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E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit

BHE16300149

This product includes 400 ul of 10 x Buffer, 33 ul of 100 x Template, 33 ul of 100 x NTPs (50 mM ATP and 50 mM GTP) and 330 ul of 10 x fluorescence dye. It is for 100 assays of E. coli PNPase in a 384-well plate format. The assay This product includes all reagents except the enzyme.

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[email protected]
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Product Manual Datasheet
Protocols Description ASSAY PROTOCOL
The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl.
 
1. Reaction:
The total volume of each reaction mixture is 30 µl including 26.1 µl of H2O, 3 µl of 10 x Buffer, 0.3 µl of 100 x template, 0.3 µl of 100 x PNPase and 0.3 µl of 100 x ADP. Incubate the reaction mixture at 37°C for 60 min.
 
2. Detection:
Dilute the 10x fluorescence dye with water to make the 1 x dye. Mix 30 µl of the 1 x dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm in 5 min.
Combination SKU Supplier No. Specifications Size Your price Quantity
BHE16300149-1 PNP100K enzyme not included 100 assays $348
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BHE16300149-2 PNP100KE enzyme included 100 assays $556.13
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