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Home / Biochemicals / Small Molecules

4-ONE

SKU : BHB21300255

An Activator of TRPA1 Channel

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Alomone Labs

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Mfr.No.	O-145, O-145_10 mg, O-145_25 mg, O-145_5 mg, O-145_50 mg
Description	4-ONE (#O-145) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C9H14O2.
Synonyms	Synthetic
Background	4-ONE is a lipid peroxidation product that acts as a TRPA1 (transient receptor potential ankyrin 1) channel activator and displays an EC50 value of 1.9 uM1. The compound contains an electrophilic unsaturated carbonyl moiety and forms adducts with lysine, histidine, and cysteine residues1. 4-ONE is rich in beta-sheet structures and is considered to be a reactive aldehyde that may be also cytotoxic2.A study conducted with 4-ONE showed that the compound is able to trigger unilateral mechanical hyperalgesia and vasodilatation in mice and also triggersTRPA1-independent effects that relate to edema formation and pain3.TRPA1 is a non-selective cation channel expressed in the small neurons of the dorsal root and trigeminal ganglia. TRPA1 channels play an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds4.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	1-100 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
Cas Number	103560-62-9
Molecular Weight	154.2
Solubility	Supplied in oil form. Soluble in DMSO, methanol, ethanol.Centrifuge all products before use (10000 x g 5 min).
Target	TRPA1 channels
Activity	4-ONE (4-Oxo-2-nonenal) is a lipid peroxidation product with multiple biological activities, shown to activate TRPA1 channels (EC50 = 1.9 uM)1.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: (E)-4-oxonon-2-enal.

Purity	>97%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: -20°C.

Product Manual	SDS

Reference	Andersson, D.A. et al. (2008) J. Neurosci. 28, 2485. Nasstrom, T. et al. (2011) Free Radic. Biol. Med. 50, 428. Graepel, R. et al. (2011) J. Pharmacol. Exp. Ther. 337, 117. Klionsky, L. et al. (2007) Mol. Pain 3, 39. Andersson, D.A. et al. (2008) J. Neurosci. 28, 2485.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300255-1	O-145_10 mg	10 mg	$235		Add to Cart
BHB21300255-2	O-145_25 mg	25 mg	$509		Add to Cart
BHB21300255-3	O-145_5 mg	5 mg	$134		Add to Cart
BHB21300255-4	O-145_50 mg	50 mg	$811		Add to Cart

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