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Home / Assay Kits

Beta Lactamase Assay Kit

SKU : BHE16300144

This product includes 550 ul of 10 x Buffer and 110 ul of 50 x penicillin G and 260 ul of 10 x fluorescence dye.

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ProFoldin

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Mfr.No.	LAC100K, LAC100KE
Description	Resistance to β-lactam antibiotics such as penicillins, cephalosporins, cephamycins and carbapenems are due to hydrolysis of the four-atom ring known as β-lactam by β-lactamases (penicillinase) produced by Gram-negative bacteria. Inhibition of β-lactamases prevents bacterial degradation of beta-lactam antibiotics. ProFoldin’s high throughput assay for β-lactamases is based on binding of the dye with the hydrolyzed β-lactam that generates fluorescence at 535 nm (excitation at 485 nm). The assay can be performed in a 384-well or 96-well plate format for tests of β-lactamase activities and throughput screening of inhibitors against β-lactamases. The assay is not compatible with samples containing free amino acids or buffers containing DTT or EDTA. The Beta Lactamase Assay Kit (Catalog No. LAC100K) includes 550 µl of 10 x Buffer, 110 µl of 50 x penicillin G and 260 µl of 10 x fluorescence dye. The 10 x Buffer is composed of 100 mM HEPES, pH 7.5, 500 mM KCl. The kit is for 100 assays of β-lactamases in a 96-well plate or 384-well plate format. All assay reagents except the enzyme are included.
Drug target assays	Antibacterial drug targets

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL A 384-well plate (plate type Matrix 4318 or alike) or 96-well plate (plate type: Costar 3915 or alike) may be used for the fluorescence-based assay. The reaction volume is 50 µl and the final assay volume is 75 µl. 1. Reaction:Prepare each reaction solution by mixing 43.5 µl of H2O, 5 µl of 10 x Buffer, 1 µl of 50 x penicillin G and 0.5 µl of 100 x β-lactamase. Incubate the reaction mixture at 37°C for 60 min. 2. Detection: Dilute the 10 x fluorescence dye with water to make the 1 x dye. Mix 25 µl of the 1 x dye with 50 µl of the reaction mixture at the end of the reaction. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm in 5 min.

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Combination SKU	Supplier No.	Specifications	Your price	Quantity
BHE16300144-1	LAC100K	enzyme not included	$230.04		Add to Cart
BHE16300144-2	LAC100KE	enzyme included	$443.78		Add to Cart

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