Rabbit Anti-Human Chk1 (pSer345) Polyclonal

Mfr.No.	SPC-949, SPC-949D, SPC-949D-A390, SPC-949D-A488, SPC-949D-A594, SPC-949D-BI, SPC-949D-FITC
Immunogen Species	Human
Alternative Names	C85740 Antibody, Cell cycle checkpoint kinase Antibody, CHEK 1 Antibody, CHK1 checkpoint homolog (S. pombe) Antibody, CHK1_HUMAN Antibody, rad27 Antibody, Serine/threonine-protein kinase CHK1 Antibody
Antigen	Chk1
Categories	Antibodies
Cellular Localization	Cytoplasmic
Certificate Of Analysis	A 1:250 dilution of SPC-949 was sufficient for detection of Chk1 (pSer345) in 10 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human Chk1 (pSer345) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Ser345 of human Chk1 (AA342-348), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Ser345 of human Chk1 (AA342-348)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	O14757
Accession Number	NP_001265
Gene Id	1111
Target	Chk1 (pSer345)
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair, Chk1 / Chk2, Cancer, Cell Cycle, Protein Phosphorylation

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Chk1 is a protein-serine/threonine kinase required for checkpoint-mediated cell cycle arrest. It also subsequently activates DNA repair (i.e. homologous recombination) by phosphorylating RAD51 or FANCE in response to the presence of damage or unreplicated DNA. It regulates cell cycle progression via several mechanisms: 1) phosphorylation of certain proteins such as CDC25A, CDC25B and CDC25C, promoting their degradation; 2) phosphorylation and activation of p53; 3) phosphorylation and inactivation of TLK1, which consequently prevents TLK1-dependent phosphorylation of chromatin assembly factor ASF1A; 4) phosphorylation of histones and regulating transcription of genes involved in cell-cycle progression regulation; 5) phosphorylation of RB1 to promote its interaction with E2F transcription factors and subsequent cell cycle arrest
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 54.42 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11911898-1	SPC-949D	Unconjugated	100 ul	$331	Add to Cart
BHA11911898-2	SPC-949D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11911898-3	SPC-949D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11911898-4	SPC-949D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11911898-5	SPC-949D-BI	Biotin	100 ul	$380	Add to Cart
BHA11911898-6	SPC-949D-FITC	FITC	100 ul	$375	Add to Cart

Chk1 Antibody (pSer345) - SPC-949