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MicroMolar Oxaliplatin Assay Kit

BHE16300273

This product includes 4 ml of Reagent A, 0.080 ml of 50 x Reagent B, and 2 ml of 10 x Reagent C. It is for 200 assays using 96-well plates. Cuvettes may also be used for measurements.

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Product Manual Datasheet
Protocols Description ASSAY PROTOCOL
The following assay protocol is based on using a 96-well plate for the measurement.  The sample volume is 100 µl and the final assay volume is 240 µl. The assay reactions and measurement are on a transparent 96-well plate. It is a high throughput assay format.
 
For assays using cuvette, the sample volume is 400 µl and the final assay volume is 960 µl. The assay reactions are carried out in eppendorf tubes or test tubes. The samples are transferred into a cuvette for measurement of the light absorbance.
 
STANDARD CURVE
1. Sample preparation: Prepare 100 µl of oxaliplatin solutions in the wells of a transparent 96-well plate with a two-fold serial dilution from 0.25 mg/ml (0.63 mM) to zero in water. For each 10 assays, dilute 20 µl of 10 x Reagent B with 200 µl of water to make 1 x Reagent B; dilute 110 µl of the 10 x Reagent C with 1 ml of water to make 1 x Reagent C.
 
2. Assay: Into the wells with 100 µl of oxaliplatin solutions, add 20 µl of Reagent A and 20 µl of 1 x Reagent B. Incubate the mixtures at 65°C for 30 min. Add 100 µl of 1 x Reagent C into each well. Read the light absorbance at 440 nm (A440)
 
3. Data Analysis: Plot the A440 values and the Oxaliplatin concentration [Oxaliplatin] to generate the linear standard curve.
 
                                                          A440 = a [Oxaliplatin] + b
 
Where the A440 values are from experimental data, the a and b values are from the linear fitting between the A440 values and the Oxaliplatin concentrations.
 
UNKNOWN SAMPLES
Follow the same procedure to measure the light absorbance A440 values from the unknown samples. Calculate the Oxaliplatin concentrations in the unknown samples using the A440 values from the unknown samples and the a and b values from the standard curve.
 
                                                        [Oxaliplatin] = (A440 – b) / a
 
SKU Supplier No. Size Your price Quantity
BHE16300273 OPT200 200 assays $293.74
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