Protein disulfide-isomerase, also known as the beta-subunit of prolyl 4-hydroxylase (P4HB), is an enzyme that in humans encoded by the P4HB gene. This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids A146-D363) was used as the immunogen for the PDI antibody.
Isotype
IgG
Predicted Reactivity
Human, Monkey, Mouse, Rat
Reactivity
Human, Monkey, Mouse, Rat
Recombinant
No
Subcellular Location
Cytoplasmic, cell membrane
Antigen
PDI
Uniprot
P07237
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Format
Purified
Purification
Antigen affinity purified
Storage
After reconstitution, the PDI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.