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Home / Biochemicals / Small Molecules

5-Hydroxyindole

SKU : BHB21300169

A Modulator of 5-HT3 Receptors

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Alomone Labs

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Mfr.No.	H-115, H-115_1 g, H-115_250 mg
Description	5-Hydroxyindole (#H-115) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C8H7NO.
Synonyms	Synthetic
Background	Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. 5-HT3 receptor is a ligand-gated ion channel receptor of the cys-loop channel family. It is a pentamer with five subunits surrounding a central pore1.5-Hydroxyindole (5-indolol) is a synthetic, allosteric modulator of 5-HT3 channels. It has an effective concentration of 100 uM - 1 mM for 5-HT3A. 5-Hydroxyindole was tested in N1E-115 (neuroblastoma cells) and does not seem to elicit 5-HT receptor mediated currents on its own. However, in the presence of 5-hydroxyindole the effect of 5-HT in the same cells was significantly altered. Peak current amplitude was increased and desensitization of the 5-HT3 receptor was slowed down compared to cells solely infused with 5-HT. The effects of 5-hydroxyindole take place rapidly and are quickly reversed on washing with an external solution. Similar results can be achieved when infusing cells with other 5-HT receptor agonists. 5-Hydroxyindole enhances the peak amplitude of inward currents induced by infusion with the various 5-HT3 receptor agonists by 30-94% and slows down desensitization 2.5 - 5 fold. 5-Hydroxyindole can be used as a tool to selectively modify the desensitization of the 5-HT3 receptor2.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	100 uM - 1 mM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.
Cas Number	1953-54-4
Molecular Weight	133.15
Solubility	100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Target	5-HT3 receptors
Activity	5-Hydroxyindole is a 5-HT3 receptor allosteric modulator which slows the desensitization of the channel1.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 1H-indol-5-ol.

Purity	>97%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to four weeks at 4°C or three months at -20°C.

Product Manual	SDS

Reference	Hannon, J and Hoyer, D. (2008) Behav. Brain Res. 195, 198. Kooyman, A.R. et al. (1993) Br. J. Pharmacol. 108, 287. Kooyman, A.R. et al. (1993) Br. J. Pharmacol. 108, 287.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300169-1	H-115_1 g	1 g	$66		Add to Cart
BHB21300169-2	H-115_250 mg	250 mg	$50		Add to Cart

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