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Home / Assay Kits

Human poly (ADP-ribose) polymerase-1 assay kit

SKU : BHE16300020

This product includes the assay buffer, DNA template and fluorescence dye for 100 assays of human PARP-1 in a 96-well plate format.

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Mfr.No.	PAR100K, PAR100KE
Description	Poly (ADP-ribose) polymerase-1 (PARP-1) responses to DNA damage and synthesizes poly ADP-ribose (PAR) that is transferred to itself and a variety of acceptor proteins. Excessive activation of PARP-1 not only depletes NAD+ but also generates toxic PAR that lead to cell death. PARP-1 is an anti-cancer drug target. It is also a potential drug target for other diseases such as stroke, ischemia and reperfusion where high-level DNA damage occurs. The human PARP-1 assay is based on measurement of its product PAR (poly ADP-ribose) that binds to a fluorescent dye and enhances its fluorescent signal. The Human Poly (ADP-ribose) Polymerase-1 Assay Kit (catalog number PAR100K) includes 800 µl of 10 x the assay buffer, 55 µl of 100x NAD+ , 55 µl of 100x DNA and 220 µl of 100 x fluorescence dye for 100 assays of human PARP-1 in a 96-well plate format. The kit does not include Human PARP-1.
Drug target assays	Anticancer drug targets

Product Manual	Datasheet
Protocols Description	Assay Protocol 1.Reagent preparation: 10 x DNA: dilute the 100 x DNA 10 –fold with water 10 x enzyme: dilute the 400 x Human PARP-1 40 –fold with 1 x Buffer 1 mM NAD+ : dilute the 10 mM stock 10 – fold with water 1 x fluorescence dye: dilute the 100 x fluorescence dye 100-fold with water 2. Reaction: The total volume of each reaction mixture is 50 µl including: 30 µl of H2O, 5 µl of 10 x buffer, 5 µl of 10 x DNA, 5 µl of 10 x human PARP-1, 5 µl of 1 mM NAD+ . Incubate the reaction mixture at 37°C for 60 min. 3. Detection: Add 200 µl of the 1 x fluorescence dye into the 50 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300020-1	PAR100K	enzyme not included	100 assays	$348		Add to Cart
BHE16300020-2	PAR100KE	enzyme included	100 assays	$780.83		Add to Cart

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