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E. coli D-Ala-D-Ala ligase - for 100 assays

BHE16300167

This product includes 33 ul of 200 nM E. coli D-Ala:D-Ala ligase.

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+1 866.986.9598
Email
[email protected]
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Product Manual Datasheet
Protocols Description Assay protocol using the E. coli D-alanine: D-alanine ligase assay kit
The following assay protocol is based on the 384-well plate assay format. The reaction volume is 30 µl and the final assay volume is 75 µl. For 96-well plate assays, the reaction volume is 60 µl and the final assay volume is 150 µl. For detection using a cuvette, the reaction volume is 400 µl and the final assay volume is 1000 µl.
 
1. Reagent preparation:
For each 10 assay reactions,
(1) Prepare 297 µl of premix composed of 261 µl of H2O, 33 µl of 10 x Buffer and 3.3 µl of 100 x E. coli D[1]Ala:D-Ala ligase.
(2) Prepare 33 µl of 10 x Enzyme substrate by mixing 3.3ml of 100 x Enzyme substrate with 29.7ml of water.
 
2. Reaction:
Mix 27 µl of the premix with 3 µl of the 10 x Enzyme substrate in each well. Incubate the reaction mixture at 37°C for 60 min.
 
3. Detection:
Add 45 µl of the Dye MPA3000 into the 30 µl of the reaction mixture. Incubate for 5 min. Measure the light absorbance at 650 nm.
SKU Supplier No. Size Your price Quantity
BHE16300167 DDL-100EC 100 assays $386.75
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