Loading ....
Contact us to order
Tel
+1 866.986.9598
Elabscience Biotechnology Co.,Ltd
view supplier detailsCredit card payments now incur a 3% fee.
Elabscience Biotechnology Co.,Ltd
view supplier detailsCredit card payments now incur a 3% fee.
| Mfr.No. | E-BC-K061-S |
| Background | Hydroxyproline accounts for 13.4% in collagen, minute quantity in elastin, and not exists in other proteins. And collagen mostly exists in skin, tendon, cartilage, blood vessel, etc, so the quantity and energy of hydroxyproline can reflect collagen metabolic status of connective tissue diseases. Surgical trauma can lead to the increase of hydroxyproline excretion amount in urine. Fiber can increase through inflammation because of high fat, low protein, alcoholism and poison, malnutrition or hepatocellular degeneration and necrosis, then segments hepatic lobule and results in liver cirrhosis. When affected with hepatic fibrosis, collagenous fiber is the main increasing component in liver. And hydroxyproline is specific to collagenous fiber. So the content of liver collagen can be calculated by measuring the content of liver hydroxyproline to reflect the degree of hepatic fibrosis. The decomposition of collagen fiber primarily depends on the function of collagenase and other proteases, whose decomposition product - hydroxyproline is excreted in the urine. So measuring the content of urine hydroxyproline can reflect the degradation situation of collagen. On the one hand, measuring the content of hydroxyproline in tissue and urine can judge the degree of fibrosis; on the other hand, it can screen the drugs of prevention and treatment. |
| Description | This kit can be used for detection of hydroxyproline content in samples, such as animal serum (plasma), tissue, cultured cells and solution, body fluids etc. |
| Description | Biochemical |
| Assay Type | Biochemical kit |
| Detection Instrument | Spectrophotometry (Visible range) |
| Detection Method | Colorimetric method |
| Detection Range | 0.01-20μg/ml |
| Experiment Instruments | Spectrophotometry (Visible range) |
| Inter-cv (%) | 4.65 |
| Intra-cv (%) | 2.2 |
| Manual | 
|
| Recovery | 102 |
| Sensitivity | 0.01μg/ml |
| Manual | 
|
