Rabbit Anti-Human Chk2 (pThr68) Polyclonal

Mfr.No.	SPC-951, SPC-951D, SPC-951D-A390, SPC-951D-A488, SPC-951D-A594, SPC-951D-BI, SPC-951D-FITC
Immunogen Species	Human
Alternative Names	Cds1 homolog Antibody, Checkpoint kinase 2 Antibody, CHEK 2 Antibody, CHK2 checkpoint homolog Antibody, CHK2_HUMAN Antibody, hCds1 Antibody, LFS 2 Antibody, PP1425 Antibody, RAD 53 Antibody, Serine/threonine protein kinase Chk2 Antibody
Antigen	Chk2
Categories	Antibodies
Cellular Localization	Nucleus
Certificate Of Analysis	A 1:250 dilution of SPC-951 was sufficient for detection of Chk2 (pThr68) in 10 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human Chk2 (pThr68) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Thr68 of human Chk2 (AA65-71), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Thr68 of human Chk2 (AA65-71)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	O96017
Accession Number	NP_009125
Gene Id	11200
Target	Chk2 (pThr68)
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair, Chk1 / Chk2, Cancer, Cell Cycle, Protein Phosphorylation

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Chk2 is a protein kinase involved in cell cycle arrest in response to DNA damage. CHK2 is activated through phosphorylation by ATM and ATR at T68. Activated CHK2 phosphorylates and inhibits CDC25 a dual-specificity phosphatase required for progression through the cell cycle. CHK2 further causes cell cycle earrest through activation of a DNA repair protein BRCA1 and apoptosis factor PML. The role of CHK2 in the development of cancer is in a primarily tumour suppressor role through causing cell cycle arrest. It has been observed that inhibition of CHK2 through mutations in the regulatory FHA domain has been observed in low frequency in osteosarcomas and cancers of the ovary, lung and vulva. Another mutation, "1100delC" has been commonly found in breast cancers.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 60.915 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11911934-1	SPC-951D	Unconjugated	100 ul	$331	Add to Cart
BHA11911934-2	SPC-951D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11911934-3	SPC-951D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11911934-4	SPC-951D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11911934-5	SPC-951D-BI	Biotin	100 ul	$380	Add to Cart
BHA11911934-6	SPC-951D-FITC	FITC	100 ul	$375	Add to Cart

Chk2 Antibody (pThr68) - SPC-951