JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Biochemicals / Small Molecules

Benzamil hydrochloride

SKU : BHB21300058

An Inhibitor of Na+/Ca2+ Exchangers and a Blocker of ASIC, TRPP3 and TRPA1 Channels

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Alomone Labs

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	B-160, B-160_10 mg, B-160_25 mg, B-160_50 mg
Description	Benzamil hydrochloride (#B-160) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C13H15Cl2N7O.
Synonyms	Synthetic
Background	Benzamil is a potent amiloride analog that acts as an inhibitor of Na+/Ca2+ exchangers and a blocker of ASIC/Deg/ENaC, TRPP3, and TRPA1 channels displaying IC50 of 0.1, 1.1 and 55 uM respectively1-3.Benzamil is commercially available as an inhibitor for acid sensing ion channels (ASICs) belonging to the Deg/ENaC family. The compound is also well known as a diuretic compound that is used for the management of congestive heart disease or hypertension. Intrathecal administration of benzamil demonstrates antinociceptive effects in several pain models in animals and humans through the inhibition of acid sensing ion channels4.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	1-300 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity
Cas Number	161804-20-2
Molecular Weight	356.21
Solubility	Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).
Target	Na+/Ca2+ exchangers, ASIC/ENaC, TRPP3, TRPA1 channels
Activity	Benzamil is a potent amiloride analogue, an inhibitor of the Na+/Ca2+ exchanger (IC50 = 0.1 uM)1, a blocker of ASIC/Deg/ENaC channels2, of TRPP3 channels (IC50 = 1.1 uM)3 and of TRPA1 channels (IC50 = 55 uM)4.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 3,5-diamino-N-(N'-benzylcarbamimidoyl)-6-chloropyrazine-2-carboxamide;hydrochloride.

Purity	>99%
Form	Lyophilized Powder
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: Up to three months at -20°C.

Product Manual	SDS

Reference	Fischer, K.G. et al. (2002) Nephrol. Dial. Transplant 17, 1742. Dai, X.Q. et al. (2007) Mol. Pharmacol. 72, 1576. Banke, T.G. (2011) Brain Res. 1381, 21. Jeong. S. et al. (2013) J. Korean Med. Sci. 28, 1238. Fischer, K.G. et al. (2002) Nephrol. Dial. Transplant 17, 1742. Taruno, A. and Marunaka, Y. (2010) Cell Physiol. Biochem. 26, 925. Dai, X.Q. et al. (2007) Mol. Pharmacol. 72, 1576. Banke, T.G. (2011) Brain Res. 1381, 21.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300058-1	B-160_10 mg	10 mg	$66		Add to Cart
BHB21300058-2	B-160_25 mg	25 mg	$129		Add to Cart
BHB21300058-3	B-160_50 mg	50 mg	$213		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.