rAAV-hSyn-hChR2(H134R)-EYFP

BC-0346

hSyn

hChR2(H134R)

930

EYFP

720

AAV1; AAV5; AAV8; AAV9; AAV.PHP.eB; AAV2; AAV6; AAV.rh10; AAV.DJ; AAV.DJ8; AAV.PHP.B; AAV.PHP.S; AAV2/RETRO; AAV.anc80

This AAV expresses hChR2(H134R)-EYFP driven by a neuron hSyn promoter.
hSyn is a Human synapsin 1 gene promoter.
Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. The wild-type, as well as a few mutations, provide the fastest excitation of the channelrhodopsins offered, and are widely used in optogenetics techniques in neuroscience. Due to delayed channel closure, hChR2(H134R) is a gain-of-function mutation that produces larger photocurrents than wild-type hChR2, but slows down channel kinetics.
EYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria, with a Excitation/Emission spectrum around 513nm/527nm. It is reported to be a very rapidly-maturing weak dimer with high acid sensitivity.

2×10¹² vg/ml-5×10¹² vg/ml
If you need a higher titer, please email us at [email protected]

Iodixanol gradient ultracentrifugation

Store at -80℃. Thaw just before use and keep on ice.