Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Homo Sapiens (Human)
Synthetic peptide, corresponding to amino acids 411-460 of Human Vimentin.
Human, Mouse, Rat
40, 57 kDa
Product: 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.Specificity: Vimentin (I444) polyclonal antibody detects endogenous levels of Vimentin protein.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.