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Home / Assay Kits

E. coli RNA polymerase Assay Kit

SKU : BHE16300151

This product includes 400 ul of 10 x Buffer, 33 ul 100 x DNA, 33 ul of 100x NTPs, 350 ul of 10 x fluorescence dye. The assay This product includes all reagents except the enzyme. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.

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Mfr.No.	RPA100K, RPA100KE
Description	The bacterial RNA polymerase is responsible for biosynthesis of mRNA, tRNA and rRMA in the cells. The holoenzyme of bacterial RNA polymerases contain multiple subunits with a molecular mass of about 390 kDa. The bacterial RNA Polymerase Assay Kit is based on measurement of the RNA molecules synthesized by the RNA polymerase using a DNA template. The assay can be performed in 384-well plate format for high throughput screening of RNA polymerase inhibitors. The E. coli RNA Polymerase Assay Kit (Catalog number RPA100K) includes 400 µl of 10 x Buffer, 33 µl 100 x DNA, 33 µl of 100x NTPs, 350 µl of 10 x fluorescence dye. The assay kit includes all reagents except the enzyme. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.
Drug target assays	Antibacterial drug targets

Product Manual	Datasheet
Protocols Description	Assay Protocol The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl. 1. Reagent preparation: (1) 10 x DNA template: dilute the 100 x DNA template10-fold with water. (2) 10 x enzyme: dilute the 100 x RNA polymerase 10-fold with the 1 x Buffer. (3) 10 x NTP mix: dilute the 100 x NTP mix (50 mM) 10-fold with water. (4) 1 x fluorescence dye: dilute the 10 x fluorescence dye 10-fold with water. 2. Reaction: The total volume of each reaction mixture is 30 µl including 18 µl of H2O, 3 µl of 10 x Buffer, 3 µl of 10 x DNA template, 3 µl of 10 x enzyme, 3 µl of 10 x NTP mix. Incubate the reaction mixture in a standard black 384-well plate (Matrix 4318) at 37°C for 60 min. 3. Detection: Add 30 µl of the 1 x fluorescence dye into the 30 µl of the reaction mixture. Incubate for 5 min. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300151-1	RPA100K	enzyme not included	100 assays	$348		Add to Cart
BHE16300151-2	RPA100KE	enzyme included	100 assays	$583.94		Add to Cart

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