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Home / Primary Antibodies / Polyclonal Antibodies

HSP70/HSC70 (pan) Antibody - SPC-311

BHA11907722
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+1 866.986.9598
Email
[email protected]
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Target HSP70
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Buffer Lyophilized, PBS pH7.4. For reconstitution add 100 µl of sterile water.
Dilutions WB (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5). Looking for more information on HSP70 Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~70kDa.
Storage -20ºC
Storage Buffer Lyophilized, PBS pH7.4. For reconstitution add 100 ul of sterile water.
Storage Temperature -20°C
Applications WB
Description WB
Dilution WB (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17.

2. Rothman J. (1989) Cell. 59: 591-601.

3. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887.

4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294.

5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11907722-1 SPC-311D Unconjugated 100 ug $356
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