Mouse Anti-4-HydroXy-2-heXenal (4-HHE) Monoclonal IgG2b

Mfr.No.	SMC-510, SMC-510D, SMC-510D-A390, SMC-510D-A488, SMC-510D-A594, SMC-510D-ALP, SMC-510D-APC, SMC-510D-BI, SMC-510D-FITC, SMC-510D-HRP, SMC-510D-PCP, SMC-510D-RPE, SMC-510S
Alternative Names	4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody
Antigen	4-Hydroxy-2-hexenal
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-510 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 ug of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-4-Hydroxy-2-hexenal (4-HHE) Monoclonal IgG2b
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Region	Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Isotype	IgG2b
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	4-Hydroxy-2-hexenal
Clone No	6F10
Research Area	Cancer, Oxidative Stress, Lipid peroxidation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, FACS, WB, ICC/IF, FCM
Description	WB ; ICC ; IF ; ELISA ; FACS ; FCM
Dilution	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11905483-1	SMC-510D	Unconjugated	100 ug	$409	Add to Cart
BHA11905483-2	SMC-510D-A390	ATTO 390	100 ug	$463	Add to Cart
BHA11905483-3	SMC-510D-A488	ATTO 488	100 ug	$462	Add to Cart
BHA11905483-4	SMC-510D-A594	ATTO 594	100 ug	$462	Add to Cart
BHA11905483-5	SMC-510D-ALP	Alkaline Phosphatase	100 ug	$455	Add to Cart
BHA11905483-6	SMC-510D-APC	APC	100 ug	$461	Add to Cart
BHA11905483-7	SMC-510D-BI	Biotin	100 ug	$458	Add to Cart
BHA11905483-8	SMC-510D-FITC	FITC	100 ug	$453	Add to Cart
BHA11905483-9	SMC-510D-HRP	HRP	100 ug	$449	Add to Cart
BHA11905483-10	SMC-510D-PCP	PerCP	100 ug	$461	Add to Cart
BHA11905483-11	SMC-510D-RPE	RPE	100 ug	$459	Add to Cart
BHA11905483-12	SMC-510S	Unconjugated	12 ug	$44	Add to Cart

4-Hydroxy-2-hexenal Antibody, Clone 6F10 - SMC-510