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Home / Assay Kits

Human DNA Polymerase Gamma Assay Kit

SKU : BHA16300005

This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

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Mfr.No.	DPG100K, DPG100KE
Description	Human DNA polymerase gamma is the only DNA polymerase in human mitochondria and is responsible for the DNA replication, recombination and repairing in human mitochondria. It plays critical roles in mitochondrial diseases and aging. Some antiviral nucleoside analogs were reported to inhibit DNA polymerase gamma after intracellular phosphorylation and cause severe chronic toxicity. The human DNA polymerase gamma assay is based on measurement of the DNA molecules synthesized by the DNA polymerase. The assay is performed in a 384-well or 96-well plate format. The assay can be used for detection of DNA polymerase gamma activity and high throughput screen of human DNA polymerase gamma inhibitors. The Human DNA Polymerase Gamma Assay Kit (Catalog No. DPG100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template (3 µM), 33 µl of 100 x dNTP mix (1 mM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.
Detection Method	/
Drug target assays	Anticancer drug targets

Product Manual	Datasheet
Protocols Description	Assay protocol The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl. 1. Reagent preparation: (1) 10 x DNA: Dilute the 100 x DNA 10-fold with water. Each assay uses 3 µl of 10 x DNA. (2) 10 x enzyme: Dilute the 100 x Human DNA polymerase gamma 10-fold with the 1 x assay buffer. Each assay uses 3 µl of 10 x enzyme. (3) 10 x dNTP mix: Dilute the 100 x dNTP mix 10-fold with water. Each assay uses 3 µl of 10 x dNTP mix. (4) 1 x dye: Dilute the 2 x fluorescence dye 2-fold with 50 mM EDTA. Each assay uses 30 µl of 1 x dye. 2. Reaction: The total volume of each reaction mixture is 30 µl including 18 µl of H2O, 3 µl of 10 x Buffer, 3 µl of 10 x DNA template, 3 µl of 10 x enzyme, 3 µl of 10 x dNTP mix. Incubate the reaction mixture at 37°C for 60 min. 3. Detection: Mix 30 µl of the 1 x fluorescence dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.

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Combination SKU	Supplier No.	Specifications	Your price	Quantity
BHA16300005-1	DPG100K	enzyme not included	$465.97		Add to Cart
BHA16300005-2	DPG100KE	enzyme included	$583.94		Add to Cart

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