating the molecular pathways involved in placental development, as well as the role of trophoblasts in immune tolerance during pregnancy. The cells are typically cultured in RPMI-1640 medium supplemented with fetal bovine serum and other growth factors to support their proliferation and maintenance.\n\nThis cell line provides a robust platform for investigating placental cancer biology, hormone production, and the interaction between trophoblasts and the maternal immune system.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100617\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950212870509,"sku":"300222","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/JEG-3_20P1_2020x01_20060625_ch00_1920x1920_072bb223-52c8-4b67-912b-b99ce103cbbb.jpg?v=1769069116"},{"product_id":"immortalized-human-trophoblast-cells-negative-htert-sw-71-bhc10900690","title":"Immortalized Human Trophoblast Cells - hTERT (Sw.71)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Human Trophoblast Cells - hTERT (Sw.71) were derived from 7-week first trimester placenta isolated from normal pregnancy and were virally infected with hTERT. The immortalized cells retained the same morphology as their primary counterparts.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Trophoblast Cells - hTERT (Sw.71) is supplied as an immortalized cell line derived from Human placenta.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 10% FBS(Regular*) + 1 mM Sodium Pyruvate (TM057) + 2 mM L-glutamine (G275) + 10 mM HEPES (TM058) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIt has the abilities to spontaneously fuse as occasional multi-nucleated cells clusters may form, and can secrete cytokines (IL-1β, IL-6, IL-8, Monocyte Chemotactic Protein-1 (MCP-1), GRO (CXCR2), GRO-α (CXCL1), Intercellular Adhesion Molecule-1 (ICAM-1), Osteoprotegerin (OPG), Tissue Inhibitor of Metalloproteinase-1 (TIMP-1), TIMP-2 and VEGF). Cytokeratin 7 and vimentin markers are expressed. The population of cells is determined to be free of immune cells due to the absence of CD45 and CD68, and free of fibroblast cells due to the lack of fibroblast specific antigen. The Sw.71 cells are a valuable tool for trophoblast research. Donor\/background information provided for this product: 7 weeks.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 10% FBS(Regular*) + 1 mM Sodium Pyruvate (TM057) + 2 mM L-glutamine (G275) + 10 mM HEPES (TM058) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e3D Culture Conditions:\u003c\/strong\u003e Procedure Preparation (before 3D Culture): Thaw cells following \"Thawing Protocol\" and \"Growth Conditions\". Expand cells for one passage, then seed for spheroid generation (3D culture). Subculture Protocol: Aspirate the supernatant and wash cells with 1X PBS (pH 7.4) (G5000). Dissociate cells using 1–1.5 ml of Gentle Dissociation Solution (TM080). Observe the cells under a microscope to confirm detachment (typically within 2–10 minutes). Cells that are difficult to detach can be put in 37 °C for several minutes to facilitate detachment. Neutralize using an equal volume of complete growth media (must contain serum) or using Trypsin Neutralizing Solution (TM069). Transfer culture suspension into sterile conical centrifuge tube (G5500), and centrifuge at 125 x g for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. Aspirate supernatant and re-suspend the pellet with pre-warmed fresh complete growth media for 3D Culture. Spheroid Generation Protocol: Count cells and viability using cell counter or hemocytometer with Trypan Blue Stain (TM071). Proceed if cells are \u0026gt;90% viability. Seed 500 to 10,000 cells per well in SpheroWell™ 96 Well Plate (G7540), final volume maintained at 100 μl per well. Avoid cell clumps (clumps will not generate uniform spheroids). Count as Day 0. Tip: Fill the outermost wells of the plate with 1X PBS (pH 7.4) (G5000) to prevent evaporation of media during incubation. (Recommended) Centrifuge the sealed plate at 300 x g for 5 minutes. Place in the incubator set at 37 °C, 5% CO₂. Change Media: Every 2–3 days, add 100 μl of fresh media slowly along the wall of the well to avoid disrupting spheroids at the bottom. Take out 100 μl of media slowly and discard from the well. (Recommended, but optional) Centrifuge the sealed plate at 300 x g for 5 minutes. Monitor the Cells for 3D\/Spheroid Formation: Spheroid formation will appear between day 5 and 10.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180505915757,"sku":"T0532","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/uTEgl29Wp07UXjfkZmVVwqgQ7wTGJmjjcuPfYTLg.png?v=1782151788"},{"product_id":"jeg-3-cells-bhc10901799","title":"JEG-3 Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eJEG-3 Cells is a tumor cell line supplied in frozen format and associated with Human placenta biology.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e JEG-3 Cells is supplied as a tumor cell line derived from Human placenta.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in neurobiology, differentiation, and cell signaling. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Male, Fetus, Choriocarcinoma (metastatic site, brain).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:4 to 1:6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 20,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180537700717,"sku":"T8976","price":320.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/wZeM1EDJqWoDmiML55RKihzAoU8Wpz4LU4up30x3.png?v=1774957847"},{"product_id":"cas9-expressing-jeg-3-cell-line-bhc10923141","title":"Cas9 Expressing JEG-3 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line stably expresses spCas9.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Cas9 Expressing JEG-3 Cell Line is supplied as an engineered cell line derived from Human placenta.