rAAV-EF1a-DIO-hChR2(H134R)-mCherry

BC-0108

EF1a

hChR2(H134R)

930

mCherry

708

AAV1; AAV5; AAV8; AAV9; AAV.PHP.eB; AAV2; AAV6; AAV.rh10; AAV.DJ; AAV.DJ8; AAV.PHP.B; AAV.PHP.S; AAV2/RETRO; AAV.anc80

AAV

This AAV expresses hChR2(H134R)-mCherry driven by an ubiquitous Ef1alpha promoter in Cre recombinase-dependent manner.
The 1.2 Kb EF1alpha promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1alpha). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished activity or have been silenced.
Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. The wild-type, as well as a few mutations, provide the fastest excitation of the channelrhodopsins offered, and are widely used in optogenetics techniques in neuroscience. Due to delayed channel closure, hChR2(H134R) is a gain-of-function mutation that produces larger photocurrents than wild-type hChR2, but slows down channel kinetics.
mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp., with a Excitation/Emission spectrum around 587nm/610nm. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
DIO is double-floxed inverse Open reading frame. In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5' promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be "FLip-EXchanged" or FLEXed, leading to expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO .

2×10¹² vg/ml-5×10¹² vg/ml
If you need a higher titer, please email us at [email protected]

Iodixanol gradient ultracentrifugation

Store at -80℃. Thaw just before use and keep on ice.