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Home / Assay Kits / Biochemical Kits

Micro desalting spin column set

SKU : BHE16300403

This product includes 50 pre-packed spin-columns. Each column is to desalt 25 ul of sample. The volume of desalted sample is 25 to 30 ul.

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ProFoldin

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+1 866.986.9598

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Mfr.No.	MDC050
Description	ProFoldin desalting columns are designed to remove small molecules like salts, free enzyme substrates or ligands from a protein or DNA solution. After desalting, the protein or DNA sample is in a low-salt buffer composed of 10 mM Tris-HCl, pH 7.5. The columns can also be used for buffer exchange of protein or DNA samples to a desired buffer which is used to pre-equilibrate the column. The principle of desalting is size-exclusion chromatography with a molecular cut-off of 5 kDa. The residual salt concentration in the desalted solution is less than 2 % of the original salt concentration. The protein recovery yield is 98 % or higher. The Micro Desalting Spin Columns Set (Catalog number: MDC050) contains 50 pre-packed spin-columns. Each column is to desalt 25 to 50 µl of sample.

Product Manual	Datasheet
Protocols Description	DESALTING OR BUFFER EXCHANGE PROCEDURE 1. Spin the pre-packed columns at 3200 rpm (1000 x g) for 1 min using a bench-top microcentrifuge to set down the resin. Remove the column bottom tips and caps. Place the columns into 1.5 ml-eppendorf tubes and spin the columns at 3200 rpm for 2 min. 2. If the protein is to be buffer-exchanged to a specific buffer rather than 10 mM Tris-HCl, pH 7.5, add 250 µl of the specific buffer and spin the columns 3200 rpm for 2 min. 3. Transfer each column into a clean labeled 1.5-ml eppendorf tube. 4. Load 25 µl of the sample onto each spin column and spin the columns at 3200 rpm for 4 min. Save the desalted samples.

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300403	MDC050	50 columns	$234.76		Add to Cart

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