Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal

Mfr.No.	SPC-991, SPC-991D, SPC-991D-A390, SPC-991D-A488, SPC-991D-A594, SPC-991D-BI, SPC-991D-FITC
Immunogen Species	Human
Alternative Names	GTF2F1 Antibody, Transcription initiation factor IIF subunit alpha Antibody, 2810405L04Rik Antibody, BTF4 Antibody, C76800 Antibody, General transcription factor IIF 74 kDa subunit Antibody, MGC94148 Antibody, OTTHUMP00000237859 Antibody, RAP74 Antibody, T2FA_HUMAN Antibody, TF2F1 Antibody, GTF2F1 Antibody, TFIIF-alpha Antibody, Transcription initiation factor RAP74 Antibody
Antigen	TFIIF
Categories	Antibodies
Cellular Localization	Nucleus
Certificate Of Analysis	A 1:250 dilution of SPC-991 was sufficient for detection of TFIIF (pSer385 + pThr389) in 10 ug of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Ser385 and Thr389 of human TFIIF (AA382-392), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Ser385 and Thr389 of human TFIIF (AA382-392)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	P35269
Accession Number	NP_002087
Gene Id	2962
Target	TFIIF (pSer385 + pThr389)
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Polymerase Associated Factors, Pol II Transcription

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 58.24 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11912654-1	SPC-991D	Unconjugated	100 ul	$331	Add to Cart
BHA11912654-2	SPC-991D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11912654-3	SPC-991D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11912654-4	SPC-991D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11912654-5	SPC-991D-BI	Biotin	100 ul	$380	Add to Cart
BHA11912654-6	SPC-991D-FITC	FITC	100 ul	$375	Add to Cart

TFIIF Antibody (pSer385 + pThr389) - SPC-991