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Home / Primary Antibodies / Monoclonal Antibodies

Alpha B Crystallin Antibody, Clone 1A7.D5 - SMC-159

BHA11900876
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+1 866.986.9598
Email
[email protected]
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Swissprot P02511
Accession Number NP_001876.1
Gene Id 1410
Target Alpha B Crystallin
Clone No 1A7.D5
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Neuroscience, Protein Trafficking
Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~20kDa (Predicted mol. weight is ~21kDa). Does not cross-react with alphaA-crystallin, betaL-crystallin, betaH-crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, WB, ICC/IF
Description WB ; ELISA ; ICC ; IF
Dilution WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.

2. Horwitz J. (1992) Proc Natl Acad Sci USA. 89(21): 10449-10453.

3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490

4. Horwitz J. (2003) Exp Eye Res. 76: 145-153.

5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.

6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.

7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215.

8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.

9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.

10. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900876-1 SMC-159B Unconjugated 200 ug $295
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BHA11900876-2 SMC-159A Unconjugated 50 ug $145
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BHA11900876-3 SMC-159B-A390 ATTO 390 200 ug $397
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BHA11900876-4 SMC-159B-A488 ATTO 488 200 ug $394
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BHA11900876-5 SMC-159B-A594 ATTO 594 200 ug $394
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BHA11900876-6 SMC-159B-ALP Alkaline Phosphatase 200 ug $382
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BHA11900876-7 SMC-159B-APC APC 200 ug $393
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BHA11900876-8 SMC-159B-BI Biotin 200 ug $384
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BHA11900876-9 SMC-159B-FITC FITC 200 ug $376
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BHA11900876-10 SMC-159B-HRP HRP 200 ug $370
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BHA11900876-11 SMC-159B-PCP PerCP 200 ug $393
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BHA11900876-12 SMC-159B-RPE RPE 200 ug $386
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BHA11900876-13 SMC-159S Unconjugated 12 ug $44
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