Gel-based Human Topoisomerase II DNA Decatenation Assay Kit Plus

Mfr.No.	HDD100KEG
Description	Anticancer Drug Target. Human DNA topoisomerase II alpha converts the concatenated DNA into decatenated DNA. ProFoldin offers the Human DNA topoisomerase II assays in two assay formats: Spin-column-based DNA decatenation assay and 96-well filter platebased DNA decatenation assay. In the spin-column-based DNA decatenation assay, the decatenated DNA is separated from the concatenated DNA by a quick and easy spin-column process. The concatenated DNA stays on the column, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence. In the 96-well filter platebased DNA decatenation assay, the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm). This product includes 300 ul of 10 x assay buffer, 200 ul of 10 x concatenated DNA (30 ug/ml), 200 ul of 10 x ATP, 200 ul of 0.5 M EDTA, 15 ul of 200 x human topoisomerase II alpha (10 U/ul), 1 ml of enzyme dilution buffer and 500 ul of 5 x gel-loading buffer for 100 assays of human DNA decatenation reactions.
Drug target assays	Anticancer drug targets

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300014	HDD100KEG	100 assays	$447.10		Add to Cart