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Lipid Bilayer Permeability Disruption Assay Kit

BHE16300405

This product includes 1.25 ml of liposomal dye, 1.25 ml of color development solution, 7.5 ml of buffer and 1 ml of DMSO. It is for assays of 100 samples using a 96-well plate.

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[email protected]
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Product Manual Datasheet
Protocols Description PROTOCOL
The following protocol is based on using a 96-well plate fluorescence plate reader (emission 580 nm, excitation 540 nm). The total sample volume is 100 µl. Cuvettes may also be used for fluorescence measurement if the sample volume is adjusted accordingly.
 
1. Temperature effect on bilayer permeability
Heating disrupts bilayer permeability and releases the liposome-encapsulated dye. To test the temperature effect, in each test tube, add 75 µl of Buffer, 12.5 µl of liposomal dye and 12.5 µl of color development solution. Incubate the tubes at different temperatures (e. g. boiling water bath, room temperature, etc) for 5 min. Transfer the samples into wells of a black 96-well plate and read the fluorescence at 580 nm (Excitation at 540 nm) in 10 min.
 
2. Control experiment of bilayer permeability disruption by DMSO
DMSO completely disrupts bilayer permeability and releases the liposome-encapsulated dye. In a well of a black 96-well plate, add 75 µl of DMSO, 12.5 µl of liposomal dye and 12.5 µl of color development solution. Incubate the sample at room temperature for 15 min and read the fluorescence at 580 nm (Excitation at 540 nm).
 
3. Disruption of bilayer permeability by compounds
Some compounds may also disrupt bilayer permeability and release the liposome-encapsulated dye. In a well of a black 96-well plate, add the compound solution and Buffer to make a volume of 75 µl then add 12.5 µl of liposomal dye. Incubate the tube at a proper temperature for a selected time period. Finally add 12.5 µl of color development solution and incubate the sample for 10 min. Read the fluorescence at 580 nm (Excitation at 540 nm).
SKU Supplier No. Size Your price Quantity
BHE16300405 BPD100K 100 assays $406.99
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