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Home / Assay Kits

M. Tuberculosis ClpP Protease Assay Kit

SKU : BHE16300146

This product includes 3100 ul of Assay Buffer and 310 ul of 10 x Substrate and 3500 ul of Stop solution. It is for 100 assays in 384-well plate format

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ProFoldin

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Mfr.No.	TBP100K, TBP100KE
Description	The disease of tuberculosis (TB) is a serious threat to global public health. It is estimated that infection of M. tuberculosis (Mtb) causes more than 1 million deaths annually. The protein degradation enzyme ClpP protease of Mtb composed of subunits P1 and P2 is a proven drug target against Mtb. ProFoldin’s high throughput assay for Mtb ClpP protease is based on cleavage of a labeled peptide substrate1 that generates fluorescence at 460 nm (excitation at 380 nm). The assay can be performed in a 384-well or 96- well plate format for tests of Mtb ClpP protease activities and throughput screening of inhibitors against Mtb. The M. Tuberculosis ClpP Protease Assay Kit (Catalog No. TBP100K) includes 3100 µl of Assay Buffer and 310 µl of 10 x Substrate and 3500 µl of Stop solution. It is for 100 assays in 384-well plate format. All assay reagents except the enzyme are included.
Drug target assays	Antibacterial drug targets

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl. 1. Plate with inhibitors Add 0.6 µl of 50 x inhibitor in DMSO into each well of a 384-well plate. Use DMSO only for no-inhibitor control. 2. Mtb ClpP assay (1) For each 10 assay reactions, prepare a premix by mixing 275 µl of Assay Buffer and 3.1 µl of 100 x Mtb ClpP1P2. (2) Add 26.4 µl of the prmix into the wells with 0.6 µl of 50 x inhibitor or DMSO and incubate the solution at 37°C for 20 min. (3) Freshly dilute the 100 x Substrate with the assay buffer 10 fold to make the 10 x Substrate. Add 3 µl of 10 x Substrate and incubate the assay reaction at 37°C. (4) For continues assays, read the fluorescence emission at 460 nm with excitation at 380 nm at various time points from zero to 60 min. For endpoint assays, incubate the assay reaction solution at 37°C for 60 min, then add 30 µl of Stop solution and read the read the fluorescence emission at 460 nm with excitation at 380 nm.

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Combination SKU	Supplier No.	Specifications	Your price	Quantity
BHE16300146-1	TBP100K	enzyme not included	$348		Add to Cart
BHE16300146-2	TBP100KE	enzyme included	$583.94		Add to Cart

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