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Home / Biochemicals / Small Molecules

Forskolin

SKU : BHB21300144

An Adenylate Cyclase Activator

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Alomone Labs

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Mfr.No.	F-500, F-500_10 mg, F-500_100 mg, F-500_25 mg, F-500_5 mg, F-500_50 mg
Description	Forskolin (#F-500) is a highly pure, natural, and biologically active compound.
Molecular Formula	C22H34O7.
Synonyms	Natural
Background	Forskolin is a cell permeable diterpenoid isolated from Coleus forskohlii. Forskolin has a unique ability to stimulate adenylate cyclase activity and increase cyclic adenosine monophosphate (cAMP)1-10.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	5-10 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	66575-29-9
Molecular Weight	410.5
Solubility	DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Activity	Forskolin increases cAMP levels by activating adenylate cyclase1.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Cited Application: Electrophysiology Chemical Name: (3R-(3a,4ab,5b,6b,6aa,10a,10ab,10ba))-5-(Acetyloxy)-3-e thenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pent amethyl-1H-naphtho(2,1-b)pyran-1-one.

Purity	>97% (HPLC)
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to four months at 4°C.

Product Manual	SDS

Reference	De Souza, N.J. (1993) J. Ethnopharmacol. 38, 177. Insel, P.A. et al. (2003) Cell Mol. Neurobiol. 23, 305. Hoffman, B.B. et al. (1987) Horm. Metab. Res. 19, 358. Mills, I. et al. (1984) Endocrinology 115, 1066. Christenson, J.T. et al. (1995) Vasa 24, 56. Hermansen, K. (1985) Endocrinology 116, 2251. Metzger, H. et al. (1981) Arzneimittelforschung 31, 1248. Schlepper, M. et al. (1989) Basic Res. Cardiol. 84 Suppl 1, 197. Hartzell, H.C. et al. (1992) Mol. Pharmacol. 41, 880. Li, X. et al. (1995) Am. J. Physiol. 269, C986. Awad, J.A. et al. (1983) J. Biol. Chem. 258, 2960.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300144-1	F-500_10 mg	10 mg	$85		Add to Cart
BHB21300144-2	F-500_100 mg	100 mg	$287		Add to Cart
BHB21300144-3	F-500_25 mg	25 mg	$117		Add to Cart
BHB21300144-4	F-500_5 mg	5 mg	$66		Add to Cart
BHB21300144-5	F-500_50 mg	50 mg	$168		Add to Cart

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