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 0.2 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in placenta biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in placenta biology, phenotype comparison, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Choriocarcinoma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 0.2 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:4 or 1:6\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180551266669,"sku":"T3701","price":640.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/RycbyCneaCOrB8NplXnVQwDYZDa4Dh09bTO9aRoF.png?v=1774957882"},{"product_id":"rhot2-crispr-knockout-htr-8-svneo-stable-cell-line-t2-10-bhc10923355","title":"RHOT2 CRISPR Knockout HTR-8\/Svneo Stable Cell Line-T2-10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe RHOT2 CRISPR Knockout HTR-8\/Svneo Stable Cell Line – T2-10 is a human trophoblast model engineered for targeted disruption of the RHOT2 gene, a key regulator of mitochondrial transport and dynamics. This stable knockout line enables detailed studies of placental biology, mitochondrial function, and cellular signaling pathways.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e RHOT2 CRISPR Knockout HTR-8\/Svneo Stable Cell Line-T2-10 is supplied as an engineered cell line derived from Human placenta.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent , Epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate 0.6 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in placenta biology, phenotype comparison, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIts validated genotype and consistent growth make it a reliable platform for mechanistic research, and CRISPR-based functional assays.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate 0.6 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2-1:3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 24-48\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180554740077,"sku":"T7362","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IDy7ss1FEDYLO3WTey1TWJYJF8mlG7oPAKFwwQUw.png?v=1774957889"},{"product_id":"htr-8-svneo-cells-bhc10923472","title":"HTR-8\/SVneo Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eHTR-8\/SVneo Cells are a first-trimester human trophoblast cell line immortalized using the SV40 large T antigen, originally derived from chorionic villi of 6–12 week placentas. They express key markers of extravillous invasive trophoblasts, including IGF-II, PCNA, and a specific set of integrins, while lacking macrophage and endothelial markers.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e HTR-8\/SVneo Cells is supplied as a tumor cell line derived from Human placenta.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, Epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM, High Glucose Medium (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eWidely used in placental and trophoblast research, these cells provide a robust model for studying early placental biology and cellular invasion. Donor\/background information provided for this product: Female, 6-12 Gestational weeks.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM, High Glucose Medium (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2-1:3\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180557853037,"sku":"T9998","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/kJGHVxIBIgvZ21LkA2LOZV8QYEWWC8NAEXsLosSg.png?v=1774957897"},{"product_id":"human-villous-trophoblasts-hvt-bhc18500068","title":"Human Villous Trophoblasts (HVT)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Villous Trophoblasts (HVT)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Epithelial Cells derived from placenta (Villous Trophoblasts) within the Reproductive system.\u003c\/p\u003e\n\u003cp\u003eThe trophoblast begins at the outer covering of the early blastocyst and provides the route of nourishment between the maternal endometrium and the developing embryo. The trophoblast adhesion to the uterine wall is the requisite first step of implantation and, subsequently, placentation. Human villous tryophoblasts (HVT) covering the villi of the placenta provide the surface for the exchange of oxygen and nutrients with the maternal circulation. They synthesize and release chorionic gonadotropin, placental lactogen and angiogenin [1] and express CXCR4, CCR5 and prolactin gene family [2, 3] . They acquire CCR1 as they differentiate to an invasive phenotype at the villous-anchoring sites [4] . The features of HVT, together with the recent establishment of trophoblast stem cells, make them an ideal genetic platform to study cell differentiation and organogenesis. iXCells Biotechnologies provides high quality HVT, which are isolated from human placental villi and cryopreserved at P1, with \u0026gt;0.5 million cells in each vial. HVT express alpha-and beta-HCG and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung. HVT can further expand no more than 3 passages in Trophoblast Growth Medium ( Cat# MD-0058 ) under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the purity. Figure 1. Human Villous Trophoblasts (HVT) phase contrast image\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Epithelial Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e placenta; Villous Trophoblasts; Reproductive\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor background:\u003c\/strong\u003e Gender: Female\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEpithelial cells provide barrier and transport functions across tissues, coordinating innate defense, secretion, and repair responses in the face of environmental and inflammatory stressors.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eModel hormone and cytokine signaling relevant to reproductive tissue physiology\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500068\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197815349613,"sku":"10HU-214-0.5M","price":875.68,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 1 million cells\/vial","offer_id":53197819969901,"sku":"10HU-214-1M","price":1157.52,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-214-0.5M.png?v=1775378645"}],"url":"https:\/\/www.ebiohippo.com\/collections\/trophoblast-cell-lines.oembed","provider":"BioHippo","version":"1.0","type":"link"}