Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6.
Homo Sapiens (Human)
Synthetic phosphopeptide derived from human Caspase 9 around the phosphorylation site of Threonine 125.
Human, Mouse, Rat
Product: 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.Specificity: Caspase-9 (phospho-T125) polyclonal antibody detects endogenous levels of Caspase 9 protein only when phosphorylated at Thr125.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.