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Home / Primary Antibodies / Monoclonal Antibodies

HSP70 Antibody, Clone 5A5 - SMC-162

BHA11900936
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Email
[email protected]
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Swissprot P0DMV8/P0DMV9
Accession Number NP_005336.3
Gene Id 3303
Target HSP70
Clone No 5A5
Research Area Cell Signaling, Chaperone Proteins, Cancer, Tumor Biomarkers, Heat Shock, Protein Trafficking
Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:500), IP (1µg); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~70kDa. May detect HSP70, HSC70, Grp78 and HSP72.
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications AM, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; AM
Dilution WB (1:1000), ICC/IF (1:500), IP (1ug); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Balashova N. et al. (2005) J Biol Chem 280:2186-96.

2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17.

3. Rothman J. (1989) Cell 59: 591 -601.

4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.

5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294.

6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900936-1 SMC-162D Unconjugated 100 ug $311
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BHA11900936-2 SMC-162C Unconjugated 25 ug $156
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BHA11900936-3 SMC-162D-A390 ATTO 390 100 ug $365
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BHA11900936-4 SMC-162D-A488 ATTO 488 100 ug $363
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BHA11900936-5 SMC-162D-A594 ATTO 594 100 ug $363
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BHA11900936-6 SMC-162D-ALP Alkaline Phosphatase 100 ug $356
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BHA11900936-7 SMC-162D-APC APC 100 ug $362
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BHA11900936-8 SMC-162D-BI Biotin 100 ug $359
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BHA11900936-9 SMC-162D-FITC FITC 100 ug $354
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BHA11900936-10 SMC-162D-HRP HRP 100 ug $350
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BHA11900936-11 SMC-162D-PCP PerCP 100 ug $362
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BHA11900936-12 SMC-162D-RPE RPE 100 ug $360
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BHA11900936-13 SMC-162S Unconjugated 12 ug $44
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