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Home / Assay Kits / ELISA Kits

Pro Ligation-Free Cloning Kit (20 reactions)

SKU : BHE10900004

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E086
Description	The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs. Testimonial: "I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit." - Jianfeng Lan, The Buck Institute
Featured	One-step cloning: No more time-consuming, multi-step traditional cloning that depnds on available restrict sites or compatible ends Scar-free cloning: No unwanted/extra base pairs in the inserts Easy-to-customize: Clone in promoter, gene, tag, multi-fragment or whiole vector constructs in one step Ready for downstream applications: Ligase-free reaction, no gel extraction or column purification steps needed Robust assembly: Better performance for difficult, multi-fragment assemblies such as CRISPR multiplex sgRNA vector assembly Increased thermostability: High colony yields
Notes	Applications: Rapid multi-fragment cloning and assembly of whole vector constructs Clone in a promoter, gene, and tag, all in one step Rapid assembly of CRISPR multiplex gRNA vectors in one step

Reference	Sherman, S. L. . "Cofactor-Independent Pinacolase Directs Non-Diels-Alderase Biogenesis of the Brevianamides" : ().

Product Manual	Quick Start Protocol Pro Ligation-Free Cloning Kit Manual

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SKU	Supplier No.	Your price	Quantity
BHE10900004	E086	$120		Add to Cart

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