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Home / Assay Kits

Human DNA polymerase beta assay kit

BHA16300003

This product includes all the assay reagents except the enzyme for 100 assays of human DNA polymerase beta reactions in a 96-well plate format.

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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
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Product Manual Datasheet
Protocols Description Assay protocol
The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 50 µl and the final assay volume is 100 µl.
 
1. Reagent preparation:
(1) 10 x DNA: Dilute the 100 x DNA 10-fold with water. Each assay uses 3 µl of 10 x DNA.
(2) 10 x enzyme: Dilute the 100 x Human DNA polymerase beta 10-fold with the 1 x assay buffer. Each assay uses 3 µl of 10 x enzyme.
(3) 10 x dNTP mix: Dilute the 100 x dNTP mix 10-fold with water. Each assay uses 3 µl of 10 x dNTP mix.
(4) 1 x dye: Dilute the 2 x fluorescence dye 2-fold with 50 mM EDTA. Each assay uses 30 µl of 1 x dye.
 
2. Reaction:
The total volume of each reaction mixture is 30 µl including 18 µl of H2O, 3 µl of 10 x Buffer, 3 µl of 10 x DNA template, 3 µl of 10 x enzyme, 3 µl of 10 x dNTP mix. Incubate the reaction mixture at 37°C for 60 min.
 
3. Detection:
Mix 30 µl of the 1 x fluorescence dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.
Combination SKU Supplier No. Specifications Your price Quantity
BHA16300003-1 DPB100K enzyme not included $348
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BHA16300003-2 DPB100KE enzyme included $560.35
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