Rabbit Anti-Human Cdc7 (pThr376) Polyclonal

Mfr.No.	SPC-934, SPC-934D, SPC-934D-A390, SPC-934D-A488, SPC-934D-A594, SPC-934D-BI, SPC-934D-FITC
Immunogen Species	Human
Alternative Names	CDC7 cell division cycle 7 like 1 Antibody, Cell division cycle 7 kinase Antibody, HsCdc7 Antibody, Hsk 1 Antibody, HuCDC 7 Antibody
Antigen	Cdc7
Categories	Antibodies
Cellular Localization	Nucleus
Certificate Of Analysis	A 1:250 dilution of SPC-934 was sufficient for detection of Cdc7 (pThr376) in 10 ug of human breast cancer cell (MCF7) lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human Cdc7 (pThr376) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Thr376 of human Cdc7 (AA373-379), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Thr376 of human Cdc7 (AA373-379)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	O00311
Accession Number	NP_003494
Gene Id	8317
Target	Cdc7 (pThr376)
Research Area	Cell Signaling, Protein Phosphorylation, Epigenetics and Nuclear Signaling, Cancer, Cell Cycle

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7/Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase a-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4. The kinase activity of the complex is regulated throughout the cell cycle, mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 63.888 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11911628-1	SPC-934D	Unconjugated	100 ul	$331	Add to Cart
BHA11911628-2	SPC-934D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11911628-3	SPC-934D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11911628-4	SPC-934D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11911628-5	SPC-934D-BI	Biotin	100 ul	$380	Add to Cart
BHA11911628-6	SPC-934D-FITC	FITC	100 ul	$375	Add to Cart

Cdc7 Antibody (pThr376) - SPC-934